Objective : To investigate the effects of milk derived pentapeptide prolin⁃glycine⁃proline⁃isoleucine⁃pro⁃ line ( PGPIP) on chronic alcoholic liver injury in mice and its related molecular mechanism . Methods : Forty C57BL/6 mice were randomly divided into control group , model group , glutathione(GSH) group , PGPIPN group .The mice model of chronic alcoholic fatty liver was established by 10 d ad libitum oral feeding with the Lieber⁃DeCarli(LD) ethanol liquid diet plus one binge . Drug intervention was given at the same time . Based on the reported RNA sequencing data in gene expression omnibus (GEO) database , the differential expression of liver endoplasmic reticulum stress⁃related genes was analyzed by cluster heat map . Liver hematoxylin⁃eosin (HE) staining was used to analyze the pathological effects of each treatment group on alcoholic liver injury in mice . Oiled Red O staining was used to analyze the effects of each treatment group on the accumulation of liver lipid droplets in mice . Transtransduction protein expression was detected by Western blot . Results : The pathological examination of PGPIP group was similar to that of Control group , and the liver injury of mice was significantly reduced . The accumulation of lipid droplets in the liver of mice in the model group was manifested as mixed lipid droplets of different sizes , and PGPIP treatment significantly reduced the accumulation of liver lipid droplets induced by alcohol . PGPIP had significant effects on the PERK⁃eIF2α⁃ATF4 pathway and the expression of transcriptional activator 6 ( ATF6 ), Cleaved Caspase 3 protein . Conclusion Pentapeptide PGPIP can alleviate chronic alcoholic fatty liver and liver injury in mice . The mechanism may be attributed to reducing the accumulation of lipid droplets in hepatocytes , en⁃ doplasmic reticulum stress , and hepatocyte apoptosis .

Objective : To investing ate the milk-derived hexapeptide PGPIPN and its truncated pentapeptide PGPIP to alleviate chronic alcoholic liver injury in mice and its associated molecular mechanisms． Methods : Sixty Kun- ming mice were randomly divided into control group，model group，GSH group，PGPIPN group，and truncated pen- tapeptide PGPIP group．The model of chronic alcoholic liver injury in mice was established by gavage with gradient alcohol． Drug intervention was given at the same time for 12 weeks． Liver HE staining was used to analyze the pathological effects of each treatment group on alcoholic liver injury in mice．Primary mouse hepatocytes and human normal hepatocyte line L-02 were isolated and cultured in vitro．The appropriate PGPIPN induction concentrations of both cells were determined by WST-1 method． L-02 cells were induced at different times． The expression of FoxO3a and phosphorylated FoxO3a protein were detected by Western blot to determine the appropriate induction time．The subcellular localization of FoxO3a in L-02 cells was detected by cellular immunofluorescence．The mR- NA changes of FoxO3a and MnSOD genes in primary hepatocytes and L-02 cells of mice in different treatment groups were detected by qRT-PCR. Results : The pathological examination of PGPIPN group and PGPIP group was similar to that of GSH group，and the liver injury of mice was significantly reduced．Medium and high concentra- tions of PGPIPN were respectively selected to induce mouse primary hepatocytes and L-02 cells．At 16 hours，the expression of FoxO3a protein in L-02 cells increased significantly．FoxO3a protein was mainly expressed in the nu- cleus．In addition，mRNA levels in both types of cells increased significantly after induction with the corresponding dose of PGPIPN． Conclusion PGPIPN and truncated pentapeptide PGPIP can reduce chronic alcoholic liver injury in mice．The mechanism may be to reduce alcohol-induced oxidative stress through FoxO3a-MnSOD signaling path- way.

Objective: To investigate the clinical efficacy and safety of Shure granule combined with estradiol valerate in the prevention of intrauterine adhesions in induced abortion.Methods: Selected 240 cases of artificial abortion were divided into observation group and control group according to the order of admission, 120 cases each.Patients in the control group were treated with estradiol valerate.The observation group was treated with Shure granules on the basis of the control group;The treatment time was 3 weeks.The time of vaginal bleeding and the amount of bleeding, menstruation, retidal and adverse drug reactions of the two groups were observed;The levels of serum fibronectin (FN), human laminin (LN) and vascular endothelial growth factor (VEGF) were compared between the two groups before and after the operation.The adhesion of the 12 cases in the two cases was compared.Results: The time of vaginal bleeding and the amount of bleeding in the observation group were significantly better than those in the control group (P＜0.05), and the normal rate of menstrual volume was higher than that of the control group (P＜0.05).The serum levels of FN, LN and VEGF in the observation group were significantly lower than those in the control group 2 weeks after the operation (P＜0.05).After 12 months, the incidence of intrauterine adhesions in the observation group was significantly lower than that in the control group (P＜0.05).There was no significant difference in the incidence of adverse drug reactions between the two groups (P＞0.05).Conclusion: The efficacy of Shure granule combined with estradiol valerate in preventing intrauterine adhesions in induced abortion is superior to estradiol valerate alone.