Laboratory animals are essential in scientific research and experimental teaching in colleges and universities. Disciplines such as life sciences, medicine, pharmacy, chemistry, and biomedical engineering heavily rely on animal experiments. The standardized barrier environmental facility for laboratory animals provides a fundamental platform for stable, scientific, and reliable animal experiment results. Rigorous access management for such facilities is a vital safeguard for maintaining standardized operations of facilities, controlling the quality and stability of laboratory animals, mitigating pathogen contamination risks among animals and laboratory staff, and preventing biosecurity incidents such as zoonotic disease outbreaks. Taking the small-scale barrier facilities for laboratory rats and mice at Nanjing University's Xianlin Campus, operational since 2019, as an example, this study focuses on the safety access management system of these facilities. Based on five years of operational data and accumulated experience in studying and optimizing the access management system, this study, from the perspectives of management system development and the formulation and implementation of standard operating procedures, reviews five aspects of access management: personnel access, animals access, material access, equipment access, and air circulation control. Furthermore, these aspects are systematically analyzed and summarized to serve as a reference for the construction and management of the laboratory animal facilities in universities, while also contributing to scientific research, public health security, and the well-being of experimental personnel.

Objective To investigate the relationship between interleukin-1β (IL-1β) and miR-185-5p in the process of joint injury in acute gouty arthritis (AGA). Methods The serum miR-185-5p levels of 89 AGA patients and 91 healthy volunteers were detected by real-time quantitative PCR. The correlation between miR-185-5p expression level and VAS score or IL-1β expression level was evaluated by Pearson correlation coefficient method. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of miR-185-5p in AGA. THP-1 cells were induced by sodium urate (MSU) to construct an in vitro acute gouty inflammatory cell model. After the expression level of miR-185-5p in THP-1 cells was upregulated or downregulated by transfection of miR-185-5p mimics or inhibitors in vitro, inflammatory cytokines of THP-1 cells, such as IL-1β, IL-8 and tumor necrosis factor α (TNF-α), were detected by ELISA. The luciferase reporter gene assay was used to determine the interaction between miR-185-5p and the 3'-UTR of IL-1β. Results Compared with the healthy control group, the expression level of serum miR-185-5p in AGA patients was significantly reduced. The level of serum miR-185-5p was negatively correlated with VAS score and IL-1β expression level. The area under the curve (AUC) was 0.905, the sensitivity was 80.17% and the specificity was 83.52%. Down-regulation of miR-185-5p significantly promoted the expression of IL-1β, IL-8 and tumor necrosis factor (TNF-α), while overexpression of miR-185-5p showed the opposite results. Luciferase reporter gene assay showed that IL-1β was the target gene of miR-185-5p, and miR-185-5p negatively regulated the expression of IL-1β. Conclusion miR-185-5p alleviates the inflammatory response in AGA by inhibiting IL-1β.
Humans ; 3' Untranslated Regions ; Arthritis, Gouty/genetics* ; Interleukin-1beta/genetics* ; Interleukin-8 ; Luciferases ; MicroRNAs/genetics* ; Tumor Necrosis Factor-alpha

BACKGROUND:Cerebral ischemic stroke is one of the main fatal and disabling diseases in the clinic,but only a few patients benefit from vascular recanalization in time,so it is urgent to explore new and effective therapy.As one of the critical pathological changes of ischemic stroke,the glial scar formed mainly by astrocytes is one major cause that hinders axonal regeneration and neurological recovery at the late stage of stroke. OBJECTIVE:To elucidate the pathological process and crucial signal regulatory mechanism of astrocytes in the formation of glial scar after ischemic stroke,as well as the potential therapeutic targets,to provide a theoretical reference for intervening astrocytic scar formation against ischemic stroke effectively,and novel strategies for promoting post-stroke rehabilitation. METHODS:The relevant articles published in CNKI,PubMed and Web of Science databases from 2010 to 2022 were retrieved.The search terms were"Ischemic stroke,Brain ischemi*,Cerebral ischemi*,Astrocyt*,Astroglia*,Glial scar,Gliosis,Astrogliosis"in Chinese and English.Finally,78 articles were included after screening and summarized. RESULTS AND CONCLUSION:(1)Astrocytes play an important role in the maintenance of central nervous system homeostasis.After ischemic stroke,astrocytes change from a resting state to an active state.According to the different severities of cerebral ischemic injury,astrocyte activation changes dynamically from swelling and proliferation to glial scar formation.(2)Mature astrocytes are stimulated to restart the cell cycle,then proliferate and migrate to lesions,which is the main source of the glial scar.Neural stem cells in the subventricular zone,neuron-glial antigen 2 precursor cells and ependymal precursor cells in the brain parenchyma can also differentiate into astrocytes.Endothelin-1,aquaporin 4,ciliary neurotrophic factor and connexins are involved in this process.In addition,chondroitin sulfate proteoglycan,as the main component of the extracellular matrix,forms the dense glial scar barrier with proliferated astrocytes,which hinders the polarization and extension of axons.(3)Activation or inhibition of crucial signal molecules involved in astrocyte activation,proliferation,migration and pro-inflammation functions regulate the glial scar formation.Transforming growth factor beta 1/Smad and Janus kinase/signal transducer and activator of transcription 3 are classical pathways related to astrogliosis,while receptor-interacting protein 1 kinase and glycogen synthase kinase 3β are significant molecules regulating the inflammatory response.However,there are relatively few studies on Smad ubiquitination regulatory factor 2 and Interleukin-17 and their downstream signaling pathways in glial scar formation,which are worthy of further exploration.(4)Drugs targeting astrogliosis-related signaling pathways,cell proliferation regulatory proteins and inflammatory factors effectively inhibit the formation of glial scar after cerebral ischemic stroke.Among them,the role of commonly used clinical drugs such as melatonin and valproic acid in regulating glial scar formation has been verified,which makes it possible to use drugs that inhibit glial scar formation to promote the recovery of neurological function in patients with stroke.(5)Considering the protective effects of glial scar in the acute phase,how to choose the appropriate intervention chance of drugs to maintain the protective effect of the glial scar while promoting nerve regeneration and repair in the local microenvironment is the direction of future efforts.

Objective To analyze the effect of spousal synchronous exercise and diet intervention on maternal and neonatal outcomes in overweight pregnant women with gestational diabetes mellitus(GDM).Methods We divided 120 overweight pregnant women with GDM into intervention and control groups(n= 60 per group),who received spousal synchronous and routine exercise and diet interventions,respectively.The age,body mass index(BMI),and levels of fasting blood glucose(FPG),2-hour postprandial blood glucose(2 h-PG),and glycosylated hemoglobin(HbA1c)at the first prenatal examination were recorded.The levels of FPG,2 h-PG,and HbA1c at the diagnosis of GDM and gestational week 28,delivery mode,and incidence of complications during the delivery were compared between the groups.The self-rating depression scale and self-rating anxiety scale were used to evaluate the women's mental status after delivery.The body weight,blood glucose level,Apgar score,and incidence of complications including macrosomia,hypoglycemia,and asphyxia in the new-borns were compared between the groups.Results The groups did not differ significantly in term of age,BMI,FPG,2 h-FPG,or HbA1c at GDM diagnosis(P>0.05).At gestational week 28,the FPG,2 h-FPG,and HbA1c levels were significantly lower than those before the intervention in both groups,and were also significantly lower in the intervention group than in the control group(P<0.05).Com-pared with the control group,the proportion of natural delivery was significantly higher and the incidence of complications was sig-nificantly lower in the intervention group(P<0.05).The anxiety and depression status were significantly better in the intervention group than in the control group(P<0.05).Compared with the control group,the frequency of macrosomia and blood glucose levels were significantly lower and the Apgar scores were significantly higher in the intervention group(P<0.05).Conclusion Spousal synchronous exercise and diet intervention may effectively decrease the blood glucose and HbA1c levels in overweight pregnant women with GDM,decrease the incidence of maternal and neonatal complications,and improve pregnant women's mental status.

Osteoporotic proximal humeral fracture (OPHF) is one of the common osteoporotic fractures in the aged, with an incidence only lower than vertebral compression fracture, hip fracture, and distal radius fracture. OPHF, secondary to osteoporosis and characterized by poor bone quality, comminuted fracture pattern, slow healing, and severely impaired shoulder joint function, poses a big challenge to the current clinical diagnosis and treatment. In the field of diagnosis, treatment, and rehabilitation of OPHF, traditional Chinese and Western medicine have accumulated rich experience and evidence from evidence-based medicine and achieved favorable outcomes. However, there is still a lack of guidance from a relevant consensus as to how to integrate the advantages of the two medical systems and achieve the integrated diagnosis and treatment. To promote the diagnosis and treatment of OPHF with integrated traditional Chinese and Western medicine, relevant experts from Orthopedic Expert Committee of Geriatric Branch of Chinese Association of Gerontology and Geriatrics, Youth Osteoporosis Group of Orthopedic Branch of Chinese Medical Association, Osteoporosis Group of Orthopedic Surgeon Branch of Chinese Medical Doctor Association, and Osteoporosis Committee of Shanghai Association of Integrated Traditional Chinese and Western Medicine have been organized to formulate Expert consensus on the diagnosis and treatment of osteoporotic proximal humeral fracture with integrated traditional Chinese and Western medicine ( version 2024) by searching related literatures and based on the evidences from evidence-based medicine. This consensus consists of 13 recommendations about the diagnosis, treatment and rehabilitation of OPHF with integrated traditional Chinese medicine and Western medicine, aimed at standardizing, systematizing, and personalizing the diagnosis and treatment of OPHF with integrated traditional Chinse and Western medicine to improve the patients ′ function.

It is believed that qi and blood disharmony is the key pathogenesis of essential hypertension， for which mildly regulating qi and blood is recommended. According to the different pathological states and related causes of qi and blood disorders， essential hypertension can be divided into five syndrome types for differentiation and treatment. In terms of blood deficiency and liver constraint， it is recommended to nourish the blood and soften the liver， rectify qi to resolve constraint， using Xiao Yao Powder （逍遥散） or self-made Qihua Jieyu Decoction （七花解郁汤）. For qi stagnation and blood stasis， the method of regulating qi and harmonizing blood， dissolving stasis and unblocking collaterals should be used， with self-made Guitao Tongluo Decoction （桂桃通络汤）. For phlegm-dampness internal obstruction， it is recommended to move qi and promote urination， dissolve phlegm and eliminate dampness， using self-made Zhuanqi Sanzhuo Formula （转气散浊方）. For binding of phlegm and stasis， dissolving phlegm and dispelling stasis， dredging the bowels and directing the turbid downward is advised， and self-made Sanren Tiaozhi Formula （三仁调脂方） can be used. In terms of deficiency of both qi and blood， it is recommended to boost qi and nourish blood， supplement deficiency and consolidate the root， using Gui Pi Decoction （归脾汤） or self-made Shenqi Zaizao Decoction （参芪再造汤）.

The Republic of China period （1912—1949） was an important stage of transition from traditional herbalism to clinical Chinese materia medica. The clinical application of Chinese materia medica became the focus of academic attention. During the Republic of China period， the research objectives， methods， and content of clinical Chinese materia medica were clarified， and the basic framework of Chinese materia medica was established through efficacy classification and item description. Based on the historical background at that time， striving for survival in adversity were the internal factors driving the construction of clinical Chinese materia medica， and the rise of academic education and the popularity of traditional Chinese medicine journals provided favorable conditions for the development of clinical Chinese materia medica. The clinical Chinese materia medica during the Republic of China period gradually formed a theoretical structure and core content that was distinctly different from that of traditional herbal medicine， which was integrated with the scientific research of Chinese materia medica and promoted the continuous development of Chinese materia medica together with other subdisciplines， presenting distinctive characteristics of the times and important academic significance.

Glioma is the most common malignancy of the central nervous system, originating mainly from glial cells. Because of its highly aggressive nature, glioma has one of the highest rates of death among all types of cancer. Therefore, it is very important to develop new therapeutic approaches and drugs for glioma treatment. Instead of activate the telomerase, approximately 30% of glioma use alternative lenthening of telomere (ALT) to maintain telomere length. The mechanism of ALT development is poorly understood, however, some genetic mutations have been reported to induce the development of ALT glioma, such as ATRX, IDH1, p53, etc. The lack of ALT glioma cell lines and preclinical ALT glioma models has limited the mechanistic studies of ALT glioma. Therefore, this review listed ALT glioma cell lines that derived from primary culture or gene editing in the last decade, as well as the xenografted animal models established by ALT glioma cell lines, and discussed the role and significance these cell and animal models play in preclinical studies.

Objective To investigate the association of whole blood nucleophosmin 1(NPM1),monocyte chemotac-tic protein-1(MCP-1),and intestinal flora with gastric cancer progression and prognosis.Methods A total of 120 patients with gastric cancer who were admitted to the Nanyang Second People's Hospital from May 2018 to May 2020 were selected as the gastric cancer group,120 patients with benign gastric lesions underwent gastroscopy in the same period were selected as the benign gastric lesions group,and 120 patients with healthy physical examinations in the same period were selected as the control group.RT-qPCR was used to detect mRNA expression of NPM1 and MCP-1.The intestinal microbiota was examined by MicroScan microbial identification analysis system;The correla-tion between NPM1,MCP-1 and intestinal microbiota was analyzed by Pearson method.Multivariate COX regression was used to analyze the influencing factors of prognosis in gastric cancer patients.Results The level of NPM1 mRNA,exuberance of Bifidobacterium and Lactobacillus in the control group,gastric benign lesion group,and gastric cancer group decreased sequentially(P＜0.05),whereas MCP-1 mRNA,Enterococcus,and Escherichia coli increased sequentially(P＜0.05).Serum NPM1 mRNA,Bifidobacterium and Lactobacillus in stage Ⅲ-Ⅳ of gastric cancer were obviously lower than those found in stage Ⅰ-Ⅱ(P＜0.05),while MCP-1 mRNA,Enterococcus and Escherichia coli were obviously more than those found in stage Ⅰ-Ⅱ(P＜0.05).Pearson correlation analysis showed that,there was a negative correlation between serum NPM1 mRNA and MCP-1 mRNA levels in gastric cancer patients(P＜0.05),NPM1 mRNA was positively correlated with Bifidobacterium and Lactobacillus(P＜0.05)and negatively correlated with Enterococcus and Escherichia coli(P＜0.05).MCP-1 mRNA was negative-ly correlated with the quantity of Bifidobacterium and Lactobacillus(P＜0.05)but positively correlated with Entero-coccus and Escherichia coli(P＜0.05).The level of NPM1 mRNA,quantity of Bifidobacterium and Lactobacillus in the poor prognosis group were obviously lower than those found in the good prognosis group(P＜0.05),while the level of MCP-1 mRNA,quantity of Enterococcus and Escherichia coli were obviously higher than those in the good prognosis group(P＜0.05).COX regression analyses showed that MCP-1 mRNA,Enterococcus and Escherichia coli were risk factors affecting the prognosis of gastric cancer patients(P＜0.05),while NPM1 mRNA,Bifidobacterium,and Lactobacillus were protective factors(P＜0.05).Conclusions Decreased level of NPM1,decreased counting of Bifidobacterium intestinalis and Lactobacillus,increased level of MCP-1,increased counting of Enterococcus and Escherichia coli are strongly associated with gastric cancer progression and poor prognosis.

Bipolar HF leakage current testing was carried out for two pieces of HF surgical equipment respectively by using a combination of different equipment and different connection methods according to the test method in GB 9706.202-2021 Medical electrical equipment-Part 2-2:Particular requirements for the basic safety and essential performance of high frequency surgical equipment and high frequency surgical accessories.It's pointed out relatively accurate measurements could be obtained when the HF analyzer was accessed to the circuit only as a test resistor and/or ammeter.References were provided for bipolar HF leakage current detection in HF surgical equipment.[Chinese Medical Equipment Journal,2024,45(4):88-92]