1. Immunomodulatory activity of polysaccharides from Brassica rapa by activating Akt/NF-κB signaling
Weiwei GUO ; Yu DU ; Junting GUO ; Liping BAI ; Qianxiao ZHANG ; Yu DU ; Junting GUO ; Tingting ZHAO ; Liping BAI ; Xiqiang AN ; Qianxiao ZHANG
Chinese Herbal Medicines 2022;14(1):90-96
Objective: To investigate the immunomodulatory activity of polysaccharides from the roots of Brassica rapa. Methods: The crude polysaccharide from roots of B. rapa (BRP) was extracted and purified to further investigate the active fraction of BRT for inducing macrophage phagocytosis. Results: Effects on RAW264.7 cells demonstrated that BRP behaved better phagocytic capacity and had potent immunomodulatory activity, including increasing production of nitric oxide (NO), tumor necrosis factor α (TNFα) and upregulating mRNA levels of inducible NO synthase (iNOS) and TNFα. Furthermore, modulation of macrophage by BRP was indicated to be mediated via the activation of Akt and nuclear factor-kappa B (NF-κB). Conclusion: The beneficial effects of BRP could be used as an immunotherapeutic adjuvant in treatment of inflammatory diseases.
Result Analysis
Print
Save
E-mail