Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective:To design and develop a WeChat mini-program for risk assessment and health management of people at high-risk of stroke.Methods:Relying on the network platforms and smartphones, a WeChat mini-program based health management network platform was designed and developed based on the analysis of the needs of high-risk stroke populations and medical staff for the management of high-risk stroke populations, with health data collection, stroke risk assessment, health education and knowledge base matching and intervention programs and data privacy and security as the key technologies.Results:The mini-program included three ports, such as the patient end, medical staff end and backend management end. It had the main functions of stroke risk assessment, intelligent promotion of health management follow-up plans, recording of health indicators and medication reminders, interactive consultation, and health knowledge promotion.Conclusions:The development of risk assessment and health management WeChat mini-program for high-risk stroke populations and the use of a simple and effective intelligent assessment to realize rapid classification of stroke risk in population lay a foundation for the WeChat mini-program function module to carry out individualized, accurate and efficient brain-heart health management.

OBJECTIVE To investigate the anti-tumor effects of the components of Ganoderma lucidum(Gc) based on the two-dimensional(2D) and three-dimensional(3D) culture of colorectal cancer HCT116 cells. METHODS The chemical compositional of the three components was identified by UPLC-Q-TOF-MS. An in vitro 3D culture model of HCT116 cells was established by using Matrigel as the matrix material, and the effects of Gc1, Gc2, Gc3, and 5-fluorouracil(5-FU) on the proliferation of HCT116 cells in 2D and 3D culture models were evaluated, and the effects of Gc3 on cell-cycle, apoptosis, drug resistance, lipid metabolism, and 5-FU's anti-tumor activity were evaluated. Cell viability was detected by CCK-8 assay. mRNA expression level of the cells was analyzed by Real-time PCR. Proteins expression level of the cells was analyzed by Western blotting. HPLC was used to detect the content of 5-FU in cells. RESULTS A total of 76, 69, and 17 compounds were identified from Gc1, Gc2, and Gc3, respectively. Compared with 2D culture, the proliferation rate of HCT116 cells was decreased in the 3D culture model, and the expression of cell cycle-promoters CDK2, CDK4, CDK6, and fatty acid synthesizer FASN, SREBP1 were significantly down-regulated. On the contrary, the expression of cell cycle-suppressor p21, p27, and lipid droplet breakdown proteins ATGL and drug resistance gene ITGB1, CDH1, ABCB1, and ABCC1 mRNA were significantly up-regulated. Gc1, Gc2, Gc3 and 5-FU inhibited the proliferation of both 2D and 3D cultured HCT116 cells in a dose dependent manner after incubation for 48 h, and the inhibitory effect of Gc3 was significantly stronger than Gc1 and Gc2. Gc3 could not only reduce the expression of CDK2, CDK4, Bcl-xl, ATGL, and LC3B proteins, but also increase the expression of p21, p27, Bax, Cleaved caspase-3, and Cleaved PARP1 proteins, and overexpression of LC3B or ATGL attenuated Gc3-induced cytotoxicity in 3D cultured HCT116 cells. In addition, Gc3 significantly inhibited the expression of ITGB1, CDH1, ABCB1, and ABCC1 mRNA, and increased the intracellular 5-FU content, and enhanced the anti-tumor activity. CONCLUSION Gc3 significantly inhibit the proliferation of 3D-cultured HCT116 cells by inhibiting cell autophagy and lipid droplet breakdown, and enhance the anti-cancer activity of 5-FU by inhibiting the expression of ITGB1, CDH1, ABCB1, and ABCC1 mRNA.