Objective To investigate whether the effects of venlafaxine on major depression disorder is associated with ankyrin G.Methods Breed Synapsin-Cre1 and Ankyrin3-floxed mice(Ank3 cKO mice).Ank3 cKO mice and wild type mice were randomly divided into model and control groups.All mice in model group and control group were orally administrated with venlafaxine(1 g/L)or the solvent(normal saline,NS)with the volume of 200 μL,respectively.Depression-related behaviors were examined by sucrose preference test(SPT)and Y maze test.The level of ankyrin G and PSD95 in the cortex of four groups were detected by Western blot.The level of ankyrin G and MAP2 in the in hippocampus of four groups were detected by immunohistochemistry method.Results Compared with wt-saline group,the cKO mice in saline showed a significantly decreased preference of sucrose(P＜0.001)and low spontaneous alteration(P＜0.05).Compared with cKO control ones,the venlafaxine model cKO mice showed remarkably increased preference of sucrose(P＜0.001)and more spontaneous alteration(P＜0.05).The level of ankyrin G and PSD-95 in the cortex of venlafaxine cKO mice was much higher than that in control mice(P＜0.01)The level of ankyrin G and MAP2 in the hippocampus of venlafaxine cKO mice were much higher than those of control mice(P＜0.05).Conclusions Venlafaxine alleviates the depression symptoms caused by knocking down Ank3.The mechanism of depression treatment by venlafaxine is potentially associated with levita-ting ankyrin G level in prefrontal cortex and hippocampus.

Objective To explore the relationship between blood pressure variability and different types of stroke.Methods One hundred and twenty-five ischemic stroke subjects,78 hemorrhagic stroke patients and 66 non-stroke individuals,collected in our hospital from January 1,2013 to December 31,2015,were enrolled according to the inclusion and exclusion criteria.All indicators of blood pressure variability were calculated from 24 h ambulatory blood pressure,including nighttime blood pressure fall (BPF),moming surge in blood pressure (MBPS),standard deviation (SD),weighted SD (wSD),and coefficient of variation (CV).Results As compared with non-stroke subjects,ischemic and hemorrhagic stroke individuals had significantly increased systolic MBPS (P＜0.05),and the systolic MBPS of ischemic stroke group was significantly lower than those of hemorrhagic stroke group (P＜0.05).CV,wSD and BPF of systolic blood pressure (SBP) in the ischemic and hemorrhagic groups were significantly higher than those in the non-stroke subjects (P＜0.05);CV and SBPF of diastolic blood pressure in hemorrhagic group were significantly different as compared those in the non-stroke subjects (P＜0.05).The factors which were significantly associated with ischemic stroke in Logistic regression,included high-density lipoprotein,nighttime SBP,wSD of SBP,CV of SBP,while smoking,nighttime SBP,wSD of SBP,MBPS,and CV of SBP were observed as important influence factors for hemorrhagic stoke patients.Conclusion Whether ischemic stroke,or hemorrhagic stroke are associated with short-term blood pressure variability.