Cytochrome P450 family 3 subfamily A(CYP3A),a major member of cytochrome P450(CYP)family,is one of the most important drug metabolizing enzymes in human.CYP3A includes 4 gene subtypes(CYP3A4,CYP3A5,CYP3A7,and CYP3A43),which is involved in 60%of drug metabolism in the human.It is not only widely distributed in normal tissues,but also significantly overexpressed in various tumor tissues.Recently,CYP3A has attracted great attention due to its involvement in the progression from chronic atrophic gastritis to gastric cancer,as well as the differential metabolism and resistance of chemotherapeutic drugs.Targeting CYP3A gene mediated-prodrug provides new ideas for the treatment of gastric cancer and is expected to become a new target for the diagnosis and treatment of gastric cancer.

Objective:To study the protective effect of Ophiopogonin D on lipopolysaccharide (LPS)-induced mouse macrophage RAW264.7 and its related mechanism.Methods:Mouse macrophage RAW264.7 cells were cultured and divided into normal control group, model group and Ophiopogonin D pretreatment group according to random number table method. The activity of Ophiopogonin D on RAW264.7 cells was detected by CCK-8 method; the protein levels of TNF-α, IL-1β, IL-6, reactive oxygen species (ROS), MDA and SOD were detected by ELISA; the protein expression of NF-κB, TLR4, NF-E2-related factor2 (Nrf2) and heme oxygenase-1 (HO-1) were detected by Western Blot.Results:Compared with model group, the levels of TNF-α, IL-1β, IL-6, ROS and MDA in cell supernatant of Ophiopogonin D group were decreased ( P<0.05), and the level of SOD was increased ( P<0.05). The protein expressions of NF-κB (0.76±0.10 vs. 2.26±0.17) and TLR4 (0.98±0.09 vs. 1.74±0.19) significantly decreased ( P<0.05). The protein expressions of Nrf2 (0.85±0.03 vs. 0.54±0.03) and HO-1 (0.97±0.11 vs. 0.37±0.04) significantly increased ( P<0.05). Conclusion:Ophiopogonin D may reduce inflammatory response by reducing TLR4/NF-κB pathway, and activate Nrf2/HO-1 pathway to reduce oxidative damage and play a protective role.

The widespread of tigecycline resistance gene tet(X4) has a serious impact on the clinical efficacy of tigecycline. The development of effective antibiotic adjuvants to combat the looming tigecycline resistance is needed. The synergistic activity between the natural compound β-thujaplicin and tigecycline in vitro was determined by the checkerboard broth microdilution assay and time-dependent killing curve. The mechanism underlining the synergistic effect between β-thujaplicin and tigecycline against tet(X4)-positive Escherichia coli was investigated by determining cell membrane permeability, bacterial intracellular reactive oxygen species (ROS) content, iron content, and tigecycline content. β-thujaplicin exhibited potentiation effect on tigecycline against tet(X4)-positive E. coli in vitro, and presented no significant hemolysis and cytotoxicity within the range of antibacterial concentrations. Mechanistic studies demonstrated that β-thujaplicin significantly increased the permeability of bacterial cell membranes, chelated bacterial intracellular iron, disrupted the iron homeostasis and significantly increased intracellular ROS level. The synergistic effect of β-thujaplicin and tigecycline was identified to be related to interfere with bacterial iron metabolism and facilitate bacterial cell membrane permeability. Our studies provided theoretical and practical data for the application of combined β-thujaplicin with tigecycline in the treatment of tet(X4)-positive E. coli infection.
Humans ; Tigecycline/pharmacology* ; Escherichia coli/metabolism* ; Reactive Oxygen Species/therapeutic use* ; Plasmids ; Anti-Bacterial Agents/metabolism* ; Escherichia coli Infections/microbiology* ; Bacteria/genetics* ; Microbial Sensitivity Tests

Objective:To explore the moderating and mediating effects of psychological capital in the associations of job monotony and depressive symptoms.Methods:A convenient sampling method was used to sample 1 473 workers from a natural gas field in southwest China in October 2018.The sociodemographic characteristics, job monotony scale, PsyCap questionnaire and center of epidemiological survey, depression scale(CES-D) were conducted.Data were analyzed by SPSS 22.0 and Process v3.2 softwares.The statistical methods included t-test, one-way ANOVA, χ2 test, partial correlation analysis, multivariate regression analysis or Bootstrap method. Results:A total of 323 workers with depressive symptoms were detected, with a detection rate of 21.9%(323/1 473). The correlation analysis results showed that job monotony (13.67±1.38) was negatively correlated with self-efficacy (25.81±4.56), hope(25.91±4.55), resilience (26.80±3.80) and optimism (24.56±3.17) ( r=-0.26, -0.38, -0.36, -0.42 respectively, all P<0.01), and positively correlated with depressive symptoms ( r=0.50, P<0.01). Self-efficacy ( r=-0.38, P<0.05), hope ( r=-0.44, P<0.05), resilience ( r=-0.43, P<0.01) and optimism ( r=-0.47, P<0.01) were negatively correlated with depressive symptoms.The multivariate regression analysis results showed that self-efficacy and optimism had moderating effects on the associations of job monotony and depressive symptoms (both P<0.05), while hope and resilience had no moderating effects on the associations of job monotony and depressive symptoms (both P>0.05). The mediating effect test results showed that self-efficacy, hope, resilience and optimism played partial mediating roles in the associations of job monotony and depressive symptoms (all P<0.01), and the mediating effects were 0.139, 0.304, 0.232 and 0.339 respectively, and the proportion of mediating effect value to total effect was 12.2%, 23.3%, 18.8% and 25.3% respectively. Conclusion:Self-efficacy and optimism have moderating and mediating effects on the associations of job monotony and depressive symptoms, while hope and resilience have only mediating effects on the relationship between job monotony and depressive symptoms.

Objective:To explore the incidence of vascular leakage after acute hemodilution in patients with traumatic orthopedics by using 6% hydroxyethyl starch 130/0.4 (HES).Methods:Using prospective cohort study method, 48 orthopedic trauma patients in in Yantaishan Hospital from June 2018 to December 2018 were selected as the subjects of observation.The American Society of anesthesiologists (ASA) grade was divided into grade I-III.According to the degree of trauma, they were divided into two groups: general orthopedic patients group (24 cases) and severe trauma orthopedic patients group (24 cases). According to the formula of blood volume, the blood volume of the patients in the two groups was calculated.After intubation, 10% of the blood volume of HES was infused intravenously at the rate of 0.5 ml/(kg·min) for acute hemodilution.Plasma colloidal osmolality and hemoglobin were measured immediately before acute hemodilution (T0), 15 minutes (T1) and 30 minutes (T2) after acute hemodilution.The concentrations of HES in T1 and T2 plasma were measured.The urine volume from the beginning of infusion to 30 minutes after the end of infusion was saved.The urine volume and hes concentration were measured to calculate the urine hes content.Results:The amount of HES input was the same in the general orthopedic patients group and the severe trauma orthopedic patients group, which were (7.71±0.3) ml/kg and (7.70±0.2) ml/kg, and the expansion ratio was about 100%.Compared with T0, plasma colloid osmotic pressure at T1 and T2 were (27.9±1.5) mmHg(1 mmHg=0.133 kPa)) and (27.7±1.5) mmHg in the general traumatic orthopedics patients, which was higher than T0((26.5±1.5) mmHg, P<0.05). There was no significant difference of COP at T1 and T2 ((27.0±1.6) mmHg and(26.9±1.5) mmHg) compared with T0((26.3±1.7) mmHg, P>0.05) in the severe trauma orthopedic patients). The concentration of plasma HES in the severe trauma orthopedic patients ((6.8±0.6) g/L and (5.8±0.5) g/L) was lower than in the general traumatic orthopedics patients ((7.7±0.5) g/L and (7.1±0.5) g/L, t=5.660 and 6.755, all P<0.05) at T1 and T2.There was no significant difference of the urine HES content ((29.0±3.5 ) mg vs.(28.4±3.3) mg, t=0.61, P>0.05 )between the two groups after infusion. Conclusion:The ratio of acute hemodilution and volume expansion of HES was the same in the two groups.The changes of plasma colloid osmotic pressure and HES concentration were lower in patients with severe trauma orthopedics, and there was more obvious extravascular leakage in patients with severe trauma orthopedics.

Objective:To explore the relationship between sleep quality and occupational stress in field gas recovery workers.Methods:In October 2018, cluster sampling method was adopted to conduct cross-sectional survey on 1726 field workers in a gas production oilfield. The individual characteristics, occupational stress factors, stress regulation factors, stress response and sleep quality, social support and coping strategies were evaluated by occupational stress measurement tools and job content questionnaire. Mann Whitney U test and Kruskal Wallis H test were used to compare sleep quality scores between the groups. Spearman rank correlation analysis was used to analyze the correlation between sleep quality and occupational stress, and logistic regression analysis was used to analyze multiple factors. Results:There were significant differences in sleep quality scores among different positions, gender, marital status, age, length of service, smoking and drinking ( P<0.05) . There were no significant differences in sleep quality scores between different education levels and work shift groups ( P>0.05) . Spearman rank correlation analysis showed that sleep quality score was negatively correlated with job satisfaction, reward, job stability, promotion opportunity, positive emotion, respect, self-esteem, control strategy, support strategy and self-efficacy score ( r s=-0.361, -0.311, -0.238, -0.261, -0.248, -0.212, -0.139, -0.188, -0.152, -0.226, P<0.01) , and was positively correlated with social support, giving, daily tension, negative emotion, work monotony and depression symptom ( r s=0.312, 0.279, 0.547, 0.493, 0.429, 0.599, P<0.01) . Compared with the high sleep quality score group, the middle and low sleep quality score groups had lower giving, work monotony, daily tension, depressive symptoms, negative emotions and social support ( P<0.01) , while the scores of respect, reward, job satisfaction, positive emotion, self-efficacy, job stability, promotion opportunity, control strategy and support strategy were higher ( P<0.01) . Multiple depressive symptoms, high daily tension, high negative emotion and high work monotony were the risk factors for sleep disorders ( OR=3.417, 2.659, 2.913, 1.543) . Conclusion:Depressive symptoms, daily tension and negative emotion have great influence on sleep quality of field gas recovery workers.

Objective:To investigate the level of social support and its correlation with occupational stress among gas production workers in the field.Methods:In October 2018, the cluster sampling method was used to perform a cross-sectional survey for 1726 gas production workers in the field, and related data of these workers were collected, including age, education level, marital status, level of social support, and related factors for occupational stress. A Spearman's rank correlation analysis was used to investigate the correlation between social support and occupational stress, and the levels of occupational stress-related factors were compared between the groups with different social support scores.Results:The gas production workers in the field had a median (25th percentile, 75th percentile) social support score of 24.00 (19.00, 28.00) , and there was a significant difference in social support score between the workers with different posts or work shifts ( P<0.01) . Social support score was positively correlated with effort, daily stress, negative emotion, and job routinization ( P<0.05) and was negatively correlated with job satisfaction, reward, working stability, and promotion opportunity ( P<0.05) . The group with a high social support score had significantly higher scores of effort, job routinization, sleep disorders, and daily stress than the other two groups ( P<0.01) , and the group with a low social support score had significantly higher scores of reward, self-efficacy, positive affection, and job satisfaction than the other two groups ( P<0.01) . Conclusion:High-level social support plays an important role in alleviating occupational stress and protecting mental health among gas production workers in the field.

Objective:To explore the correlation of job monotony and social support on daily hassle.Methods:A convenient sampling survey of demographics characteristics, job monotony, social support and daily hassle was conducted on 1 466 natural gas field workers by questionnaire in October; 2018. Multivariate logistic regression model was used to analyze the correlation of job monotony and social support on daily hassle.Results:The age of 1 466 subjects was (44.47±8.0) years and the length of service was (20.46±5.77) years. There were 258(17.6%), 173(11.8%), 799(54.5%), 120(8.2%) and 116(7.9%) subjects from production and marketing department, processing plant, branch, maintenance department and emergency center respectively. There were 374(25.5%) and 1 092(74.5%) subjects with high and low level of job monotony, and 398(27.1%), 648(44.2%) and 420(28.6%) subjects with high, middle and low level of social support. There were 464(31.7%) and 1 002(68.3%) subjects with high and low daily tension respectively. Compared with the low level of job monotony, the high level of job monotony had a higher risk of daily hassle( OR=4.83, P<0.001), and compared with the high level of social support, the middle and low level of social support had a higher risk of daily hassle( OR=1.25, 2.24, P<0.05). Conclusion:Job monotony and social support were associated with daily hassle.

Objective:To explore the interaction between shift work and psychological capital on abnormal glucose and lipid metabolism.Methods:A convenient sampling survey of demographics characteristics, shift work and psychological capital was conducted on 1 415 natural gas field workers by questionnaire in October 2018,and their physiological and biochemical indexes were measured according to standard norms. Multivariate logistic regression model was used to analyze the interaction between shift work and psychological capital on abnormal glucose and lipid metabolism.Results:For 1 415 subjectsthe prevalence of abnormal blood glucose was 21.2%, the prevalence of diabetes was 8.3%.The prevalence of abnormal total cholesterol was 40.4%, the prevalence of hypercholesterolemia was 11.3%.The prevalence of abnormal triglyceride was 41.6%, the prevalence of hypertriglyceridemia was 24.7%.The detection rate of Low-density Lipoprotein was 17.3%, the detection rate of Low-density Lipoprotein was 4.0%, and the detection rate of high-density Lipoprotein was 1.3%. Multiple logistic regression model analysis showed that shift work, the low level of self-efficacy and the low level of optimism was positively associated with abnormal blood glucose, respectively ( P<0.05). Shift work was positively associated with abnormal triglyceride ( P<0.05). However, there was no interaction between shift work, low self-efficacy, low hope, low resilience, and low optimism on abnormal glucose and lipid metabolism. Conclusion:Shift work was a risk factor of abnormal blood glucose and triglyceride, self-efficacy and optimism were protective factors of abnormal blood glucose. There was no multiplicative interaction between shift work and psychological capital on abnormal glucose and lipid metabolism in the study population.

Objective:To explore the relationship between sleep quality and occupational stress in field gas recovery workers.Methods:In October 2018, cluster sampling method was adopted to conduct cross-sectional survey on 1726 field workers in a gas production oilfield. The individual characteristics, occupational stress factors, stress regulation factors, stress response and sleep quality, social support and coping strategies were evaluated by occupational stress measurement tools and job content questionnaire. Mann Whitney U test and Kruskal Wallis H test were used to compare sleep quality scores between the groups. Spearman rank correlation analysis was used to analyze the correlation between sleep quality and occupational stress, and logistic regression analysis was used to analyze multiple factors. Results:There were significant differences in sleep quality scores among different positions, gender, marital status, age, length of service, smoking and drinking ( P<0.05) . There were no significant differences in sleep quality scores between different education levels and work shift groups ( P>0.05) . Spearman rank correlation analysis showed that sleep quality score was negatively correlated with job satisfaction, reward, job stability, promotion opportunity, positive emotion, respect, self-esteem, control strategy, support strategy and self-efficacy score ( r s=-0.361, -0.311, -0.238, -0.261, -0.248, -0.212, -0.139, -0.188, -0.152, -0.226, P<0.01) , and was positively correlated with social support, giving, daily tension, negative emotion, work monotony and depression symptom ( r s=0.312, 0.279, 0.547, 0.493, 0.429, 0.599, P<0.01) . Compared with the high sleep quality score group, the middle and low sleep quality score groups had lower giving, work monotony, daily tension, depressive symptoms, negative emotions and social support ( P<0.01) , while the scores of respect, reward, job satisfaction, positive emotion, self-efficacy, job stability, promotion opportunity, control strategy and support strategy were higher ( P<0.01) . Multiple depressive symptoms, high daily tension, high negative emotion and high work monotony were the risk factors for sleep disorders ( OR=3.417, 2.659, 2.913, 1.543) . Conclusion:Depressive symptoms, daily tension and negative emotion have great influence on sleep quality of field gas recovery workers.