Objective:To explore the differences in clinical and injured vertebra radiological parameters between patients with non-traumatic osteoporotic vertebral compression fracture (OVCF) and those with traumatic OVCF after percutaneous vertebroplasty (PVP).Methods:A retrospective study was conducted to analyze the 369 OVCF patients (with 458 vertebrae injured) who had been treated by PVP at Department of Orthopaedics, Beijing Luhe Hospital between October 2015 and March 2017. There were 292 females and 77 males with a mean age of 73 (60, 79) years. Based on the absence or presence of a trauma history, the patients were stratified into a non-traumatic group (127 patients with 160 vertebrae injured) and a traumatic group (242 patients with 298 vertebrae injured). Clinical parameters [age, gender, body mass index, symptomatic duration, and number of injured vertebrae, visual analogue scale (VAS), Oswestry disability index (ODI), duration of follow-up, and rate of new OVCFs] and injured vertebra radiological parameters (position of injured vertebra, fracture type, compression severity, fracture range, cortical defect, intravertebral cleft, spinal canal compromise, basivertebral foramen, morphology of bone cement, range of bone cement, cement leakage, cement volume, rate of vertebral height restoration, recollapse of cemented vertebrae) were recorded perioperatively. All the clinical and radiological parameters were compared between the 2 groups.Results:Compared with the traumatic group, the non-traumatic group had an older age [75 (71, 83) years versus 71 (65, 76) years], more females (85.0% versus 76.0%), a longer symptomatic duration [10.0 (7.0, 15.0) d versus 6.5 (2.0, 12.0) d], a lower preoperative VAS pain score [7 (6, 8) points versus 7 (7, 8) points], a lower VAS pain score at postoperative day 1 [2 (2, 3) points versus 2 (2, 3) points], a lower preoperative ODI [66% (63%, 72%) versus 70% (65%, 73 %)], a lower ODI at postoperative day 1 [32% (30%, 34%) versus 32% (31%, 34%)], a higher rate of new OVCFs during follow-up (34.6% versus 12.8%), a lower rate of thoracolumbar lesions (51.9% versus 70.1%), more deformed fractures (mostly amphicoelous type), a lower rate of cortical defects in the anterior wall (20.0% versus 31.5%), a higher rate of trabecular pattern of cement (83.1% versus 71.8%), a higher rate of type-B cement leakage (50.6% versus 31.9%), a lower rate of type-C cement leakage (5.6% versus 12.8%), a lower rate of recollapse of cemented vertebrae (43.8% versus 55.4%). All the comparisons above were statistically significant ( P<0.05). There were no significant differences between the 2 groups in the other clinical or radiological parameters ( P>0.05). Conclusions:There are statistically significant differences in a significant number of clinical and injured vertebra radiological parameters between patients with non-traumatic OVCF and those with traumatic OVCF after PVP. It is noteworthy that non-traumatic OVCFs are one specific subgroup of OVCFs.

Objective To explore the effects of airflow stimuli on human immunoglobulin concentrations in a warm environment,and to discuss and analyze the correlations between immunoglobulin concentrations and subjective questionnaires results.Methods Dynamic airflow of different modes was created by a modified wind blowing device in the climate chamber.Then the changes of S-IgA and S-IgE concentrations of 12 subjects exposed to different airflow environments were measured,during which the TSV,TCV,draught perception,satisfaction with the airflow,and airflow discomfort symptom polling of the subjects were investigated.Results The S-IgA concentration increased by 56.3%after blowing in the warm environment,but the subsequent high air speed condition resulted in a decrease of S-IgA concentration by 24.8%.Both S-IgA and S-IgE had the highest concentrations when the TSV was neutral,and the S-IgA concentration was 176%higher under comfortable conditions than that under uncomfortable conditions.The S-IgA concentration showed an inverted"U"-shaped relationship with the average wind speed,and the highest S-IgA concentration was observed when the mean air speed was 1.0 m/s.Conclusion Dynamic airflow has a significant effect on S-IgA concentration but little effect on S-IgE concentration.Comfortable airflow in a warm environment could benefit the S-IgA concentration elevation thus the respiratory immunity.However,a strong draught perception caused by high wind speeds and an unsatisfactory airflow environment can lead to lower S-IgA concentrations.

Objective:To compare the effects of unilateral thoracic paravertebal block with lidocaine on hemodynamic and the level of consciousness during double lumen endotracheal intubation.Methods:From June to october 2021,a total of 40 patients American Society of Anesthesiologists(ASA)physical status Ⅰ-Ⅱ,aged 19-65 years,scheduled for elective thoracic sugeries in Peking University Interna-tional Hospital block with under general anesthesia requiring orotracheal intubation were recruited and di-vided into two groups:The double-lumen endobronchial intubation(group C)and double-lumen endo-bronchial intubation after thoracic paravertebal block with lidocaine(group P).After an intravenous an-esthetic induction,the orotracheal double-lumen intubation was performed using a Macintosh direct laryn-goscopy,respectively.Invasive blood pressure(BP)and heart rate(HR)were recorded before and after anesthetic induction,immediately after intubation and 5 min after intubation with 1-minute interval and the intubation time was also noted.Rate-pressure product(RPP)were calculated.Results:After anes-thetic induction,BP and RPP in the two groups decreased significantly compared with their preinduction values.As comparison with their postinduction values,the orotracheal intubation in the two groups caused significant increases in BP,HR and RPP.Diastolic blood pressure(DBP)and mean arterial pressure(MAP)increased significantly and lasted for 1-minute in group C compared with the baseline values.Systolic blood pressure(SBP)was not significant change and DBP increased significantly immediately af-ter intubation in group P.HR of both groups after intubation were significantly higher than their baseline values and lasted for 4 min in group C,HR increased significantly immediately after intubation in group P.SBP,DBP,MAP,HR and RPP after intubation in group P were significantly lower than those of group C during the observation period.The value of BIS was similar between the two groups.Compared with group C,the incidence of SBP greater than 30％and RPP greater than 22 000 was significantly lower in group P in the observation period,and no patient in group P developed RPP greater than 22 000.At the end of the incidence of SBP less than 30％of the basal value and HR less than 30％of the baseline,no severe bradycardia occurred in both groups.Conclusion:During double-lumen endobronchial intubation,unilateral thoracic paravertebal block with lidocaine can provide less hemodynamic response and level of conscionsness.

Objective:To investigate the anatomical significance of four groups of small vascular arches, which distributed in the anterior, posterior, medial and lateral of axillary in radical bromhidrosis surgeries.Methods:One hundred and fifty patients treated for bromhidrosis at Qianjiang Central Hospital of Chongqing from February 2015 to August 2018 were included in the study , including 80 males and 70 females. Sixty patients were aged 10-16 years old, and 90 were aged 17-49 years old. There were 21 mild cases, 43 moderate cases and 86 severe cases. The area of axillary hair was marked before surgery, and then the surgical incision was selected between the anterior and posterior folds of the middle part of the axilla. The anterior, posterior, medial, and lateral axillary small vascular arches were identified, and the minimum distance between the vascular arch and the margin of axillary hair area was measured. The tissues with a diameter of about 1 cm were removed at the lateral, medial (close to the vascular arch), 1 cm medial, 2 cm medial to the vascular arch and the surface of the axillary fascia for pathological examination. The superficial fascia including the axillary hair follicles in the area enclosed by the four vascular arcs were then completely cut off with tissue scissors. The specimen was examined microscopically after HE staining to determine whether it was apocrine sweat gland. If there were apocrine sweat glands, it was counted as 1, and if there were no apocrine sweat glands, it was counted as 0. The proportion of specimens diagnosed as apocrine sweat glands was counted. The patients were followed up to evaluate the incision healing and the residual bromhidrosis.Results:All the 150 cases were successfully completed, and it was found that the position of the 4 groups small vascular arch was relatively consistent. The range defined by the axillary small vessel arch was all larger than the axillary hair area, and the minimum distance between the axillary small vessel arch and the margin of axillary hair in 90.00%(270/300) patients was more than 1.5 cm. The apocrine sweat glands were found in 0% (0/1 200) of the samples taken from at 1cm lateral to vascular arch, 1.33%(16/1 200) at medial vascular arch (close to vascular arch), 100.00% (1 200/1 200)at 1 cm and 2 cm media to vascular arch. And the apocrine sweat glands on the surface of the axillary fascia were 0% (0/1 200). All patients were cured postoperatively, without residual glands or recurrence. All patients had good postoperative wound healing without hematoma and skin necrosis. After 6 months to 3 years follow-up, all the patients were cured without residual or recurrence.Conclusions:The four groups of small vascular arches, anterior, posterior, internal and external of axilla, haveanatomical significance in standardizing radical bromhidrosis surgery.

Objective:To investigate the anatomical significance of four groups of small vascular arches, which distributed in the anterior, posterior, medial and lateral of axillary in radical bromhidrosis surgeries.Methods:One hundred and fifty patients treated for bromhidrosis at Qianjiang Central Hospital of Chongqing from February 2015 to August 2018 were included in the study , including 80 males and 70 females. Sixty patients were aged 10-16 years old, and 90 were aged 17-49 years old. There were 21 mild cases, 43 moderate cases and 86 severe cases. The area of axillary hair was marked before surgery, and then the surgical incision was selected between the anterior and posterior folds of the middle part of the axilla. The anterior, posterior, medial, and lateral axillary small vascular arches were identified, and the minimum distance between the vascular arch and the margin of axillary hair area was measured. The tissues with a diameter of about 1 cm were removed at the lateral, medial (close to the vascular arch), 1 cm medial, 2 cm medial to the vascular arch and the surface of the axillary fascia for pathological examination. The superficial fascia including the axillary hair follicles in the area enclosed by the four vascular arcs were then completely cut off with tissue scissors. The specimen was examined microscopically after HE staining to determine whether it was apocrine sweat gland. If there were apocrine sweat glands, it was counted as 1, and if there were no apocrine sweat glands, it was counted as 0. The proportion of specimens diagnosed as apocrine sweat glands was counted. The patients were followed up to evaluate the incision healing and the residual bromhidrosis.Results:All the 150 cases were successfully completed, and it was found that the position of the 4 groups small vascular arch was relatively consistent. The range defined by the axillary small vessel arch was all larger than the axillary hair area, and the minimum distance between the axillary small vessel arch and the margin of axillary hair in 90.00%(270/300) patients was more than 1.5 cm. The apocrine sweat glands were found in 0% (0/1 200) of the samples taken from at 1cm lateral to vascular arch, 1.33%(16/1 200) at medial vascular arch (close to vascular arch), 100.00% (1 200/1 200)at 1 cm and 2 cm media to vascular arch. And the apocrine sweat glands on the surface of the axillary fascia were 0% (0/1 200). All patients were cured postoperatively, without residual glands or recurrence. All patients had good postoperative wound healing without hematoma and skin necrosis. After 6 months to 3 years follow-up, all the patients were cured without residual or recurrence.Conclusions:The four groups of small vascular arches, anterior, posterior, internal and external of axilla, haveanatomical significance in standardizing radical bromhidrosis surgery.

Tripartite motif (TRIM) family proteins are important effectors of innate immunity against viral infections. Here we identified TRIM35 as a regulator of TRAF3 activation. Deficiency in or inhibition of TRIM35 suppressed the production of type I interferon (IFN) in response to viral infection. Trim35-deficient mice were more susceptible to influenza A virus (IAV) infection than were wild-type mice. TRIM35 promoted the RIG-I-mediated signaling by catalyzing Lys63-linked polyubiquitination of TRAF3 and the subsequent formation of a signaling complex with VISA and TBK1. IAV PB2 polymerase countered the innate antiviral immune response by impeding the Lys63-linked polyubiquitination and activation of TRAF3. TRIM35 mediated Lys48-linked polyubiquitination and proteasomal degradation of IAV PB2, thereby antagonizing its suppression of TRAF3 activation. Our in vitro and in vivo findings thus reveal novel roles of TRIM35, through catalyzing Lys63- or Lys48-linked polyubiquitination, in RIG-I antiviral immunity and mechanism of defense against IAV infection.
A549 Cells ; Animals ; Apoptosis Regulatory Proteins/immunology* ; DEAD Box Protein 58/immunology* ; Dogs ; HEK293 Cells ; Humans ; Influenza A Virus, H1N1 Subtype/immunology* ; Madin Darby Canine Kidney Cells ; Mice ; Mice, Knockout ; Orthomyxoviridae Infections/pathology* ; Proteolysis ; RAW 264.7 Cells ; Signal Transduction/immunology* ; THP-1 Cells ; TNF Receptor-Associated Factor 3/immunology* ; Ubiquitination/immunology* ; Viral Proteins/immunology*

OBJECTIVE: To explore the clinical and genetic characteristics of a patient with 17-hydroxylase/17,20-lyase deficiency. METHODS: The patient was infertile without contraception. Laboratory examination showed her chromosomal karyotype to be 46, XX. DNA sequencing was performed to detect variants of CYP17A1 gene in the patient and her family members. RESULTS: Sanger sequencing revealed that the patient has carried homozygous variant c.1486C>T in the exon 8 of the CYP17A1 gene, which resulted in substitution of arginine by cysteine (p.Arg496Cys). Her family members were all heterozygotes for the same variant. CONCLUSION Homozygous variant of the CYP17A1 gene c.1486C>T probably underlay the 17-hydroxylase deficiency in this patient. Above finding has enabled accurate genetic counseling and prenatal diagnosis for her family.

Objective:Endoplasmic reticulum stress(ERS)was used as the research emphasis to further investigate the mechanisms of apoptosis of FLT3-ITD-mutated leukemia cells and decreased expression of FLT3-ITD mutated protein induced by all-trans retinoic acid(ATRA).Methods:FLT3-ITD-mutated leukemia cell lines(MV4-11 and MOLM13)were treated with ATRA. Flow cytometry was conducted to assess cell apoptosis. Real-time fluorescent quantitative PCR(RT-qPCR)and Western blot were used to detect the expression of ERS-related and autophagy-related genes and protein, respectively.Results:A low-dose ATRA further increased FLT3-ITD cells and ERS levels. ATRA acted on the ERS-related PERK/eif2ɑ signaling pathway and continued to increase the ERS of FLT3-ITD cells, resulting in an upregulation of apoptotic gene CHOP expression. After the treatment with ATRA, FLT3-ITD protein in FLT3-ITD cells was decreased. Of the two main ERS-related protein degradation pathways, ER-associated degradation(ERAD)and ER-activated autophagy(ERAA), the expression of ERAD-related protein ATF6 in FLT3-ITD cells was not significantly changed on ATRA, whereas the expression of ERAA-related proteins Atg7 and Atg5 were significantly increased.Conclusions:ATRA further raises the ERS level of FLT3-ITD cells continuously by activating the ERS-related PERK/eif2ɑ signal pathway and induces FLT3-ITD protein autophagy degradation through ERAA pathway, which induces apoptosis of FLT3-ITD-mutated leukemia cells. These results provide preliminary evidence on the use of ATRA in the treatment of refractory leukemia with FLT3-ITD.

OBJECTIVE: To analyze the clinical features of chronic myeloid leukemia (CML) with T315 I mutation (CML-T315I) and compare the effectiveness of different treatments. METHODS: We retrospectively analyzed the clinical data and outcomes of 19 patients with CML-T315I receiving different treatments. The T315 I mutations in these patients were detected by examination of BCR-ABL kinase domain (KD) mutation by RTQ-PCR and Sanger sequencing. The relapse following the treatments, defined as hematological, cytogenetic and molecular biological recurrences, were analyzed in these patients. RESULTS: Of the 19 patients with CML-T315I, 14 (73.7%) were in CML-CP stage at the initial diagnosis, and 13 (81.2%) were high-risk patients based on the Sokal scores. All the 19 patients were treated with TKI after the initial diagnosis, and during the treatment, 15 (78.9%) patients were found to have additional chromosomal aberrations, and 10 (52.6%) had multiple mutations; 13 (68.4%) of the patients experienced disease progression (accelerated phase/blast crisis) before the detection of T315I mutation, with a median time of 40 months (5-120 months) from the initial diagnosis to the mutation detection. After detection of the mutation, 12 patients were treated with ponatinib and 7 were managed with the conventional chemotherapy regimen, and their overall survival rates at 3 years were 83.3% and 14.2%, respectively ( < 0.001). CONCLUSIONS CML patients resistant to TKI are more likely to have T315I mutations, whose detection rate is significantly higher in the progressive phase than in the chronic phase. These patients often have additional chromosomal aberrations and multiple gene mutations with poor prognoses and a high recurrence rate even after hematopoietic stem cell transplantation. Long-term maintenance therapy with ponatinib may improve the prognosis and prolong the survival time of the patients.
Drug Resistance, Neoplasm ; Fusion Proteins, bcr-abl ; Humans ; Imidazoles ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; Mutation ; Pyridazines ; Retrospective Studies

Objective: To compare the efficacy of different decompressions treatment combined with posterior cervical fusion and internal fixation treatment on Chiari I malformation associated with syringomyelia and atlantoaxial instability. Methods: From January 2016 to October 2017, 36 cases of Chiari I malformation associated with syringomyelia and atlantoaxial instability in Linfen People's Hospital were selected in the research.The patients were divided into two groups according to random number table method, with 18 cases in each group.The observation group was given decompression of posterior fossa of small bone window + cerebellar tonsillectomy + posterior cervical fusion and internal fixation treatment.The control group was given traditional decompression of posterior fossa + posterior cervical fusion and internal fixation treatment.The efficacy, complications occurred within 1 year after operation, JOA score and spinal cord cavity size at different time points before and after operation of the two groups were compared. Results: The total effective rate of the observation group [94.44%(17/18)] was slightly higher than that of the control group [88.89%(16/18)] (χ²=1.957, P>0.05). The total effective rate of the observation group at 1 year after operation was 88.89%(16/18), which was significantly higher than that of the control group [72.22%(13/18)] (χ²=3.498, P<0.05). The incidence rate of complications of the observation group at 1 year after operation was 11.11%(2/18), which was significantly lower than that of the control group [38.89%(7/18)](χ²=11.685, P<0.05). The JOA scores at discharge[(15.97±1.25)point] and 1 year after operation[(15.53±1.19)point] of the observation group were significantly higher than those of the control group[(14.21±1.18)point, (14.06±1.15)point](t=3.815, 3.706, all P<0.05). The spinal cord cavity size at discharge [(2.13±0.64)mm] and 1 year after operation[(2.28±0.69)mm] of the observation group were significantly higher than those of the control group [(3.41±0.76)mm, (3.45±0.78)mm](t=5.743, 5.511, all P<0.05). Conclusion The long-term efficacy of decompression of posterior fossa of small bone window + cerebellar tonsillectomy + posterior cervical fusion and internal fixation treatment on Chiari I malformation associated with syringomyelia and atlantoaxial instability is remarkable, the recovery of spinal cord function and syringomyelia is well and the postoperative complications incidence is relatively low, which is worthy of popularization and application.