Background: Cancer incidence and mortality are steadily increasing both globally and in Mongolia. As these rates rise, traditional Mongolian medicine has long utilized herbal formulas for the treatment of gastric and esophageal cancers and precancerous conditions. One such formulation—Hot Natured 3 Herbs (HN3H)—comprises three species from the Ranunculaceae family: Atragene sibirica L., Ranunculus repens L., and Pulsatilla bungeana L.. However, scientific validation of its anti-tumor effects is essential. This study aimed to investigate the effect of HN3H in a tumor-induced animal model. Aim: To identify the biologically active compounds of HN3H and evaluate their effect in an experimentally induced tumor model in animals. Materials and Methods: The three herbs comprising HN3H—Atragene sibirica L., Ranunculus repens L., and Pulsatilla bungeana L.—were collected during their flowering stage (May–June) in Khishig-Undur, Bulgan province, and dried according to official procedures. Extraction was carried out by maceration in 96% ethanol at a 1:10 ratio. The concentrated extract was suspended in water (1:1) and successively fractionated with dichloromethane, ethyl acetate, butanol, chloroform, and n-hexane. The study was approved by the Research Ethics Committee of the Mongolian National University of Medical Sciences (Protocol №2020/03-04). A colorectal cancer model was established by subcutaneous injection of MC-38 cells (Kerafast, USA) into C57BL/6 mice. Immunohistochemistry was performed using CK20, CDX2, Ki67, and p53 antibodies at 1:100 and 1:200 dilutions. Results: The ethanol extract of HN3H contained 2.98±0.04% total phenolics and 2.16±0.05% total flavonoids. Body weight and tumor volume were measured daily with three repetitions. All groups showed a time-dependent increase in body weight. Mice in groups 1A and 1B received ethanol extract at 50 and 100 mg/kg doses; groups 2A and 2B received dichloromethane extract at the same doses. The negative control group was administered 0.5 mg/kg PBS orally, while the positive control group received intraperitoneal injections of 5-fluorouracil (5FU) at 10 mg/kg twice a week. Tumor growth increased in a time-dependent manner across groups. Compared to the negative control, tumor volumes in four treatment groups showed statistically significant reduction (p˂0.05), while no significant difference was observed when compared to the positive control (p=0.08). Histological analysis revealed necrosis in all groups, with variation in extent. Conclusion The ethanol extract of HN3H exhibited moderate levels of phenolic compounds and a high concentration of flavonoids. HN3H extract inhibited tumor progression and activated lymphocyte-predominant inflammation in tumor tissues, indicating potential anti-tumor activity (p˂0.05).

Background: The high-performance liquid chromatography (HPLC) method was developed to select salidroside in tablet formulation dietary supplements, raw material containing other components. Further, the proposed method was validated for linearity, precision (system precision, method precision, intermediate or inter-day precision), and accuracy, stability in analytical solution, syst em suitability, and ruggedness. The developed method exhibited the best results in terms of the validation above parameters. The other components and additives did not interfere with their determinations. The method was found to be selective, simple, economical, accurate, reproducible, rapid, and reliable for routine estimation purposes of salidroside in golden root dry extract. The goal of this study was to develop the validation method of salidroside in the dietary supplement. Material and Methods: The Rhodiola rosae L. dry extract was supplied Arshin Co.ltd in People’s Republic of China. The standard salidroside was supplied from Sigma Aldrich Co Ltd. We used solvents for HPLC grade (methanol, acetonitrile). Chromatographic conditions: A gradient HPLC (Shimadzu CBM20AD) with serial dual plunger pump; analytical column: Shimadzu GIST С18 150 x 4.6 mm, particle size 5 μm; flow rate: 1 ml/min; column temperature: 400C, detection: UV 275 nm. Chromatographic procedure: 20 μl of the mixed standard preparation and assay (sample) preparation were separately injected into the chromatography, the chromatograms were recorded, and the responses for the major peaks were measured. The run time was approximately 15 minutes. Results The calibration curves for the salidroside were made by plotting the peak area versus the concentration for each analyte using regression analysis. Each calibration curve was obtained using six levels of concentrations in the range of 100-800 µg/mL. The linear correlation coefficient (r2=1) for all calibration curves was higher than 1 for all analytes. The LOD and LOQ for salidroside were golden root dry extract in 8.788 µg/mL and 26.61 µg/mL, respectively. Accuracy and precision were assessed by analyzing five samples independently prepared at low, middle, and high concentrations. The RSD values of repeatability and intermediate precision were below 1.12%, 1.19 and 1.79%. The accuracy remains between 91 to 109%. The resulting accuracy data were satisfactory for the quantitative analysis of salidroside in golden root dry extract. This article presents a simple, accurate, reproducible, and thoroughly validated HPLC-based method for qualitative and quantitative analysis of salidroside, as part of the quality assessment of golden root dry extract.

Introduction: The development of analytical methods for evaluation of quality standard parameters of drugs and other health prophylactic, diagnostic, and therapeutic products is essential from the beginning in the process to release to the market finished product. Validation of developed analytical methods is one crucial part of the analysis.
Quantitative determination methods of the total main active compounds of the tincture prepared from the root and the herb of Paeonia anomala L. were developed. In order to verify whether the analytical procedure is suitable for its intended purpose parameters – accuracy, reproducibility, intermediate precision, and linearity were validated. Materials and methods: The total phenolics in the tincture were determined by the reaction with Folin Chicolteau reagent and total monoterpene glycosides by the reaction with hydroxylamine in alkaline solution and ferric (III) chloride following spectrophotometric examination, respectively. Based on the achieved results of the developed quantitative methods which were validated according to the accepted and pursued technical documents in our country the Eurachem guide “The Fitness for Purpose of Analytical Methods” and Russian Pharmacopoeia 13 “Validation of analytical methodology”, where parameters as accuracy, precision – reproducibility, intermediate precision, and linearity are involved. Conclusions Statistical analysis of total phenolic compounds and total monoterpene glycosides in the Paeonia anomala tincture demonstrated that the bias calculated was for accuracy 0.674% and 0.703%, relative standard deviations were for reproducibility 0.896% and 0.798%, for inter- mediate precision 0.922% and 0.696%, respectively and the linearity – R²=0.9985 and R²=0.9997. All achieved results validated the appropriateness of the developed quantitative methods for the intended purpose.

Background: In 2011-2020, WHO approved the Global Strategy for Traditional Medicine. The strategy states that "The trend of using traditional medicine and alternative medicine as a source and resource for health care is expanding worldwide."
In this regard, it is important to develop a policy and methodology to develop a proper combination of traditional and modern medicine. Also, there are demands to study and solve the problems of ensuring the safe, effective, quality, availability, and appropriate use of medicines and treatments. Purpose: Finding traditional medicinal recipes containing the herb Veronica incana L from the ancient Mongolian medical books written by famous Mongolian scientists Jambalchoizhidanzanperenlei, Luvsandorj, and Jigmeddanzanjamts. Research materials and methods:
1. Research materials: Jambalchoijidanzanperenlei. gso rig bstan bcos mtha' dag gi snying po rnams phyogs gcig tu bsdus pa man ngag rin chen 'byung gnas bzhugs so. Wooden printing block with Tibetan script. Luvsandorj. sman sbyar tshad kyi skor rnams che long tsam phyogs gcig tu bkod ba kun phan bdud rtsi'i snying bo zhes bya ba bzhugs so). Wooden printing block with Tibetan script. Jigmeddanzanjamts. rgya badmkhas grub du ma'i gzhung lugs dam pa gong ma rnams kyi man ngag zhal shes lag len myong grub gnad don gnyen po ngo sprod mdzub tshugs dmar khrid kyi bkod pa mthong ba don gsal dga' byed snying po'i rnam gsal bzhugs so. Wooden printing block with Tibetan script.
2. Research methods: Methods of textual analysis for ancient books and checklist methods were used in the study. Conclusion Medicinal recipes containing the herb Veronica Incana L were sampled from the ancient medical books surveyed. There are 6 recipes in the book of Jambalchojidanzanperenlei, 3 recipes in the book of Luvsandorj, and 2 in the book of Jigmeddanzanjamts, a total of 11 ingredients. In general, these ancient medical books had a significant impact on the development of traditional Mongolian medicinal prescriptions. Therefore, even today, these works are still important in the theory of pharmaceutical composition and the practice of drug therapy.

Background: Medicinal plant research has been successfully carried out in the field of pharmacy and pharmacology in Mongolia, and dozens of research projects are still being carried out However, there are still fewer studied medicinal plants of practical importance growing in Mongolia. Therefore, there is a need to register and study the ancient medicinal books written by ancient Mongolian doctors about the relatively lilUe-studied Veronica Incana L plant. In addition, taking into account that scientific research on Veronica Incana L. which grows in Mongolia, has not been done in the field of medicine, the topic was selected for research in this field. Purpose: Comparing the information of the ancient medical books written by Mongolian doctors and scientists, who wrote about Veronica Incana L, to clarify the taste, power, and quality Research materials and methods:
1. Research materials: Sumbe khamba Ishoaljir. gso dpyad bdud rtsi 'i chu rgyun gyi cha lag gi nang tshan gyi sman so so'i mngon brjod dang ngos 'dzin shel dkar me long. Wooden printing block with Tibetan schpl. Toin Jambaldorj. gso byed bdud rtsi'i 'khrul med ngos 'dzin bzo rig me long du rnam par shar ba mdzes mtshar mig rgyan zhes bya ba bzhugs so. Wooden printing block with Tibetan script.
2. Research methods: Methods of textual analysis for ancient books and checklist methods were used in the study. Conclusion Gandhabhatra plant is used in traditional Mongolian medicinal practice from ancient times, the plant is called Veronica Incana L in Lalin. Also called Buural gandbadraa in Mongolian. On the other hand, the traditional medical doctors of China's Inner Mongolia and Tibet use Gandbadraa, the Latin name Gnaphalium affine D. Don. The appearance, shape, and color of the flowers of these two plants are different According to the research of the source, the main instruction and usage of the plants are the same: to deslroy benign tumors, lo remove poison, to treat colds, and to stop cough.

Background: It is important to determine and study in detail the action of the main chemical and secondary components contained in pink sedum against the disease. In particular, it is of great practical importance to clarify the use of the plant from ancient medical books and scriptures and to determine which of the six main diseases were used in traditional medicine. Purpose: Comparing the information of the ancient medical books written by Mongolian doctors and scientists, who wrote about Paeonia anomala L, to clarify the taste, power, and quality. Research materials and methods:
1. Research materials
- Sumbe khamba Ishbaljir. gso dpyad bdud rtsi 'i chu rgyun gyi cha lag gi nang tshan gyi sman so so'i mngon brjod dang ngos 'dzin shel dkar me long. Wooden printing block with Tibetan script.
- Toin Jambaldorj. gso byed bdud rtsi'i 'khrul med ngos "dzin bzo rig me long du rnam par shar ba mdzes mtshar mig rgyan zhes bya ba bzhugs so. Wooden printing block with Tibetan script.
2. Research methods
Methods of textual analysis for ancient books and checklist methods were used in the study. Conclusion The use of Paeonia anomala L. has been enriched with indications for use in traditional medicine, Mongolian folk medicine, Russian folk medicine, and Chinese, Japanese, and Korean traditional medicine. However, Sumbe khamba Ishbaljir in his book "ngos 'dzin shel dkar me long" wrote shogandha in Sanskrit and baspru in Tibetan. On the other hand, Toin Jambaldorj noted in his book "mtshar mig rgyan" wrote Paeonia anomala L. has warm and equable quality, so it can create power. Therefore, it is appropriate to write that the use of this plant in traditional Mongolian medicine is mainly used to treat back pain, chronic kidney disease, arthritis, uterine benign tumor, and bladder stone.

Introduction: Due to social transition and change of many societal activities in Mongolia, there is an increasing need for pharmaceutical drugs to treat anxiety, stress, fatigue and insomnia. It is considered that medicinal plants used in traditional medicine as sedatives, anti-anxiety and anti-depressive activities could be effective. Our study focuses on exploring optimal methods for preparing tinctures from Paeonia L. genus species, a sedative in Mongolian traditional medicine, and studying their phytochemical compositions. Materials and methods: Root and herb of Paeonia anomala L., grown in Mogod soum in Bulgan province, were used for the study.
Prepared herb and root were chopped and sieved into 1 mm, 2 mm, 3 mm, 4 mm and 5 mm sizes. Then from each size of two samples, a set was created with a 1:1 ratio. Finally 40 % ethanol (1:10 ratio with a set) was applied to prepare tincture through a traditional maceration method. The obtained tincture was analyzed for its color, quantity of biologically active compounds, dry residue, and absorption coefficient using an optimal particle size of samples, volume of the extractive liquid and tincture yield.
Tincture from the set of herb and root of Paeonia anomala L. was prepared using 3 methods, namely, by the traditional maceration; intensified maceration using the ultrasonic bath processing with 45 kHz at an ambient temperature; constant shaking in a heat of 30o-40oC. Conclusion The more optimal and effective method for preparing tincture from Paeonia anomala L. has been found to be the ultrasonic bath processing with 45 kHz for 6 h at an ambient temperature. Crude drug mixture of 2 mm sizes with 40% ethanol (where volume absorption coefficient was accounted to be 2.8) was mixed at a ratio of 1:10. In the obtained tincture, total phenols were determined as 0.58±0.003% in gallic acid equivalent and total monoterpene glycosides as 1.96±0.002% in paeoniflorin, which were the highest compared to other methods, indicating the method is the more optimal one.

Introduction: It is important to standardize a tincture prepared from the herb and root of Paeonia anomala L, which is widely used as a sedative in traditional medicine, based on the results of the studies its chemical composition, biological and pharmacological activities. Moreover, there is a need to carry out a quantitative stability testing in order to establish the ability to maintain quality under certain storage condition, shelf-life and to develop suitable packaging information. Materials and methods: Standardization parameters of prepared Paeonia anomala tincture have been evaluated according to WHO guidelines for the determination of crude drug material, plant preparations and finished products technical parameters, along with the Mongolian National Pharma- copoeian (MNP) and Russian XIV Pharmacopoeian methods for tincture. The color of a tincture, dry residue, special density, alcohol content, and microbiological content were determined according to the methods described in MNP. A qualitative analysis of biological active constituents has been evaluated by thin-layer chromatography, the total phenolic compounds were determined by the reaction with Folin Chicalteu reagent and total monoterpene glycosides by the reaction with hydroxylamine in alkaline solution and ferric (III) chloride following spectrophotometric examination, respectively.
The stability testing study was performed according to the “General requirements for the stability testing study of drug-active compounds” MNS-6439-2014 using the real-time methods for the standardization parameters of the tincture. Conclusions The standardized parameters of tincture prepared from the herb and root of Paeonia anomala L. were approved by the National Reference Laboratory for Food Safety analysis. Consequently, the Mongolian pharmacopoeian article on Paeonia anomala tincture was officially permitted under the number ҮФӨ-0327-2017.
The stability study of tincture was carried out according to the MNS-6439-2014 by real-time tests for 24 months which provided that the changes in tincture quality were within the permitted limits. Consequently, it can conclude that the storage condition and shelf-time of Paeonia anomala tincture are 2 years under not above 25°С.

Introduction: Sophora alopecuroides L has broadly been utilized in traditional medicine and all crude drugs including root, herb, and seed are used to treat numerous diseases. This herb is included in 181 Tibetan-Mongolian medicinal prescriptions and ranks 8^th among Mongolian medicinal plants in terms of frequency of administration. The S.alopecuroidesL . root standard was developed by the Institute of Traditional Medicine and Technology in 2017 and approved by “ҮФӨ-0307-2017”. Herb and seed are still used in medicine. Therefore, their standard parameters need to be determined and verified. Materials and methods: The quantitative pharmacognosy analysis of herb and seed was carried out in accordance with the methodology specified in the “General requirements for medicinal plant raw materials” of the National Pharmacopoeia of Mongolia. To determine the total alkaloid in standard matrine, a bromcresol green complex was formed, which was measured by spectrophotometer. Conclusion By developing, standards for the crude drugs of herb and seed of S.alopecuroides L. which are included in numerous medicinal prescriptions, will confirm the rationale for the use of medicinal raw materials and to expand the utilization’s possibilities.

Abstract More than 100 species of the Lactuca genus (Compositae family) are distributed across the northern hemisphere and also in temperate and warm regions of the world, namely 17 species in Europe, 51 in Asia, 43 in Africa, 12 in North and South America. In the Mongolian flora, four species of Lactuca, Lactuca unduiata Ledeb. Lactuca tatarica C.A.M, Lactuca serriola L. and Lactuca sibirica L Benth ex Maxim., are found. Their chemical constituents and biological activities have not been thoroughly studied. Plants of Lactuca species are widely used in traditional medicine, to treat acute respiratory infection and they have antipyretic and analgesic activities.
Since the 1980's, chemical constituents of Lactuca species started to be studied. Currently, about 148 secondary metabolites from more than 20 species were isolated and their structures elucidated, including sesquiterpene lactones-65 (43.91%), flavonoids-20 (13.51%), phenolic compounds-26 (17.56%), coumarins-2 (1.35%), triterpenes and steroids-20 (13.51%), lignans-5(3.39%), and other class of compounds-10 (6.75%). Among the isolated secondary metabolites, 9 were registered as new natural compounds. Extracts and isolated pure compounds from Lactuca species showed hepaprotective, antidiabetic, anticancer and antioxidant activities.