Introduction: The research is based on the study of the action of traditional drugs that have important anti-bacterial and anti-inflammatory effects, as well as their low cost and low side effects in medical practice. Material and methods: We conducted the research based on the material base of Otoch Manramba University’s pharmaceutical chemistry laboratory. We took Banzi 12 medicinal powder as research object, made in Manba Datsan pharmaceutical plant. The content of alkaloids, flavonoids, coumarins and total phenolic compounds in Banzi 12 medicinal powder drugs was determined by spectrophotometry, extractive substances by titrimetric method, weighted content of extractable substances, heavy metal content by atomic absorption spectrophotometry and bacteriological analysis according to the methodology specified in MNF. The effect on acute inflammation (U.E. Strelnikov) was determined according to the method of formalin causing an edema. Conclusion
1. The highest concentrations of flavonoids70% alcohol content is11.5%±0.001, preservatives 14.5%±0.72, and polyphenolic compounds 1.68%±0.005were used to determine the content of biologically active substances in Banzi 12medicinal powderdrug.
2. Banzi12medicinal powderdrug produced by Manba Datsan traditional medicine factory used in the study meets the requirements of MNS 5586: 2006 for traditional medicine.
3. Formalin has been shown to have anti-inflammatory action for acute inflammation.

BACKGROUND OF STUDY: According to WHO report estimation, 76.3 million people have been diagnosed with disorder related to alcohol use which has been root cause for over 60 types of disease in the world.1 Smoking causes death to4 million people every year, 11 people every day and 6 every minute. This number is estimated to double reaching 10 million by 2030.2 The study conducted by the ‘World Vision Mongolia’ international organization and the Anti-alcoholism and Drug Association of the Ministry of Justice show 76% of all teenagers has drunk alcohol to some extent. AIMS: To determine alcohol, tobacco and psychoactive drug use by school teenagers of Sukhbaatar district in Ulaanbaatar MATERIAL AND METHODOLOGY: The survey on inquiry of alcohol, tobacco and psychoactive drug use (ASSIST) was conducted among a total of randomly selected 800 (374 boys, 426 girls) students of 8-12th grade from 7 secondary schools of Sukhbaatar district. Statistical data was put into Microsoft office applications (excel, word) and processed by SPSS application with 95% of probability of information and 5.0% of trust level. RESULT OF STUDY: Out of all respondents 32% (256) use tobacco, 43.8% (350)alcoholic drinks, 2.6% (21)cannabis,1.1% (9) cocaine, 1.5% (12) amphetamine, 3%(24)volatile substance, 4.3% (34)sedative and tranquilizer drug, 0.6% (5)hallucinogenic drug, 0.5% (4) opioid, and 0.1% (1) uses other types of drug. Among the survey participants, there are a total of 214 teenagers (in duplicated number) who need short-term action and 2 teenagers who are smoker and need intensive treatment. CONCLUSION: Among total teenagers, the survey participants, 1 in 3 used tobacco, 1 in 2 alcoholic drinks and 1 in 7 used psychoactive drug. Male students smoke twice more than female students and there is no difference in use of alcoholic drink in both sexes. The alcohol and tobacco use among teenagers at the age of 12-18 who participated in the survey showed that they use more when they grow older. 1 in 4 students of all participants belongs to a group with average risk and needs intensive treatment.

Introduction: In 1814 Irish surgeon Abraham Coll first introduced distal radial bone fracture in clinical practice as a colles fracture. It is one of the most common fractures account for 10-20% of the total respectively fracture. Case of Colles fracture has being increased in the developed country year by year besides the increasing number of elderly patients. Depending on severity displaced of the fracture, management includes closed reduction or surgical procedure. The aim of study was to study result of risk factors that influence the closed reduction management of the Colles fracture. Materials and Methods: From hospital based population 80 patients aged between 5-76 years (mean age 47.31 years, male 61.25%, female 38.7%) were recruited by cross sectional and randomized method. Participants were divided into displaced and non-displaced groups which confirmed by refractive index difference on X-ray. Results: The risk factors that influence the colles fracture closed reduction management was osteoporosis (p=0.38), menopause (r=0.18, p=0.27), calcium supplement intake (r=0.21, p=0.05), received hospital care in 24 hour (p=0.39), apply plaster (p=0.64), hand sling immobilizer brace (p=0.5) and physical therapy (p=0.5). Conclusion: Osteoporosis and menopause were the risk factors that influenced the closed reduction management of Colles fracture. The patient cases that not receiving emergency medical care in first 24 hours, not applying plaster, not using the hand sling immobilizer brace and not receiving physical therapy was risk factors for extending the closed reduction management of the Colles fracture.

Background:We determined lipid parameters which are the leading causes of developing cardiovascular diseases and atherosclerosis and investigated the increased serum triglyceride level which is referred to as atherogen triad , decreased high-density lipoprotein and increased low- density lipoprotein associating with age and sex.Method:237 people who were done lipid level parameter tests in the Central Hospital Laboratory were involved in the study. We identifed cholesterol, triglyceride, lowdensity lipoprotein (LDL) and high-density lipoprotein ( HDL) (COBASC-311,ROCHE,Germany) which are the lipid parameters using full automatic apparatus .Results:While serum cholesterol level increased in the ages between 40-60 and has a tendency to decrease in the further, HDL level was decreasing as the age grew. Serum LDL level has strong direct relevance to cholesterol (r=0.720, p<0.01) and weak relevance to triglyceride (r=0.189,p<0.01). As for the sex, high- density lipoprotein level decreased for men with statistical importance (p<0.05) and there was no signifcant difference in other parameters. LDL level increased for 81.8% , increased cholesterol for 51.1%, raised triglyceride for 27.9% comparing with the normal rate but HDL level was decreased for 32.2% of the participants in the study. These changes mostly occurred at the ages of 40-60. Atherogen triad occurred for 16.5% of the participants and 13(33.3%) of whom were male and 26(66.7%) female.Conclusion:LDL and cholesterol level raised more than the normal rate comparing with other lipid parameters particularly as for 40-60 aged people and atherogen triad which leads to develop atherosclerosis occurs in 16.5% of all adults in the study.

Kidney transplantation is the best alternative treatment for end-stage renal disease and health-related quality of life and survival of the patients are improved compared with dialysis. Worldwide, more than 1.4 million patients with CKD receive renal replacement therapy with incidence growing by approximately 8% annually.1 Unfortunately, despite significant improvement in graft function, kidney transplants can still fail due to acute rejection and chronic allograft nephropathy.2 Kidney biopsy after transplantation, which has evaluated by Banff 09 classification is usefull method for diagnose of transplanted kidney disease.3,4Kidney graft rejection was diagnosed in 10 renal allograft biopsy specimens (bs) obtained from transplant patients followed up at our institute between 2015 and 2016. All specimens were evaluated as satisfactory which show more than 8 glomerulus under the light microscopy. Each renal cortical tissue was divided into two tips: one piece for routine H&E stain and special stains, including Masson’s trichrome, and PAS stain; another piece for immunofluorescence by frozen section, which were stained with IgA, IgM, IgG and complement component (C3, C4, C1q, C4d). All the renal biopsies were examined by the same pathologist.Out of 117 transplantations, 10 episodes of rejection selected. Among the 10 patients, 30% had an acute T cell rejection and 70% had a chronic allograft nephropathy. Interstitial inflammation (i1-7) was present in 7 bs (70%), tubulitis (t1-4,t2-2) in 6 bs (60%), transplant glomerulitis (g1-1, g2-2, g3-1) in 4 bs (40%), transplant interstitial fibrosis (ci1-2, ci2-2, ci3-2) in 6 bs (60%), tubular atrophy (ct1-6, ct2-2, ct3-1) in 9 bs (90%), mesangial matrix increase (mm1-5) in 5 bs (50%), vascular fibrosis intimal thickeness (cv1-3) in 3 bs (30%), arteriolar hyaline thickening (ah1-5) in 5 bs (50%), tubulitis (ti1-6, ti2-3, ti3-1) in 10 bs (100%) and peritubular capillaritis (ptc1-1, ptc2-2, ptc3-1) in 4 bs (40%). C4d deposition was present very mild in wall of the vessels and peritubular capillaries. Because of not good working Methenamin silver stain, we couldn’t demostrate glomerular basement membrane changes (cg) fully.We suggest that histopathological changes of transplant glomerulopathy might be accompanied by inflammation of the microvasculature, such as transplant glomerulitis and peritubular capillaritis. C4d deposition in the wall of the vessels and peritubular capillaritis is not always present in biopsy specimens of transplant glomerulopathy.

Introduction: In 1814 Irish surgeonAbraham Coll first introduced distal radialbone fracture in clinical practice as a collesfracture. It is one of the most commonfractures account for 10-20% of the totalrespectively fracture. Case of Colles fracturehas being increased in the developed countryyear by year besides the increasing numberof elderly patients. Depending on severitydisplaced of the fracture, managementincludes closed reduction or surgicalprocedure. The aim of study was to studyresult of risk factors that influence theclosed reduction management of the Collesfracture.Materials and Methods: From hospitalbased population 80 patients aged between5-76 years (mean age 47.31 years, male61.25%, female 38.7%) were recruited bycross sectional and randomized method.Participants were divided into displaced andnon-displaced groups which confirmed byrefractive index difference on X-ray.Results: The risk factors that influence thecolles fracture closed reduction managementwas osteoporosis (p=0.38), menopause(r=0.18, p=0.27), calcium supplement intake(r=0.21, p=0.05), received hospital care in24 hour (p=0.39), apply plaster (p=0.64),hand sling immobilizer brace (p=0.5) andphysical therapy (p=0.5).Conclusion: Osteoporosis and menopausewere the risk factors that influenced theclosed reduction management of Collesfracture. The patient cases that not receivingemergency medical care in first 24 hours,not applying plaster, not using the hand slingimmobilizer brace and not receiving physicaltherapy was risk factors for extending theclosed reduction management of the Collesfracture.

BackgroundMonos Pharm LLC has been started production of Dentamon which is an elixir medicine for gumtissues and a oral cavity inflammation and consumer product has been under appreciated today since1998. Now days, as the technology develops, improved levels of consumer demand for consumptionand they want the product easier to use. In this study, sustainable refers to both the technology andstandardization characteristics of gel medicine for a new Dentamon or Dentos gels were prepared using20% ethanol extract for mixture of Chamaenerion angustifolium L, Stellera chamajasme L and Oxytropispseudoglandulosa which are pharmacological active for gum tissues and a oral cavity inflammation.GoalThe aim of this work was to standardize of Dentamon elixir gel medicine and make technological studyof Dentamon.Materials and MethodsThe present study included plant species which were Chamaenerion angustifolium L, Stellerachamajasme L and Oxytropis pseudoglandulosa. Those three medicinal plants were collected fromdifferent regions of Mongolia and samples their upper part of ground. The plants were used for thepurpose of their phytochemical analysis and technological study of gel formulation. For the contentof flavonoids, total coumarin and tannin in the gel and extract of those plants were determined byspectrophotometric method. The direct measurement of the microbiological climacteric was determinedin extract by according to Mongolian National Pharmacopeia and the viscosity property of gel medicinewas identified using viscometer.ResultsThis study has revealed the presence of photochemical considered as active medicinal chemicalconstituents. Chemical tests of the screening and identification of main active components in the plantsunder study were carried out in the ethanol extract (20, 40, 70%) and aqueous extract using generalextraction method. The tannin content of the upper part in water and three different concentrated ethanolextract was found to be (2.16±0.04%, 1.73±0.04%, 2.58±0.04% and 1.74±0.02%), respectively. Thetannin content of upper part in 40% of ethanol extract of the plants was 7.40±0.21% and coumarin contentwas 3.01+0.09% and the total flavanoids content were 0.70+0.03%. There were not detected Esherichiacoli, Salmonella, Pseudomonas aeruginosa and Staphylococcus aureus in plant extracts. The gelmedicine was prepared from concentrated plant extract using dispersion method and and gel formingmaterial selection using 0.5%, 1%, 1.5% and 2% of carbomer. The results from gel formulation assay,the 0.5% of the gel was turbid liquid state, and 1% of the gel was a colorless, clear liquid state, 1.5% gelwas colorless, created very clear and 2% gel was colorless but it was very dense. The pH condition ofthe 1% of Dentos gel was 7.6 and the viscosity property was 7400000 mPa/sec, the flavonoid contentwas 0.165%, the total coumarin content was 0.69 and Pseudomonas aeruginosa, Staphylococcusaureus, Enterobacteriaceae did not detected. Dentos 1% gel was compared its pharmacological trialwith Hi Ora gel which is produced by Himalaya LLC. On the treatment 14 days, Dentos gel more reduced45.9% of wound area index than Hi Ora gel.ConclusionThe 40% ethanolic extracts of the studied plants contained many bioactive chemical constituentsincluding alkoloids, flavonoids, tannin and coumarin. The 1.5% of carbomer was most effectivefor make a new Dentos gel and also new generated gel was most effective against Pseudomonasaeruginosa, Staphylococcus aureus, Enterobacteriaceae. The new generated gel was standardizedby its appearance, viscosity property and content of coumarin, alkaliod, flavonoids and microbiologicalpurity characteristics.

Study of secondary plant products is important for understanding their functions and for obtaining biologically active compounds for practical use in different spheres of human activity. Among other plant substances, phytoecdysteroids attract great scientific interest, because of their potential contribution to crop protection strategies and their possible medical uses. Silene repens is widespread in Mongolia. Seed of Silene repens were collected in 2008 from Mongolian and Russian Altai and were cultivated in Siberian Botanical Garden at Tomsk State University, Russia.GoalStudy the dynamics of the 20-hydroxyecdysone in various plant organs of cultivated Silene repens.Material and MethodsThe seasonal dynamics of phytoecdysteroid content (20-hydroxyecdysone) observed in the vegetative and generative organs of cultivated Silene repens was investigated. The quantity of phytoecdysteroids was determined by spectrophotometer UV-1800 (Shimadzu, Japan).ResultsThe content of 20-hydroxyecdysone varied from 0.001 to 1.07 % depending on the organ and growth stage. The maximum content of 20-hydroxyecdysone was observed in actively developing parts of organs: during beginning vegetation in roots (0.3 %); during budding, in the bud (1.00 %); during flowering, in the flowers (1.07 %); during fruiting, in roots (0.31 %). The general trends seen in the distribution of 20-hydroxyecdysone found for cultivated Silene repens from Mongolian and Russian Altai during the plant growth cycle. A high content of 20-hydroxyecdysone during flowering and a low amount during fruiting.ConclusionA high content of 20-hydroxyecdysone during flowering in the flowers and buds (1.07 -1,00 %) and low content of 20-hydroxyecdysone during fruiting in the stem (0.001-0.12 %). The seasonal dynamics of phytoecdysteroids in cultivated Silene repens has been studied for the first time.

Introduction: Calendula officinalis L. is aromatic herbaceous yearling of the family of Asteraceae. Ethanol extract, decoction and ointment of the plant is used to treat or relieve injury, trauma, erosion, purulent trauma or slow healing abrasions, furuncle, carbuncle, congelation,burn, bed sore, herpes and lichen as cream and spray. These pharmacological functions are related to carotinoid, stearin acid, triterpinoid, phenolic compound, polysaccharide, β-carotene, tannin, flavonoids found in flower of calendula officinalis [2, 3].Goal: To define biologically active substances in cultivated Calendula officinalisMaterials and Methods: Calendula officinalis has been harvested from Monos pharmacological institute, garden of medical plants and prepared according to the appropriate standards. β –carotene and flavonoids were quantified by spectrophotometer, Alkaloid, tannin and ascorbic acids were quantified by tetrameter, Extractive substances, ash and humidity were quantified by weight analysis[1]Results: Quantitative analysis of the flower of calendula officinalis has been carried out following first Mongolian national pharmacopeia and Russian National pharmacopeia XI and defined that β –carotene 1.4313%, alkaloids 0.1229%, flavonoids 2.8817%, tannin 1.2376%, ascorbic acid 0.0702%, extractive substances 40.18%, ash content 11.75% and humidity 5.95%. Flower of calendula officinalis has been extracted by water, 30%, 50% and 80% ethanol, then made comparative analysis on content of β–carotiene. When extracted by 80% ethanol, content of β – carotene was the highest or 150 mg. Therefore optimum extraction solvent quantity has been defined to be 80% ethanol.Microbiological analysis has not revealed anyorganisms and bacteria in solid extract of the plant.Conclusions:1) Quality and countable analysis of biological active substance in the flower of calendula officinalis has been completed.2) β –carotene the main active substance in cultivated calendula officinalis, is found to be 1.4 gr which that meets Mongolian National Standards of medicine.3) The 80% ethanol extract of calendula officinalis contained 150mg β –carotene, the maximum content of β –carotene. Hence optimum extraction solvent was found to be 80%ethanol and it will be and used for future research.4) Microbiological parameters of 80% solid extract of the plant has met quality requirements.References:1. государственная Фармакопея XI вûпуск 2. москва 1990. с.1462. Коновалова о.в. рûбалко К.с. БиологическиАêтивные веùества Calendula officinalis L.“растительнûе ресурсû”. 1990. т. 26. вûп.3.с.448-4633. Cetkovic G.S. Djilas S.M. Canadanovic-Brunet J.M. Tumbas V.T. Antioxidant properties of marigold extract. “Food research International”. 2004. V.37. p643-650