1. Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool: Validation method using strains from Colombia classified according to their discrete typing unit
Francisco OLMO ; Patricia PALMA ; Manuel SÁNCHEZ-MORENO ; Clotilde MARÍN ; Javier ESCOBEDO-ORTEG ; Ana MEJÍA-JARAMILLO ; Omar TRIANA
Asian Pacific Journal of Tropical Medicine 2014;7(11):854-859
Objective: To classify 21 new isolates of Trypanosoma cruzi (. T. cruzi) according to the Discrete Typing Unit (DTU) which they belong to, as well as tune up a new pair of primers designed to detect the parasite in biological samples. Methods: Strains were isolated, DNA extracted, and classified by using three Polymerase Chain Reactions (PCR). Subsequently this DNA was used along with other isolates of various biological samples, for a new PCR using primers designed. Finally, the amplified fragments were sequenced. Results: It was observed the predominance of DTU I in Colombia, as well as the specificity of our primers for detection of T. cruzi, while no band was obtained when other species were used. Conclusions: This work reveals the genetic variability of 21 new isolates of T. cruzi in Colombia.Our primers confirmed their specificity for detecting the presence of T. cruzi.
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