Objective:To investigate the relationship between iodine nutrition level and thyroid disease.Methods:Totally 299 patients with thyroid disease who were treated at Shandong Provincial Institute for Endemic Disease Prevention and Control from 2016 to 2018 were selected as case group which was further divided into Graves' disease group (GD group, 137 patients), chronic lymphocytic thyroiditis group (HT group, 90 patients) and thyroid nodule group (72 patients). At the same time, 75 healthy people with no history of thyroid disease, normal thyroid color ultrasound and thyroid function were selected as control group. Morning urine was collected and urinary iodine was detected by arsenic cerium catalytic spectrophotometry. Fasting venous blood was extracted, and serum levels of thyroid stimulating hormone (TSH), free triiodothyronine (FT 3), free thyroxine (FT 4), thyroid globulin antibody (TgAb) and thyroid peroxidase antibody (TPOAb) were detected by electrochemiluminescence method. Results:The difference of median urinary iodine in the 4 groups was statistically significant ( H = 42.530, P < 0.05). The medians urinary iodine in GD and HT groups (326.79, 341.91 μg/L) were higher than those of thyroid nodule group and control group (235.01, 187.32 μg/L, P < 0.05). The levels of TSH, FT 3 and FT 4 in GD group were compared with those of control group, and the differences were statistically significant ( P < 0.05). The positive rates of TgAb and TPOAb in HT group were significantly higher than those in GD, thyroid nodule and control groups, and the positive rates of TgAb and TPOAb in GD group were higher than those in thyroid nodule and control groups ( P < 0.05). Conclusions:GD and HT patients have excessive iodine nutrition, and high iodine intake may lead to the occurrence of these thyroid diseases (GD and HT). Thyroid function test combined with laboratory urinary iodine test can be used to diagnose thyroid diseases simply and quickly.

Objective: To understand the status of iodine nutrition of children aged 8-10 years and pregnant women in Shizuishan City. Methods: In 2017, five sampling districts were divided into east, west, south, north and middle districts in three counties (Dawukou District, Huinong District and Pingluo County) of Shizuishan City, one township (town, street) was selected in each district, one primary school was selected in each township (town, street), 40 children aged 8-10 years were selected in each primary school, and 20 pregnant women were selected in each township (town, street). In Dawukou District and Huinong District of Shizuishan City, 50 g of edible salt and 10 ml of urine samples were collected from the homes of children and pregnant women; in Pingluo County 50 g of edible salt samples were collected from the homes of children and pregnant women. Iodine in edible iodized salt was measured by redox titration and urinary iodine content was measured by arsenic-cerium catalytic spectrophotometer. Results: A total of 900 edible salt samples were collected, the median iodine content in salt was 24.50 mg/kg, the coverage rate of iodized salt was 97.67% (879/900), the qualified rate of iodized salt was 86.92% (764/879), and the edible rate of qualified iodized salt was 84.89% (764/900). A total of 400 urine samples of children were tested, the median urinary iodine was 213 μg/L. There was statistically significant difference in urinary iodine among children between Dawukou District and Huinong District (the median urinary iodine was 246 and 194 μg/L, respectively, Z=-4.827, P < 0.05), but no statistically significant differences in urinary iodine among children in different gender and age groups (P > 0.05). A total of 200 urine samples of pregnant women were tested, and the median urinary iodine was 173 μg/L. There were no significant differences in urinary iodine content between pregnant women in different counties(districts), and between pregnant women at different pregnancy stages (P > 0.05). Conclusion The iodine nutrition status of the children aged 8-10 years and pregnant women in Shizuishan City is generally at the appropriate level.

Objective To explore the drug sensibility of Brucella from bovine and sheep in Xinjiang.Methods Using paper diffusion method,19 drugs of 8 kinds of antibiotics including aminoglycosides,macrolides,sulfonamides,tetracyclines,β-lactams,fluoroquinolones,chloramphenicols and rifamycins,were tested.Drug sensitivity test was conducted on 57 Brucella strains isolated from bovine and sheep in Xinjiang from 2010 to 2016.Results The 57 Brucella strains were highly sensitive to doxycycline,tetracycline,streptomycin,tobramycin,gentamicin,amikacin,amoxicillin,ofloxacin,fleroxacin,ciprofloxacin and chloramphenicol,with the sensitivity rates were all higher than 90％;and they were highly resistance to azithromycin,clarithromycin and bactrim,with the drug resistance rates were all higher than 80％.Conclusion Brucella from bovine and sheep in Xinjiang is sensitive to tetracyclines,aminoglycosides,β-1actams,fluoroquinolones and chloramphenicols.

Objective To evaluate the effect of oxycodone on microglial activation in brain tissues of rats. Methods Primarily cultured microglial cells of Sprague?Dawley rats were seeded in 24?well plates (1 ml∕well)at a density of 1×105cells∕ml and divided into 5 groups(n=40 each)using a random num?ber table: control group(group C), lipopolysaccharide(LPS)group(group L)and low, medium and high concentrations of oxycodone groups(O25, O50, O100groups). The cells were cultured in serum?free medium for 24 h in group C. LPS was added at the final concentration of 1 μg∕ml in L, O25, O50and O100 groups, and in addition oxycodone was added at the final concentration of 25, 50 and 100 ng∕ml at 24 h of incubation with LPS in O25, O50and O100groups, respectively. Cells were collected at 1 h of incubation or culture for determination of the expression of tumor necrosis factor?α(TNF?α), interleukin?1β(IL?1β), IL?10 and transforming growth factor?1β(TGF?1β)mRNA(by real?time polymerase chain reaction)and expression of TGF?1β and phosphorylated Smad2(p?Smad2)(using Western blot). The concentrations of TNF?α, IL?1β and IL?10 in the supernatant were detected by enzyme?linked immunosorbent assay. Results Compared with group C, the expression of TGF?β1, IL?1β and TNF?α mRNA, TGF?β1 and p?Smad2 was significantly up?regulated, and the concentrations of IL?1β, IL?10 and TNF?α in the supernatant were increased in group L(P<0.01). Compared with group L, the expression of TGF?β1, IL?1β and TNF?α mRNA, TGF?β1 and p?Smad2 was significantly down?regulated in O50and O100groups, the expression of IL?10 mRNA was significantly up?regulated, and the concentrations of IL?1β and TNF?α in the supernatant were decreased in group O100(P<0.05), and no significant change was found in the parameters mentioned above in group O25(P>0.05). Conclusion Oxycodone can inhibit microglial activation in brain tissues of rats.

Objective To evaluate the effect of dexmedimidine on intestinal injury in rats with endotoxemia.Methods Twenty-four healthy adult male Sprague-Dawley rats,aged 2-3 months,weighing 200-250 g,were divided into 4 groups (n =6 each) using a random number table method:control group (group C),endotoxemia group (group E),dexmedimidine group (group D) and dexmedimidine plus α7 subunit-containing nicotinic acetylcholine receptor antagonist group (D +α-BGT group).The endotoxemia model was established by injecting lipopolysaccharide (LPS) 10 mg/kg via the femoral vein.Dexmedetomidine 40 μg/kg was injected and 15 min later LPS was intravenously injected in group D.Dexmedetomidine 40 μg/kg was intraperitoneally injected after intraperitoneal injection of α-bungarotoxin 1 μg/kg,and 15 min later LPS was intravenously injected in group D+o-BGT.Blood samples were collected from the abdominal aorta at 6 h after LPS injection for determination of the plasma interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) concentrations (by enzyme-linked immunosorbent assay).Rats were sacrificed after blood sampling,and intestinal tissues were obtained for examination of the pathological changes and for determination of the myeloperoxidase (MPO) activity (by chemical colorimetry) and expression of NF-κB p65 in nucleoprotein (by Western blot).Results Compared with group C,the plasma IL-6 and TNF-o concentrations and MPO activity in intestinal tissues were significantly increased,and the expression of NF-κB p65 in nucleoprotein was up-regulated in the other 3 groups (P＜0.05).Compared with group E,the plasma IL-6 and TNF-α concentrations and MPO activity in intestinal tissues were significantly decreased,the expression of NF-κB p65 in nucleoprotein was down-regulated (p＜0.05),and the pathological changes of intestinal tissues were significantly attenuated in group D.Compared with group D,the plasma IL-6 and TNF-α concentrations and MPO activity in intestinal tissues were significantly increased,the expression of NF-κB p65 in nucleoprotein was up-regulated (P＜0.05),and the pathological changes of intestinal tissues were accentuated in group D+α-BGT.Conclusion Dexmedetomidine can reduce the intestinal injury in rats with endotoxemia,and the mechanism may be related to activating cholinergic anti-inflammatory pathway and further inhibiting inflammatory responses.

Several studies have demonstrated the role of 4-1BBL in T cell activation. Furthermore, enhanced 4-1BB/4-1BBL interaction has been shown to amplify T-cell-mediated antitumor immunity in several mouse models. However, when applied in humans, it was difficult to generate sufficient T cells ex vivo and whole cell vaccines to transfer back into patients. To overcome this difficulty, we have focused on producing the human 4-1BBL extracellular domain/anti-CD20 Fab' fusion protein. In this report, PCR and overlap PCR were used to construct the human 4-1BBL extracellular domain/anti-CD20 Fab' expression vector. DNA sequence was analyzed by the Terminus of Dideoxy Nucleotide. The product was purified by affinity chromatography and analyzed by SDS-PAGE and HPLC; its antigen binding activity was examined by rosetting assay. The data of DNA sequence showed that the human 4-1BBL extracellular domain/anti-CD20 Fab' fusion protein was corrected. The fusion protein was recovered in high yield (up to 200 microg/mL) after E-taq purification. The fusion protein was capable of simultaneous binding to stimulated Jurkat cells and Raji cells as shown by cellular rosetting. In conclusion, the human 4-1BBL extracellular domain/anti-CD20 Fab' fusion protein was induced to express in E. coli 16C9. The results of some biological activity experiments indicated that the fusion protein could bind to stimulated Jurkat cells and Raji cells. Furthermore, 4-1BBL-negative tumors can be converted into 4-1BBL-positive tumors by the fusion protein without the need for 4-1BBL gene transfer to the malignant cells.
4-1BB Ligand ; biosynthesis ; genetics ; Antibodies, Bispecific ; immunology ; Antigens, CD20 ; immunology ; Humans ; Immunoglobulin Fab Fragments ; biosynthesis ; genetics ; Immunotherapy ; methods ; Lymphoma, Non-Hodgkin ; therapy ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; immunology