The Shine Muscat is a table grape, popular in South Korea for its unique mango-flavor taste.Flyspeck is a disease that is characterized by small, black, and circular specks on the grape cuticle was first observed in several commercial orchards in Sangju, South Korea, in August 2019. Here we identified the causal agent of flyspeck based on an advanced diagnosis approach, comprised of both morphological and molecular analyses. Morphological characteristics of the cultures isolated from grape flyspeck were identical to the fungus Cladosporium perangustum. The concatenated sequences of ITS, ACT, and EF1-α were used for molecular phylogenetic analysis, BLAST searches along with Bayesian inference-based phylogeny, confirmed that the causal agent of grape flyspeck is C. perangustum. The cultured fungal isolates also produced flyspeck symptoms on healthy fruits in pathogenicity tests. To the best of my knowledge, this is the first documented evidence of any Cladosporium sp. producing flyspeck symptoms on any plant.

In May to July 2019, ovate-leaf atractylodes seedling and plant with Damping-off symptoms were observed in farmer field at Sangju and Mungyeong, Korea. Seven fungal isolates have been retrieved from diseased root tissue and identified as Rhizoctonia solani AG-5 based on morphological and molecular characteristics. To the best of our knowledge, this is the first report on damping-off of ovate-leaf atractylodes caused by R. solani AG-5 in South Korea.

The Shine Muscat is a table grape, popular in South Korea for its unique mango-flavor taste.Flyspeck is a disease that is characterized by small, black, and circular specks on the grape cuticle was first observed in several commercial orchards in Sangju, South Korea, in August 2019. Here we identified the causal agent of flyspeck based on an advanced diagnosis approach, comprised of both morphological and molecular analyses. Morphological characteristics of the cultures isolated from grape flyspeck were identical to the fungus Cladosporium perangustum. The concatenated sequences of ITS, ACT, and EF1-α were used for molecular phylogenetic analysis, BLAST searches along with Bayesian inference-based phylogeny, confirmed that the causal agent of grape flyspeck is C. perangustum. The cultured fungal isolates also produced flyspeck symptoms on healthy fruits in pathogenicity tests. To the best of my knowledge, this is the first documented evidence of any Cladosporium sp. producing flyspeck symptoms on any plant.

In May to July 2019, ovate-leaf atractylodes seedling and plant with Damping-off symptoms were observed in farmer field at Sangju and Mungyeong, Korea. Seven fungal isolates have been retrieved from diseased root tissue and identified as Rhizoctonia solani AG-5 based on morphological and molecular characteristics. To the best of our knowledge, this is the first report on damping-off of ovate-leaf atractylodes caused by R. solani AG-5 in South Korea.

Plum pocket caused by the dimorphic ascomycetous fungi, Taphrina spp., results in unsightly malformations and crop loss. In 2016, Japanese plums (Prunus salicina Lindl.) with plum pocket symptoms were found in Gimcheon. Three isolates were collected from symptomatic P. salicina fruits and identified as Taphrina deformans based on morphological characteristics and molecular sequence analysis of including internal transcribed space (ITS) and the mitochondrial small ribosomal subunit (SSU) regions of the three isolates. Pathogenicity test on plum fruits confirmed that, the present T. deformans isolates are causal agent of plum pocket. To the best of our knowledge, this is the first report of plum pocket caused by T. deformans in South Korea.

Anthracnose is one of the major problems for cultivating many crops, including vegetables,fruits, and trees. It is a continual threat for fruits grower worldwide. Colletotrichumfructicola was isolated from Shine Muscat berries showing typical anthracnose symptomin Korea. It was identified as C. fructicola based on morphology, pathological signs andconcatenated sequences of internal transcribed spacer region of rDNA, glyceraldehyde-3-phosphate dehydrogenase, b-tubulin-2, chitin synthase-1, calmodulin, and the Apn2-Mat1-2 intergenic spacer and partial mating type (Mat1-2) gene. To the best of our knowledge,this is the first report first report of anthracnose of Shine Muscat caused by C. fructicolain Korea.

Plum pocket caused by the dimorphic ascomycetous fungi, Taphrina spp., results in unsightly malformations and crop loss. In 2016, Japanese plums (Prunus salicina Lindl.) with plum pocket symptoms were found in Gimcheon. Three isolates were collected from symptomatic P. salicina fruits and identified as Taphrina deformans based on morphological characteristics and molecular sequence analysis of including internal transcribed space (ITS) and the mitochondrial small ribosomal subunit (SSU) regions of the three isolates. Pathogenicity test on plum fruits confirmed that, the present T. deformans isolates are causal agent of plum pocket. To the best of our knowledge, this is the first report of plum pocket caused by T. deformans in South Korea.

Anthracnose is one of the major problems for cultivating many crops, including vegetables,fruits, and trees. It is a continual threat for fruits grower worldwide. Colletotrichumfructicola was isolated from Shine Muscat berries showing typical anthracnose symptomin Korea. It was identified as C. fructicola based on morphology, pathological signs andconcatenated sequences of internal transcribed spacer region of rDNA, glyceraldehyde-3-phosphate dehydrogenase, b-tubulin-2, chitin synthase-1, calmodulin, and the Apn2-Mat1-2 intergenic spacer and partial mating type (Mat1-2) gene. To the best of our knowledge,this is the first report first report of anthracnose of Shine Muscat caused by C. fructicolain Korea.