Endoscopic techniques has been widely used in radical surgical resection of various tumors， but there are still controversies over the application of laparoscopy in radical treatment of intrahepatic cholangiocarcinoma. In recent years， major medical centers have made many attempts to solve this problem. This article reviews the feasibility and safety of laparoscopy in the radical treatment of intrahepatic cholangiocarcinoma in recent years， in order to provide a reference for clinical practice.

The superior cervical ganglia (SCG) were dissected from neonatal rats.Dissoci-ated cell cultures were grown in Eagle's MEM supplemented with 20% calf serumwhich contain nerve growth factor (NGF).The cultures were divided into two groups:laser irradiation group and control.The former was exposed to lower dose of Helium-Neon laser (power:4 milliwatt)5 minutes every two hours.Two groups cultured for 20,22,24 and 28 hoursrespectively.Then the length of neurites of neurons were measured.At 22 hours,RNA and DNA synthesis of neurons labeled by ~3H-uridine and ~3H-thymidine wereobserved.The results showed that lower dose of Helium-Neon laser irradiation not onlypromotes growth of neurite but also enhances RNA synthesis of neurons in culture.However DNA synthesis in neurons does not promote in this experiment.