Objective:To construct a set of rationality evaluation index system of medical service price items,so as to provide references and tools for improving the mechanism of finding abnormal medical service price items and the mechanism of monitoring and evaluating medical service price items.Methods:Literature Review,focus group discussion,Delphi method and Analytic Hierarchy Process were used to determine the evaluation indexes and their weights.Results:The rationality evaluation index system of medical service price items included 5 primary indexes,10 secondary indexes and 22 third-level indexes.According to the weight,primary indexes were cost compensation(0.253),clinical use(0.227),price comparison relationship(0.225),reform guidance(0.164)and technical evaluation(0.131).The positive coefficient of experts,1evel of authority and coordination of opinions were confirmed.The items whose specific single index score was zero or whose total score was below the threshold were considered as abnormal items.Conclusion:It constructed a set of rationality evaluation index system of medical service price items,which has high scientificity and reliability.Due to the prerequisites for obtaining the basic data required for the evaluation,it is recommended that a pilot project be carried out in the pilot areas of deepening the reform of medical service prices for the purpose of further verifying and optimizing the index system.

ObjectiveThe detection of RNA single nucleotide polymorphism (SNP) is of great importance due to their association with protein expression related to various diseases and drug responses. At present, splintR ligase-assisted methods are important approaches for RNA direct detection, but its specificity will be limited when the fidelity of ligases is not ideal. The aim of this study was to create a method to improve the specificity of splintR ligase for RNA detection. MethodsIn this study, a dual-competitive-padlock-probe (DCPLP) assay without the need for additional enzymes or reactions is proposed to improve specificity of splintR ligase ligation. To verify the method, we employed dual competitive padlock probe-mediated rolling circle amplification (DCPLP-RCA) to genotype the CYP2C9 gene. ResultsThe specificity was well improved through the competition and strand displacement of dual padlock probe, with an 83.26% reduction in nonspecific signal. By detecting synthetic RNA samples, the method demonstrated a dynamic detection range of 10 pmol/L-1 nmol/L. Furthermore, clinical samples were applied to the method to evaluate its performance, and the genotyping results were consistent with those obtained using the qPCR method. ConclusionThis study has successfully established a highly specific direct RNA SNP detection method, and provided a novel avenue for accurate identification of various types of RNAs.

Objective:To construct a set of rationality evaluation index system of medical service price items,so as to provide references and tools for improving the mechanism of finding abnormal medical service price items and the mechanism of monitoring and evaluating medical service price items.Methods:Literature Review,focus group discussion,Delphi method and Analytic Hierarchy Process were used to determine the evaluation indexes and their weights.Results:The rationality evaluation index system of medical service price items included 5 primary indexes,10 secondary indexes and 22 third-level indexes.According to the weight,primary indexes were cost compensation(0.253),clinical use(0.227),price comparison relationship(0.225),reform guidance(0.164)and technical evaluation(0.131).The positive coefficient of experts,1evel of authority and coordination of opinions were confirmed.The items whose specific single index score was zero or whose total score was below the threshold were considered as abnormal items.Conclusion:It constructed a set of rationality evaluation index system of medical service price items,which has high scientificity and reliability.Due to the prerequisites for obtaining the basic data required for the evaluation,it is recommended that a pilot project be carried out in the pilot areas of deepening the reform of medical service prices for the purpose of further verifying and optimizing the index system.

Objective:To construct a set of rationality evaluation index system of medical service price items,so as to provide references and tools for improving the mechanism of finding abnormal medical service price items and the mechanism of monitoring and evaluating medical service price items.Methods:Literature Review,focus group discussion,Delphi method and Analytic Hierarchy Process were used to determine the evaluation indexes and their weights.Results:The rationality evaluation index system of medical service price items included 5 primary indexes,10 secondary indexes and 22 third-level indexes.According to the weight,primary indexes were cost compensation(0.253),clinical use(0.227),price comparison relationship(0.225),reform guidance(0.164)and technical evaluation(0.131).The positive coefficient of experts,1evel of authority and coordination of opinions were confirmed.The items whose specific single index score was zero or whose total score was below the threshold were considered as abnormal items.Conclusion:It constructed a set of rationality evaluation index system of medical service price items,which has high scientificity and reliability.Due to the prerequisites for obtaining the basic data required for the evaluation,it is recommended that a pilot project be carried out in the pilot areas of deepening the reform of medical service prices for the purpose of further verifying and optimizing the index system.

Objective:To construct a set of rationality evaluation index system of medical service price items,so as to provide references and tools for improving the mechanism of finding abnormal medical service price items and the mechanism of monitoring and evaluating medical service price items.Methods:Literature Review,focus group discussion,Delphi method and Analytic Hierarchy Process were used to determine the evaluation indexes and their weights.Results:The rationality evaluation index system of medical service price items included 5 primary indexes,10 secondary indexes and 22 third-level indexes.According to the weight,primary indexes were cost compensation(0.253),clinical use(0.227),price comparison relationship(0.225),reform guidance(0.164)and technical evaluation(0.131).The positive coefficient of experts,1evel of authority and coordination of opinions were confirmed.The items whose specific single index score was zero or whose total score was below the threshold were considered as abnormal items.Conclusion:It constructed a set of rationality evaluation index system of medical service price items,which has high scientificity and reliability.Due to the prerequisites for obtaining the basic data required for the evaluation,it is recommended that a pilot project be carried out in the pilot areas of deepening the reform of medical service prices for the purpose of further verifying and optimizing the index system.

Objective:To construct a set of rationality evaluation index system of medical service price items,so as to provide references and tools for improving the mechanism of finding abnormal medical service price items and the mechanism of monitoring and evaluating medical service price items.Methods:Literature Review,focus group discussion,Delphi method and Analytic Hierarchy Process were used to determine the evaluation indexes and their weights.Results:The rationality evaluation index system of medical service price items included 5 primary indexes,10 secondary indexes and 22 third-level indexes.According to the weight,primary indexes were cost compensation(0.253),clinical use(0.227),price comparison relationship(0.225),reform guidance(0.164)and technical evaluation(0.131).The positive coefficient of experts,1evel of authority and coordination of opinions were confirmed.The items whose specific single index score was zero or whose total score was below the threshold were considered as abnormal items.Conclusion:It constructed a set of rationality evaluation index system of medical service price items,which has high scientificity and reliability.Due to the prerequisites for obtaining the basic data required for the evaluation,it is recommended that a pilot project be carried out in the pilot areas of deepening the reform of medical service prices for the purpose of further verifying and optimizing the index system.

Objective:To construct a set of rationality evaluation index system of medical service price items,so as to provide references and tools for improving the mechanism of finding abnormal medical service price items and the mechanism of monitoring and evaluating medical service price items.Methods:Literature Review,focus group discussion,Delphi method and Analytic Hierarchy Process were used to determine the evaluation indexes and their weights.Results:The rationality evaluation index system of medical service price items included 5 primary indexes,10 secondary indexes and 22 third-level indexes.According to the weight,primary indexes were cost compensation(0.253),clinical use(0.227),price comparison relationship(0.225),reform guidance(0.164)and technical evaluation(0.131).The positive coefficient of experts,1evel of authority and coordination of opinions were confirmed.The items whose specific single index score was zero or whose total score was below the threshold were considered as abnormal items.Conclusion:It constructed a set of rationality evaluation index system of medical service price items,which has high scientificity and reliability.Due to the prerequisites for obtaining the basic data required for the evaluation,it is recommended that a pilot project be carried out in the pilot areas of deepening the reform of medical service prices for the purpose of further verifying and optimizing the index system.

Objective:To construct a set of rationality evaluation index system of medical service price items,so as to provide references and tools for improving the mechanism of finding abnormal medical service price items and the mechanism of monitoring and evaluating medical service price items.Methods:Literature Review,focus group discussion,Delphi method and Analytic Hierarchy Process were used to determine the evaluation indexes and their weights.Results:The rationality evaluation index system of medical service price items included 5 primary indexes,10 secondary indexes and 22 third-level indexes.According to the weight,primary indexes were cost compensation(0.253),clinical use(0.227),price comparison relationship(0.225),reform guidance(0.164)and technical evaluation(0.131).The positive coefficient of experts,1evel of authority and coordination of opinions were confirmed.The items whose specific single index score was zero or whose total score was below the threshold were considered as abnormal items.Conclusion:It constructed a set of rationality evaluation index system of medical service price items,which has high scientificity and reliability.Due to the prerequisites for obtaining the basic data required for the evaluation,it is recommended that a pilot project be carried out in the pilot areas of deepening the reform of medical service prices for the purpose of further verifying and optimizing the index system.

Objective:To construct a set of rationality evaluation index system of medical service price items,so as to provide references and tools for improving the mechanism of finding abnormal medical service price items and the mechanism of monitoring and evaluating medical service price items.Methods:Literature Review,focus group discussion,Delphi method and Analytic Hierarchy Process were used to determine the evaluation indexes and their weights.Results:The rationality evaluation index system of medical service price items included 5 primary indexes,10 secondary indexes and 22 third-level indexes.According to the weight,primary indexes were cost compensation(0.253),clinical use(0.227),price comparison relationship(0.225),reform guidance(0.164)and technical evaluation(0.131).The positive coefficient of experts,1evel of authority and coordination of opinions were confirmed.The items whose specific single index score was zero or whose total score was below the threshold were considered as abnormal items.Conclusion:It constructed a set of rationality evaluation index system of medical service price items,which has high scientificity and reliability.Due to the prerequisites for obtaining the basic data required for the evaluation,it is recommended that a pilot project be carried out in the pilot areas of deepening the reform of medical service prices for the purpose of further verifying and optimizing the index system.

Magnetic separation(MS)is frequently used to enrich specific targets from biological sam-ples,which is commonly performed using antibodies coupled immunomagnetic beads(IMBs).However,due to the high cost of antibodies and difficulties in the process of releasing captured targets,IMBs have limitations for large-scale enrichment of targeted bioproducts.The specificities and affinities of aptamers towards their targets are in line with antibodies,but with characteristic of significantly lower costs of prep-aration,simpler structures,and higher chemical stability.In this study,taking advantages of the distinct adsorption features of graphene for single-stranded and double-stranded nucleic acids,we designed a novo system as bioseparation method that could display aptamers on graphene surfaces to enrich targets and then manipulate their release.This system mainly includes two units.Take CD63 aptamer as an exam-ple:double-stranded oligonucleotides scaffold attached to the graphene surface for CD63 aptamer exhibi-tion;and a single-stranded oligonucleotide complementary to the scaffold bipod sequence to desorb the captured targets from graphene.In this study,firstly,using graphene oxide(GO)paper as a graphene surface,we fluorescently labeled scaffolds or CD63 proteins and compared the fluorescence intensity difference on the GO paper before and after scaffold or CD63 protein release.Subsequently,a novo mate-rial,amino-modified graphene-shelled iron-nitrogen magnetic beads were introduced to carry the scaffold,and used to capture CD63 proteins from cell lysates.The results indicate that this scaffold can display the aptamer on the graphene surface for CD63 protein capture and controlled release.Using selected modified graphene magnetic beads carried with the scaffold,we achieved the capture of CD63 proteins from cell ly-sates.This system is expected to enrich various proteins such as CD63,or capture exosomes by hooking membrane proteins.