Objective To utilize the Mendelian randomized(MR)method to investigate the causal relationship between helicobacter pylori(Hp)infection and the development of diabetes mellitus,providing reference value for understanding the pathogenesis of extragastrointestinal diseases following Hp infection.Methods We employed two single nucleotide polymorphisms(SNPs)that have been previously reported as genetic variants associated with Hp seroprevalence,along with publicly available genome-wide association studies(GWAS)data.We used the inverse variance-weighted(IVW)technique,MR-Egger regression,and weighted median technique for the analysis.Results The IVW results indicated no causal relationship between genetic susceptibility to Hp infection and an increased risk of diabetes(OR=1.018,95％CI:0.984-1.054,P=0.304).Moreover,none of the Hp-related antibodies,including VacA(OR=1.007,95％CI:0.986-1.028,P=0.537),CagA(OR=0.988,95％CI:0.967-1.010,P=0.298),and IgG(OR=0.973,95％CI:0.939-1.008,P=0.129),showed a causal association with the risk of diabetes.Heterogeneity and horizontal pleiotropy tests were conducted,and the results indicated no heterogeneity or horizontal pleiotropy,confirming the reliability of the MR analysis.Conclusion The findings of this study suggest that there is no causal relationship between Hp infection and an increased risk of diabetes.