Hemophilia B is a genetic disorder caused by coagulation factor Ⅸ(FⅨ) deficiency, mainly manifesting as joint, muscle and deep tissue bleeding. Hemophilia pseudotumor is a mass formed by soft tissue liquefaction and necrosis caused by repeated bleeding. Most pseudotumors occur in the bone and muscle. We report a case of hemophilia B with pseudotumor formation in the pelvis and abdomen, where lesion location is relatively rare. After active and effective hemostasis, the patient's hematuria symptom gradually improved. This case suggests that early and timely hemostatic treatment is crucial for patients with hemophilia.

OBJECTIVES: To investigate the effect of icariin (ICA) on early β-defensin-2 and T cell subsets in rats after tracheotomy. METHODS: A total of 54 SPF male Sprague-Dawley rats were randomly divided into a normal control group (group A), a model group (group B), and a model+ICA treatment group (group C), with 18 rats in each group. A tracheotomy intubation model of the B and C group was prepared. After 6 h of surgery, ICA intervention was given to group C. Groups A and B were given the same amount of normal saline. Lung tissue, alveolar lavage fluid and peripheral blood were taken at 24 h, 72 h and 168 h, respectively. The expression of rat β-defensin-2 mRNA in lung tissue was detected by RT-PCR. The content of β-defensin-2 in alveolar lavage fluid and peripheral blood serum was detected by ELISA. The content of peripheral blood T cell subsets (CD3, CD4, CD8) was detected by flow cytometry, and the ratio of CD4/CD8 was calculated. RESULTS: After tracheotomy, the levels of β-defensin-2 mRNA and β-defensin-2 in lung tissue from the group B were increased significantly at 24 h, then they were decreased gradually, and decreased most significantly at 168 h (<0.05). The content of β-defensin-2 in peripheral blood of group B decreased gradually, and the content of β-defensin-2 in 168 h was significantly lower than that in 24 h (<0.05), but there was no significant difference between group B and group A (>0.05). The level of CD3 T cells in peripheral blood was significantly lower than that in the group A (<0.05), but their was no significant difference in CD4 and CD8 T cells compared with group A (>0.05). After ICA intervention in group C: lung tissue, alveolar lavage fluid, peripheral blood serum β-defensin-2 content, and peripheral blood CD3 and CD4 T cell levels were gradually increased, significantly higher than those in the group B (<0.05). CD8 T cell level was significantly lower than that in the group A at 24 h (<0.05), the CD4/CD8 ratio was significantly higher at 168 h than those in the group A or B (both <0.01). CONCLUSIONS ICA can improve the early lung immune function in rats with tracheotomy, which might be related to up-regulation of β-defensin-2 in lung tissue and alveolar lavage fluid, concomitant with increases in CD3 and CD4 T cells and CD4/CD8 ratio in peripheral blood while reduction in CD8 cells.
Animals ; Flavonoids ; Male ; Rats ; Rats, Sprague-Dawley ; T-Lymphocyte Subsets ; Tracheotomy ; beta-Defensins

Objective: To analyze the clinical features of respiratory epithelial adenomatoid hamartoma (REAH) in the nasal cavity. Methods: Clinical datas of 23 patients, who were hospitalized in Department of Otorhinolaryngology Head and Neck Surgery, the Affiliated Hospital of Qingdao University, from February 2016 to February 2018 with histopathologically proved REAH in the nasal cavity were reviewed. There were 18 males and 5 females, with the age range from 16 to 71 years old. The most common area, main symptoms, surgical methods and follow-up results were analyzed. Results: The most common area was olfactory cleft (21 cases), and the mainly symptom was nasal congestion (18 cases). All the patients received transnasal endoscopic surgery and had no recurrence during following-up from three months to one year. Conclusions The most common area of REAH in the nasal cavity is the olfactory cleft. Histopathological result is needed to make a definite diagnosis. Complete surgical resection is the main treatment.

Aging increases the risk of various diseases. The main goal of aging research is to find therapies that attenuate aging and alleviate aging-related diseases. In this study, we screened a natural product library for geroprotective compounds using Werner syndrome (WS) human mesenchymal stem cells (hMSCs), a premature aging model that we recently established. Ten candidate compounds were identified and quercetin was investigated in detail due to its leading effects. Mechanistic studies revealed that quercetin alleviated senescence via the enhancement of cell proliferation and restoration of heterochromatin architecture in WS hMSCs. RNA-sequencing analysis revealed the transcriptional commonalities and differences in the geroprotective effects by quercetin and Vitamin C. Besides WS hMSCs, quercetin also attenuated cellular senescence in Hutchinson-Gilford progeria syndrome (HGPS) and physiological-aging hMSCs. Taken together, our study identifies quercetin as a geroprotective agent against accelerated and natural aging in hMSCs, providing a potential therapeutic intervention for treating age-associated disorders.

One hundred ten patients with Parkinson′s disease(PD)and 110 age-matched healthy controls were enrolled in the study.The non-motor symptoms(NMS)were assessed with Non-motor symptoms of Parkinson′s disease questionnaire(PDNMSQ30).The PD patients were classified as gait abnormality posture type(PIGD, n=73)and tremor type(TD, n=37).The non-motor symptoms were compared between two groups.Results showed that the NMS in PD patients was higher than that in healthy controls[(35.2 ±3.0)vs.(6.0 ±2.0)symptoms, t=6.43, P=0.01].The NMS score of PIGD group was higher than that of TD group[(28.9 ±1.1)vs.(6.2 ±0.7)symptoms, t=2.73, P<0.01]; Mini-mental state examination(MMSE)and Montreal cognitive assessment scale(MoCA)scores of TD group were significantly higher than those of PIGD group(t=11.52,P<0.01; t=8.79,P<0.01).The results indicate that for PD patients with different motor types,their NMS are different,and PD patients with PIGD have more axial involvement,and more NMS.

Objective To explore and analyze the social support and family burden in patients with chronic hepatitis B (CHB).Methods From January to December 2016, a total of 272 patients with CHB and 272 primary caregivers in a Class Ⅲ Grade A hospital in Nantong were selected by convenience sampling method, and investigated by social support rating scale (SSRS) and family burden scale of disease (FBS). The results were statistically analyzed by SPSS 17.0 software, and the correlation between social support and family burden was analyzed by Pearson correlation analysis.Results The score of social support was (44.99±7.21) and family burden was (16.59±11.03). Pearson correlation analyzsis showed a negative correlation between social support and family burden (r=-0.176,P=0.004). The differences of social support were statistically significant among CHB patients with different marital status, monthly income and medical payment method (t/F=5.499, 11.490, 23.140;P<0.05). The differences of family burden were statistically significant among CHB patients' caregivers with different gender, occupation, education level, relation with patients and time caregiving (t/F= 2.412, 2.180, 7.990, 2.714, 6.372;P<0.05).Conclusions Further interventions should be implemented to improve social support and reduce disease burden for patients with CHB.

Objective To explore the efficacy of group intervention on the medication compliance, the degree of family care, negative emotion in patients with chronic hepatitis B. Methods From July to August 2016, a total of 80 patients with chronic hepatitis B who volunteered to take part in this research were selected and divided into two groups by random table method, with 40 cases in each group. The experimental group received group intervention, while the control group were given routine intervention. The medication compliance, the degree of family care and the negative emotion of the two groups were compared before and after intervention. Results After 6 months' intervention, the medication compliance of the experimental group was (0.67±0.85), and that of the control group was (1.20±1.34). The difference was statistically significant (t=2.097, P< 0.05). Patients with good family function in the experimental group increased from 65.00% to 87.50%. The scores of SAS and SDS were (42.92±9.04) and (45.91±8.89), which were both lower than those of the control group, with statistical significance (t=2.231, 2.226;P< 0.05). Conclusions Group intervention could help increase medication compliance and family care degree, at the same time decrease negative emotion in patients with chronic hepatitis B.

Objective To evaluate the efficacy and safety of Qingpeng ointment for the treatment of localized eczema in children.Methods A multicenter,randomized,open-labeled,tacrolimus 0.03％ ointment-controlled clinical trial was conducted.A total of 442 children with localized eczema were enrolled into this study,and divided into 2 groups to topically apply Qingpeng ointment (Qingpeng group) and tacrolimus 0.03％ ointment (tacrolimus group) respectively twice a day for 2 weeks.The children were followed up before the treatment and 1,2 weeks after the treatment,and the efficacy and safety were evaluated at the same time.Results Clinical data from 426 children were included in the efficacy analysis.At 1,2 weeks after the treatment,there were no significant differences in the response rate between the Qingpeng group and tacrolimus group (1 week after the treatment:78.92％ vs.81.77％,x2 =0.545,P =0.460;2 weeks after the treatment:98.65％ vs.97.54％,x2 =0.721,P =0.396).However,the pruritus scores at 1,2 weeks after the treatment were both significantly lower in the Qingpeng group than in the tacrolimus group (1 week:F =14.001,P =0.000;2 weeks:F =11.252,P =0.001).At 1 week after the treatment,the incidence rate of adverse reactions was significantly lower in the Qingpeng group than in the tacrolimus group (P ＜ 0.05).Mild itching,burning sensation and other local irritant sensations were the most common adverse reactions.Conclusion Qingpeng ointment is safe and effective for the treatment of localized eczema in children with good tolerability.

Objective To investigate the current situation of maternal breastfeeding after the childbirth in Shanghai and analyze its influencing factors, so as to improve breastfeeding.Methods In this study, longitudinal follow-up and questionnaire designed based on the hospital were carried out on pregnant women at the outpatient department in eight baby-friendly hospitals of Shanghai from October 2015 to January 2016. The questionnaires included demographic characteristics and their prospective feeding method after the childbirth. Pregnant women were followed up until the delivery was finished. At the time of hospitalization discharge,they were given the 2nd questionnaire containing the basic situation after the delivery and the actual breastfeeding behavior situation.Results The rates of pregnant women with the intention of breastfeeding and exclusive breastfeeding during the third trimester were 82.5% and 82.3% respectively. After the delivery,56.4% (399/707) of mothers were breastfeeding. Binary logistic regression analysis showed that,for primipara group, breastfeeding behavior after the delivery was related to five factors:the breastfeeding intention in third trimester, age,household register,the time of onset of lactation and whether the baby was in the care unit;for multiparas group,breastfeeding behavior was related to two factors:the time of onset of lactation and whether the baby was in the care unit.Conclusions Health care workers should attach importance to the promotion of breastfeeding during pregnancy for primipara group and pay attention to the health education of the mothers with registeredpermanent residence of Shanghai. During the hospitalization,health care workers should strictly implement the "early sucking,early contact" as the baby-friendly hospital required,strengthen the breastfeeding knowledge and skill training for mothers whose babies are in the maternal care unit,ensure the normal lactation of mothers without their babies so as to improve the early breast-feeding rate after the delivery.

Objective To understand the medication compliance,family care and their correlation in patients with chronic hepatitis B. Methods From January to Decemeber 2016,two hundred and seventy-six chronic hepatitis B patients from a class III grade A infectious disease hospital in Nantong were selected as the research object by convenient sampling method. They were investigated by the medication compliance questionnaire and the family care index questionnaire. Results Chronic hepatitis B patients with good medication compliance accounted for 48.91%,the family care score was(7.82±2.38)points. Pearson correlation analysis showed negative correlation between the medication compliance of patients and family care(r=-0.173, P < 0.05). There were significance differences in medication compliance among different age,gender, occupation,cultural level,monthly income,medical payment(χ2=27.45,8.42,13.36,24.76,10.65,18.38;P < 0.05). There were significant differences in family care scores among patients of different age,gender, occupation,marital status,monthly income and method of medical payment(t=7.41,2.52,4.96,6.88,5.65, 11.26;P<0.05). Conclusions The medication compliance and family care of chronic hepatitis B patients still needs to be further improved. It is necessary to strengthen the multi family-oriented nursing intervention,and improve medication compliance and family care so as to delay progression and improve quality of life of patients.