【Objective】 To explore the expression and clinical significance of prostate cancer tissue-specific lncRNAs. 【Methods】 The gene differences of 492 prostate cancer tissues and 152 adjacent tissues in TCGA and GEO genomic databases were analyzed with bioinformatics methods. A total of 5 lncRNAs were screened out, and their specificity in prostate tissues and impact on the prognosis of patients were analyzed. 【Results】 The 5 lncRNAs included PCAT14, PCA3, CTBP1-AS, DRAIC, and GPC5-AS1. PCAT14 and PCA3 were specifically expressed in prostate cancer tissues, and elevated expression was related to the prognosis. Moreover, they were well correlated with prostate cancer-specific antigens such as KLK3, AMACR, SLC45A3, and so on. GO function enrichment analysis and KEGG pathway enrichment analysis showed that the differential expression of PCA3 was closely related to phagocytosis, cell recognition, defense response to bacteria, immunoglobulin complex, Golgi apparatus, antigen binding, chemokine receptor binding, white matter digestion and absorption, renin-angiotensin system and other signaling pathways, while the differential expression of PCAT14 was closely related to the activity of Golgi apparatus and ion channels, renin secretion, cAMP signaling pathway, and gonadotropin secretion-related signaling pathway. 【Conclusion】 PCA3 and PCAT14 are specifically expressed in prostate cancer tissues, not in normal tissues, which can be used as potential indicators for the diagnosis of prostate cancer.

Objective: This study examined the impact of PRRSV NADC30-like strains on pig farms in Fujian, China. Methods: The effectiveness of strategic management protocols, including herd closure, medication programs, monitoring of processing fluids (pig testicular fluid), and collection of production data, were analyzed. The prevalent strain in the pig farm was identified as a NADC30-like strain of the PRRSV through genetic sequencing comparison analysis. Results: The quantitative real-time reverse-transcription polymerase chain reaction results showed that the PRRSV cycle threshold (Ct) values of the processing fluid samples were relatively low from September to early October 2021. After implementing the intervention measures (October 2021), the Ct value increased gradually and reached a negative in March 2022, lasting six months. In addition, the average survival rate of the pigs before the intervention was 84.1%, while the average survival rate after the intervention was 93.1%. Conclusions and Relevance: The use of 12-month intervals for pig herd closure, drug planning, and other strategic management agreements (multi-point production and active monitoring of production data, McREBEL) helped stabilize the subsequent pig farm production, providing a basis for clinical disease prevention and control.

Objective: This study examined the impact of PRRSV NADC30-like strains on pig farms in Fujian, China. Methods: The effectiveness of strategic management protocols, including herd closure, medication programs, monitoring of processing fluids (pig testicular fluid), and collection of production data, were analyzed. The prevalent strain in the pig farm was identified as a NADC30-like strain of the PRRSV through genetic sequencing comparison analysis. Results: The quantitative real-time reverse-transcription polymerase chain reaction results showed that the PRRSV cycle threshold (Ct) values of the processing fluid samples were relatively low from September to early October 2021. After implementing the intervention measures (October 2021), the Ct value increased gradually and reached a negative in March 2022, lasting six months. In addition, the average survival rate of the pigs before the intervention was 84.1%, while the average survival rate after the intervention was 93.1%. Conclusions and Relevance: The use of 12-month intervals for pig herd closure, drug planning, and other strategic management agreements (multi-point production and active monitoring of production data, McREBEL) helped stabilize the subsequent pig farm production, providing a basis for clinical disease prevention and control.

Background::Pulmonary arterial hypertension (PAH) is characterized by excessive proliferation of small pulmonary arterial vascular smooth muscle cells (PASMCs), endothelial dysfunction, and extracellular matrix remodeling. G protein-coupled receptor kinase 2 (GRK2) plays an important role in the maintenance of vascular tone and blood flow. However, the role of GRK2 in the pathogenesis of PAH is unknown.Methods::GRK2 levels were detected in lung tissues from healthy people and PAH patients. C57BL/6 mice, vascular smooth muscle cell-specific Grk2-knockout mice ( Grk2?SM22), and littermate controls ( Grk2flox/flox) were grouped into control and hypoxia mice ( n = 8). Pulmonary hypertension (PH) was induced by exposure to chronic hypoxia (10%) combined with injection of the SU5416 (cHx/SU). The expression levels of GRK2 and Yes-associated protein (YAP) in pulmonary arteries and PASMCs were detected by Western blotting and immunofluorescence staining. The mRNA expression levels of Grk2 and Yes-associated protein ( YAP) in PASMCs were quantified with real-time polymerase chain reaction (RT-PCR). Wound-healing assay, 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, and 5-Ethynyl-2′-deoxyuridine (EdU) staining were performed to evaluate the proliferation and migration of PASMCs. Meanwhile, the interaction among proteins was detected by immunoprecipitation assays. Results::The expression levels of GRK2 were upregulated in the pulmonary arteries of patients with PAH and the lungs of PH mice. Moreover, cHx/SU-induced PH was attenuated in Grk2?SM22 mice compared with littermate controls. The amelioration of PH in Grk2?SM22 mice was accompanied by reduced pulmonary vascular remodeling. In vitro study further confirmed that GRK2 knock-down significantly altered hypoxia-induced PASMCs proliferation and migration, whereas this effect was severely intensified by overexpression of GRK2. We also identified that GRK2 promoted YAP expression and nuclear translocation in PASMCs, resulting in excessive PASMCs proliferation and migration. Furthermore, GRK2 is stabilized by inhibiting phosphorylating GRK2 on Tyr86 and subsequently activating ubiquitylation under hypoxic conditions. Conclusion::Our findings suggest that GRK2 plays a critical role in the pathogenesis of PAH, via regulating YAP expression and nuclear translocation. Therefore, GRK2 serves as a novel therapeutic target for PAH treatment.

Objective: This study examined the impact of PRRSV NADC30-like strains on pig farms in Fujian, China. Methods: The effectiveness of strategic management protocols, including herd closure, medication programs, monitoring of processing fluids (pig testicular fluid), and collection of production data, were analyzed. The prevalent strain in the pig farm was identified as a NADC30-like strain of the PRRSV through genetic sequencing comparison analysis. Results: The quantitative real-time reverse-transcription polymerase chain reaction results showed that the PRRSV cycle threshold (Ct) values of the processing fluid samples were relatively low from September to early October 2021. After implementing the intervention measures (October 2021), the Ct value increased gradually and reached a negative in March 2022, lasting six months. In addition, the average survival rate of the pigs before the intervention was 84.1%, while the average survival rate after the intervention was 93.1%. Conclusions and Relevance: The use of 12-month intervals for pig herd closure, drug planning, and other strategic management agreements (multi-point production and active monitoring of production data, McREBEL) helped stabilize the subsequent pig farm production, providing a basis for clinical disease prevention and control.

Objective: This study examined the impact of PRRSV NADC30-like strains on pig farms in Fujian, China. Methods: The effectiveness of strategic management protocols, including herd closure, medication programs, monitoring of processing fluids (pig testicular fluid), and collection of production data, were analyzed. The prevalent strain in the pig farm was identified as a NADC30-like strain of the PRRSV through genetic sequencing comparison analysis. Results: The quantitative real-time reverse-transcription polymerase chain reaction results showed that the PRRSV cycle threshold (Ct) values of the processing fluid samples were relatively low from September to early October 2021. After implementing the intervention measures (October 2021), the Ct value increased gradually and reached a negative in March 2022, lasting six months. In addition, the average survival rate of the pigs before the intervention was 84.1%, while the average survival rate after the intervention was 93.1%. Conclusions and Relevance: The use of 12-month intervals for pig herd closure, drug planning, and other strategic management agreements (multi-point production and active monitoring of production data, McREBEL) helped stabilize the subsequent pig farm production, providing a basis for clinical disease prevention and control.

Objective:To analyze the influential factors of hypothermia in congenital heart disease (CHD) after cardiopulmonary bypass (CPB) rewarming using the decision tree model, thus providing theoretical basis for medical staff.Methods:A total of 711 CHD children who underwent surgery in the Shanghai Children′s Medical Center from January 1, 2019 to April 30, 2019 were retrospectively analyzed.A decision tree model was established to predict the risk factors for hypothermia in CHD children following CPB.Results:The decision tree model showed that CPB program, preoperative nutrition score and body surface area were the high-risk factors for hypothermia in CHD children after CPB rewarming.The accuracy, sensitivity, specificity of the decision tree model were 86.45%, 77.14% and 90.97%, respectively, and the area under the receiver operating characteristic curve was 0.851(95% CI: 0.798-0.904). Conclusions:Decision tree model has a high application value in predicting hypothermia in CHD children following CPB.It contributes to identify the influential factors of hypothermia, and provides references for performing preventive treatment and nursing measures to control the risk of hypothermia.

Humans ; Hypothermia ; Perioperative Care ; Intraoperative Complications ; Patients ; Body Temperature

ObjectiveTo investigate the relationship between the levels of D-dimer and inflammatory factors C-reactive protein（CRP）and prognosis in patients with 2019 novel coronavirus-infected pneumonia（COVID-19）.Methods The clinical data of a total of 242 patients with COVID-19 who were treated in hospital from February 4th 2020 to February 18th 2020 were analyzed retrospectively. According to the classification standard,the patients with COVID-19 were divided into common patients(131 cases), severe patients（88 cases）, and critical patients（23 cases）. The difference between the levels of D-dimer and CRP in patients with pneumonia of different severity and clinical outcomes was compared and the correlation between D-dimer and CRP was analyzed.ResultsThe levels of D-dimer and CRP in severe and critical patients were significantly higher than those in common patients(P<0.05). The levels of CRP in critical patients were significantly higher than those in severe patients(P<0.05). These two indicator levels of patients who died of COVID-19 within 30 dayswere significantly higher than those who survived. Pearson correlation analysis showed that the levels of D-dimer were positively correlated with the levels of CRP(r=0.649，P<0.05).ConclusionD-dimer and CRP are highly expressed in severe and critical patients, and the severe abnormality of the two indicators in the early stage of COVID-19 predicted the poor prognosis. D-dimer and CRP have certain clinical value in evaluating the severity and prognosis of COVID-19.

OBJECTIVE: To investigation the types and frequencies of thalassemia gene mutations in pregnant population in Nanping area of Fujian Province, so as to provide a basis for prevention and control of birth children with moderate and severe thalassaemia in this area. METHODS: The genotyping of α and β thalassemia was performed using the gap-PCR (gap-PCR) technique combined with reverse dot blot (RDB). The genotyping test was performed by Gap-PCR for three rare deficient thalassemia. The cases with negative detection were further detected by Sanger sequencing method, so as to identify rare α or β thalassemia mutation. RESULTS: 1120 specimens were genotyped for thalassemia, out of them 547 thalassemia genes were determined. The detection rate was 48.8% (547/1120). 340 specimens were diagnosed as α thalassemia, and the detection rate was 30.6%, including 266 cases of --/αα, 44 cases of -α/αα, 12 cases of -α/αα, 8 cases of αα/αα,. 3 cases of Hb H disease ( 2 cases of --/-α, 1 case of --/-α), 2 cases of αα/αα, 2 cases of αα/αα, 1 case of -α/-α, and 1 case of -α/αα. Also, they contain 11 cases of rare α thalassemia, 8 kinds of rare types of α thalassemia mutations in combination, such as 4 cases of αα/αα, 1 case of αα/αα, 1 case of αα/αα, 1 case of αα/αα, 1 case of αα/αα, 1 case of αα/αα, 1 case of αα/αα, and 1 case of --/αα. Among them, 5 α mutation sites were first reported, namely αα, αα, αα, αα and αα; 2 α thalassemia mutation sites: αα and -- were detected again in the Chinese population, respectively. 188 specimens were diagnosed as β thalassemia with a detection rate of 16.8%. Among them, 68 cases of β/βN, 47 cases of β/βN, 20 cases of β/βN, 17 cases of β/βN, 7 cases of β/βN, 7 cases of βE/βN, 3 cases of β/βN and 2 cases of β/βN. And 17 cases were diagnosed as rare β thalassemia, 8 kinds of rare types were β thalassemia mutations in combination. There were 4 cases of β/βN, 3 cases of β/βN, 3 cases of β/βN, 2 cases of β/βN, 2 cases of β/βN, 1 case of β/βN, 1 case of β/βN, 1 case of β/βN. Among them, 3 β thalassemia mutation sites were reported for the first time, namely β, β and β; it was found that in the Chinese population as β, β, β, β, and β, respectively. 19 cases were diagnosed as αβ-complex thalassemia, out of which 15 types of thalassemia mutation combinations were detected. They contain 2 cases of rare αβ-complex thalassemia, which are αα/αα complex β/βN, αα in α1/αα complex β/βN. CONCLUSION The types of thalassemia gene mutations in Nanping area of Fujian province are genetically heterogeneous. The prevention and control strategies of thalassaemia in this area should be based on the prevention and treatment of common α thalassemia and β thalassaemia. And the attention should be paid to the types of rare and unknown gene mutations using screening and testing method.
China ; Female ; Genotype ; Humans ; Mutation ; Pregnancy ; Thailand ; alpha-Thalassemia ; genetics ; beta-Thalassemia ; genetics