No abstract available.
Foreign Bodies* ; Granuloma, Foreign-Body* ; Mesotherapy*

BACKGROUND: Platelet-rich plasma (PRP) is an autologous concentration of human platelets contained in a small volume of plasma and has recently been shown to accelerate rejuvenate aging skin by various growth factors and cell adhesion molecules. OBJECTIVE: This study was conducted to evaluate the efficacy and safety of intradermal injection of PRP in the human facial rejuvenation. METHODS: This study was a prospective, single-center, single-dose, open-label, non-randomized controlled clinical study. PRP injected to the upper site of this right infra-auricular area and all face. Saline was injected to the left infra-auricular area. Histopathological examinations were performed before PRP treatment, 28 days after the PRP, and saline (control) treatments. RESULTS: Twenty women ranging in age from 40 to 49 years (mean age, 43.65±2.43 years) were enrolled in the study. The mean optical densities (MODs) of collagen in the pre-treatment, control, and PRP-treated area were measured. They were 539±93.2, 787±134.15, 1,019±178, respectively. In the MOD of PRP, 89.05 percent improvement was found when MOD of PRP was compared with MOD of pre-treatment. The mean MOD of collagen fibers was clearly highest on the PRP side (p<0.001). The PRP-to-saline improvement ratio (89.05% to 46.01%) was 1.93:1. No serious side effects were detected. CONCLUSION: PRP increases dermal collagen levels not only by growth factors, but also by skin needling (the mesotherapy technique 'point by point'). PRP application could be considered as an effective (even a single application) and safety procedure for facial skin rejuvenation.
Aging ; Blood Platelets* ; Cell Adhesion Molecules ; Collagen* ; Female ; Humans ; Injections, Intradermal ; Intercellular Signaling Peptides and Proteins ; Mesotherapy ; Plasma ; Platelet-Rich Plasma* ; Prospective Studies* ; Rejuvenation* ; Skin*

No abstract available.
Mesotherapy* ; Mycobacterium*

No abstract available.
Granuloma, Foreign-Body* ; Mesotherapy*

BACKGROUND: The carpal tunnel syndrome (CTS) is the most common cause of severe hand pain. In this study we treated acute pain in CTS patients by means of local intradermal injections of anti-inflammatory drugs (mesotherapy). METHODS: In twenty-five patients (forty-five hands), CTS diagnosis was confirmed by clinical and neurophysiological examination prior to mesotherapy. A mixture containing lidocaine 10 mg, ketoprophen lysine-acetylsalycilate 80 mg, xantinol nicotinate 100 mg, cyanocobalamine 1,000 mcg plus injectable water was used. Sites of injection were three parallel lines above the transverse carpal ligament and two v-shaped lines, one at the base of the thenar eminence, and the other at the base of the hypothenar eminence. RESULTS: The day after the treatment, all but four patients reported a significant reduction in pain and paresthesias. After 12 months, 17 patients had a complete pain relief, eight patients reported recurrence of pain and sensory symptoms and four out of them underwent surgical treatment. CONCLUSIONS: With the obvious limits of a small-size open-label study, our results suggest that mesotherapy can temporary relieve pain and paresthesias in most CTS patients and in some cases its effect seems to be long-lasting. Further controlled studies are needed to confirm our preliminary findings and to compare mesotherapy to conventional approaches for the treatment of CTS.
Acute Pain* ; Carpal Tunnel Syndrome* ; Diagnosis ; Hand ; Humans ; Injections, Intradermal ; Lidocaine ; Ligaments ; Mesotherapy ; Paresthesia ; Recurrence ; Vitamin B 12 ; Water ; Xanthinol Niacinate

BACKGROUND: Microneedles provide a minimally invasive means to transport molecules into the skin. A number of specific strategies have been employed to use microneedles for transdermal delivery. OBJECTIVE: The purpose of this study was to investigate the safety of two new digital microneedle devices (Digital Hand(R) and Digital Pro(R); Bomtech Electronics Co., Ltd., Seoul, Korea) for the perforation of skin in skin-hairless-1 mice. This device replaces conventional needles and is designed specifically for intradermal delivery. METHODS: We used two newly developed digital microneedle devices to perforate the skin of skin-hairless-1 mice. We conducted a comparative study of the two digital microneedle devices and DTS(R) (Disk type-microneedle Therapy System; DTS lab., Seoul, Korea). To evaluate skin stability, we performed visual and dermatoscopic inspections, measurements of transepidermal water loss, and biopsies. RESULTS: The two novel digital microneedle devices did not induce significant abnormalities of the skin on visual or dermatoscopic inspection, regardless of needle size (0.25~2.0 mm). No significant histopathological changes, such as inflammatory cell infiltration, desquamation of the stratum corneum, or disruption of the basal layer, were observed. The digital microneedle devices and microneedle therapy system produced similar results on measures of skin stability. CONCLUSION: These two novel digital microneedle devices are safe transdermal drug delivery systems.
Animals ; Drug Delivery Systems ; Electronics ; Electrons ; Mesotherapy ; Mice ; Mice, Hairless ; Needles ; Pyridines ; Skin ; Thiazoles ; Water Loss, Insensible

BACKGROUND: Autologous platelet-rich plasma has attracted attention in various medical fields recently, including orthopedic, plastic, and dental surgeries and dermatology for its wound healing ability. Further, it has been used clinically in mesotherapy for skin rejuvenation. OBJECTIVE: In this study, the effects of activated platelet-rich plasma (aPRP) and activated platelet-poor plasma (aPPP) have been investigated on the remodelling of the extracellular matrix, a process that requires activation of dermal fibroblasts, which is essential for rejuvenation of aged skin. METHODS: Platelet-rich plasma (PRP) and platelet-poor plasma (PPP) were prepared using a double-spin method and then activated with thrombin and calcium chloride. The proliferative effects of aPRP and aPPP were measured by [3H]thymidine incorporation assay, and their effects on matrix protein synthesis were assessed by quantifying levels of procollagen type I carboxy-terminal peptide (PIP) by enzyme-linked immunosorbent assay (ELISA). The production of collagen and matrix metalloproteinases (MMP) was studied by Western blotting and reverse transcriptase-polymerase chain reaction. RESULTS: Platelet numbers in PRP increased to 9.4-fold over baseline values. aPRP and aPPP both stimulated cell proliferation, with peak proliferation occurring in cells grown in 5% aPRP. Levels of PIP were highest in cells grown in the presence of 5% aPRP. Additionally, aPRP and aPPP increased the expression of type I collagen, MMP-1 protein, and mRNA in human dermal fibroblasts. CONCLUSION: aPRP and aPPP promote tissue remodelling in aged skin and may be used as adjuvant treatment to lasers for skin rejuvenation in cosmetic dermatology.
Aged ; Blood Platelets ; Blotting, Western ; Calcium Chloride ; Cell Proliferation ; Collagen ; Collagen Type I ; Dermatology ; Enzyme-Linked Immunosorbent Assay ; Extracellular Matrix ; Fibroblasts ; Humans ; Matrix Metalloproteinases ; Mesotherapy ; Orthopedics ; Plasma ; Plastics ; Platelet Count ; Platelet-Rich Plasma ; Rejuvenation ; RNA, Messenger ; Skin ; Thrombin ; Wound Healing

Mesotherapy is a widely used technique of intradermal or subcutaneous microinjection of a drug or cocktail of drugs, at sites of the body with medical or aesthetic problems. Rare cutaneous side effects have been previously reported, including allergic reactions to the administered drugs or skin infections. We herein report a case of an immediate adverse reaction following mesotherapy. A 40-year-old woman was referred to our department with pruritic erythematous urticarial plaques at the sites of application of mesotherapy. She had been treated for abdominal liposis with one session of multiple subcutaneous injections of a drug mixture including aminophylline at a local clinic. After clinical recovery, a skin test using the same drugs was performed. A positive intradermal test was found with aminophylline and ethylenediamine that is an ingredient of aminophylline. These results support that the ethylenediamine component of aminophylline is identified as the etiologic agent.
Adult ; Aminophylline ; Ethylenediamines ; Female ; Humans ; Hypersensitivity ; Injections, Subcutaneous ; Intradermal Tests ; Mesotherapy ; Microinjections ; Skin ; Skin Tests ; Urticaria

Recently, mesotherapy is frequently used for body contouring. But, there is a many controversy on the effect of mesotherapy for fat reduction, as well as it's complication tends to look over. So, we let you know the complicated case of the mesotherapy which we experienced. A 28-year-old woman after mesotherapy on her right lateral thigh was admitted to the hospital because of cellulitis and skin necrosis. The patient had a local injection on the both her lateral thigh for body contouring at local hospital. 2 days before hospitalization, we could see infection on her right lateral thigh. The infection finding was swelling, reddish to purple color change and bullae formation. As time goes by, skin necrosis and eschar formation took a place. The size of eschar was estimated about 12 x 10 cm. MRI was accomplished, and the cellulitis on the right hip ant thigh was found on the MRI. The eschar was surgically removed and wound bed was exposed to subcutaneous fatty layer. And then, split thickness skin graft taken from left lateral thigh was done successfully. The grafted skin was well taken without complication, as well as donor site was well healed. The mesotherapy for body contouring is tried by various method, but, the definite mechanism is not established and safe way of drug combination is not clear. For more safer procedure, more study about the way of drug combination and mechanism is needed.
Adult ; Ants ; Blister ; Cellulitis ; Female ; Hip ; Hospitalization ; Humans ; Hypogonadism ; Linear Energy Transfer ; Mesotherapy ; Mitochondrial Diseases ; Necrosis ; Ophthalmoplegia ; Skin ; Thigh ; Tissue Donors ; Transplants

BACKGROUND: Hair loss including androgenetic alopecia and chronic telogen effluvium is recognized increasingly as a physically and psychologically harmful medical condition. Mesotherapy is considered as a new therapeutic modality for hair loss. OBJECTIVE: We studied to determine the effect of medications used in mesotherapy on hair organ culture and culture of dermal papilla cells. METHODS: First, occipital hair follicles were collected from patients with androgentic alopecia and separated into single hair follicles. The single hair follicles were cultured in William E media mixed with mesotherapy medications such as lidocaine, placental extract, Pondil(R), CRP-1000(R), and mixture of all these medications at different concentrations (1, 10, 50 microliter). On the 8th day, the cultured single hairs were stained with H&E and the length of those was measured under a microscope to compare with control group. Immunofluorescent study was performed to check expression of Ki-67, Bcl-2 and Bax on the hairs. Second, dermal papilla cells were isolated from occipital anagen hairs of patients with androgenetic alopecia and cultured in Dulbeco's modified Eagle's medium (DMEM). The mesotherapy medicines were added to the medium with one and two thousand dermal papilla cells, respectively. At the 3rd day, survival of the cells was evaluated with ELISA method comparing with control group. RESULTS: There were no statistical differences of the length of the hairs and the survival of the dermal papilla cells between experimental and control groups. With Bcl-2, we couldn't see any differences between experimental and control groups. With Ki-67, experimental groups showed less expression than control group. On the contrary, experimental groups showed more expression than control group in case of Bax. CONCLUSION: We can conclude from the results that the four medications used in mesotherapy are not effective for growth of cultured hair follicles and survival of cultured dermal papilla cells. However, more study would be needed for the establishment of objective and scientific evidences supporting mesotherapy and we should be in search for new medications for mesotherapy.
Alopecia ; Enzyme-Linked Immunosorbent Assay ; Hair Follicle ; Hair* ; Humans ; Lidocaine ; Mesotherapy* ; Organ Culture Techniques*