Objective: To investigate the current status and influencing factors of emotional intelligence among postgraduates, so as to provide the evidence for promoting the mental health of postgraduates. Methods: The postgraduates from higher education institutions in Guizhou Province were selected using stratified cluster sampling method from November to December 2023. Demographic information such as gender, college, professional discipline and monthly family income were collected using questionnaire surveys. Stress perception was investigated using the Chinese version of the Stress Perception Scale. Emotional intelligence was investigated using the Chinese version of the Emotional Intelligence Scale. The influencing factors for emotional intelligence were analyzed using a multiple linear regression model. Results: Totally 728 valid questionnaires were recovered, with an effective recovery rate of 94.42%. There were 262 males (35.99%) and 466 females (64.01%). The mean score of emotional intelligence was (77.98±15.71) points, the mean score of emotional assessment was (20.57±4.50) points, the mean score of emotional regulation was (18.63±5.22) points, the mean score of emotional using was (18.90±4.87) points, and the mean score of emotional recognition was (19.89±4.73) points. Multiple linear regression analysis revealed that professional discipline (economics and law, β'=0.108), monthly family income (3 000-5 000 yuan, β'=0.091; >5 000 yuan, β'=0.086) and stress perception (β'=-0.515) were associated with emotional intelligence among postgraduates. Conclusion The emotional intelligence among postgraduates was influenced by professional discipline, monthly family income and stress perception.

Reperfusion injury occurs after return of spontaneous circulation (ROSC) in patients with cardiac arrest (CA), which leads to multiple organ dysfunction, called post-cardiac arrest syndrome (PCAS). PCAS is closely related to the prognosis of CA patients, and is an independent risk factor of survival. Integrated traditional Chinese and Western medicine diagnosis and treatment is critical for improving prognosis of PCAS. In order to guide and standardize integrated traditional Chinese and Western medicine diagnosis and treatment in PCAS among clinicians, nurses and research personnel in China, the Emergency Medicine Professional Committee of the Chinese Society of Integrated Chinese and Western Medicine has established an expert group to determine 14 clinical issues related to the diagnosis and treatment of PCAS with integrated traditional Chinese and Western medicine through clinical survey. The working group formulates a search strategy for each clinical issue according to the PICO principle. Chinese and English literature were searched from CNKI, Wanfang, VIP, SinoMed, PubMed, Embase, and Cochrane Library. The grade of recommendations assessment, development and evaluation (GRADE) were used to form the level of evidence and recommendation. When the literature evidence was insufficient, the recommendations and level of recommendation were formed after expert discussion. Combined with the aspects of generalizability, suitability, and resource utilization, the expert consensus developed 28 recommendations around the 14 aspects of three stages of PCAS, including early circulation, respiratory support and reversible cause relief, mid-term neuroprotection, improvement of coagulation, prevention and treatment of infection, kidney and gastrointestinal protection and blood sugar control, post rehabilitation treatment, providing references for the integrated traditional Chinese and Western medicine of the diagnosis and treatment for PCAS.

Dysfunction of the Hippo pathway enables cells to evade contact inhibition and provides advantages for cancerous overgrowth. However, for a significant portion of human cancer, how Hippo signaling is perturbed remains unknown. To answer this question, we performed a genome-wide screening for genes that affect the Hippo pathway in Drosophila and cross-referenced the hit genes with human cancer genome. In our screen, Prosap was identified as a novel regulator of the Hippo pathway that potently affects tissue growth. Interestingly, a mammalian homolog of Prosap, SHANK2, is the most frequently amplified gene on 11q13, a major tumor amplicon in human cancer. Gene amplification profile in this 11q13 amplicon clearly indicates selective pressure for SHANK2 amplification. More importantly, across the human cancer genome, SHANK2 is the most frequently amplified gene that is not located within the Myc amplicon. Further studies in multiple human cell lines confirmed that SHANK2 overexpression causes deregulation of Hippo signaling through competitive binding for a LATS1 activator, and as a potential oncogene, SHANK2 promotes cellular transformation and tumor formation in vivo. In cancer cell lines with deregulated Hippo pathway, depletion of SHANK2 restores Hippo signaling and ceases cellular proliferation. Taken together, these results suggest that SHANK2 is an evolutionarily conserved Hippo pathway regulator, commonly amplified in human cancer and potently promotes cancer. Our study for the first time illustrated oncogenic function of SHANK2, one of the most frequently amplified gene in human cancer. Furthermore, given that in normal adult tissues, SHANK2's expression is largely restricted to the nervous system, SHANK2 may represent an interesting target for anticancer therapy.

Objective? To explore the professional training will and needs of caregivers for the elderly in Shaanxi province and to provide reference for completing the training program for caregivers for the elderly, clarifying the content of training and making relevant policies. Methods? Totally 1 390 caregivers for the elderly from 143 nursing homes in Shaanxi province were selected by convenient sampling from January to September 2017 and investigated with the self-made questionnaire for their will and needs of professional training. χ2 test was used to identify the influencing factors to the caregivers' will of professional training, and the statistically significant variables were included into the Logistic regression model to analyze the influencing factors to professional training of caregivers for the elderly in Shaanxi province. Results?(1) Professional training will:1 085 caregivers (78.1%) were willing to participate in professional training, while 305 caregivers (21.9%) were not willing to do so. Logistic regression analysis showed that educational background, marital status, needs for professional knowledge, certificate of caregivers, effects of previous training and economic income were the influencing factors to the professional training will of caregivers for the elderly in Shaanxi province (P＜0.05). (2) Professional training needs: the form of training was mainly short-term training (48.4%), and the cost of training was mostly free of charge or borne by their institutions (68.7%). Their desired form of training was graded training (64.3%), and the content of training focused primarily on daily life care (83.7%) and basic care (82.2%). Conclusions? The proportion of caregivers for the elderly in Shaanxi province who are willing to receive professional training is high. Targeted graded professional training should be provided according to different situations of caregivers so as to improve the service of care for the elderly. The government or nursing homes are advised to provide part-time and short-term professional training which focuses primarily on daily life care and psychological care, complete training plans and meet social needs, without increasing economic burden on caregivers for the elderly.

Objective? To analyze the demands of caregivers for layered training content in care institutions for the aged in Shanxi so as to provide a basis for the layered training for caregivers in care institutions for the aged. Methods? From January to September 2017, we selected 1 390 caregivers of care institutions for the aged in Shaanxi province as subjects by convenience sampling. The self-designed questionnaire was used to investigate the demands of caregivers with different characteristics and training experience for layered training content. Results? There were statistical differences in the demands of caregivers with different ages and education levels for training content including life care, humanistic care, laws and regulations, emergency care, psychological care and nursing management (P＜0.05). There were statistical differences in the demands of caregivers with different incomes and certificates for training content including primary care, humanistic care, laws and regulations, emergency care and psychological care (P＜0.05). There were also statistical differences in the demands of caregivers with different training experience for training content including life care, humanistic care, laws and regulations, emergency care, psychological care and nursing management (P＜0.05). Conclusions? Care institutions for the aged should provide the targeted layered training for caregivers based on their education levels, working lives, incomes, certificates and training experience to promote the development of caregivers for the aged.

Objective:To explore the clinical characteristics,diagnosis,and treatments of adrenal insufficiency (AI) in the patients with acquired immune deficiency syndrome (AIDS),and to improve the clinician's understanding of the disease and provide evidences for its diagnosis and treatments.Methods:The clinical data of one patient with AIDS and AI were retrospectively analyzed and the diagnosis and treatments were summarized,and the relative literatures were reviewed.Results:The patient was clearly diagnosed as AIDS and AI after relevant examinations.The symptoms such as fatigue,nausea and vomiting of the patient were disappeared,the food intake of the patient was increased,and the electrolyte was normal after some treatments such as hormone replacement therapy (oral prednisone acetate 5.0 mg at 8:00 am.and 2.5 mg at 4:00 pm.) and symptomatic treatments.The patient died of a severe opportunistic infection when followed up for 37 d.Conclusion:The possibility of AI should be taken into account when the patients with AIDS have unexplained symptoms such as anorexia,nausea,weight loss and more characteristic manifestations such as orthostatic hypotension,hyponatremia or hyperkalemia.Early administration of glucocorticoid replacement therapy is feasible and effective.

Objective To evaluate the effect of initiative bladder function training on uroschesis and urinary tract infections after caesarean section. Methods Toally 200 puerperas with caesarean section and indwelling catheter were divided into observation group and control group, 100 cases in each group. The control group was given passive bladder function training, and the observation group was given initiative bladder function training. The two groups were compared in terms of postoperative infections in the urinary tract, time for first micturition, residual urine volume and micturition effect. Result After intervention, the rate of urinary tract infections in the observation group was lower than that of the control one , the time for and effect of first micturition were shorter and better than those of in the control group, the residual urine volume was shorter (P<0.05). Conclusion The initiative bladder function training by exercising abdominal muscle combined with levator ani muscle exercise can effectively promote the recovery of bladder function and reduce uroschesis and the rate of postoperative urinary tract infections.

Objective To investigate the reasonable allocation of geriatric nursing staff resource in socialized aged organization. Methods We investigated whether the specialized nursing was necessary for pension service and how to get the reasonable allocation of geriatric nursing staff by prospective survey design. By convenient sampling method, 260 geriatric nursing staff who came to college for training were surveyed by questionnaire and interview. Totals of 249 partially effective and 146 totally effective data was collected, recorded and analyzed. Results Aged organization demanded for high-level qualified nursing personnel. 69.2%of those nursing staff thought that masters were needed for guidance or leadership. The plan of reasonable allocation of geriatric nursing staff was showed as:①The patient to nurse ratio was 3.7∶1 (1∶0.27), and the ratio of nurse to geriatric nursing staff was 1∶2. 3; ②The ratio of male to female was 1∶2; ③The ratio of professional qualifications junior to medium to senior was 2.6∶1.9∶1. The percentage of associate chief nurses or above was 20. 3%; ④The ratio of educational background technical secondary school diploma to bachelor to master was 15.6∶9.1∶4.3∶1. The percentage of bachelor or above was 31.9%. Conclusions Specialized nursing is necessary for pension service. It is urgent to promote the reasonable allocation of geriatric nursing staff, and this project needs positive concern and support from the whole society.

The 3T3-L1 preadipocytes were used as carriers in the investigation of total extract, n-butanol extract, CB-1 and CB-2 of Coreopsis tinctoria Nutt. on cell proliferation and differentiation. Three groups at different doses were set for each of the four extract regions of C. tinctoria Nutt., respectively. MTT assay was used to detect 3T3-L1cell proliferation by four extract regions of C. tinctoria Nutt. Oil Red O staining was used to analyze the formation and accumulation of cytoplasmic lipid during cell differentiation. The results showed that compared with the control group, there were significant inhibition on cell proliferation when thetotal extract of C. tinctoriaNutt. at 100 μg·mL-1, n-butanol extract at 0.5, 5, and 50 μg·mL-1, CB-1 and CB-2 at 50 μg·mL-1 (P< 0.01). N-butanol extract showed certain dose-dependent manner (r = -0.903). Oil Red O staining showed that compared with the control group, thetotal extract of C. tinctoria Nutt. at 1, 10, 100 μg·mL-1 can obviously inhibit cell differentiation, reduce the formation of cytoplasmic lipid (P< 0.01). N-butanol extract can inhibit cell differentiation in a dose-dependent manner (r= -0.779). CB-1 and CB-2 obviously inhibited cell differentiation at the concentration of 50 μg·mL-1 (P < 0.01). It was concluded that thetotal extract, n-butanol extract, CB-1 and CB-2 of C. tinctoria Nutt. can inhibit the proliferation and differentiation of 3T3-L1 preadipocytes and reduce the formation of cytoplasmic lipid.

Objective To investigate the clinical characteristics in nonalcoholic steatohepatitis (NASH),to provide the basis for the early clinical diagnosis.Methods 200 cases of non-alcoholic fatty liver disease(NAFLD) were divided into NAFL group(115 cases) and NASH group(85 cases).Age,gender,body mass index,blood pressure,clinical symptoms,accompany illnesses,biochemical and image index in the two groups were analyzed and compared retrospectively.Results Asthenia,accounted for 40％,which was the most common gastrointestinal symptom of NASH group.and abdominal distension,anorexia,nausea and vomiting,liver area pain and liver were all involved.There were no significant differences in mainly gastrointestinal symptoms between patients with NASH and NAFL group(P ＞ 0.05),but no symptoms incidence of the two group were higher (44.7％ vs 49.2％ ％ P ＞ 0.05) ;the incidence of obesity,hyperlipidemia,type 2 diabetes,hypertension in NASH group were significantly different compared with NAFL(45.9％ vs 20％,41.2％ vs 22.3％,28.2％ vs 15.6％ respectively P ＜ 0.05) ; In NASH group,BMI,fasting glucose(FPG),2hPPG,serum ferritin,hyaluronic acid,Ⅳ collageninsulin resistance index (HOWA-IR)increased significantly compared with NAFL[(28.68 ± 0.92)vs (22.21 ± 0.43),(9.63 ± 0.64)mmol/L vs (4.92 ± 0.78)mmol/L,(12.96 ±0.28) mmol/L vs (7.04 ±0.13) mmol/L,(243.56 ±7.95) ng/mL vs (140.03 ± 6.80)ng/mL,(130.26 ±9.i6)ng/mL vs (74.85 ±6.54)ng/mL,(130.56 ±8.16)ng/mL vs (72.68 ±7.24) ng/mL,(5.36±0.45) vs (2.63 ±0.12),respectively P＜0.05)].Conclusion Patients with NASH had no obvious gastroenterology symptoms.Obesity,type 2 diabetes,hypertension are more with NASH,and there may be multiple metabolic disorders.