Objective: To explore the clinical value of color doppler ultrasonography combined with CA125 and AFP in the early diagnosis of placental abruption. Methods: From January 2015 to December 2017, 120 patients with placental abruption in the Maternal and Child Health Hospital of Zhoushan were selected as observation group.120 healthy pregnant individuals were selected as control group.They all received the color Doppler ultrasound and detection of serum CA125 and AFP levels.The clots checking on the surface of placenta post-delivery was conducted at the same time.The statistical analysis was set up to compare two groups according to those tests from the lab reports. Results: The levels of serum CA125 and AFP were higher in the observation group compared with those in the control group.In details, the CA125[(69.1±8.9)U/mL]and AFP[(279.8±41.3)μg/L] levels in placental abruption grade Ⅲ were significantly higher than those in gradeⅡ[CA125(61.6±9.4)U/mL, AFP(234.9±46.2)μg/L] and gradeⅠ[CA125(52.2±8.9)U/mL, AFP(205.7±43.1)μg/L](all P<0.01). The positive predictive values of placental abruption by the color Doppler ultrasound alone was 46.7%.The combination of the color Doppler ultrasound with serum CA125 was 81.7%.The combination of the color Doppler ultrasound with serum AFP was 78.3%.The combination of those three tests was increased up to 90.8%, which was the best pre-diagnosis compared to the color Doppler ultrasound alone and the other two combinations(χ²=11.67, P<0.01). The detective rate of combination of the color Doppler ultrasound with serum CA125 and AFP was higher than the other two combinations(χ²=12.56, 12.64, all P<0.01). Conclusion The levels of serum CA125 and AFP are positively correlated with placental abruption.The combination tests using the color Doppler ultrasound and both serum CA125 and AFP is a novel and sensitive method as to pre-diagnose high risk placental abruption during pregnancy.

Objective: To establish the method for determination of taurocholic acid in the Traditional Chinese Medicine Shedanchuanbei Oral Liquid and snake bile. Methods: The sample was prepared as mixed solution containing methanol and KH 2PO 4. The mixed solution was injected into Sep Pak C 18 cartridge for the purpose of sample purity. In this processing, the substances which having strong retain action and could harm analytic column were hold in the Sep Pak C 18 cartridge. The eluting solution that the Sep Pak C 18 cartridge had be over loading for taurocholic acid was used as the test solution. The test solution was measured by RP HPLC. The chromatographic conditions were as followed: Supelcosil LC 8 column(150mm?4.6nm,5?m) as analytic column, detect wavelength at 203nm, and MeOH 0.4%KH 2PO 4 mixed solution(56∶44, V/V ) as mobile phase. The inject volume was 50?L. Results: The linear response range of sodium taurocholate was from 0.0253mg?mL -1 to 0.253mg?mL -1 , and the correlation coefficient was 0.9999. The average recovery rate was 101.3%, RSD was 0.40%( n =6). Conclusion: This method was simple, efficient and suitable to the quality control for Shedanchuanbei Oral Liquid and snake bile.