Hypoxia-inducible factor (HIF-1α), a core transcription factor responding to changes in cellular oxygen levels, is closely associated with a wide range of physiological and pathological conditions. However, its differential impacts on vascular cell types and molecular programs modulating human vascular homeostasis and regeneration remain largely elusive. Here, we applied CRISPR/Cas9-mediated gene editing of human embryonic stem cells and directed differentiation to generate HIF-1α-deficient human vascular cells including vascular endothelial cells, vascular smooth muscle cells, and mesenchymal stem cells (MSCs), as a platform for discovering cell type-specific hypoxia-induced response mechanisms. Through comparative molecular profiling across cell types under normoxic and hypoxic conditions, we provide insight into the indispensable role of HIF-1α in the promotion of ischemic vascular regeneration. We found human MSCs to be the vascular cell type most susceptible to HIF-1α deficiency, and that transcriptional inactivation of ANKZF1, an effector of HIF-1α, impaired pro-angiogenic processes. Altogether, our findings deepen the understanding of HIF-1α in human angiogenesis and support further explorations of novel therapeutic strategies of vascular regeneration against ischemic damage.
Humans ; Vascular Endothelial Growth Factor A/metabolism* ; Endothelial Cells/metabolism* ; Transcription Factors/metabolism* ; Gene Expression Regulation ; Hypoxia/metabolism* ; Cell Hypoxia/physiology*

Mastering the structure,composition and working principle of digital subtraction angiography(DSA)equipment is an important prerequisite for medical engineers to carry out daily maintenance and repair management.By analyzing the structural composition and functional components of DSA from the aspects of X-ray source,mechanical drive,host control and image output,the working process of subtraction imaging was described according to Beer-Lambert law,and the influencing factors of DSA image quality were summarized.At the same time,taking the Allura XPer FD20 DSA equipment as an example,the failure cases of examination bed,rack and temperature control that occurred in clinical use were analyzed,and the fault investigation and processing were carried out from the hardware structure and circuit module of the equipment,and the equipment fault alarm information was solved in time to ensure the operation quality of the equipment and improve the efficiency of fault maintenance.

Hair loss affects millions of people at some time in their life, and safe and efficient treatments for hair loss are a significant unmet medical need. We report that topical delivery of quercetin (Que) stimulates resting hair follicles to grow with rapid follicular keratinocyte proliferation and replenishes perifollicular microvasculature in mice. We construct dynamic single-cell transcriptome landscape over the course of hair regrowth and find that Que treatment stimulates the differentiation trajectory in the hair follicles and induces an angiogenic signature in dermal endothelial cells by activating HIF-1α in endothelial cells. Skin administration of a HIF-1α agonist partially recapitulates the pro-angiogenesis and hair-growing effects of Que. Together, these findings provide a molecular understanding for the efficacy of Que in hair regrowth, which underscores the translational potential of targeting the hair follicle niche as a strategy for regenerative medicine, and suggest a route of pharmacological intervention that may promote hair regrowth.
Mice ; Animals ; Quercetin/pharmacology* ; Endothelial Cells ; Hair ; Hair Follicle ; Alopecia

Humans ; Mechanistic Target of Rapamycin Complex 2/metabolism* ; Multiprotein Complexes/metabolism* ; Neural Stem Cells/metabolism* ; Proto-Oncogene Proteins c-akt/metabolism* ; Rapamycin-Insensitive Companion of mTOR Protein/metabolism* ; TOR Serine-Threonine Kinases/metabolism*

Gallic Acid/pharmacology* ; Humans ; Senotherapeutics ; Stem Cells

Animals ; Chloroquine/pharmacology* ; Longevity ; Rats

Small cell lung cancer (SCLC) is the most malignant lung cancer with the highest mortality. At present, the first-line standard treatment is still based on Etoposide and Platinum chemotherapy. However, for SCLC that progresses after first-line therapy, the treatment options are still very limited. Since the molecular mechanism of first-line drug resistance of SCLC is still unclear, and the precision medicine strategy after first-line drug resistance is still in the pre-clinical stage. The proportion of secondary biopsy and genetic testing is very low after the progress of first-line treatment of SCLC. In this study, we report a case of a middle-aged woman who was first diagnosed with SCLC. Adenocarcinoma with sensitive gene mutations and repeated changes of small cell carcinoma were detected by multiple biopsies during the course of the disease, suggesting that the patient may be a special subtype of SCLC - mixed SCLC (M-SCLC). In this case, the patient has been treated with radiotherapy and chemotherapy, immunotherapy and targeted therapy successively, and the survival time has reached 2 years and 8 months. Through the case report and literature review retrospectively, this study aimed to explore the part patients may start to present hybrid histopathologic types or tissue type change after treatment of SCLC. Biopsy pathologic histology and genetic testing is necessary after disease progression to look for potential therapeutic targets, so as to give precise treatment based on molecular markers detection results and provide the patient with the benefit of survival for as long as possible.
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Adenocarcinoma of Lung ; Etoposide ; Female ; Humans ; Lung Neoplasms/genetics* ; Middle Aged ; Retrospective Studies ; Small Cell Lung Carcinoma/genetics*

Objective:To explore the effect of Wagner grade home follow-up management of diabetic foot led by specialist nurses.Methods:From January 2017 to December 2018, convenience sampling was used to select 241 patients with type 2 diabetes and Wagner grade 0 diabetic foot in the Endocrinology Department of the First Affiliated Hospital of Xinxiang Medical University. According to the enrollment time, 113 patients with Wagner grade 0 diabetic foot enrolled from January to December 2017 were set as the control group, and 128 patients with Wagner grade 0 diabetic foot enrolled from January to December 2018 were set as the observation group. The control group implemented routine home care management, and the observation group carried out the Wagner grade home follow-up management of diabetic foot led by specialist nurses. Both groups were followed up for 18 months. The blood glucose index, Scale of the Diabetes Self-care Activities (SDSCA) score and foot prognosis were compared between the two groups.Results:After the intervention, the fasting blood glucose and glycosylated hemoglobin of the observation group were lower than those of the control group, and lower than those before the intervention, and the SDSCA score was higher than that of the control group, and higher than that before the intervention, the differences were statistically significant ( P<0.05) . At the end of the sixth month, the observation group had no patients in grade 4 and grade 5, had more people in grade 0 to 1 than in the control group, and fewer people in grade 2 to 3 than in the control group, and the difference was statistically significant ( P<0.05) . At the end of the twelfth and eighteenth months, the observation group had more people in grades 0 to 1 than in the control group, and fewer in grades 2 to 3 and 4 to 5 than in the control group, and the difference was statistically significant ( P<0.05) . At the end of the eighteenth month, there were no deaths in the two groups, and 6 patients with surgery, and 1 patient with amputation in the control group, and 1 patient with surgery and without amputation in the observation group. Conclusions:The Wagner grade home follow-up management of diabetic foot led by specialist nurses can effectively improve the patient's blood glucose control level, self-management ability, and delay the progress of diabetic foot.