Objective To observe the expression of adhesion molecule CD226 on the small intestinal group 3 innate lymphoid cells (ILC3) in mice. Methods The bioinformatics was used to analyze the expression of CD226 on murine ILCs. Small intestinal mucosal lamina propria lymphocytes (LPL) were isolated from wild-type C57BL/6J mice, and the expression of CD226 on ILC1 and ILC3 was detected by flow cytometry. A mouse model of dextran sulfate sodium (DSS)-induced colitis was constructed to observe the changes in the expression of CD226 on ILC3. Results Both ILC1 and ILC3 in the mice small intestine expressed CD226 molecules; the proportion of ILC3 was reduced, while the expression level of CD226 on ILC3 was increased in the colitis model. Conclusion CD226 is expressed on the small intestines of mice, and although the proportion of ILC3 decreases in the DSS-induced colitis, the expression of CD226 on ILC3 increases.
Animals ; Mice ; Colitis/chemically induced* ; Immunity, Innate ; Intestine, Small ; Lymphocytes ; Mice, Inbred C57BL

Objective To explore the CT manifestation and the diagnostic value of leiomyomatous tmnor of gastrointestinal tract in 8 patients.Methods 8 patients with gastrointestinnal tract leiomyoma tumor were scaned with GE HISPEED CT/I.proceeding to scan with contrast agent when detected and ascertained the nidus.Results There were 4 eases gastric smooth muscle tumors in 8 patients,and 1 case was leiomyosarcoma of stomach,1 case was duodenal muscle tumors,1 case as jejuna muscle tumors and another is leiomyosarcoma of jejunum.Conclusion The location,contour and characteristic of imaging is important standard of leiomyomatous tumor of gastrointestinal tract.

Objective To explore diagnosis value of spiral CT in ultra-acute large area cerebral infarction. Methods 60 patients with clinical history and CT manifestation of ultra-acute large area cerebral infarction underwent conventional CT scans without contrast enhancement within the first 6 hours after onset of neurological symptoms and signs were retrospectively analyzed. Results In CT plain scan,the density of brain parenchymal reduced in 49 cases,regional cerebral tissue swelled in 38 cases,one side of the middle cerebral artery showed high- density sign in 18 cases,normal sign showed in 11 cases;21 cases were diagnosed in hyperacute cerebral infarction by conventional CT(diagnosis rate 35%). Conclusion Spiral CT had high diagnosis value on ultra-acute large area cerebral infarction.

Objective To investigates the diagnostic value of CT of the gallbladder and accuracy.Methods Clinical data of 26 patients with pathologically confirmed gallhladder were retrospectively analyzed.Results 26 patients were diagnosed with CT.CT manifestations of primary gallbladder carcinoma:papillary intraluminal mass type,limited or diffuse gallbladder wall thickening,large cystic mass in full or swallowed type.Combined with the direct signs of pathology gallbladder cancer was divided into three types:infiltrative,nodular,tumor type;Enhance a performance of CT scan of gallbladder:artery of high density,such as density of the portal vein,followed by arterial and portal vein of both high-density,higher than normal latency of the gallbladder wall and liver,regression not significant,especially gallbladder junction local thickening of the gallbladder wall continued enhanced features.Conclusion The performance of primary gallbladder was complex,and CT examination was a key means of establishing diagnosis.

Objective: To investigate the effects of hyperbaric oxygen(HB0_2) on black pigmented bacteroides groups(BPB), obligately and facultatively anaerobes of periodontitis. Methods: 60 periodontitis cases were divided into 2 groups. The HB0_2 group(gingival scaling was done at the first visit)was exposed in 0.25 MPa pure oxygen 90 minutes each day, and treated 10 days continuously. The control group received gingival scaling at the first visit. The clinical indices of each group were measured. The microorganisms in periodontal pocket were incubated and analyzed. The number of subgingival anaerobes as well as the number of BPB,obligately and facultatively anaerobes were measured and counted by routine anaerobic culture. Results: Highly significant differences in Gingival Indices (GI), Probing Depth (PD), Attachment Loss (AL), and also significant differences in Plaque Index (PLI),and teeth mobility were seen between the HB0_2 group and the control group. There were significant difference of PLI, GI,PD,AL, teeth mobility in HB0_2 group pre-therapy and post-therapy. There were significant difference of subgingival anaerobes amounts, obligately and facultatively anaerobes sorts between pre-therapy and post-therapy. The positive rate of BPB of group HB0_2 post-therapy were reduced more than that of pre-therapy(P<0.01). Conclusion: HB0_2 has beneficial therapeutic effects on chronic and aggressive periodontitis. The mechanism of the HB0_2 therapy might inhibit the growth of subgingival obligately and facultatively anaerobes and BPB of human periodontitis, especially the obligately anaerobes and BPB in periodontal pockets.

OBJECTIVETo study the effects and the therapeutic mechanism of hyperbaric oxygen (HBO) on prostaglandin E(2) (PGE(2)) in alveolar bone and gingiva of experimental periodontitis in animal.

METHODSExperimental periodontitis was produced by silk thread sutures combined with high content sugar diet. For HBO therapy, they were exposed to a pressure of 0.25 MPa (2.5ATA), breathing pure oxygen one session a day for 60 min. The treatment course was 2 weeks. The value of PGE(2) in gingiva and alveolar bone was analyzed by enzyme immunoassay (EIA).

RESULTSThe value of PGE(2) in gingiva of control group was 3.21 ng/g, and that of PGE(2) in alveolar bone was 3.22 ng/g. The contents of PGE(2) in gingiva (13.96 ng/g) and alveolar bone (13.32 ng/g) of periodontitis group increased markedly than control group (P < 0.01). The contents of PGE(2) in gingiva (5.21 ng/g) of HBO group were 62.7% which was lower than that of periodontitis group, and the value of PGE(2) in alveolar bone (4.05 ng/g) were 69.6% lower than that of periodontitis group. The difference of PGE(2) in gingiva or alveolar bone was significant for the HBO group and periodontitis group (P < 0.01).

CONCLUSIONSThe contents of PGE(2) in alveolar bone and gingiva increased markedly when experimental periodontitis has formed. The value of PGE(2) in alveolar bone and gingiva reduce markedly after HBO exposure, and the decreased rate of PGE(2) in alveolar bone is more evident than that of PGE(2) in gingiva after HBO therapy.

Alveolar Process ; metabolism ; Animals ; Dinoprostone ; metabolism ; Disease Models, Animal ; Female ; Gingiva ; metabolism ; Guinea Pigs ; Hyperbaric Oxygenation ; Male ; Periodontitis ; metabolism

To study the effect of hyperbaric oxygen (HBO) on cellular proliferative nuclear antigen (PCNA) in spinal cord after its injury (SCI), we observed the pathological changes and the expression change in PCNA in spinal cord after the injured rats were treated with HBO. The results showed that there were hemorrhage, edema, vacuolation of nerve cells in spinal cord, disorder and fragmentation in nerve fibers after injury. PCNA was strongly positively expressed in neurons. The results in 0.1MPa HBO group were similar to that of non-treatment group, while in 0.25MPa HBO group, edema was abolished, the cellular vacuolation was lessened, the morphology of nerve cells recovered, the structural configuration appeared normal, and the degree of PCNA expression was decreased compared with the injury group. These results suggested that PCNA could serve as a cellular proliferative index; HBO could prevent or alleviate the pathological changes of SCI, and could effectively promote the repair of the injury of the spinal cord.

Objective: To study the effects of hyperbaric oxyge(HBO) in the treatment of periodontitis.Methods: 60 Guinea pigs were divided into 3 groups, 20 animals each group: ①Experimental periodontitis was induced by silk suture and feeding with the food containing 100 g/L of sugar and then exposed to 0.25 MPa of HBO for 60 min once a day when periodontitis established; ② the other 20 animals were simillary treated to group ① but not exposed to HBO; ③ another 20 animals were used as controls. HBO treatment was conducted for 2 weeks. Samples of periodontal tissues were obtained 0,4,8 and 10 weeks after treatment and prepared for histopathological study and electron microscopic observation.Results: In the treated group there were fewer macrophages in gingiva and periodontal ligment, more blood vessles, less absorption of alveolar bone, more osteoblasts and more osteogenesis in the bone. There were fewer metochodria and rough endo reticulum in plasmacytes, macrophages and osteoclasts; and more in fibroblasts, endothelial cells of blood vessles and osteoblasts. Conclusion: HBO may improve blood supply, inhibit macrophage and bone absorption.

Objective:To study the effects of periodontal scaling on microorganism in periodontal pocket and gingival crevicular fluid in patients with periodontitis.Methods:60 cases of periodontitis were selected and divided into 2 groups randomly with 30 in each group.The patients in scaling group were treated by periodontal scaling, those in control group by gargle with chlohexidine.Before and after treatment the microorganism in pocket bottom of each patient was measured by Congo red negative dyeing.Gingival index(GI), probing depth(PD) and gingival crevicular fluid(GCF) were also measured, the relationship between periodontal scaling and clinical indices was analysed.Results:In scaling group the percentage of coccoid cells in pocket bottom increased more after scaling,that of bacillus,spirochetes decreased, PD,GCF value were all decreased (P0.05).Conclusion:Periodontal scaling can decrease the percentage of pathogenesis bacteria in periodontal pocket bottom and decrease PD,GI and GCF values.

Objective To investigate the relationship of apoptosis of microglia and neuron in adult rats following fast decompression. Methods A model of decompression sickness in rat was reproduced by exposure to 1MPa 5.5min followed by rapid decompression (50s). Brain tissues were collected at 0h, 6h, 24h, 48h and 72h after decompression. The microglia were examined after histochemical staining with FITC-conjugated Isolectin-B4. Cell apoptosis was detected by in situ end labeling TUNEL methods. Results A few IB4-positive microglia could be seen in the brain tissue collected 6h after decompression, and the number of IB4-positive microglia was greatest at 24h (P