The acquired immunodeficiency syndrome patients with compromised immunity are prone to hemophagocytic syndrome secondary to opportunistic infections.This paper reports a rare case of hemophagocytic syndrome secondary to human parvovirus B19 infection in an acquired immunodeficiency syndrome patient,and analyzes the clinical characteristics,aiming to improve the diagnosis and treatment of the disease and prevent missed diagnosis and misdiagnosis.
Humans ; Lymphohistiocytosis, Hemophagocytic/drug therapy* ; Erythema Infectiosum/complications* ; Acquired Immunodeficiency Syndrome/complications* ; Parvoviridae Infections/diagnosis* ; Parvovirus B19, Human

Human parvovirus B19 (B19 virus) is one of the two parvoviruses that cause human diseases. As an important pathogen to humans, it causes infectious erythema in children, acute aplastic anemia, fetal edema and death. In this review, we focus on the recent advances in the molecular virology of B19V, such as viral genotypes, viral receptor, genomic features and viral replication, viral transcription and post-transcription regulation, viral nonstructural and structural protein features and functions, viral diagnosis and antiviral agents, to provide reference for further study of B19 pathogenesis mechanisms, treatment and diagnostic strategies.
Antiviral Agents ; DNA, Viral ; genetics ; Erythema Infectiosum ; diagnosis ; virology ; Genotype ; Humans ; Parvovirus B19, Human ; genetics ; Virology ; trends ; Virus Replication

Calf diarrhea caused by infectious agents is associated with economic losses in the cattle industry. The purpose of this study was to identify the causative agents and epidemiological characteristics of diarrhea in Korean native calves (KNC). In total, 207 diarrheal KNC aged less than 7 months were investigated. Fecal samples collected from the rectum were examined for causative agents using polymerase chain reaction (PCR) or real-time PCR and the number of oocysts were counted. Fourteen causative agents were detected from 164 of the 207 diarrheal KNC. Rotavirus was the most common agent (34.8%), followed by Eimeria spp. (31.7%), Escherichia coli (22.0%), Giardia spp. (14.0%), Clostridium difficile (9.8%), bovine viral diarrhea virus (8.5%), coronavirus (7.9%), Cryptosporidium spp. (7.3%), torovirus (6.7%), parvovirus (5.5%), norovirus (4.9%), kobuvirus (1.8%), adenovirus (1.2%), and Salmonella spp. (0.6%). About 95 (57.9%) of 164 calves were infected with a single causative agent and 42.1% were infected by multiple agents. No significant difference was observed in mortality between calves infected with a single agent and multiple agents. The occurrence of diarrhea caused by rotavirus, Eimeria spp., kobuvirus, and Giardia spp. was significantly different based on onset age, and the prevalence of diarrhea caused by rotavirus or C. difficile was significantly different between seasons. This study help the understanding of KNC diarrhea for the development of an effective strategy for disease prevention and control, especially in Eastern provinces of South Korea.
Adenoviridae ; Age of Onset ; Animals ; Cattle ; Clostridium difficile ; Coronavirus ; Cryptosporidium ; Diarrhea ; Eimeria ; Epidemiology ; Escherichia coli ; Giardia ; Kobuvirus ; Korea ; Mortality ; Norovirus ; Oocysts ; Parvovirus ; Polymerase Chain Reaction ; Prevalence ; Real-Time Polymerase Chain Reaction ; Rectum ; Rotavirus ; Salmonella ; Seasons ; Torovirus

Kikuchi-Fujimoto disease (KFD) is a self-limiting disease characterized by subacute necrotizing lymphadenitis. This benign disease is frequently associated with prolonged fever and mostly occurs in young Asian women. KFD is generally diagnosed using a biopsy of affected lymph nodes and spontaneously resolves in several months. Although the causative agent is believed to be infectious, the etiology remains unknown. Some cases of KFD are associated with viral infections, including Epstein-Barr virus, human herpes virus 6, and parvovirus B19 infection. Herein, we report a case of KFD associated with Mycoplasma pneumoniae infection.
Asian Continental Ancestry Group ; Biopsy ; Female ; Fever ; Herpesvirus 4, Human ; Histiocytic Necrotizing Lymphadenitis ; Humans ; Lymph Nodes ; Lymphadenitis ; Mycoplasma pneumoniae ; Mycoplasma ; Parvovirus ; Pneumonia, Mycoplasma

Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 10⁴/mL and 9.85 × 10³/mL for samples with a 2-fold serial dilution from 1.25 × 10⁶ TCID₅₀ (TCID₅₀, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.
Animals ; Coronavirus ; Coronavirus, Canine ; Diagnostic Tests, Routine ; Distemper ; Dogs ; Enteritis ; Escherichia coli ; Immunoassay ; Limit of Detection ; Parvovirus, Canine ; Polymerase Chain Reaction ; Reagent Kits, Diagnostic ; RNA Viruses ; Sensitivity and Specificity

Porcine parvovirus 7 (PPV7) was first detected in Korean pig farms in 2017. The detection rate of PPV7 DNA was 24.0% (30/125) in aborted pig fetuses and 74.9% (262/350) in finishing pigs, suggesting that PPV7 has circulated among Korean domestic pig farms. Phylogenetic analysis based on capsid protein amino acid sequences demonstrated that the nine isolated Korean strains (PPV-KA1-3 and PPV-KF1-6) were closely related to the previously reported USA and Chinese PPV7 strains. In addition, the Korean strains exhibit genetic diversity with both insertion and deletion mutations. This study contributes to the understanding of the molecular epidemiology of PPV7 in Korea.
Aborted Fetus ; Agriculture ; Amino Acid Sequence ; Asian Continental Ancestry Group ; Capsid Proteins ; DNA ; Fetus ; Genetic Variation ; Humans ; Korea ; Molecular Epidemiology ; Parvovirus, Porcine ; Sequence Deletion ; Sus scrofa ; Swine

Neuralgic amyotrophy (NA) is a peripheral neuropathy, primarily involving the brachial plexus. There is a relation between antecedent infection and NA. A few cases of NA after infections such as Epstein-Barr virus, herpes zoster virus, parvovirus, human immunodeficiency virus, Borrelia, and other infections have been reported. This case report describes a 26-year-old man with motor impairment after neuropathic pain with preceding mild flu-like symptoms whose laboratory studies revealed evidence of cytomegalovirus (CMV) infection. He was diagnosed with NA associated with CMV infection. In conclusion, CMV is a rare but possible pathogen of NA.
Adult ; Borrelia ; Brachial Plexus ; Brachial Plexus Neuritis ; Cytomegalovirus Infections ; Cytomegalovirus ; Herpesvirus 3, Human ; Herpesvirus 4, Human ; HIV ; Humans ; Immunocompetence ; Neuralgia ; Parvovirus ; Peripheral Nervous System Diseases

Wild raccoon dogs (Nyctereutes procyonoides koreensis) may play a role transmitting several pathogens to humans and pet animals. Information concerning the incidence of rabies, canine distemper virus (CDV), canine parvovirus (CPV), canine adenovirus type 2 (CAdV-2), canine parainfluenza virus type 5 (CPIV-5), and canine herpesvirus (CHV) is needed in wild raccoon dogs. In total, 62 brain samples of raccoon dogs were examined for rabies virus (RABV) and CDV, and 49 lung samples were screened for CDV, CAdV-2, CPIV-5, and CHV. No RABV, CAdV-2, CPIV-5, or CHV was identified, but nine CDV antigens (8.1%, 9/111) were detected. Moreover, 174 serum samples from wild raccoon dogs were screened for antibodies against the five major viral pathogens. The overall serosurveillance against CDV, CPV, CAdV-2, CPIV-5, and CHV in wild raccoon dogs was 60.3%, 52.9%, 59.8%, 23.6%, and 10.3%, respectively. Comparisons of the sero-surveillance of the five pathogens showed that raccoon dogs of Gyeonggi province have slightly higher sero-positive rates against CDV, CPV, and CHV than those of Gangwon province. These results indicate high incidences of CDV, CPV, and CAdV-2 in wild raccoon dogs of two Korean provinces and a latent risk of pathogen transmission to companion and domestic animals.
Adenoviruses, Canine ; Animals ; Animals, Domestic ; Antibodies ; Brain ; Disease Transmission, Infectious ; Distemper ; Distemper Virus, Canine ; Friends ; Gangwon-do ; Gyeonggi-do ; Humans ; Incidence ; Lung ; Paramyxoviridae Infections ; Parvovirus, Canine ; Rabies ; Rabies virus ; Raccoon Dogs* ; Raccoons*

BACKGROUND: Due to the tropism of human parvovirus B19 to erythroid progenitor cells, infection in patients with an underlying hemolytic disorder such as beta-thalassemia major leads to suppression of erythrocyte formation, referred to as transient aplasia crisis (TAC), which may be life-threatening. We investigated the prevalence of parvovirus B19 among patients with beta thalassemia major attending the Zafar Adult Thalassemia Clinic in Tehran, Iran. METHODS: This cross-sectional study was performed to determine the presence of parvovirus B19 DNA in blood samples and parvovirus B19 genotypes in plasma samples of patients with thalassemia major. The population consisted of 150 patients with beta-thalassemia major who attended the Zafar clinic in Tehran. Specimens were studied using a real-time polymerase chain reaction assay. RESULTS: The prevalence of parvovirus B19 in our study population was 4%. Of 150 patients with thalassemia, six (4%) were positive for B19 DNA. There was no significant correlation between blood transfusion frequency and B19 DNA positivity. Finally, phylogenetic analysis of human parvovirus B19 revealed genotype I in these six patients. CONCLUSION: In this study, acute B19 infections were detected in patients with beta thalassemia major. Screening of such high-risk groups can considerably reduce the incidence and prevalence of B19 infection; thus, screening is required for epidemiologic surveillance and disease-prevention measures.
Adult ; beta-Thalassemia* ; Blood Transfusion ; Cross-Sectional Studies ; DNA ; Epidemiological Monitoring ; Erythrocytes ; Erythroid Precursor Cells ; Genotype ; Humans* ; Incidence ; Iran* ; Mass Screening ; Parvovirus ; Parvovirus B19, Human* ; Plasma ; Prevalence ; Real-Time Polymerase Chain Reaction ; Thalassemia ; Tropism

Porcine parvovirus, Erysipelothrix (E.) rhusiopathiae, and Leptospira (L.) interrogans are considered major etiologic agents of reproductive failure in pigs, causing economic loss in the swine industry. In this study, the safety and immunogenicity of a new octavalent inactivated vaccine were evaluated. The vaccine contained inactivated porcine parvovirus, E. rhusiopathiae, and six L. interrogans serovars (Bratislava, Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, and Pomona). Safety test results showed no notable side effects or clinical signs after vaccination in mice, guinea pigs, and sows. In addition, we assessed immunogenicity of the vaccine in 25 sows under field conditions. The vaccinated group (n = 20) had a significantly higher antibody level than the non-vaccinated group (n = 5). Moreover, the stillbirth rate decreased in piglets born from vaccinated sows, resulting in an increased fertility rate. The results of this study demonstrate that the new octavalent inactivated vaccine can be applied safely and effectively to improve reproductive performance in sows.
Animals ; Birth Rate ; Erysipelas* ; Erysipelothrix ; Guinea Pigs ; Leptospira* ; Leptospirosis ; Mice ; Parvovirus, Porcine* ; Serogroup ; Stillbirth ; Swine ; Vaccination