1.Construction of recombinant plasmid expressing S1 gene of new type of reovirus
Bing-Ke BAI ; Wei-Zhi HUANG ; Sheng-Dong LUO ; Yan HU ; Rong GAO ; Zhi-Jie WANG ; Qong HUANG ; Hao-Dong LIU ; Pan-Yong MAO
Chinese Journal of Experimental and Clinical Virology 2011;25(5):361-363
Objective To construct the recombinant plasmid containing S1 gene of new type of reovirus,and to study the expression of protein σ1 in Vero cells.Methods The recombinant plasmid,named pC-S,was constructed by cloning S1 gene into vector pCAGGS/MCS.Then Vero cells were transfected with pC-S and collected at 24,48,72 h post transfection followed by SDS-PAGE and Western-Blot assay.Results Results Both SDS-PAGE and Western-Blot assay indicated that σ1 protein could be expressed well and the highest expression level was 72 h post transfection.Conclusions σ1 protein could be expressed well in Vero cells by transfected with recombinant plasmid containing S1 gene,and could give some implications for subsequent research on virus-host interactions.
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