Background: Tumor spread through air spaces (STAS) is a recently discovered risk factor for lung adenocarcinoma (LUAD). The aim of this study was to investigate specific genetic alterations and anticancer immune responses related to STAS. By using a machine learning algorithm and drug screening in lung cancer cell lines, we analyzed the effect of Janus kinase 2 (JAK2) on the survival of patients with LUAD and possible drug candidates. Methods: This study included 566 patients with LUAD corresponding to clinicopathological and genetic data. For analyses of LUAD, we applied gene set enrichment analysis (GSEA), in silico cytometry, pathway network analysis, in vitro drug screening, and gradient boosting machine (GBM) analysis. Results: The patients with STAS had a shorter survival time than those without STAS (P < 0.001). We detected gene set-related downregulation of JAK2 associated with STAS using GSEA. Low JAK2 expression was related to poor prognosis and a low CD8+ T-cell fraction. In GBM, JAK2 showed improved survival prediction performance when it was added to other parameters (T stage, N stage, lymphovascular invasion, pleural invasion, tumor size). In drug screening, mirin, CCT007093, dihydroretenone, and ABT737 suppressed the growth of lung cancer cell lines with low JAK2 expression. Conclusion In LUAD, low JAK2 expression linked to the presence of STAS might serve as an unfavorable prognostic factor. A relationship between JAK2 and CD8+ T cells suggests that STAS is indirectly related to the anticancer immune response. These results may contribute to the design of future experimental research and drug development programs for LUAD with STAS.

Acute renal failure with severe loin pain and patch renal ischemia after anaerobic exercise (ALPE) is a rare cause of exercise-induced acute kidney injury. Some ALPE patients also have renal hypouricemia. Mutations in the SCL22A12 gene are among the major factors of hypouricemia. Education for the prevention of relapse and genetic counseling should be recommended to ALPE patients with renal hypouricemia. This paper reports a 25-year-old man who showed recurrent exercise-induced ARF and renal hypouricemia with R90H mutation in his SCL22A12 gene.
Acute Kidney Injury ; Genetic Counseling ; Humans ; Ischemia ; Recurrence ; Renal Tubular Transport, Inborn Errors ; Urinary Calculi

Conventional open discectomy is considered as the standard treatment for cervical disc herniation.However, open discectomy does not always yield a successful result.Nucleoplasty is a newly developed minimal invasive spinal surgery that uses the Coblation technique.The concept of Coblation involves the use of radiofrequency energy to ablate the nucleus pulposus tissue in a controlled approach leading to reduction of pressure on the nerve roots.Inclusion criteria for candidates for nucleoplasty include complaints of symptoms related to a contained herniated disc or focal protrusion.We describe here our experience with the use of nucleoplasty for a 49-year-old male patient with a cervical disc extrusion.Percutaneous decompression with nucleoplasty using the Coblation technique for the treatment of cervical disc extrusion was successfully performed with an excellent result.
Decompression ; Diskectomy ; Humans ; Intervertebral Disc Displacement ; Male ; Middle Aged