Nonaqueous capillary electrophoresis is used for the determination of the contents of diosgenin and ruscogenin in Radix Ophiopogonis. The operating buffer was composed of 20 mmol x L(-1) Na2B4O7-HCl (pH 7.61) in 70% methanol. The applied voltage was 25 kV and detection potential was at +0.70 V. With these conditions, the components were successfully separated. The content of diosgenin in Radix Ophiopogonis was 0.018 mg x g(-1) and ruscogenin was 0.008 mg x g(-1). The average recoveries of diosgenin and ruscogenin were 102% and 99.2%, respectively. A new method of the quality control of diosgenin and ruscogenin in Radix Ophiopogonis is provided.

AIM To determine the contents of the two free-and conjugated quercetin and genistein in Huaijiao Pills (Fructus Sohorae,Radix Sanguisorbae,Radix Scutellariae,etc.).by RP-HPLC. METHODS HPLC was employed with a column of Diamonsil ODS-C_ 18 (4.6 mm?150 mm,5.0 ?m) and a mobile phase of MeOH-H_2O-HAc(2∶ 3∶ 0.25),the detection wavelength being at 254 nm and the column temperature being at 30?C . RESULTS Nice linear correlation existed, when quercetin was within 0.204 8-1.229 ?g and genistein within 0.532 5-3.192 ?g.The correlation coefficient of each group was 0.999 1 and 0.999 8 respectively.The average recoveries of quercetin and genistein were 99.0 % and 99.1 % ,respectively.The RSDs were 1.02 % and 0.40 % ( n =5),respectively. CONCLUSION With this method,quercetin and genistein in Huaijiao Pills could be determined simultaneously and applicable to the quality control of Huaijiao Pill.