Objective:In this study, the collected mosquito samples were subjected to viral isolation to identify the species and branch characteristics of arboviruses in five regions of Shanxi Province.Methods:Eight arboviruses in mosquito samples collected from July to September 2020 were detected by real-time fluorescent quantitative PCR, and virus isolation was carried out through cell culture. Virus isolates were identified and analyzed by molecular biology and bioinformatics method.Results:We detected 1 batch of positive samples of Japanese encephalitis virus, 2 batches of positive samples of Culex flavivirus and 8 batches of positive samples of Nam Dinh virus among 121 batches of mosquito samples. Seven virus isolates were isolated, numbered: SX-YJ-Cxp-4、SX-YJ-Ars-2、SX-YJ-Cxp-1、SX-LY-Cxp-10、SX-GP-Ars-5、SX-GP-Cxp-2、SX-GP-Cxp-4, all of which were identified as Nam Dinh virus, and the whole genome sequencing was performed on one of them, and the result showed that Shanxi Nam Dinh virus isolate and Yunnan Nam Dinh virus isolate belonged to the same evolutionary branch.Conclusions:Nam Dinh virus was isolated and identified on the specimen from Shanxi province for the first time.

The formation of most proteins consists of two steps: the synthesis of precursor proteins and the synthesis of functional proteins. In these processes, propeptides play important roles in assisting protein folding or inhibiting its activity. As an important polypeptide chain coded by a gene sequence in lipase gene, propeptide usually functions as an intramolecular chaperone, assisting enzyme molecule folding. Meanwhile, some specific sites on propeptide such as glycosylated sites, have important effect on the activity, stability in extreme environment, methanol resistance and the substrate specificity of the lipase. Studying the mechanism of propeptide-mediated protein folding, as well as the influence of propeptide on lipases, will allow to regulate lipase by alternating the propeptide folding behavior and in turn pave new ways for protein engineering research.
Lipase/metabolism* ; Molecular Chaperones/metabolism* ; Protein Folding ; Protein Precursors ; Substrate Specificity

On October 3rd, 2017, one male patient, aged 27 years, was admitted to our hospital 6 hours after hydrothermal scald of torso, buttocks, and limbs. The total area of burn was about 60% total body surface area, and the depth was from deep partial-thickness burn to full-thickness burn. Immediately after admission, the patient was given symptomatic support treatments, such as anti-shock, fluid replacement, and anti-infection, etc. After being treated by debridement and xenogenic (porcine) skin grafting for 2 times, the wounds were healed well. On the 12th day of admission, linezolid was used to prevent infection according to the results of microbial culture and drug sensitivity test, since when the level of his blood lactate continued to increase. After 8 days, linezolid was discontinued and vitamin B1 was given orally for 1 week, and the level of lactic acid gradually decreased to normal in result. This case was used mainly to analyze whether linezolid could directly cause hyperlacticemia and its important mechanism, aiming at reminding clinicians of being alert to the risk of hyperlacticemia when using linezolid. If hyperlacticemia occurs, linezolid should be discontinued immediately and vitamin B1 should be taken orally to correct the high lactic acid value, and the treatment plan should be adjusted if necessary.

Objective? To sum up the experience on application automated dispensing cabinet (ADC) in ward drug administration to improve the management level and work efficiency. Methods? From January to December 2017, a total of 22 wards used the cabinets in Peking University International Hospital. The drug administration had three categories including usual, base and narcotics drugs. We compared the time of getting drug before and after applying ADC by counting the mean of single time of getting drug in temporary medical order through the year. Results? Among 22 wards, the time of getting drug in temporary medical decreased from (17.55±3.92) min to (3.73±0.83) min after applying ADC with a statistical difference (t=16.160, P＜ 0.01). Besides, there was no adverse event caused by getting drugs mistakenly after applying ADC. Conclusions? ADC uses the intelligent management in ward drug administration which can improve working efficiency of nurses, realize refined management of drugs, improve medication safety of patients and reduce the waste of drugs.

Objective? To explore the effects of the establishment and application of "Five in one"stepwise psychological rehabilitation model for cancer patients. Methods? In February 2016, the Department of Chest Oncology in West China Hospital Sichuan University established "Five in One" stepwise psychological rehabilitation model: a team consisting of oncology doctors, nurses, medical social workers, sunshine nurses/psychologist and psychiatrist who provided holistic psychological management including psychological assessment, psychological education, and psychological intervention. By convenience sampling, a total of 1 402 cancer patients admitted before the establishment of the model in the hospital from August 2015 to January 2016 were selected and put into the control group. Routine nursing intervention was given to the group. Totally 1 823 cancer patients admitted from February to July in 2016 after the establishment of the model in the hospital were selected and assigned into the observation group. The "Five in One" model was adopted for the group. The two groups were compared in terms of the conditions of adverse emotions and suicide risks. Results? The incidence of adverse emotions was 29.24%, and the incidence of suicide risk was 3.92% in the control group. The incidence of adverse emotions was 10.92% and 1.76% for the suicide risk in the observation group. There were statistical differences in the incidence of adverse emotions and suicide risks between the two groups (P＜0.01). Conclusions? The establishment of "Five in One" model for cancer patients can reduce cancer patients' adverse emotions and risks of suicide, and provides new ideas and methods for the management of cancer patients' psychological distress.

Objective: To investigate the effect of ubiquitous mitochondrial creatine kinase 1(CKMT1) on the sensitivity of human nasopharyngeal carcinoma cell line CNE-1 to DDP. Methods: CNE-1 cells were transiently transfected with CKMT1 overexpression (CKMT1) or empty vector (EV). The growth curve and DDP IC50 were developed by MTT assay, plate clone formation assay was performed by gradient concentration of DDP treatment, cell cycle and apoptosis were detected by flow cytometry, levels of apoptosis related protein Bax/Bcl-2/C-PARP and the transcription factor p-STAT3-Tyr705 were detected by Western Blot. Results: The transfection efficiencies of CKMT1 and EV were more than 90% with a higher proliferation rate in the CKMT1-transfected cells. However, the CKMT1-transfected cells had a DDP IC50 of 2.76 μmol/L, which was significantly lower than that of 4.60 μmol/L in the EV-transfected cells (P<0.01). With the treatment of certain concentration of DDP, the CKMT1-transfected cells had a lower clone formation rate, the cell cycle arrested more obviously in G2/M phase, and the apoptosis rate was higher (P<0.01), with higher levels of Bax/C-PARP (P<0.05 or P<0.01), but lower levels of Bcl-2 (P<0.01) and p-STAT3-Tyr705 (P<0.01), compare with the EV-transfected cells. Conclusions CKMT1 may inhibit the activation of STAT3, increasing the sensitivity of CNE-1 to chemotherapeutic drug DDP.

OBJECTIVETo observe the effects of electroacupuncture (EA) on pain behavior in rats with bone cancer pain and morphine tolerance, and to explore partial action mechanism.

METHODSForty-two SD healthy female rats were randomly divided into a sham operation group (7 rats), a bone cancer pain group (8 rats), a morphine tolerance group (9 rats), an EA group (9 rats) and a sham EA group (9 rats). The rats in the sham operation group were treated with injection of phosphate buffer saline at medullary cavity of left-side tibia, and the rats in the remaining groups were injected with MRMT-1 breast cancer cells. After operation, no treatment was given to rats in the sham operation group and bone cancer pain group. 11 days after operation, rats in the morphine tole-rance group, EA group and sham EA group were treated with intraperitoneal injection of morphine hydrochloride, once every 12 hours, for 11 days to establish the model of bone cancer pain and morphine tolerance. One day after the establishment of this bone cancer pain model, the rats in the morphine tolerance group were injected with morphine, once every 12 hours (9:00 a.m. and 9:00 p.m.) for 7 days; the rats in the EA group and sham EA group were injected with morphine at 9:00 a.m., and treated with EA (2 Hz/100 Hz) and sham EA (only injected into the subcutaneous tissue) at bilateral "Zusanli" (ST 36) and "Kunlun" (BL 60), 30 min per treatment, once a day for 7 days. One day before cancer cell injection, 6 days, 8 days, 10 days after operation, after 30 min on 1 days, 5 days, 9 days, 11 days of morphine injection, and after 30 min on 1 days, 3 days, 5 days, 7 days of EA treatment, the paw withdrawal threshold (PWT) was measured in each group. On 11 day of morphine injection, HE staining was applied to observe the morphology and structure change of tibia in the sham operation group, bone cancer pain group and morphine tolerance group, random 2 rats in each group. On 7 days of EA treatment, fluorescent immunohistochemical method was applied to observe the expression of μ-opioid receptor positive cells in nucleus ceruleus in each group, random 4 rats in each one.

RESULTSAfter 10 days of the cancer cells injection, the PWT of 28 rats of bone cancer pain model (8 rats in the bone cancer pain group, 8 rats in the morphine tolerance group, 6 rats in the EA group and 6 rats in the sham EA group) was significantly lower than that of 7 rats in the sham operation group (<0.01). After one day of morphine injection, the PWT of the morphine tolerance group, EA group and sham EA group was higher than that of the bone cancer pain group (all<0.01); on 11 d of morphine injection, the PWT of the morphine tolerance group, EA group and sham EA group was not significantly different from that of the bone cancer pain group (all>0.05). On 11 d of morphine injection, the tumor induced by cancer cells was observed in upper 1/3 tibia in the bone cancer pain group and morphine tolerance group, and the marrow cavity was filled with MRMT-1 cancer cells; no abnormal change was observed in the sham operation group. On 1 d, 3 d, 5 d and 7 d of EA treatment, the PWT of the cancer pain group, morphine tolerance group and sham EA group was lower than that of the EA group (all<0.01). On 7 d of EA treatment, the positive expression of MOR in nucleus ceruleus in the cancer pain group, morphine tolerance group, EA group and sham EA group was lower than that in the sham operation group (<0.01,<0.05), and that in the cancer pain group, morphine tolerance group and sham EA group was lower than that in the EA group (all<0.01).

CONCLUSIONSEA can improve mechanical pain threshold in rats with bone cancer pain-morphine tolerance, and improve the abnormal pain, which is likely to be involved with improvement of the MOR positive cells expression in nucleus ceruleus by EA.

Objective To explore the effect of the pattern of nurse-patient double-check and signature in security management of intravenous administration and to understand the degree of satisfaction of patients with safe medication management.Methods The experience on security management of intravenous administration in inpatients from Oncology Department in the West China Hospital of Sichuan University were summarized from December 2015 to January 2017. Inpatients with intravenous administration between December 2015 and June 2016 acted as control group. Inpatients with intravenous administration between July 2016 and January 2017 acted as improved group along with the management method of the pattern of nurse-patient double-check and signature. The situation on security management of intravenous administration and the degree of satisfaction of patients with safe medication was compared between two groups.Results After implementing the pattern of nurse-patient double-check and signature along with intravenous administration, there was no medication administering errors (MAE). The degree of satisfaction of patients with safe medication raised from 94.20 % to 100.00% with a significant difference (P< 0.01).Conclusions The pattern of nurse-patient double-check and signature realizes the goal of patients' involvement in medical safety, ensures the infusion safety. It is worth to be used widely in clinical application.

Objective To explore the effects of the motivational interview on medication adherence and control level of asthma in adult patients with asthma. Methods A total of 100 asthma patients who were hospitalized in the Respiration Department of Putuo People's Hospital in 2016 were selected in the study using convenience sampling. According to the random number table, the participants were divided into the observation group (n=50) and the control group (n=50). Two cases from the observation group withdrew from the study due to personal reasons, leaving 48 patients in the observation group. Three cases from the control group withdrew from the study (i.e. two for disease changes and one for personal reasons), leaving 47 cases in the control group. The control group received conventional health education while the observation group received education based on motivational interview. The asthma medication compliance scale (Chinese version) (MARS-A) and asthma control test (ACT) questionnaire were used to evaluate the medication compliance and asthma control level before the intervention and 1, 2 and 3 months after the intervention. Results None of the patients had good medication compliance before the intervention. However, patients in the observation group had significantly better adherence at 1, 2, and 3 months after the intervention compared with the control group (P<0.05). There were no significant differences in asthma control level between two groups before intervention (P>0.05). But after the intervention, the repeated variance analysis showed that there were significant differences in both intervention effect and time effect for asthma control score, and the interaction effects existed between intervention and time (P< 0.05). Conclusions The health education based on motivational interview can effectively improve the medication adherence and the control level of asthma in adult patients with asthma.

Objective To explore the effects of network platform health education in the patients with cervical cancer after the operation of cervical cancer patients. Methods We selected 54 cases of cervical cancer surgery patients from June 2014 to February 2015 in our hospital for treatment according to the voluntary principle. The patients were divided into observation group and control group by random numbers, and the patients of observation group accepted continue nursing health education network platform while the patients of control group accepted routine care. The complication rate, re-hospitalization rate and treatment satisfaction of the two groups were compared. Results The complications rate and re-hospitalization rates of observe group were 0. 00%, and these of the control group were 14. 81% and 18. 52% (P<0. 05). Observation group′s satisfaction was 96. 30%, while that of the control group was only 59. 26% (P<0. 05). Conclusions Health education network platform for nursing care to a certain extent can reduce cervical cancer patient the incidence of postoperative complications,re-hospitalization rates, and increase the patients health knowledge, the effects of treatment and treatment satisfaction.