In order to investigate the apoptosis triggered by porcine circovirus type 2 (PCV2) in lymphocytes and the underlying mechanism, the levels of apoptosis and the expression levels of miRNA were examined by flow cytometry, Western blotting and real-time PCR (qPCR). The mimics or inhibitors of miR-125a-5p, an apoptosis-related miRNA, were transfected into PK-15 cells, and the apoptosis rate was examined upon overexpression or inhibition of mir-125a-5p. The target gene of mir-125a-5p was predicted by bioinformatics method, and the regulation of mir-125a-5p on the target gene was analyzed by luciferase reporter assay. The expressions of Bcl-2, Bax, cytochrome C and caspase-3 were detected by Western blotting. The results showed that exosomes secreted by PK-15 cells infected with PCV2 significantly increased the lymphocyte apoptosis rate, which was dose-dependent in certain concentration range. The expression of miR-125a-5p was dramatically increased. The apoptosis rate was increased significantly in the cells transfected with miR-125a-5p. It was predicted that there were binding sites of miR-125a-5p at Bcl-2 3'UTR by TargetScan. The luciferase activity of wild-type pmir-Bcl-2 3'UTR was inhibited significantly by miR-125a-5p mimics, but that of mutant pmir-Bcl-2 3'UTR was not changed. By Western blotting, Bcl-2 was reduced significantly, while Bax, cytochrome C and caspase-3 increased significantly, and the ratio of Bcl-2/Bax was significantly decreased. These results showed that PCV2 up-regulated the expression of miR-125a-5p through exosomes, then inhibited the expression of Bcl-2 at both mRNA and protein level, activated mitochondrial apoptosis pathway and induced apoptosis in lymphocytes.
3' Untranslated Regions ; Animals ; Apoptosis/genetics* ; Caspase 3/genetics* ; Cell Proliferation/genetics* ; Circovirus/genetics* ; Cytochromes c/genetics* ; Luciferases/genetics* ; Lymphocytes/metabolism* ; MicroRNAs/metabolism* ; Swine ; bcl-2-Associated X Protein/genetics*

Objective:To investigate the level of rumination and its influential factors among Chinese psychiatry nurses who go through work place violence.Methods:In this study, 150 psychiatry nurses were recruited from Affiliated Brain Hospital of Guangzhou Medical University (specialize in psychiatry), via the combination of convenient sampling and snowball sampling. Chinese Event-related Rumination Inventory (C-ERRI) was applied in the survey.Results:The total score of C-ERRI was (22.11±9.62) points, and the scores of intrusive rumination subscale and deliberate rumination subscale were (12.99±5.58) and (9.12±6.01) points, respectively. Multiple Linear Regression Analysis show that assault frequency in recent year ( B=-3.195, P<0.01) and whether got injury in the recent assault ( B=8.591, P<0.01) were predictors of deliberate rumination, which account for 26.8% variance of the equation. Gender (male) ( B=-2.415, P<0.01), Injury frequency in recent year ( B=2.864, P<0.01) and whether got injury in the recent assault ( B=8.949, P<0.01) were predictors of intrusive rumination. They account for 36.0% variance of the equation. Conclusion:In this study, the level of rumination among Chinese psychiatry nurses was low. Their rumination style was deliberate rumination.

Objective:To explore problems and self-experience of the newly graduated recruited nurses in the process of work and to provide a basis for formulating solutions to allow newly graduated recruited nurses to adapt to clinical work as soon as possible.Methods:The phenomenological method of qualitative study was used to interview 25 newly recruited nurses in a ClassⅢ Grade A hospital in Guangzhou from 2018 to 2019.Results:By sorting out, refining and analyzing the interview data, three concepts related to professional confusion, career planning limitation and emphasis on professional acquisition were extracted.Conclusions:Professional confusion is common in newly recruited nurses in department of psychiatry. Medical staff should pay attention to the psychological status and self-experience of newly recruited nurses in department of psychiatry, provide necessary help and support and reduce the negative experience of nurses so as to make them better adapt to clinical work.

Objective To understand psychiatry hospital nurse' s self-compassion situation, explore the influencing factors, for nursing managers to know about the clinical nurses psychological health and to provide a reference data of intervention to improve the level of self-compassion. Methods A total of 381 clinical nurses from the Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital) completed the survey using the questionnaire including the Self-Compassion Scale (SCS) and general information questionnaire. The influence factors were analyzed by chi-square test and Logistic regression analysis. Results The total score of SCS was (85.43 ± 10.23) points in 381 clinical nurses with the medium level, which was less than that of other nurse group (109.21±9.76) points, and there was significant difference(t=-45.388, P < 0.01).The Logistic regression analysis showed that female and working in the psychiatric ward were the risk factors of self compassion(OR=1.772, 1.995, P<0.05 or 0.01). While on the night shift was the protective factor(OR=0.536, P < 0.01). Conclusions Psychiatric hospital nurse' s self-compassion is at medium level. When the nurses cope with the negative events may lack adjustment method. Nursing managers should pay attention to train the ability of the nurse individual self-compassion, targeted to carry out active intervention measures.

Objective To explore the demands of schizophrenics and their primary caregivers for knowledge of mental health during rehabilitation period,so as to provide some references for improving their mental health knowledge.Methods Using the convenience sampling method,49 schizophrenics and 59 primary caregivers completed the general information questionnaire and needs for knowledge of mental health questionnaire.Results The total score of demands for knowledge of mental health of schizophrenics was (55.83±19.29),while the total score of caregivers was (64.88±19.83)(t=-2.376,P=0.019). Conclusions Demands for mental health knowledge of schizophrenics and their primary caregivers are different. Implementation of health education programs should be targeted and individualized.

Objective To investigate the influence of stigma on QOL of patients with mental disorders.Methods By using Link stigma series scale and schizophrenic quality of life scale,we assessed and analyzed 406 psychiatric patients′stigma and QOL, as well as the correlation and influence between them.Results The score of perceived devaluation-discrimination dimension of the stigma scale had positive correlation with total score of QOL,score of psycho-social dimension and score of motivation and energy dimension (P <0.05).The scores of challenge dimension and separation dimension in the stigma scale had positive correlation with motivation and energy dimension of the QOL scale (P <0.05).Regression analysis showed that stigma did not have influence on QOL of patients with mental disorders.Conclusion Stigma in psychiatric patients has no significant influence on QOL,but correla-tion relationship exists between them.The higher score of perceived devaluation-discrimination dimension,challenge dimension and separation dimension of stigma scale,the poor QOL the patient would have.

Objective To investigate needs of caregivers of patients with schizophrenia for mental health knowledge and provide the scientific evidence for formulating the general intervention to improve the caregivers′mental health knowledge and skills . Methods Sixty-five caregivers of the patients with schizophrenia participated in the survey by a general state questionnaire and the requirement to mental health knowledge and skill questionnaire using the convenience sampling method. The survey included mental illness knowledge drug, knowledge rehabilitative therapy and self-adjustment. Results Among all the items of the questionnaire, the 3 items they needed most was mental illness knowledge, the preventing method and the symptoms of the recurrent diseases. The 3 items they needed most in respect of drug knowledge were what examinations are needed when taking special drugs, the adverse reactions of the antipsychotic drugs and the importance of persisting in taking drugs. The 3 they needed most in respect of rehabilitative therapy were the relationship between psychology and disease rehabilitation, the role of mental treatment and the role of work-entertainment therapy. The 3 items they needed most in respect of self-adjustment included sleep adjustment, self-adjustment of affects and self-management of daily life and instructions on dieting. Conclusions The caregivers′mental health knowledge and skills are not enough. Therefore, their mental health knowledge and skills should be strengthened so that they can take better care of the patients to realize comprehensive rehabilitation of patients.

Objective To explore the methods and effectiveness of using nurses as standardized patient ( NSP) in junior nurses′hanging and choking rescue care training. Methods This study selected four nurses as NSPs, based on Huaxi Hospital′s NSP screening criteria. A total of 60 nurses whose psychiatric nursing age were beyond three years were randomly divided into experimental group and control group, with 30 members each. The nurses in the experimental group were treated with NSP method, while the control group was trained in the regular way. After that, the effects of training were evaluated by Dundee Ready Education Environment Measure ( DREEM ) . The theory and clinical skills examination and training satisfaction were surveyed. Results The theory test scores of the two groups after training were significantly higher than those before training (P<0. 05), and the scores of the experimental group was higher than that of the control group, but there was no significant difference (P>0. 05). The total score of DREEM, and the perception of learning, course organizers and academic self-perception score of DREEM of the experimental group were significantly higher than those of the control group (t=7. 086, 9. 443, 10. 462, 8. 620, respectively;P<0. 05). The score of choking rescue skills of the experimental group was significantly higher than that of the control group ( t=2. 759,P<0. 05). But the score of hanging rescue skills of the experimental group was not significantly higher than that of the control group (P>0. 05). There was no significant difference in the satisfaction between the two groups (P>0. 05). Conclusions The application of NSP in hanging and choking rescue care training can improve the teaching environment, increase nurses′choking rescue care skills, but no difference is found in the theory examination and hanging rescue care skills between the two groups.

Objective To discuss the improvement of drug attitude and its influence factors in Schizophrenia inpatients. Methods A total of 185 schizophrenic inpatients were assessed the quality of life and drug attitude, and improvement of them at their admission and before their discharge by life satisfactions rating scales ( SQLS ) and drug attitude inventory ( DAI ) respectively. The Spearman correlation analysis and regression analysis were used to analyze the relationship among the differential value of DAI and SQLS, and demographic data. Results The SOLS scores of all patients dropped down before discharge comparing with scores of admission, in which social psychological dimension was ( 28. 7 ± 17. 0 ) score, dynamic energy dimension (44. 8 ± 14. 5) score, side effect symptom dimension (19. 2 ± 18. 7); the score of DAI increase to (3. 5 ± 4. 6) (t = -5. 831, -12. 112, -7. 373, 9. 875;P <0. 01). The correlation analysis presented gender, each dimension of SQLS improvement had bound with DAI improvement; liner regression analysis revealed that the improvement of dynamic energy and side effects symptom impacted on the improvement ( P<0. 05). Conclusions The improvement of quality of life in schizophrenic inpatients influences the improvement of attitude to taking medication, especially the more improvement of dynamic energy level and side effects symptom. The more quality of life improves, the more improvement of taking medication attitude.

Objective To investigate the state of perceived stigma,self-efficacy and quality of life of patients with mental disorders,and analyze the relationship among these variables,and explore the mediating effect of self-efficacy between the perceived stigma and quality of life.Methods Totally 406 patients with mental disorders were assessed with the Perceived Devaluation-Discrimination Scale (PDDS),Self-efficacy Scale (SES) and Schizophrenia Quality of Life Scale (SQOLS).Results The average score of PDDS was (2.44±0.36).The average score of GSES was (25.75±7.31).The perceived stigma,self-efficacy and quality of life were significantly correlated with each other.The self-efficacy played a mediating role between perceived stigma and quality of life.Conclusions The self-efficacy is the intermediary variable of perceived stigma and QOL of patients with mental disorders.