Objective This study aims to investigate the expression of YTH domain N6-methyladenine RNA binding protein 2(YTHDF2)and UBX domain protein 1(UBXN1)in glioma tissue and their prognostic value.Methods A total of 92 glioma cases that underwent surgical treatment in Qingdao Jiaozhou Central Hospital from February 2017 to February 2018 were included.Immunohistochemistry was used to detect YTHDF2 and UBXN1 expression.Spearman rank correlation analysis was conducted.Kaplan-Meier survival curves were plotted to analyze the association between YTHDF2,UBXN1 expression and prognosis in glioma patients.COX analysis was used to determine the prognostic factors affecting glioma patients.Results Compared with adjacent tissues,the positivity rate of YTHDF2(65.22％vs 15.22％)was significantly higher in gliomas,while the positivity rate of UBXN1(26.09％vs 73.91％)was lower,and differences were statistically significant(x2=47.831,42.087,all P<0.05).Spearman rank correlation analysis,showed a negative correlation between YTHDF2 and UBXN1 expression in gliomas(r=-0.712,P<0.05).Compared with tumors diameter<3cm and WHO grades Ⅰ to Ⅱ,YTHDF2(75.47％vs 51.28％,65.22％vs 50.00％)had a higher positivity rate in glioma tissues with tumor diameter≥3cm and WHO grade Ⅲ,while UBXN1(15.09％vs 41.03％,11.11％vs 47.37％)had a lower positivity rate,and differences were statistically significant(x2=5.795,6.609;7.835,15.207,all P<0.05).The five-year overall survival rate of YTHDF2 positive group was lower than that of negative group[28.33％(17/60)vs 62.50％(20/32)],while the five-year overall survival rate of UBXN1 positive group was higher than that of negative group[66.67％(16/24)vs 30.88％(21/68)],and the differences were statistically significant(Log-Rank x2=12.870,7.665,all P<0.05).YTHDF2 positive(HR=2.427,95％CI:1.426～4.569),UBXN1 negative(HR=1.740,95％CI:1.121～2.568),WHO grade Ⅲ(HR=2.671,95％CI:1.160～6.012)and tumor diameter≥3cm(HR=1.628,95％CI:1.017～2.592)were risk factors for poor survival prognosis in glioma patients.Conclusion YTHDF2 increased and UBXN1 decreased in glioma tissues,both of which are related to WHO grading and tumor diameter,and they are independent factors for evaluating the prognosis of glioma patients.

Objective To explore the effect of hospital health management mode in patients with prediabetes.Methods Eighty pre-diabetes patients who were randomly chosen from physical examination people in the physical examination center of our hospital from April 2011 to June 2011 were divided into the observation group and the control group.The observation group received the hospital health management mode of pre diabetes designed by ourselves,and the control group received the traditional management mode.The awareness rate of diabetes in patients with pre-diabetes,the fasting plasma glucose (FPG),2 h postprandial plasma glucose (2 h PG),systolic blood pressure (SBP),diastolic blood pressure (DBP),low-density lipoprotein (LDL),body mass index (BMI) were evaluated at the beginning of the study,six month after the study and one year after the study.The conversion rate of diabetes was compared one year after the study.Results The awareness rate of diabetes in patients with pre-diabetes,the levels of FPG,2 h PG,SBP and DBP were respectively(5.68 ± 0.43) mmol/L,(9.63 ± 0.67) mmol/L,(117.40 ± 7.40) mmHg,(72.90 ± 5.10) mmHg in the observation group,and were (6.84 ± 1.32) mmol/L,(10.75 ±0.91) mmol/L,(125.60 ± 10.20) mmHg,(77.30 ±6.91) mmHg in the control group,and the differences were statistically significant (x2 =19.156,t =5.030 6,2.285 2,3.955 4,2.615 2,respectively; P ＜ 0.05).The levels of LDL and BMI in the observation group showed a downward trend,but no differences were found in two groups (P ＞ 0.05).One year after the study,the levels of FPG,2 h PG,SBP,DBP,LDL and BMI in the observation group were respectively (5.57 ± 0.38) mmol/L,(9.51 ±0.47) mmol/L,(116.23±7.93) mmHg,(70.4±4.3) mmHg,(3.00±0.37) mmol/L and (23.4 ±2.48)kg/m2,and were lower than (6.91 ±1.15) mmol/L,(10.86±3.21) mmol/L,(128.3± 12.14) mmHg,(79.2 ±9.56) mmHg,(3.6 ±0.41) mmol/L and (27 ±5.41) kg/m2 in the control group,and the differences were statistically significant (P ＜0.05) ; the conversion rate of diabetes was 2.7％ (1/37)in the observation group,and was lower than the 15.8％ (6/38) in the control group,and the difference was statistically significant (x2 =2.405 1,P ＜ 0.05).Conclusions The hospital health management mode of pre diabetes designed by ourselves can improve the awareness rate of diabetes in patients with pre-diabetes,decrease the level of FPG,2hPG,SBP,DBP,LDL and BMI,and reduce the conversion rate of diabetes.

Objective　To detect the genotypes and sequences of the exon 1 of human mannose-binding protein (MBP) allele in 5 Chinese nationalities. Methods　 The genotypes of MBP gene of 5 Chinese nationalities were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The exon 1 of the MBP gene of 22 Chinese Hans was analyzed by using ABI 310 genetic analyzer. Results　 The DNA sequences of exon 1 of Chinese MBP gene were acquired. The allele frequencies of the codon 54 of the MBP gene (MBP-54) of 5 Chinese nationalities were 0.181(Hans), 0.128(Uygurs), 0.181(Mongols), 0.179(Tibetans) and 0.181(Yis). The allele distribution for MBP-54 mutation of 5 Chinese nationalities was in good agreement with Hardy-Weinberg equilibrium. Compared with the Hans, Uygurs had a lower MBP-54 mutation rate. There were no differences in the allele frequencies between the chronic hepatitis B patients and health controls in Chinese Hans. The mutations of the codons 52 and 57 were not detected in this study. Conclusion　 A higher prevalence of MBP-54 mutation was found in 5 Chinese nationalities, MBP-54 mutation was not associated with the persistence of hepatitis B.

Objective To evaluate the frequencies and polymorphisms of CCR5-△32,CCR2-641 and SDF1-3'A alleles conferring resistance to HIV-1 infection in Chinese population from Han ethnic origin.Methods This cohort was comprised of 1251 subjects(915 men and 336 women)aged 15 -80 years and none was HIV-1 positive.Genotyping of allelic CCR5-△32,CCR2-641 and SDF1-3' A variants was performed using PCR or PCR/RFLP assay,and further confirmed by direct DNA sequencing.Results Our finding shows that the△32 deletion mutation in the CCR5 gene does occur in this population and can be inherited in a Mendelian fashion in indigenous Han Chinese at a very low frequency of 0.00119(n= 1254).The frequencies of mutant CCR2-641 and SDF1-3'A alleles were 0.20023(n = 1251)and 0.2873(n = 893),in this population,which are higher than those found in American Caucasians.Furthermore the polymorphisms of CCR2-641 and SDF1-3' A alleles in the Han Chinese population were different from those in American Caucasians.Statistical analysis showed that the genotype distribution of CCR5-△32,CCR2-641 and SDF1-3' A alleles was in equilibrium according to the Hardy-Weinberg equation.Conclusion The CCR5-△32 mutation may not be a major resistant factor against HIV-1 infection in indigenous Han Chinese.The significance of higher frequencies of CCR2-641 and SDF1-3' A alleles (0.20023 and 0.2791)in the Han population remains to be clarified in HIV-1-positive carriers and AIDS patients.