Objective: To explore the mechanism of Yinao Jieyu Compound Formula in the treatment of post-stroke stressrats from the perspective of intestinal flora changes. Methods: A total of 48 male clean SD rats were randomly divided into4 groups: sham-operation group, post-stroke stress group, western medicine group and traditional Chinese medicinegroup, with 12 rats in each group. In the sham-operation group, only the free blood vessels were not inserted into thethread embolus. The rats in post-stroke stress group, the western medicine group and the Chinese medicine group weretreated as follows: after 1 week of middle cerebral artery occlusion, the rat model of PSD was prepared by using chronicrestraint stress and single cage feeding. The 7 th day from the beginning of the construction of the cerebral stroke model, the gavage treatment began to be done. The western medicine group was given Fluoxetine Hydrochloride Capsules2.33 mg/ (kg.d), and the Chinese medicine group was given 9.92 g/ (kg·D) . The rest of the rats were gavage with 10 mL/kgdistilled water, 1 time/day. The behavioral changes on the 14 th day after post-stroke were observed and the microbialcommunity structure and composition was analyzed by Illumina-MiSeq high throughput sequencing technique. Results:Compared with the sham operation group, the sucrose water preference, open field score and swimming struggle time ofthe post-stroke stress group were significantly decreased (P ＜ 0.01, P ＜ 0.01, P ＜ 0.01), and the proportions ofAllobaculum, Sutterella, Dorea and rc4-4 in the feces decreased significantly (P ＜ 0.01, P ＜ 0.01, P ＜ 0.01, P ＜ 0.05) .The proportion of Oscillospira increased significantly (P ＜ 0.01, P ＜ 0.05, P ＜ 0.01) . Compared with the post-strokestress group, the horizontal scores and swimming struggling time of the rats in the Chinese medicine group weresignificantly increased (P ＜ 0.05, P ＜ 0.05), and the proportions of the 6 species of Lactobacillus and Bacteroides weresignificantly increased in the feces. The difference between Lactobacillus and Bacteroides was the most obvious with27.91％ vs 5.96％ and 24.21％ vs 9.62％ (P ＜ 0.01, P ＜ 0.01) . The proportion of Oscillospira was significantly decreased (P ＜ 0.05) . Compared with the western medicine group, there was no significant difference in the behavioral scores of therats in the traditional Chinese medicine group (P＞ 0.05), and the proportion of Bacteroides and rc4-4 in the feces wassignificantly increased (P ＜ 0.01, P ＜ 0.05) . Conclusion: Yinao Jieyu Compound Formula can alleviate similar depressivesymptoms in post-stroke stress rats by increasing the content of Lactobacillus and Bacteroides in the intestine of rats.

As a new type of functional material, magnetic thermosensitive polymeric microspheres offer high potential application in various fields, particularly in bioengineering and biomedical fields. In this review, the development of synthesis and application of magnetic thermosensitive polymeric microspheres was summarized, and the research trends were also discussed.
Biocompatible Materials ; chemistry ; Magnetics ; Microspheres ; Particle Size ; Polymers ; chemistry ; Temperature