Objective    To investigate the feasibility and safety of DynaCT microwave ablation (MWA) guided by 3D iGuide puncture technology for lung cancer. Methods    The clinical data of 19 patients with primary or metastatic lung cancer who underwent DynaCT MWA from June 2019 to December 2020 in our hospital were retrospectively analyzed, including 15 males and 4 females with an average age of 64.9±11.7 years. The technical success rates, adverse reactions and complications, postoperative hospital stay, and local therapeutic efficacy were recorded. Results    Technical success rate was 100.0%. The mean time required to target and place the needle was 15.7±3.7 min and the mean ablation time was 5.7±1.6 min. Thirteen patients underwent biopsy synchronously before the ablation, and 10 (76.9%) patients had positive pathological results. The main adverse reactions were pain (7/19, 36.8%), post-ablation syndrome (4/19, 21.1%) and cough (2/19, 10.5%). The minor complications were pneumothorax (6/19, 31.6%), hemorrhage (5/19, 26.3%), pleural effusion (2/19, 10.5%) and cavity (1/19, 5.3%). Three patients had moderate pneumothorax and received closed thoracic drainage. The median hospitalization time after ablation was 2.0 (2.0, 3.0) d, and no patient died during the perioperative period. The initial complete ablation rate was 89.5% (17 patients) and the incomplete ablation rate was 10.5% (2 patients) at 1-month follow-up, and no local progression was observed. Conclusion    DynaCT MWA of lung cancer under the guidance of 3D iGuide system is safe and feasible with a high short-term local control rate, but the long-term efficacy remains to be further observed.

Objective: To investigate the effects of retinoid-related orphan receptor γ (RORγ) gene on proliferation and metastasis of human colon cancer cells． Methods: RORγ knockdown cell lines were constructed and the knockdown efficiency was detected by RT-qPCR and Western blot assays ; MTT，colony formation，Transwell and wound healing assays were used to detect cell proliferation and metastasis ; the expression of epithelial-mesenchymal transition (EMT) related proteins was detected by Western blot．The relationship between RORγ gene expression and immune cell infiltration in tumor microenvironment was analyzed using TIMER 2. 0 database. Results : The knockdown of RORγ enhanced the viability (F = 157. 40，P＜0. 01) ，clonogenesis (F = 61. 35，P＜0. 01) ，migration (F = 13. 00，P＜0. 01) ，invasion (F = 21. 26，P＜0. 01) and wound healing ability (F = 877. 2，P＜0. 01) of colon cancer cells，inhibited the expression of E-Cadherin，and promoted the expression of vimentin and N-Cadherin．TIMER 2. 0 database analysis showed that RORγ expression in colon adenocarcinoma ( COAD) tissues was associated with multiple immune cell infiltrates． Conclusion Downregulation of RORγ expression promoted the proliferation and metastasis of colon cancer cells.

Objective To explore the pathogen spectrum of hand, foot, and mouth disease (HFMD) in Deyang, and the serological epidemiological characteristics of enterovirus 71 (EV71). Methods The general clinical data and etiological data of 287 patients with HFMD in Deyang from June 2019 to June 2021 were statistically analyzed. Blood samples were collected to detect serum EV71 neutralizing antibodies by neutralization test. Results Among the 287 patients with HFMD, most of the critically ill patients were between 1 and 6 years old. There was a statistically significant difference in clinical classification between different age groups (P < 0.05). A total of 254 (88.50%) EV-positive cases were detected, including 131 (45.64%) positive for EV71, 98 (34.15%) positive for CVA16, and 25 (8.71%) positive for other EVs. The EV71 positive rate was higher in critically ill patients (P < 0.05). The positive rate of EV71 antibody increased with age within 15 years old, with statistically significant differences among different age groups (P < 0.05). Among the 287 serum samples, there were 156 (54.36%) samples with neutralizing antibody titer <1:8, 86 (29.97%) with titer between 1:8 and 1:32, and 45 (15.68%) with titer >1:32. Patients with EV71 neutralizing antibody titer>1:32 were over 15 years old. There were statistically significant differences in EV71 neutralizing antibody titer between different age groups (P<0.05). Conclusion The positive rate of EV is different in patients with different clinical types. EV71 and CVA16 are main pathogens of HFMD in Deyang. HFMD patients under 6 years old have lower EV71 antibody level. It is recommended to strengthen EV71 vaccination for children under 6 years old.

Objective To investigate the mineral contents of colostrum and peripheral blood and their correlation in pregnant women in Beijing. Methods A total of 80 pregnant women who gave birth in the Affiliated Fuxing Hospital of Beijing Capital Medical University in May 2019 were selected as research subjects, and their colostrum and peripheral blood samples were collected. The contents of 46 minerals in the colostrum and peripheral blood samples were determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and Atomic fluorescence spectrum (AFS). The correlation between the contents of minerals in the colostrum and peripheral blood was analyzed by SPSS 21.0. Results Among the 46 minerals detected, the minerals with higher contents in the peripheral blood were sodium, potassium, iron, magnesium, calcium, zinc, rubidium, copper, aluminum and selenium. The minerals with higher colostrum contents included potassium, sodium, calcium, magnesium, zinc, iron, rubidium, copper, strontium and aluminum. The contents of calcium, strontium, cesium, molybdenum, cobalt, uranium and thorium in the colostrum were significantly higher than those in the peripheral blood (P<0.05). There were significant correlations in the contents of sodium, rubidium, selenium, strontium, cesium, arsenic, mercury and cadmium between the colostrum and peripheral blood (P<0.05). In addition, there were significant correlations between some minerals in the colostrum, for example potassium vs manganese (r=-0.236, P=0.043), rubidium (r=0.816, P<0.001) and magnesium (r=0.400, P<0.001); sodium vs selenium (r =0.509, P<0.001), cerium (r=0.353, P=0.002), praseodymium (r =0.337, P=0.003) and thulium (r=0.331, P=0.004); calcium vs iron (r=0.251, P=0.031) and strontium (r =0.365, P=0.001); magnesium vs cesium (r=0.314, P=0.006) and copper (r =0.395, P=0.001); zinc vs selenium (r=0.310, P=0.007), cerium (r=-0.229, P=0.050), gadolinium (r=-0.372, P=0.001) and yttrium (r=-0.380, P=0.001); rubidium vs titanium (r=-0.413, P<0.001); copper vs cesium (r=0.275, P=0.018); strontium vs uranium (r=0.439, P<0.001); cadmium vs molybdenum (r=0.379, P =0.001). Conclusion The correlations of the mineral contents between the colostrum and peripheral blood suggested that some mineral elements could be accumulated from blood to breast milk through various transport mechanisms. The correlations between the mineral elements in the colostrum showed that there were some interactions between these elements, indicating that maintaining the dynamic balance of mineral elements in human milk is very important for the health of their offspring.

Objective    To evaluate the safety and efficacy of microwave ablation (MWA) in the treatment of lung tumors. Methods    The clinical data of 31 patients with lung neoplasms treated with MWA from January 2019 to August 2020 in a single center were retrospectively analyzed. There were 17 males and 14 females at an age of 63.4±10.4 years. The characteristics of the lesions, technical success rate, technical efficiency, local progression rate, adverse reactions and complications were recorded in detail. Results    There were 39 target lesions with an average diameter of 20.2±10.6 mm. A total of 36 MWA procedures were completed. The initial technical success rate was 84.6% (33/39), and the technical efficiency was 92.3% (36/39). The median postprocedure hospital stay was 2.0 (2.0, 3.0) d. A total of 12.9% (4/31) of the patients had local progression, and the local control rate was 87.1%. The main adverse reactions were pain (12/36, 33.3%), cough (6/36, 16.7%), post-ablation syndrome (6/36, 16.7%) and pleural effusion (3/36, 8.3%). The main complications were pneumothorax (11/36, 30.6%), hemorrhage (8/36, 22.2%), cavitation (2/36, 5.6%) and pulmonary infection (1/36, 2.8%). The median follow-up time was 13.0 (8.0, 18.0) months. No patient died during the follow-up. Conclusion    MWA is safe and effective in the treatment of lung tumors with controllable complications. Successive researches with large sample, and medium and long-term follow-ups are needed to explore the significance of combined therapies.