AIM: To design and construct recombinant epitope nucleotides vaccine of glycoprotein B(gB)and glycoprotein D(gD)of herpes simplex virus type 1(HSV-1), and to investigate its immunoprotective effects and tissue expression in animal models.METHODS: The HSV-1 gB and gD epitope genes were selected and tandem assembled to construct the recombinant protein-coding gene X, which was transducted into the prokaryotic expression vector pET28(a). The recombinant protein was synthesized and utilized to generate monoclonal antibodies, which were subsequently used to immunize New Zealand white rabbits. The immunogenicity of the purified protein and the presence of polyclonal antibodies in the serum were tested through separating serum from cardiac blood, and the serum antibody titers were determined. The pcDNA3.1-X was successfully constructed as a eukaryotic expression vector and immunized the female BALB/c mice aged 4 to 6 wk via intramuscular injection. Serum antibodies and immune-related cytokines were quantified using enzyme-linked immunosorbent assay(ELISA). The expression of the X protein in the ocular, trigeminal ganglion, and brain tissues of the mice was assessed.RESULTS: The target polyclonal antibody was identified with a serum antibody titer of 1:3200 in the rabbit serum after immunized by recombinant protein X. Upon immunizing mice with the eukaryotic recombinant plasmid pcDNA3.1-X, the concentration of HSV-1 serum IgM antibodies of the experimental group was 12.13±0.85 ng/L, which was significantly higher than that of the vector control group(0.49±0.44 ng/L; t=21.07, P<0.001). The concentrations of cytokines interleukin IL-2, IL-4, IL-10, and IFN-γ in the experimental group were 11.63±0.60, 22.65±1.47, 85.75±14.12, and 114.90±6.39 ng/L, respectively, all of which were significantly higher than those in the vector control group and the blank control group(all P<0.05). Immunohistochemical staining revealed the presence of target protein X in the eyeball, trigeminal ganglion, and brain tissue.CONCLUSION: The HSV-1 gB and gD tandem epitope nucleotides vaccine pcDNA3.1-X was successfully constructed, which activates a remarkable immune response and is stably expressed in the eyeball, trigeminal ganglion, and brain tissue. This study provides a foundation for further research of an HSV-1 recombinant antigen epitope tandem vaccine.

Objective:To explore the teaching effect of combining diversified teaching modes with individualized teaching methods in musculoskeletal ultrasound training classes compared with traditional teaching modes.Methods:The 109 trainees participated in the muscle and bone ultrasound training classes from June 2020 to June 2022 were included as the research subjects and divided into the observation group ( n=53) and control group ( n=56). The control group received traditional teaching modes, while the observation group received diversified teaching modes in combination with individualized teaching methods. The differences in teaching satisfaction and final exam scores between the two groups were compared after training. Statistical analysis was conducted using the software SPSS 27.0. Categorical data were presented as cases and percentages, while continuous data were presented as mean±standard deviation. The intergroup differences in continuous variables were compared using the t-test, while the intergroup differences in categorical variables were compared using the chi-square test. Results:There were no significant differences in age, sex, education, and professional title between the control group and the observation group ( P>0.05). The comprehensive satisfaction score of ultrasound teaching was (88.08±6.65) in the observation group and (80.71±8.59) in the control group, with a significant difference between the two groups ( χ2=5.02, P<0.01). Before training, the test scores of the control group and the observation group were (62.61±5.39) and (63.87±7.77), respectively, and there was no significant difference between the two groups ( t=0.98, P=0.330). The final scores, theoretical exam, computer operation, and video reading exam scores of the control group were (84.07±1.49), (82.39±1.97), (85.13±2.55), and (86.79±6.06), respectively, while those of the observation group were (90.06±2.17), (88.28±3.19), (88.92±3.23), and (96.23±5.96); there were significant differences between the two groups in these four indicators ( t=16.75, 11.53, 6.79, and 8.20, respectively; P<0.01). The scores were compared for the two groups of trainees classified based on their unit level, professional title, and educational background. Trainees with the same unit level, professional title, and educational background showed no significant differences in test scores between the two groups, though their final scores were significantly different ( P<0.01). Conclusions:The combination of diversified teaching modes and individualized teaching methods can increase the learning enthusiasm of trainees, improve the teaching effect of training classes, and increase the satisfaction of trainees.

Social anxiety disorder is a common anxiety disorder in which a key feature is interpersonal avoidance accompanied by generalized problems with emotion identification and emotion regulation. In response to the gap in these therapeutic priorities in other therapies, emotion-focused therapy has emerged to fill the part of the treatment of emotions with an emphasis on the relational process. The paper reviews the recent studies related with the treatment of social anxiety disorder with emotion-focused therapy. The results suggest that the main points of social anxiety onset in an emotion-focused perspective are traumatic emotional experiences and self-criticism, and that the therapeutic focus of emotion-focused therapy is on dealing with the traumatic emotional scenario behind the anxiety and exposing it to adaptive emotions to be transformed. It also works better in combination with other more mainstream therapies. This suggests that emotion-focused therapy is a promising approach for the clinical treatment of social anxiety disorder and looks forward to the development of a more integrated treatment approach in the clinic.

Objective To investigate clinical value of high sensitive-cardiac troponin T(hs-cTnT) combined with creatine kinase isoenzyme MB(CK-MB) in the diagnosis of children with myocarditis.Methods From Nov.2014 to Nov.2015,a total of 102 cases of myocarditis,suspected with myocardial damage and without myocardial damage(pneumonia and capillary bronchitis),and 50 healthy children were enrolled.Plasma levels of hs-cTnT and CK-MB were detected and compared.Results The levels of plasma hs-cTnT and CK-MB in children with myocarditis were significantly higher than those without myocarditis and healthy subjects(P<0.05).Hs-cTnT and CK-MB levels in children with myocarditis,less than one month old,were significantly higher than those with age of 1 month to 3 years old(P<0.05).Conclusion Combined detection of hs-cTnT and CK-MB could be with high sensitive and specificity in diagnosis of children with myocarditis,accurately assess the disease condition and improve the therapeutic effect and prognosis,which might be worthy of clinical application.

Objective To observe clinical efficacy of osteoporotic thoracolumbar compression fractures treated by manual reduction with percutaneous vertebroplasty. Methods Totally 82 patients with osteoporotic thoracolumbar compression fractures were randomly divided into treatment group (43 cases) and control group (39 cases). The treatment group received the manual reduction combined with percutaneous vertebral plasty, while the control group only received percutaneous vertebral plasty. Lumbar back pain VAS scores, vertebral anterior height and spine after convex Cobb angle before and after operation in the two groups were compared. Results There was statistical significance among VAS pain score, spine after convex Cobb angle and anterior flange height in the two groups (P<0.05); There was no statistical significance in VAS score in the two groups on the 2nd day. (P>0.05), while there was statistical significance in spine after convex Cobb angle and anterior flange height in the two groups (P<0.05);There was statistical significance among VAS pain score, spine after convex Cobb angle and anterior flange height in the two groups in the 3rd month after operation (P<0.05). Conclusion Manual reduction combined with percutaneous vertebral plasty can improve the lower back pain, restore vertebral body height and correct spine after contex Cobb angle of osteoporotic thoracolumbar compression fractures, which is better than pure percutaneous vertebral plasty.

OBJECTIVETo investigate the influence of miR-148a on cell proliferation of human gastric cancer cell lines MKN45.

METHODSExpression level of miR-148a was detected by qRT-PCR in the carcinoma tissues and the tissues adjacent to carcinoma of 60 patients with gastric cancer. Lentivirus packaging was used to establish MKN45 gastric cancer cell line expressing stable miR-148a as transfection group,and the untransfected MKN45 cell line as the control group. Gastric cancer MKN45 cell proliferation was detected by CCK8 method. The target gene of miR-148a was predicted by targetscan. Expression of target gene was examined by Western blotting.

RESULTExpression of miR-148a in gastric cancer tissues of 54 cases(90.0%, 54/60) decreased, and among them, 37 tissue samples(61.7%, 37/60) decreased by 2 times. mir-148a expression in cancer tissues of patients with lymph node metastasis, vascular invasion and TNM stage III(-IIII( was down-regulated more obviously(P<0.05). From the third day after transfection, the growth of MKN45 cells was significantly inhibited(P<0.01). Gene CDC25B might be the target gene of miR-148a according to the results of targetscan. Western blot showed that CDC25B expression in transfection group was lower as compared to control group.

CONCLUSIONMiR-148a expression is down-regulated in gastric cancer tissues and inhibits gastric cancer cell proliferation. CDC25B may be the target gene of miR-148a that plays a role in tumor suppressor.

Cell Line, Tumor ; Cell Proliferation ; Down-Regulation ; Humans ; Lymphatic Metastasis ; MicroRNAs ; genetics ; Neoplasm Staging ; Reverse Transcriptase Polymerase Chain Reaction ; Stomach Neoplasms ; genetics ; Transfection

Objective To investigate the influence of miR-148a on cell proliferation of human gastric cancer cell lines MKN45. Methods Expression level of miR-148a was detected by qRT-PCR in the carcinoma tissues and the tissues adjacent to carcinoma of 60 patients with gastric cancer. Lentivirus packaging was used to establish MKN45 gastric cancer cell line expressing stable miR-148a as transfection group,and the untransfected MKN45 cell line as the control group. Gastric cancer MKN45 cell proliferation was detected by CCK8 method . The target gene of miR-148a was predicted by targetscan. Expression of target gene was examined by Western blotting. Result Expression of miR-148a in gastric cancer tissues of 54 cases (90.0%, 54/60) decreased, and among them, 37 tissue samples (61.7%, 37/60) decreased by 2 times. mir-148a expression in cancer tissues of patients with lymph node metastasis , vascular invasion and TNM stage Ⅲ-Ⅳ was down-regulated more obviously(P<0.05). From the third day after transfection, the growth of MKN45 cells was significantly inhibited (P<0.01). Gene CDC25B might be the target gene of miR-148a according to the results of targetscan. Western blot showed that CDC25B expression in transfection group was lower as compared to control group. Conclusion MiR-148a expression is down-regulated in gastric cancer tissues and inhibits gastric cancer cell proliferation. CDC25B may be the target gene of miR-148a that plays a role in tumor suppressor.

Objective To investigate the influence of miR-148a on cell proliferation of human gastric cancer cell lines MKN45. Methods Expression level of miR-148a was detected by qRT-PCR in the carcinoma tissues and the tissues adjacent to carcinoma of 60 patients with gastric cancer. Lentivirus packaging was used to establish MKN45 gastric cancer cell line expressing stable miR-148a as transfection group,and the untransfected MKN45 cell line as the control group. Gastric cancer MKN45 cell proliferation was detected by CCK8 method . The target gene of miR-148a was predicted by targetscan. Expression of target gene was examined by Western blotting. Result Expression of miR-148a in gastric cancer tissues of 54 cases (90.0%, 54/60) decreased, and among them, 37 tissue samples (61.7%, 37/60) decreased by 2 times. mir-148a expression in cancer tissues of patients with lymph node metastasis , vascular invasion and TNM stage Ⅲ-Ⅳ was down-regulated more obviously(P<0.05). From the third day after transfection, the growth of MKN45 cells was significantly inhibited (P<0.01). Gene CDC25B might be the target gene of miR-148a according to the results of targetscan. Western blot showed that CDC25B expression in transfection group was lower as compared to control group. Conclusion MiR-148a expression is down-regulated in gastric cancer tissues and inhibits gastric cancer cell proliferation. CDC25B may be the target gene of miR-148a that plays a role in tumor suppressor.

Objective To investigate the effects of different marriage modes of parents on mental health of Mosuo middle school students.Methods Basic information questionnaire and symptom checklist-90 (SCL-90)were adopted to compare and to analyze 226 Mosuo students' mental health condition with regard to different marriage modes of parents.Results (1) In the comparison between the students from Monogamous Marriage families and the students from the Walking marriage families,the mean scores of somatization and depression were higher in the former group than in the latter group(0.73 ± 0.59 vs 0.58 ± 0.46,P＜ 0.05 ;0.87 ±0.65 vs 0.70 ±0.52,P＜ 0.05).(2)Among the students fromWalking marriage families,the mean scores of interpersonal sensitivity,depression,anxiety and phobic anxiety were higher in girls than those in boys (1.10 ± 0.76 vs 0.80 ±0.47,P＜0.05;0.83 ±0.54 vs 0.58 ±0.48,P＜0.05;0.99 ±0.71 vs 0.62 ±0.48,P＜0.05;0.99 ±0.77 vs 0.55 ± 0.53,P＜ 0.05).(3) Among the students from Walking-Marriage families,some students had closer relationship with their fathers,and some were closer to maternal uncles,the mean scores of somatization,obsessivecompulsive factor and hostility were higher in the former sub-group than in the latter sub-group (0.69 ± 0.52 vs 0.43 ±0.34,P＜0.05;1.22 ±0.46 vs 1.01 ±0.59,P＜0.05;0.94±0.66 vs0.65 ±0.57,P＜0.05).Conclusion The mental health status of Mosuo students are different regarding the marriage modes of their parents.In the Walking-Marriage families,the mental health of Mosuo students has relationship with their gender and the male whom the students are closer to.

Hedgehog(Hh) pathway plays a key role in a variety of processes, such as embryogenesis, maintenance of tissue homeostasis, tissue repair and carcinogenesis. Recent studies indicate that the aberrant activation of Hh pathway has been linked to multiple types of human cancer. Here, we present an overview of the processing and secretion of Hh pathway and the role of Hh pathway in gastric cancer.