Background: Research on lactic acid bacteria has confirmed how specific strains possess probiotic properties and impart unique sensory characteristics to food products. The use of probiotic lactic acid bacteria in many food products, thus confers various health benefits to humans when they are frequently consumed in adequate amounts. Aim: To determine the effect of lactic acid bacteria on the growth of tomatoes. Materials and Methods: The lactic acid bacteria were cultured using the Lactobacillus medium from whipping cream and Dandelion (Taraxacum mongolicum) and identified using the MALDI-TOF MS automated microbial identification analyzer. A solution was prepared using Lactobacillus delbrueckii isolated from whipping cream and Lactobacillus gasseri isolated from Dandelion (10^7CFU/ml), and sterilized tomato seeds were watered for 10 days with the solution, while sterilized distilled water was used as a control. The germination rate of the seeds and the root length were measured and recorded every day. Results: The solution of L.delbrueckii bacteria isolated from cream germinated 100% of the seeds, which is 4% higher than the control seeds, while the solution of L.gasseri bacteria isolated from Dandelion germinated 100%, supporting 4% higher than the control seeds. Seedlings irrigated with the L.delbrueckii bacterial solution exhibited an average length of 10.3cm, which was 1.3cm longer than the control (P=0.003), indicating a statistically significant difference. Similarly, those treated with the L.gasseri solution had an average length of 11.5cm, 2.5cm longer than the control (P=0.005), also demonstrating statistical significance. Conclusion The application of the lactic acid bacterial solution significantly enhanced both the germination of tomato seeds and the growth of the plants compared to the control solution.

Background: This study explores the potential of food industry by-products, such as plant peels, stems, and slags, as valuable sources of lignocellulosic material (LCM), which contains 25-40% xylan. These underutilized resources, often discarded as waste, hold the promise of sustainable applications in biotechnology. By safely extracting xylooligosaccharides (XOS) from LCM biomass, the value of these materials can be significantly enhanced, contributing to green production and supporting sustainable development. XOS, recognized for its prebiotic activity, has been shown to promote the growth of beneficial gut bacteria, making it a vital research area in the fields of food science, medicine, and technology. Aim: To extract and characterize oligosaccharides derived from by-products of the food industry, evaluate their physicochemical properties, and investigate selected biological activities. Materials and Methods: This study utilized microwave pretreatment and enzymatic hydrolysis to isolate and purify XOS from wheat bran and brewers’ spent grains (BSG), provided by Altan Taria LLC and APU CoL, respectively. Microwave irradiation at 200°C for 5 minutes was employed as a pretreatment step, followed by hydrolysis using commercial xylanase (Thermomyces lanuginosus, recombinant Aspergillus oryzae, 2500 BXU/g) at 55°C for 24 hours. The resulting hydrolysate underwent filtration with activated carbon and ethanol precipitation to yield purified XOS. Analytical methods, including FTIR spectroscopy, TLC and HPLC, were used for structural and compositional analysis of the purified oligosaccharides. In vitro tests evaluated the ability of XOS to support the growth of beneficial gut bacteria, including Bifidobacterium spp., Lactobacillus fermentum (ATCC 9338), and Lactobacillus casei (ATCC 344), using XOS-enriched media. Additionally, in vivo studies were conducted on rats to determine the biological effects of XOS on gut microbiota. Results: The results demonstrated that prolonged enzymatic hydrolysis for more than 10 hours, using 0.25 g of xylanase per 100 g of substrate, resulted in optimal yields. XOS purity was measured at 87.6% with an 8.1 g yield from wheat bran and 89% purity with a 7.2 g yield from brewers’ spent grains. Structural analysis confirmed the presence of xylobiose, xylotriose, and xylotetraose, with xylotetraose being the most abundant component in WBP-XOS (47.5%), and xylobiose dominating BGS’s derived XOS (47.8%). Biological effects revealed that wheat bran-derived XOS significantly supported the growth of Bifidobacterium spp. and L. fermentum (ATCC 9338) in a concentration-dependent manner, whereas no significant effect was observed on L. casei (ATCC 344). In vivo studies confirmed that XOS consumption increased populations of Bifidobacterium spp. and Akkermansia muciniphila spp. in gut microbiota (p<0.05). Furthermore, XOS consumption reduced plasma cholesterol, triglycerides, and LDL-C levels while increasing HDL-C levels, demonstrating metabolic benefits. Conclusion This research establishes that XOS with prebiotic activity can be efficiently extracted and purified from food industry by-products using microwave-assisted enzymatic hydrolysis. This approach highlights the potential of utilizing agricultural and industrial waste for producing functional prebiotics, contributing to sustainable practices and offering valuable applications in health and nutrition.

Background: However kidney transplantation has being performed in Mongolia since 2006, because of pre-transplant sensitization, ABO incompatibility, hepatitis B and C virus activation many patients are taken kidney transplantation in abroad. The transplantation centers use own immunosuppressive regimens. Objective: Our aim was to assess the immunosuppressive regimens efficacy and toxicity in kidney transplant Mongolian recipients. Methods: We analyzed data from 96 adult kidney transplant recipients who had taken kidney transplantation in different transplant centers from August 2006 through January 2014. There were 3 kinds of regimens Group I Simulect induction with standard triple /FK506/CyA+MMF/AZA+steroid/, Group II Campath-1H induction with CNI monotherapy and Group III Campath-1H induction with standard triple /FK506/CyA+MMF/AZA+steroid/. We retrospectively collected the post-transplant first two years serum creatinine. The study was performed in 2014. The questionnaire was taken and blood samples collected for determination of tacrolimus through level and for other laboratory tests. The primary end point was the first two years serum creatinine, the secondary end points included rejection episodes, blood through level of tacrolimus and some laboratory findings. Results: The post-transplant first two years serum creatinine levels were significantly different in 3 groups. Group III showed similar results compared to Group I. There was not enough data of biopsy proven acute rejection episodes however group II said more rejections occurred. However participants said that rejection occurred in 15 (15.6%) biopsy was done only 3 (3.1%) cases. Blood through level of tacrolimus was significantly different in three groups. Some laboratory findings showed different between three groups. Conclusions A regimen of Campath-1H induction with CNI monotherapy (Group II) may be advantageous for short-term renal function and cost effective but there were more rejection complications and increased creatinine. The regimen of Campath-1H induction 11 with standard triple (Group III) may be advantageous for long-term renal function, allograft survival, but there should consider about infection complications and polycythemia. Simulect induction with standard triple could be best choice but transplantations were performed in experienced centers. The study enrolled few cases and cases which were performed at the beginning of transplant program so many things could influence on the result. The study was compared beginner transplant center with experienced centers. Longitudinal cohort study needed in the future.

BACKGROUND OF STUDY: According to WHO report estimation, 76.3 million people have been diagnosed with disorder related to alcohol use which has been root cause for over 60 types of disease in the world.1 Smoking causes death to4 million people every year, 11 people every day and 6 every minute. This number is estimated to double reaching 10 million by 2030.2 The study conducted by the ‘World Vision Mongolia’ international organization and the Anti-alcoholism and Drug Association of the Ministry of Justice show 76% of all teenagers has drunk alcohol to some extent. AIMS: To determine alcohol, tobacco and psychoactive drug use by school teenagers of Sukhbaatar district in Ulaanbaatar MATERIAL AND METHODOLOGY: The survey on inquiry of alcohol, tobacco and psychoactive drug use (ASSIST) was conducted among a total of randomly selected 800 (374 boys, 426 girls) students of 8-12th grade from 7 secondary schools of Sukhbaatar district. Statistical data was put into Microsoft office applications (excel, word) and processed by SPSS application with 95% of probability of information and 5.0% of trust level. RESULT OF STUDY: Out of all respondents 32% (256) use tobacco, 43.8% (350)alcoholic drinks, 2.6% (21)cannabis,1.1% (9) cocaine, 1.5% (12) amphetamine, 3%(24)volatile substance, 4.3% (34)sedative and tranquilizer drug, 0.6% (5)hallucinogenic drug, 0.5% (4) opioid, and 0.1% (1) uses other types of drug. Among the survey participants, there are a total of 214 teenagers (in duplicated number) who need short-term action and 2 teenagers who are smoker and need intensive treatment. CONCLUSION: Among total teenagers, the survey participants, 1 in 3 used tobacco, 1 in 2 alcoholic drinks and 1 in 7 used psychoactive drug. Male students smoke twice more than female students and there is no difference in use of alcoholic drink in both sexes. The alcohol and tobacco use among teenagers at the age of 12-18 who participated in the survey showed that they use more when they grow older. 1 in 4 students of all participants belongs to a group with average risk and needs intensive treatment.

Introduction: In 1814 Irish surgeon Abraham Coll first introduced distal radial bone fracture in clinical practice as a colles fracture. It is one of the most common fractures account for 10-20% of the total respectively fracture. Case of Colles fracture has being increased in the developed country year by year besides the increasing number of elderly patients. Depending on severity displaced of the fracture, management includes closed reduction or surgical procedure. The aim of study was to study result of risk factors that influence the closed reduction management of the Colles fracture. Materials and Methods: From hospital based population 80 patients aged between 5-76 years (mean age 47.31 years, male 61.25%, female 38.7%) were recruited by cross sectional and randomized method. Participants were divided into displaced and non-displaced groups which confirmed by refractive index difference on X-ray. Results: The risk factors that influence the colles fracture closed reduction management was osteoporosis (p=0.38), menopause (r=0.18, p=0.27), calcium supplement intake (r=0.21, p=0.05), received hospital care in 24 hour (p=0.39), apply plaster (p=0.64), hand sling immobilizer brace (p=0.5) and physical therapy (p=0.5). Conclusion: Osteoporosis and menopause were the risk factors that influenced the closed reduction management of Colles fracture. The patient cases that not receiving emergency medical care in first 24 hours, not applying plaster, not using the hand sling immobilizer brace and not receiving physical therapy was risk factors for extending the closed reduction management of the Colles fracture.

Kidney transplantation is the best alternative treatment for end-stage renal disease and health-related quality of life and survival of the patients are improved compared with dialysis. Worldwide, more than 1.4 million patients with CKD receive renal replacement therapy with incidence growing by approximately 8% annually.1 Unfortunately, despite significant improvement in graft function, kidney transplants can still fail due to acute rejection and chronic allograft nephropathy.2 Kidney biopsy after transplantation, which has evaluated by Banff 09 classification is usefull method for diagnose of transplanted kidney disease.3,4Kidney graft rejection was diagnosed in 10 renal allograft biopsy specimens (bs) obtained from transplant patients followed up at our institute between 2015 and 2016. All specimens were evaluated as satisfactory which show more than 8 glomerulus under the light microscopy. Each renal cortical tissue was divided into two tips: one piece for routine H&E stain and special stains, including Masson’s trichrome, and PAS stain; another piece for immunofluorescence by frozen section, which were stained with IgA, IgM, IgG and complement component (C3, C4, C1q, C4d). All the renal biopsies were examined by the same pathologist.Out of 117 transplantations, 10 episodes of rejection selected. Among the 10 patients, 30% had an acute T cell rejection and 70% had a chronic allograft nephropathy. Interstitial inflammation (i1-7) was present in 7 bs (70%), tubulitis (t1-4,t2-2) in 6 bs (60%), transplant glomerulitis (g1-1, g2-2, g3-1) in 4 bs (40%), transplant interstitial fibrosis (ci1-2, ci2-2, ci3-2) in 6 bs (60%), tubular atrophy (ct1-6, ct2-2, ct3-1) in 9 bs (90%), mesangial matrix increase (mm1-5) in 5 bs (50%), vascular fibrosis intimal thickeness (cv1-3) in 3 bs (30%), arteriolar hyaline thickening (ah1-5) in 5 bs (50%), tubulitis (ti1-6, ti2-3, ti3-1) in 10 bs (100%) and peritubular capillaritis (ptc1-1, ptc2-2, ptc3-1) in 4 bs (40%). C4d deposition was present very mild in wall of the vessels and peritubular capillaries. Because of not good working Methenamin silver stain, we couldn’t demostrate glomerular basement membrane changes (cg) fully.We suggest that histopathological changes of transplant glomerulopathy might be accompanied by inflammation of the microvasculature, such as transplant glomerulitis and peritubular capillaritis. C4d deposition in the wall of the vessels and peritubular capillaritis is not always present in biopsy specimens of transplant glomerulopathy.

Introduction: In 1814 Irish surgeonAbraham Coll first introduced distal radialbone fracture in clinical practice as a collesfracture. It is one of the most commonfractures account for 10-20% of the totalrespectively fracture. Case of Colles fracturehas being increased in the developed countryyear by year besides the increasing numberof elderly patients. Depending on severitydisplaced of the fracture, managementincludes closed reduction or surgicalprocedure. The aim of study was to studyresult of risk factors that influence theclosed reduction management of the Collesfracture.Materials and Methods: From hospitalbased population 80 patients aged between5-76 years (mean age 47.31 years, male61.25%, female 38.7%) were recruited bycross sectional and randomized method.Participants were divided into displaced andnon-displaced groups which confirmed byrefractive index difference on X-ray.Results: The risk factors that influence thecolles fracture closed reduction managementwas osteoporosis (p=0.38), menopause(r=0.18, p=0.27), calcium supplement intake(r=0.21, p=0.05), received hospital care in24 hour (p=0.39), apply plaster (p=0.64),hand sling immobilizer brace (p=0.5) andphysical therapy (p=0.5).Conclusion: Osteoporosis and menopausewere the risk factors that influenced theclosed reduction management of Collesfracture. The patient cases that not receivingemergency medical care in first 24 hours,not applying plaster, not using the hand slingimmobilizer brace and not receiving physicaltherapy was risk factors for extending theclosed reduction management of the Collesfracture.

BackgroundMonos Pharm LLC has been started production of Dentamon which is an elixir medicine for gumtissues and a oral cavity inflammation and consumer product has been under appreciated today since1998. Now days, as the technology develops, improved levels of consumer demand for consumptionand they want the product easier to use. In this study, sustainable refers to both the technology andstandardization characteristics of gel medicine for a new Dentamon or Dentos gels were prepared using20% ethanol extract for mixture of Chamaenerion angustifolium L, Stellera chamajasme L and Oxytropispseudoglandulosa which are pharmacological active for gum tissues and a oral cavity inflammation.GoalThe aim of this work was to standardize of Dentamon elixir gel medicine and make technological studyof Dentamon.Materials and MethodsThe present study included plant species which were Chamaenerion angustifolium L, Stellerachamajasme L and Oxytropis pseudoglandulosa. Those three medicinal plants were collected fromdifferent regions of Mongolia and samples their upper part of ground. The plants were used for thepurpose of their phytochemical analysis and technological study of gel formulation. For the contentof flavonoids, total coumarin and tannin in the gel and extract of those plants were determined byspectrophotometric method. The direct measurement of the microbiological climacteric was determinedin extract by according to Mongolian National Pharmacopeia and the viscosity property of gel medicinewas identified using viscometer.ResultsThis study has revealed the presence of photochemical considered as active medicinal chemicalconstituents. Chemical tests of the screening and identification of main active components in the plantsunder study were carried out in the ethanol extract (20, 40, 70%) and aqueous extract using generalextraction method. The tannin content of the upper part in water and three different concentrated ethanolextract was found to be (2.16±0.04%, 1.73±0.04%, 2.58±0.04% and 1.74±0.02%), respectively. Thetannin content of upper part in 40% of ethanol extract of the plants was 7.40±0.21% and coumarin contentwas 3.01+0.09% and the total flavanoids content were 0.70+0.03%. There were not detected Esherichiacoli, Salmonella, Pseudomonas aeruginosa and Staphylococcus aureus in plant extracts. The gelmedicine was prepared from concentrated plant extract using dispersion method and and gel formingmaterial selection using 0.5%, 1%, 1.5% and 2% of carbomer. The results from gel formulation assay,the 0.5% of the gel was turbid liquid state, and 1% of the gel was a colorless, clear liquid state, 1.5% gelwas colorless, created very clear and 2% gel was colorless but it was very dense. The pH condition ofthe 1% of Dentos gel was 7.6 and the viscosity property was 7400000 mPa/sec, the flavonoid contentwas 0.165%, the total coumarin content was 0.69 and Pseudomonas aeruginosa, Staphylococcusaureus, Enterobacteriaceae did not detected. Dentos 1% gel was compared its pharmacological trialwith Hi Ora gel which is produced by Himalaya LLC. On the treatment 14 days, Dentos gel more reduced45.9% of wound area index than Hi Ora gel.ConclusionThe 40% ethanolic extracts of the studied plants contained many bioactive chemical constituentsincluding alkoloids, flavonoids, tannin and coumarin. The 1.5% of carbomer was most effectivefor make a new Dentos gel and also new generated gel was most effective against Pseudomonasaeruginosa, Staphylococcus aureus, Enterobacteriaceae. The new generated gel was standardizedby its appearance, viscosity property and content of coumarin, alkaliod, flavonoids and microbiologicalpurity characteristics.

Introduction: Calendula officinalis L. is aromatic herbaceous yearling of the family of Asteraceae. Ethanol extract, decoction and ointment of the plant is used to treat or relieve injury, trauma, erosion, purulent trauma or slow healing abrasions, furuncle, carbuncle, congelation,burn, bed sore, herpes and lichen as cream and spray. These pharmacological functions are related to carotinoid, stearin acid, triterpinoid, phenolic compound, polysaccharide, β-carotene, tannin, flavonoids found in flower of calendula officinalis [2, 3].Goal: To define biologically active substances in cultivated Calendula officinalisMaterials and Methods: Calendula officinalis has been harvested from Monos pharmacological institute, garden of medical plants and prepared according to the appropriate standards. β –carotene and flavonoids were quantified by spectrophotometer, Alkaloid, tannin and ascorbic acids were quantified by tetrameter, Extractive substances, ash and humidity were quantified by weight analysis[1]Results: Quantitative analysis of the flower of calendula officinalis has been carried out following first Mongolian national pharmacopeia and Russian National pharmacopeia XI and defined that β –carotene 1.4313%, alkaloids 0.1229%, flavonoids 2.8817%, tannin 1.2376%, ascorbic acid 0.0702%, extractive substances 40.18%, ash content 11.75% and humidity 5.95%. Flower of calendula officinalis has been extracted by water, 30%, 50% and 80% ethanol, then made comparative analysis on content of β–carotiene. When extracted by 80% ethanol, content of β – carotene was the highest or 150 mg. Therefore optimum extraction solvent quantity has been defined to be 80% ethanol.Microbiological analysis has not revealed anyorganisms and bacteria in solid extract of the plant.Conclusions:1) Quality and countable analysis of biological active substance in the flower of calendula officinalis has been completed.2) β –carotene the main active substance in cultivated calendula officinalis, is found to be 1.4 gr which that meets Mongolian National Standards of medicine.3) The 80% ethanol extract of calendula officinalis contained 150mg β –carotene, the maximum content of β –carotene. Hence optimum extraction solvent was found to be 80%ethanol and it will be and used for future research.4) Microbiological parameters of 80% solid extract of the plant has met quality requirements.References:1. государственная Фармакопея XI вûпуск 2. москва 1990. с.1462. Коновалова о.в. рûбалко К.с. БиологическиАêтивные веùества Calendula officinalis L.“растительнûе ресурсû”. 1990. т. 26. вûп.3.с.448-4633. Cetkovic G.S. Djilas S.M. Canadanovic-Brunet J.M. Tumbas V.T. Antioxidant properties of marigold extract. “Food research International”. 2004. V.37. p643-650

Background: 23 percent of overall 660 graduates after 8 times graduation of Monos university is now employed by Monos Group. One third of the graduates is employed at affiliates and subsidiaries of Monos group and comments and opinions of those graduates will make a considerable contribution in future development of the university curriculum, syllabus and improvement of teaching methodology.Purpose of the study: To research satisfactory indexes of graduatesResearch tools and methods: This research was conducted by means of oral interview and written questionnaire among graduates of Monos university who are currently employed at subsidiaries of Monos group using Excel and Word programs. Questionnaire was filled online by the graduates using Outlook and Mass mail.Research result: The research covered 43 of 134 graduates (35%) of the Monos university employed at Monos group subsidiaries and average age of the participants was 20-45, professions of the participants varied from pharmacist, department heads, chiefs and teachers. Composition and other factors of the participants were able to represent the majority. According to the study, during the study at the university graduates gained sufficient knowledge both in practical and theoretical fields and team work skill (62,5), skill to work one one’s own (68,5%) were the main skills achieved by them. On the other hand, the fact that computer skills 66,6%, skill to work by one’s self /66,3%/, team working skills /63,3%/ werefully achieved at workplace may be affiliated with improvement of those skills after starting employment. Moreover, the graduates replied that they could not fully achieved computer skills during the time of study in the university but developed it later on at workplace and concluded that English language course /68,8%/ and Pharmacology course /50%/ could not provide them with sufficient knowledge required. Therefore, there is a necessity to add supplementary classes of pharmacology and improve teaching quality of English and Computer classes. 81% of the participants in the research are interested in taking post-graduation classes of pharmacology and 56.2% wishes to take pharmaceutical department structure and organization.Conclusion: According to the research result, graduates are 100% satisfied with the university they graduated and major they studied. 90 percent of the participants replied that they were able to achieve practical and theoretical knowledge at sufficient level during the time of study. However, 60 percent of the respondents considered that they were not ab;e to achieve English and computer knowledge at satisfactory level.References:1. Medical education journal volume IV МАУБХUB20122. Pharmacy and Pharmacology journal volume I UB20123. Zultsetseg N, advanced level accreditation report of Monos university student department UB 2012