【Objective】 To investigate the allele frequencies polymorphic distribution of Duffy, Kidd and Diego blood group systems in Dongxiang ethnic group in Gansu Province. 【Methods】 Blood samples of 100 unrelated blood donors were randomly selected from Dongxiang ethnic group in Gansu from January to December 2017. Allelic typing of Duffy, Kidd and Diego blood groups was performed by fluorescence PCR. 【Results】 The allele frequencies of Duffy, Kidd, and Diego blood group systems of Dongxiang ethnic group were 0.835 for Fy^*01, 0.165 for Fy^*02, 0.570 for Jk^*01, 0.430 for Jk^*02, 0.020 for DI^*01, 0.980 for DI^*02, respectively. No Fy(a-b-), Jk(a-b-), Di(a+b-) rare phenotypes were found. The antigen incompatibility rates of Fy^a/Fy^b, Jk^a/Jk^b, Di^a/Di^b of Duffy, Kidd, and Diego blood group systems were 23.76%, 37.01% and 3.84%, respectively. 【Conclusion】 The allele frequencies distribution of Duffy, Kidd and Diego blood group systems in Dongxiang ethnic group in Gansu were polymorphic and has unique ethnic distribution characteristics.

【Objective】 To investigate the anemia status of infants and toddlers aged 6 - 24 months in Linxia Hui Autonomous Prefecture, Gansu Province, and to comprehensively evaluate the differences in feeding behaviors between anaemic and normal children through the infant and child feeding index (ICFI) and feeding knowledge scores, so as to provide reference for the guidance of infants and young children feeding in ethnic minority areas and the promotion of children′s growth and development. 【Methods】 Taking infants and young children aged 6 - 24 months in Linxia Prefecture as the study subjects, a multi-stage random sampling method was used to select children who met the requirements from 5 townships and 5 villages in 7 counties in 2019 and 2020.Periphral blood samples were collected to test the level of hemoglobin, so as to determine the anemia status.Meanwhile, physical examination was performed and a questionnaire survey of guardians was conducted to analyze the association betweenanaemia and feeding patterns 【Results】 A total of 3 901 infants and children were included in this study, of whom 729 (18.70%) were anaemic, with a mean ICFI score of 12.56±2.70 and a mean feeding knowledge score of 1.97±1.01.There was no statistically significant association of low feeding knowledge score and low ICFI with anaemia after adjusting for confounders (P>0.05), Unqualified meat addition in ICFI was a risk factor for anaemia (OR=1.355, P=0.042), while non-bottle feeding in the past 24 hours (OR=0.762, P=0.021), and breastfeeding in the past 24 hours of infants and toddlers aged 12 - 24 months (OR=0.228, P=0.018) were protective factor for anemia in infants and toddlers aged 12 - 24 months. 【Conclusions】 The average prevalence of anemia in infants and toddlers aged 6 - 24 months in Linxia Hui Autonomous Prefecture of Gansu Province is high, but the level of infant feeding and the level of feeding knowledge of caregivers are low.Early adherence to breastfeeding, timely addition of supplementary food, and more comsumpution of meat for children are conducive to preventing anemia.

OBJECTIVE To study the improvement effects of poria acid on insulin resistance in rats with polycystic ovary syndrome （PCOS） and its mechanism. METHODS One hundred and twenty-six female rats were randomly separated into blank group， PCOS group， poria acid low-dose group （8.33 mg/kg）， pachymic acid high-dose group （33.32 mg/kg）， ethinylestradiol cyproterone group （positive control group， 0.34 mg/kg）， recombinant rat high mobility group protein B1 protein （rHMGB1） group （8 μg/kg）， and poria acid high dose+rHMGB1 group （33.32 mg/kg poria acid+8 μg/kg rHMGB1）， with 18 rats in each group. Except for the blank group， the rats in all other groups were given Letrozole suspension intragastrically to construct the PCOS model. After successful modeling， administration was performed once a day for 4 weeks. After medication， the fasting blood glucose and fasting insulin levels， and insulin resistance index （HOMA-IR） were measured in rats； the levels of follicle-stimulating hormone （FSH）， luteinizing hormone （LH） and testosterone （T） in rat serum， and the levels of interleukin-1β （IL-1β） and tumor necrosis factor- α （TNF- α） in ovarian tissue were detected； ovarian coefficients of rats were calculated； the pathological changes of ovarian tissue were observed； the expressions of HMGB1， receptor for advanced glycosylation elaine_ tanghong@sina.com end product （RAGE） and phosphorylated nuclear factor κB p65 （p-NF-κB p65） proteins were determined in ovarian tissue of rats. RESULTS Compared with the blank group， the pathological injury of ovarian tissue of rats in the PCOS group was serious， the levels of fasting blood glucose and fasting insulin， HOMA-IR and ovarian coefficient were increased， the levels of serum LH and T were increased， while the levels of FSH were decreased； the levels of IL-1β and TNF-α， the expressions of HMGB1， RAGE and p-NF-κB p65 protein in ovarian tissue were increased， with statistical significance （P＜0.05）. Compared with the PCOS group， pathological damage of ovarian tissue was reduced in poria acid low-dose and high-dose groups and ethinylestradiol cyproterone group， and fasting blood glucose， fasting insulin levels， HOMA-IR and ovarian coefficient were decreased； serum LH and T levels were decreased， while FSH levels were increased； the levels of IL-1β and TNF-α and the expressions of HMGB1， RAGE and p-NF-κB p65 protein in ovarian tissue were decreased， with statistical significance （P＜0.05）. The trend of corresponding indexes in rHMGB1 group was opposite to the above （P＜0.05）. Compared with poria acid high-dose group， the changes of the above indexes were reversed significantly in poria acid high-dose+rHMGB1 group （P＜0.05）. CONCLUSIONS Poria acid may improve insulin resistance and inhibit inflammatory reaction in PCOS rats by inhibiting HMGB1/ RAGE pathway.

Objective Visual electrophysiology and optical correlation tomography angiography(OCTA)were used to investigate differences in preoperative retinal function in patients with type 2 diabetic cataract(DC)without obvious retinopathy(NDR)and to determine the clinical application of whole-process blood glucose man-agement(WBGM)for improving postoperative vision in DC patients.Methods This study investigated the preop-erative and postoperative visual electrophysiology(N75,P100,photopic FERG,and scotopic FERG),peripapil-lary retinal nerve fiber layer(pRNFL)and peripapillary capillary vessel density(ppVD)data,as well as trends in these data changes during blood glucose management intervention.Results As the course of T2DM progressed,FBG and HbA1c increased,the N75 and P100 lategraduancy periods of patients gradually lengthened,and the photopic FERG,scotopic FERG,pRNFL,and ppVD values decreased at each postoperative time point.At the same time,the best corrected visual acuity(BCVA)of patients after surgery gradually decreased(P<0.05).Compared with that at 1 week after surgery,the BCVA of Group A(without whole-process blood glucose manage-ment)gradually increased at 1 month and 3 months after surgery but showed a downward trend at 1 year after sur-gery.The BCVA of Group B(with whole-process blood glucose management)gradually stabilized at 1 month after surgery,and at all time points after surgery,the BCVA of Group B was better than that of Group A.The results showed that N75 and P100 in Group A were greater than those in Group B,while the photopic and scotopic FERG,pRNFL,and ppVD(%)in Group A were lower than those in Group B.In addition,N75 and P100 in Group A showed a gradual prolongation trend at various time points after surgery,while photopic FERG,scotopic FERG,pRNFL,and ppVD(%)showed a gradually decreasing trend.However,the above data in Group B gradu-ally stabilized at 3 months after DC surgery,approaching the preoperative level of the group(P<0.05).In the state of whole blood glucose management,although N75 and P100 both reached their longest durations at 1 week af-ter surgery,N75,P100,photopic FERG,scotopic FERG,and pRNFL showed a gradually decreasing trend at 1 month and 3 months after surgery,while ppVD(%)gradually increased(P<0.05).At various time points from 3 months to 1 year after surgery,the overall trend of the above indicators remained stable and close to the preoperative values(P>0.05).Conclusion According to the results of the quantitative analysis of visual electrophysiology and OCTA,in DC patients without obvious retinopathy and with the worsening of diabetes,retinal function decreased,but whole-process blood glucose management effectively restored retinal function and improved visual quality after surgery.

Objective:To investigate the clinical features of chronic spontaneous urticaria (CSU) patients with angioedema (AE) .Methods:Clinical data were collected from adult outpatients with active CSU diagnosed and treated at the First Hospital of China Medical University from January 2019 to December 2021, and analyzed retrospectively. The data included gender, age, disease duration, the presence or absence of angioedema, urticaria activity score for one day, prior treatments, previous history, family history, laboratory test results, therapeutic effect, and adverse reactions. Their treatment regimens were based on the Chinese guidelines for the diagnosis and treatment of urticaria (2018) and the American guidelines for the diagnosis and management of urticaria (2014). Statistical analysis was carried out by using Mann-Whitney U test, two-independent-sample t test, Chi-square test, corrected Chi-square test, and Fisher's exact test. Results:A total of 131 CSU patients were collected, including 78 females and 53 males. Their age at the first visit was 44.6 ± 13.3 years, and the disease duration ( M[ Q1, Q3]) was 4.0 (2.0, 10.0) months. Among these CSU patients, there were 58 with AE and 73 without AE. The disease duration was significantly longer in the CSU patients with AE (6.0 [3.0, 24.0] months) than in those without AE (3.5 [2.0, 6.0] months; Z = -2.78, P = 0.005). The urticaria activity score for one day was also significantly higher in the CSU patients with AE (5.0 [3.0, 5.3] points) than in those without AE (4.0 [3.0, 5.0] points; Z = -2.63, P = 0.008). The CSU patients with AE showed a decreased proportion of patients completely controlled by licensed-dose second-generation H1-antihistamines (sgAHs) (8.6%, 5/58) compared with those without AE (24.7%, 18/73), but an increased proportion of patients uncontrolled by licensed-dose sgAHs (91.4%, 53/58) compared with those without AE (74.0%, 54/73; Z = -2.53, P = 0.011) ; there were no significant differences in the proportions of patients completely controlled or uncontrolled by updosed sgAHs alone or combinations of 2- to 4-fold equivalent-dose sgAHs, or in the proportions of patients completely controlled or uncontrolled by combination therapy with 4-fold equivalent-dose sgAHs and non-H1-antihistamines between the CSU patients with AE and those without AE ( P > 0.05) . Conclusion:Compared with the CSU patients without AE, the CSU patients with AE had a longer disease duration, higher disease activity, a lower proportion of patients completely controlled by licensed-dose sgAHs, and a higher proportion of patients uncontrolled by licensed-dose sgAHs.

The construction of work style is related to the image of the Party and the government,the success or failure of the cause,and is a strategic task of the Party's construction.This article starts from the actual work of public hospital style con-struction,combines responsibility implementation,system construction,especially the practice and effectiveness of project-based management in key areas,reflects the exploration and construction of a long-term mechanism for hospital style construction by Nantong Cancer Hospital in Jiangsu Province,clarifies the target path,and explores a set of supervision work mechanism that is in line with the actual development of public hospitals,To provide strong political support for the hospital's further high-quality development under the current situation.

【Objective】 To investigate the resource allocation status of blood testing laboratories in 14 blood stations in Gansu Province, explore the impact of differences in basic conditions on the comprehensive testing ability of laboratories, so as to promote the homogenization and standardization of blood screening capacity in blood stations in Gansu and improve blood safety and effectivenes. 【Methods】 An evaluation index system of laboratory resource allocation was constructed and a question-naire was designed. The data of human resources, infrastructure and key equipment of 14 blood stations were collected. The entropy weight -TOPSIS method was used to evaluate and rank the resource allocation of 14 blood stations. 【Results】 In the comprehensive evaluation of blood testing laboratory resource allocation in 14 blood stations in Gansu, the top three were laboratories A, B and I, and the last three were laboratories G, M and J. On the whole, the main issue was unreasonable structure of human resources: most laboratories had unreasonable age structure; except for Laboratory A, there was no personnel with bachelor's degree or above in laboratories; most laboratories had not established a team with intermediate professional titles. In terms of infrastructure, the size of seven laboratories could not meet the needs of modern laboratory testing, and all eight blood stations had no spare nucleic acid laboratories nor a mutual spare laboratory with other blood stations As for the key equipment, 5 laboratories had no automatic blood grouping diagnostic instrument, 5 laboratories only had one set of enzyme immunoassay detection system, 3 laboratories had no spare equipment for the key equipment, which means if the equipment failure could not be repaired in time, the release of results would be affected. 【Conclusion】 There were significant differences in human resources, infrastructure and key equipment of blood testing laboratories in 14 blood stations in Gansu, which had a great impact on laboratory testing capacity and subsequent development. It is suggested that governments at all levels and health administrative departments optimize the input of laboratory resource allocation according to the blood collection volume of blood stations to gradually narrow the differences in resource distribution between different regions, improve the degree of laboratory automation and optimize the personnel structure, so as to build high-quality and efficient blood testing laboratories and ensure the safety of clinical blood use.

Objective:To summarize the complications and prevention after botulinum toxin type A(BTA) injection for masseter muscle hypertrophy(MMH).Methods:The clinical data of patients with masseter hypertrophy who received BTA injection in Shanxi Bethune Hospital from January 2019 to September 2021 were retrospectively analyzed. Injection method: 100 U BTA was prepared into a solution with a concentration of 50 U/ml with 2 ml 0.9% sodium chloride injection. The "safe injection zone" of the masseter muscle was marked. The anterior and posterior boundaries were the anterior and posterior edges of the masseter muscle, respectively. The upper boundary was the line from the corner of the mouth to the earlobe, and the lower boundary was the lower edge of the mandible. The injection points(3 or 4 points) were at least 1 cm away from the four borders, and 25-40 U was injected on each side. The patients were followed up after treatment, the types of complications were recorded, and the treatment measures were summarized.Results:A total of 160 patients were enrolled, including 8 males and 152 females, with a mean age of (30.6±6.2) years. All patients were followed up for 1 to 6 months. The masseter muscle of all patients was reduced to varying degrees, the lower facial lines were softer, and the facial contour was improved. The incidence of complications was 81.3%(130/160). There were four main types of complications: (1) Complications with a nonmuscular etiology include ecchymosis in 5 cases (3.1%) which resolved spontaneously in 5 to 7 days and headache occurred in 2 cases (1.3%) which disappeared in 2-3 days with proper rest. (2) The complication related to the toxin effect mainly was the decrease of masticatory power in 86 cases (53.8%), which gradually recovered with the drug metabolism for 3-12 weeks. (3) Dose-related complications include facial ptosis in 4 cases (2.5%) with 30-40 U dose per side. 2 cases without special treatment, gradually recovered in about 8-12 weeks. One patient underwent the BTA injection to raise the mandibular margin and gradually recovered about 2 weeks later. One patient recovered gradually with the treatment by Thermage in another hospital. There were 9 cases (5.6%) of paradoxical rigid masseter muscle bulge. 5 cases resolved within 1 week after supplementary injection of 4 U botulinum toxin, and the other 4 cases resolved spontaneously within 1 to 2 weeks without special treatment. (4) Complications related to the injection site include abnormal smiles in 3 cases (1.9%). Patients were instructed to practice the smile correctly and gradually recovered in 4-12 weeks. Twenty-one patients (13.1%) had sunken cheeks. One patient improved by injection of hyaluronic acid, and the other patients recovered gradually within 8-12 weeks without special treatment.Conclusion:The incidence of complications after BTA injection for MMH is high, and the manifestations are diverse. The injection should be performed in the recommended safe area of the masseter muscle. A reasonable dose and depth of injection can reduce the occurrence of complications.

Objective:To summarize the complications and prevention after botulinum toxin type A(BTA) injection for masseter muscle hypertrophy(MMH).Methods:The clinical data of patients with masseter hypertrophy who received BTA injection in Shanxi Bethune Hospital from January 2019 to September 2021 were retrospectively analyzed. Injection method: 100 U BTA was prepared into a solution with a concentration of 50 U/ml with 2 ml 0.9% sodium chloride injection. The "safe injection zone" of the masseter muscle was marked. The anterior and posterior boundaries were the anterior and posterior edges of the masseter muscle, respectively. The upper boundary was the line from the corner of the mouth to the earlobe, and the lower boundary was the lower edge of the mandible. The injection points(3 or 4 points) were at least 1 cm away from the four borders, and 25-40 U was injected on each side. The patients were followed up after treatment, the types of complications were recorded, and the treatment measures were summarized.Results:A total of 160 patients were enrolled, including 8 males and 152 females, with a mean age of (30.6±6.2) years. All patients were followed up for 1 to 6 months. The masseter muscle of all patients was reduced to varying degrees, the lower facial lines were softer, and the facial contour was improved. The incidence of complications was 81.3%(130/160). There were four main types of complications: (1) Complications with a nonmuscular etiology include ecchymosis in 5 cases (3.1%) which resolved spontaneously in 5 to 7 days and headache occurred in 2 cases (1.3%) which disappeared in 2-3 days with proper rest. (2) The complication related to the toxin effect mainly was the decrease of masticatory power in 86 cases (53.8%), which gradually recovered with the drug metabolism for 3-12 weeks. (3) Dose-related complications include facial ptosis in 4 cases (2.5%) with 30-40 U dose per side. 2 cases without special treatment, gradually recovered in about 8-12 weeks. One patient underwent the BTA injection to raise the mandibular margin and gradually recovered about 2 weeks later. One patient recovered gradually with the treatment by Thermage in another hospital. There were 9 cases (5.6%) of paradoxical rigid masseter muscle bulge. 5 cases resolved within 1 week after supplementary injection of 4 U botulinum toxin, and the other 4 cases resolved spontaneously within 1 to 2 weeks without special treatment. (4) Complications related to the injection site include abnormal smiles in 3 cases (1.9%). Patients were instructed to practice the smile correctly and gradually recovered in 4-12 weeks. Twenty-one patients (13.1%) had sunken cheeks. One patient improved by injection of hyaluronic acid, and the other patients recovered gradually within 8-12 weeks without special treatment.Conclusion:The incidence of complications after BTA injection for MMH is high, and the manifestations are diverse. The injection should be performed in the recommended safe area of the masseter muscle. A reasonable dose and depth of injection can reduce the occurrence of complications.

A 3-year-old girl was hospitalized for cough and asthma for 2 days, and fever and anuria for 1 day in Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology in January 2019.Retrospective analysis was used.She developed end-stage renal disease and received peritoneal dialysis 6 months ago.After admission, she was diagnosed as acute-on-chronic renal failure, respiratory failure, heart failure, severe influenza A (H1N1). The patient was rapidly recovered by the management of high-volume peritoneal dialysis, mechanical ventilation, and medications of Peramivir and Methylprednisolone.Through literature review, case report or cohort study about the treatment of acute kidney disease by high-volume peritoneal dialysis has not been previously reported.This case report suggested that high-volume peritoneal dialysis is able to effectively remove solutes and control volume without causing severe hypoproteinemia and hyperglycemia, which may become an effective renal replacement therapy for children with multi-organ dysfunction syndrome.