Objective: To explore the effects of Cldn14 gene knockout on renal metabolism and stone formation in rats，so as to provide reference for research in the field of urinary calium metabolism and stone formation. Methods: Cldn14 gene knockout homozygous rats and wild-type rats of the same age were randomly divided into 4 groups：wild-type control (WC) group，wild-type ethylene glycol (WE) group，gene knockout control (KC) group and gene knockout ethylene glycol (KE) group，with 10 rats in each group.The WE and KE groups were induced with ethylene glycol + ammonium chloride to form kidney stones，while the WC and KC groups received normal saline gavage.After 4 weeks of standard maintenance feeding，the urine samples were collected to detect the venous blood.The kidneys were collected for HE，Pizzolatto's staining and transmission electron microscopy.The protein in renal tissues was extracted to detect the expressions of Claudin16 and Claudin19. Results: Crystal deposition was observed in the renal tubular lumen of the WE and the KE groups，and more crystals were detected in the KE group.The WE group had a large number of intracytoplasmic black crystalline inclusions observed in renal tubular epithelial cells under transmission electron microscope，followed by the KE and KC groups.Compared with WC and WE groups，KC and KE groups had significantly decreased serum calcium and magnesium levels but significantly increased urinary calcium level.In addition，the urinary calcium level was higher in the WE group than in the WC group and higher in the KE group than in the KC group.The KE group had lower level of Claudin16，but there was no significant difference in the level of Claudin19 among the 4 groups(P>0.05). Conclusion: Knockout of Cldn14 gene alone cannot effectively reduce urinary calcium excretion or reduce the risk of stone formation in rats，which may be related to the decrease of Claudin16 level.

Radiotherapy can cause functional and morphological changes in the brain tissues of patients with primary or metastatic malignant brain tumors, leading to radiation-induced brain injury. However, the pathogenesis of radiation-induced brain injury has not yet been unanimously determined, and its research advances and treatment protocols are yet to be elucidated and improved. In this study, we explore the pathogenesis of radiation-induced brain injury from the perspective of vascular injury, inflammatory reactions, neuronal dysfunction, glial cell injury, and gut microbiota and reviewed the advances in research on its treatment and prevention. The purpose is to provide a reference and theoretical basis for the research and clinical diagnosis and treatment of radiation-induced brain injury.

Objective To compare the short-term outcomes of thulium laser enucleation of the prostate(ThuLEP)and plasmaknife enucleation of the prostate(PKEP)in the treatment of large volume(≥80 mL)benign prostatic hyperplasia(BPH),so as to provide reference for clinical practice.Methods Clinical data of 100 patients with large volume BPH admitted to the Shandong Provincial Third Hospital affiliated with Shandong University during Jun.2021 and Jun.2023 were retrospectively analyzed.Based on the surgical methods,patients were divided into the ThuLEP group(n=50)and PKEP group(n=50).The following parameters were compared between the two groups,including preoperative maximum flow rate(Qmax),international prostatic symptom score(IPSS),quality of life score(QoL),operation time,intraoperative blood loss,weight of enucleated prostate tissue,postoperative bladder irrigation time,catheterization time,hospital stay,complication rate,and postoperative Qmax,IPSS,and QoL at 3 months.Results The operation time[(79.04±9.42)min vs.(80.10±8.94)min],intraoperative blood loss[(42.86±7.84)mL vs.(43.32±7.36)mL],and postoperative continuous bladder irrigation time[(19.88±4.13)h vs.(20.10±4.04)h]were not statistically different between the ThuLEP and PKEP groups(P＞0.05).The weight of enucleated tissue during ThuLEP was higher than that during PKEP[(69.44±4.90)g vs.(65.06±5.25)g],postoperative catheterization time was shorter[(27.20±4.07)h vs.(29.02±4.86)h],and hospital stay was shorter[(3.30±0.66)d vs.(4.05±0.91)d],with statistically significant differences(P＜0.05).Three months after operation,the Qmax,IPSS,and QoL score in both groups were significantly improved(P＜0.05),but there were no statistically significant differences between the two groups(P＞0.05).There was no statistically significant difference in the complication rate between the ThuLEP and PKEP groups(2％vs.4％,P＞0.05).Conclusion Both ThuLEP and PKEP have good short-term efficacy and safety in the treatment of large volume BPH,but ThuLEP is superior to PKEP in terms of reducing enucleated prostate weight,and shortening postoperative catheterization time and hospital stay.

Objective To explore the impact of post-intensive care syndrome (PICS) of ICU survivors on burden of primary caregivers. Methods This study included ICU survivors and primary caregivers who meeting the conditions between 2016.4.1 and 2017.6.30. Mini-mental State Examination, The Hospital Anxiety and Depression Scale, Post-traumatic Stress Disorder Self-rating Scale and the Barthel Index Rating Scale were used to assess PICS in ICU survivors at 3 months after discharge from ICU. At the same time, the Chinese Zarit Burden Interview was used to assess the primary caregivers ' burden. Univariate and multivariate logistic regression were used to analyze the relationship between PICS and primary caregiver burden. Results PICS occurred in 127 patients. 228 (58.9%) caregivers had mild or no burden, 102 (26.4% ) had moderate burden and 57 (14.7% ) had severe burden. Logistic multiple regression analysis showed that the patient's PICS was an independent risk factor for the caregiver's burden [ OR=1.547(1.143-6.189), P=0.018]. Conclusions PICS of ICU survivors can increase the burden of primary caregivers. Necessary PICS education for patients and caregivers may have a certain significance to reduce the level of burden.

Objective To detect osteopontin (OPN) expression level of cervical cancer patients before and after chemotherapy , and explore the clinical value in the evaluation of chemotherapy efficacy .Methods 90 cases of cervical squamous cell carcinoma were treated with TP regimen ,immunohistochemical method was used to detect OPN expression before and after chemotherapy ,and studied the significance of OPN evaluation on curative effect in cancer chemotherapy .Results Before chemotherapy the positive ex-pression rate of OPN of tissue was 85 .56% ,and that after chemotherapy was 62 .22% ,the positive rate of OPN expression has sig-nificant difference before and after chemotherapy conclusion(χ2 =14 .118 ,P<0 .05) .The positive expression rate of OPN of effec-tive chemotherapy group before chemotherapy was 78 .69% ,and that after chemotherapy was 47 .54% ,there were significant differ-ences(χ2 =20 .840 ,P<0 .05) .Before chemotherapy ,the positive expression rate of OPN in ineffective chemotherapy was 100% ,af-ter chemotherapy ,it was 96 .56% ,there were no significant difference(χ2 =3 .046 ,P>0 .05) .Patients with OPN negative expres-sion rate of CR was 100% (13/13) ,OPN patients with positive expression rate of CR was 45 .46% (35/77) ,compared two groups with statistical difference (P<0 .05) .Conclusion The expression of OPN in cervical cancer tissue in the tumor is related with chemotherapy sensitivity and effect of chemotherapy .

To clarify the function of miR-103 in the differentiation of porcine preadipocyte, we carried out real-time PCR to detect the expression pattern of miR-103 during adipogenesis, and clarified its expression tendency through cell differentiation. Then we used adenovirus that overexpressed miR-103 to infect porcine preadipocyte. Subsequently, mRNA and protein expression of adipogenesis marker--PPARgamma and aP2 was analyzed by real-time PCR and Western blotting. At last, Oil-Red O staining was used to detect lipids accumulation in the 8th day after adipogenic inducement. The expression of miR-103 increased during adipocyte differentiation; compared with the control, the preadipocyte infected by pAd-miR-103 had an elevated expression level of adipocyte marker gene PPARgamma, aP2, and obvious lipid droplet was seen in the 8th day after adipogenic inducement. These results showed that miR-103 can enhance adipogenesis in primary cultured porcine adipocytes.
Adenoviridae ; genetics ; metabolism ; Adipocytes ; cytology ; metabolism ; Adipogenesis ; genetics ; Animals ; Base Sequence ; Cell Differentiation ; MicroRNAs ; genetics ; metabolism ; Molecular Sequence Data ; PPAR gamma ; genetics ; metabolism ; Primary Cell Culture ; RNA, Messenger ; genetics ; metabolism ; Swine ; Transfection