Pesticides and chemical fertilizers are widely used in agriculture to increase crop productivity among farmers.However, exposure to pesticides will give potential risk to human health. The aim of this study was to analyze thefrequency of micronucleus (MN) and binucleus (BNu) formation in buccal cells from farmers who were exposedto pesticides using the MN assay. Buccal swabs were collected from the farmers in Tanjung Karang (n = 32) andKelantan (n = 43) using wooden tongue depressor. A structured questionnaire was used to obtain demographic dataof the farmers. Cytogenetic analysis was carried out by Acridin Orange (AO) staining 0.0025% (w/v). The frequencyof MN and BNu as the biomarkers for cytogenetic damage was observed by using a fluorescence microscope.Comparison of frequency of MN and BNu is conducted in two areas namely Tanjung Karang, Selangor and Kelantanbecause of the agricultural activity and the type of pesticides used are different. Results showed that the frequencies of bothMN and BNu among farmers in Tanjung Karang were significantly higher (p < 0.05) compared to farmers in Kelantan.Meanwhile, for the socio-demographic factors (age, smoking status, working period), MN and BNu frequencies amongfarmers in Tanjung Karang were also significantly higher (p < 0.05) as compared to farmers in Kelantan. While in theaspect of pesticide exposure, the frequencies of MN and BNu showed no significant difference between the frequency ofpesticide spraying (p > 0.05) and the practices of PPE (Personal Protective Equipment) (p > 0.05). This may suggeststhat cytogenetic changes were not influenced by these factors. In addition, correlation study shows positive correlationbetween the frequency of MN with the pesticide exposure of farmers in Tanjung Karang (p > 0.05, r = 0.015) and Kelantan(p > 0.05, r = 0.0158). Besides, the frequency of BNu also has a positive correlation with the pesticide exposure amongfarmers in Tanjung Karang (p > 0.05, r = 0.036) and farmers in Kelantan (p > 0.05, r = 0.013). Hence, this present study demonstrated that exposure to pesticides increasedthe formation of MN and BNu among farmers and theprolonged use of pesticides may induce genotoxicity andDNA damage to human.

Postprandial hyperglycemia is independently related to cardiovascular disease. Garlic, ginger, lemon, honey and applecider vinegar are known to have anti-glycemic properties. However, the effectiveness of combination of these naturalproducts on reducing postprandial glycemia is uncertain. The aim of the present study was to investigate the glucoseloweringeffect of a novel mixture consisting of apple cider vinegar, garlic, ginger, lemon, and honey; alone and incombination with exercise in response to a high-carbohydrate meal in non-diabetic individuals. Ten, female subjects (meanage: 25 ± 2.67 years, mean BMI: 22.6 ± 3.5 kg/m2) participated in this randomised, cross-over intervention consisting offour trials: control (CON), mixture only (MIX), exercise only (EX), and exercise + mixture (EX-MIX). All trials involvedconsumption of a high-carbohydrate breakfast, then followed by rest in CON, consumption of natural product mixturein MIX, brisk-walking exercise in EX, and combination of mixture and exercise in EX-MIX. Blood glucose was measuredat fasting, and at 30, 60, 90, 120 minutes post meal. Postprandial glucose response was calculated as area under theglucose curve. Two-way repeated measures ANOVA showed a significant group and time interaction (p < 0.001). Comparedto CON, postprandial glucose responses were 8%, 13% and 15% lower in MIX (p = 0.049), EX (p = 0.001) and EX-MIX(p = 0.005) respectively. Postprandial glucose was 8% lower in EX-MIX compared to MIX (p = 0.002). In conclusion,consuming natural product mixture containing garlic, ginger, lemon, honey and apple cider vinegar reduced postprandialglycemia to a certain extent, however, combining mixture with exercise produced a greater attenuation effect comparedto consuming mixture alone. This finding is indicative of a potential benefit of the novel mixture as a complementarymanagement of hyperglycemia in high-risk individual

Motivation camp is one of the learning interventions to assist underperform students in improving their academicperformances in term of GPA (Grade Point Average). An intervention study was carried out to know the effectiveness ofa motivation camp in improving the academic performances among the biomedical science students at the UniversitiKebangsaan Malaysia, Kuala Lumpur. Total of 44 respondents were involved in this study from year 2 and year 3 and hadbeen categorized into control and intervention groups (n = 22 per group). The intervention group was given motivationaltalks followed by a group discussion and self-reflection. The students in the intervention group were also assigned withacademicians to facilitate and guide them throughout the motivational camp. The students were interested in motivationaltalks activity the most. The average GPA showed a significant improvement after the motivation camp with 3.01 ± 0.30(p < 0.05) for the intervention group. Yet, control group also displayed a significant increment in the average GPA, 3.24± 0.06 (p < 0.05). In conclusion, motivation camp is one of the many learning intervention tools that strategize to helpstudents in improving their academic performances but it should be complemented with other intervention methods.Keyword

Tahfiz education has been well accepted by Muslims in Malaysia. Memorizing the Quran through rote learning can assistin improving the brain ability to process, store information and build memory. In addition, dietary intakes were reportedto contribute in the process of memorizing the Quran and to increase the levels of intelligence (IQ) among huffaz students.This cross-sectional study was conducted to measure and to determine the correlation between IQ and dietary intakeas well as between IQ and the level of trace elements among students in selected tahfiz schools and non-tahfiz school inSelangor. Questionnaires were used to obtain demographic data. Dietary intake was assessed via 3 days dietary record.Levels of trace elements in the nail samples were analyzed by using ICP-MS. Test for intelligence using WASI-II wasconducted to measure the students’ IQ. The results showed that there was a moderate positive correlation between IQ andcarbohydrate (r = 0.425, p < 0.001, a weak positive correlation between IQ and energy (r = 0.260, p < 0.05), vitaminB6 (r = 0.189, p < 0.05), vitamin B12 (r = 0.207, p < 0.05) and vitamin C (r = 0.211, p < 0.05). The levels of traceelements in nail samples showed no correlation with IQ, whereas there were moderate positive correlations between IQand dietary zinc (r = 0.375, p < 0.001) as well as between IQ and dietary iron (r = 0.303, p < 0.001). There was also amoderate positive correlation between IQ and the level of memorization (r = 0.375, p < 0.001). The results of stepwisemultiple linear regression showed that al-Quran memorization influence the level of IQ by 20 %. As a conclusion, dietaryintake of certain nutrients including energy, vitamins and selected trace elements can potentially improve memorizationactivities and also IQ.

Background: Caregivers of children with learning disabilities have been shown to experience increased stress and greater negative caregiving consequences than those with typically developing children. There remains a lack of studies focusing on stress and coping mechanisms among caregivers of a wider age group and diagnosis of individuals with disabilities in Asian countries. The current study examines levels of perceived stress and associated child and caregiver factors among caregivers of children with learning disabilities in the Malaysian context. An additional aim was to determine whether caregiver coping styles may be predictors of perceived stress. Methods: The Malay version of the Perceived Stress Scale with 10 items and the Brief COPE Scale were administered to a sample of 190 Malay caregivers of children with learning disabilities registered with community-based rehabilitation centres in Kelantan, a state in Peninsular Malaysia. Multiple linear regression analysis was applied to determine the predictors of perceived stress. Results: The mean total perceived stress score of caregivers was 16.96 (SD = 4.66). The most frequently used coping styles found among caregivers included religion, acceptance and positive reframing, while substance use and behavioural disengagement were least frequently used. Higher perceived stress was significantly predicted among caregivers with fewer children, frequent use of instrumental support and behavioural disengagement coping, and lack of emotional support and religious coping. Conclusion: Findings indicate that the perceived stress levels among caregivers were significantly predicted by different coping styles. It is vital to help the caregivers improve their good coping styles in order to reduce their stress levels.

This paper investigate the effect of parents’ education background and family monthly income on the Intelligence Quotient (IQ) of students with special needs in Kelantan, Malaysia. This cross-sectional method study was employed multistage random sampling to obtain information drawn from 130 participants from 10 selected school in Kelantan, Malaysia. Questionnaire was used to obtain parents’ education background, family monthly income and demographic variables. Students IQ were assessed using Comprehensive Test for Non-Verbal Intelligence (CTONI 2nd Edition). Data analysis involve independent sample t-test, one-way between group ANOVA, Pearson’s correlation and multiple linear regression. From analysis of IQ score, 63.8% of the special needs students scored very poor IQ, 12.3% scored below average and only 1.5% score average IQ. Significant mean difference were revealed between age group (p = 0.002), parents’ education (p = 0.018) and family monthly income (p < 0.05) on special needs students IQ. Post-hoc tukey shows significance between parents who never went to school, went to secondary school (p = 0.037) and university/college (p = 0.021). In term of family monthly income, significance difference were found between family with low and high monthly income (p < 0.05). Family monthly income (r = 0.393, p < 0.01) showed positive moderate correlation on special needs students IQ. After being forwarded by multiple linear regression, it was found that family monthly income (B = 3.605, p < 0.05) and age group of special needs students (B = 0.879, p = 0.002) were significant predictor for IQ score and explained 22.5% of the variance (R2 = 0.225, F (5,124) = 83.94, p < 0.05. Overally, majority of special needs students in current study have very poor IQ score. Further explanation are discussed on the paper.

Parents or caregivers of children with learning disabilities have been shown to experience increases in stress and greater negative caregiving consequences than those with typically developing children. The current study sought to assess the perceived stress among Malay caregivers of children with learning disabilities in Kelantan. The Malay version of Perceived Stress Scale 10 items was administered to a sample of 40 caregivers of children with learning disabilities who were registered to five Pusat Pemulihan Dalam Komuniti (PDK) in Kelantan. Higher scores indicate higher levels of stress. The caregivers had mean age of 47.68 (SD = 9.18) years old, of whom 90% were fathers or mothers. Ninety percent of them were married, majority were unemployed or housewives and had secondary school education. The majority of children with learning disabilities were males and half of them were Down Syndrome children. The mean total Perceived Stress Scale score of the caregivers was 16.77 (SD = 5.74). There were no significant associations between total perceived stress score and any of the independent variables. The mean total perceived stress score showed that the perceived stress level was in the category of slightly higher than average and health concern level was high, while the average stress level was between score of 12 to 15. In conclusion, this result indicated that the caregivers had slightly higher levels of stress than the average score, and might increase susceptibility to stress-induced illness.
Stress ; Caregivers

Background: Microwave is the most reliable sample digestion method. However, it requires expensive microwave digester automation and has relatively low productivity. In this study, three non-automated digestion methods, i.e. wet acid digestion using nitric acid (HNO3) and hydrogen peroxide (H2O2), wet acid digestion using HNO3, and dry washing, are compared in order to determine the best approach. Methods: Certified reference material IAEA-086 (International Atomic Energy Agency, Austria) and hair and nail samples from 20 female students of Universiti Kebangsaan Malaysia, aged 19 to 30 years, were collected and analysed using the three digestion methods. Results: For hair samples, analysis of variance of repeated measures showed significant differences in the level of all elements (P < 0.001) between the three methods. For nail samples, only the copper (Cu) level showed no significant difference (P = 0.100) between methods. Wet acid digestion using HNO3 and H2O2 showed the best within- and between-run relative standard deviation (RSD) values, with within-run RSD for all elements, except for selenium (Se), < 5%. The between-run precision ranges from 6.14% to 17.96% for hair and from 3.53% to 11.52% for nail samples. Wet acid digestion using HNO3 and H2O2 showed both good accuracy and precision for manganese (Mn) and magnesium (Mg), with percentage recoveries of 110% and 96.9%, respectively. All elements show higher method detection limit (MDL) values than the previous study: 0.05 μg/g Mg for wet acid digestion using HNO3, 0.02 μg/g Se for wet acid digestion using HNO3 and H2O2, and 0.2 μg/g Mg for dry ash method. Conclusion: Wet acid digestion using HNO3 and H2O2 proved to be the best method in terms of precision, accuracy, recovery, and MDL. However, only Mn and Mg showed adequate precision, accuracy, and percentage of recovery.

Chromium is an essential mineral and cofactor for insulin that plays an important role as hormone that helps in the blood sugar regulation. Kelantanese were known as sugar-based food consumer. Farmers have high risk of health problem because of exposure to pesticide and sugar-based food intake can give an effect on the level of chromium. The objective of this research was to study the status of chromium among farmers exposed to pesticides and fertilizer in Kelantan. This was a cross-sectional study that was done at Bachok and Pasir Puteh, Kelantan. Respondent were 113 farmers who had been exposed to pesticides or fertilizer for not less than one year. Subjects were interviewed to obtain information on their demographic data by using validated knowledge, attitude and practice (KAP) questionnaire. Nails and hair chromium levels were analyzed by using acid digestion method and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). Results showed that 81.4% were male while 18.6% were female. Farmer’s chromium level in nails (125.82 ± 47.81 μg/L) and hair (39.63 ± 5.70 μg/L) were very much lower when compared with standard value in nails (6200 μg/L) and hair (100-2500 μg/L). No signifi cantly differences (p > 0.05) were found between chromium level according to gender, age, level of pesticide exposure, dietary chromium intake and blood glucose. Chromium levels in nails and hair of farmers who smoke was signifi cantly lower (p < 0.05) than non-smoking farmers. As a conclusion, the level of chromium among farmers in Bachok and Pasir Puteh, Kelantan are lower than normal reference and farmers should stop smoking because smoking can lower the chromium level

Health awareness promotion among farming communities are important for a sustainable agriculture activities. A crosssectional study was conducted to assess health status among farming communities in Cameron Highlands, Pahang (n = 61) and Bachok and Pasir Puteh, Kelantan (n = 143). Mobile Health Screening Programme composed of assessment of blood glucose, blood cholesterol, haemoglobin, blood pressure, pterygium, lung function and nerve conductive velocity was utilized. Our results indicate that the percentage of Cameron Highland’s farmers with hyperglycaemia, systolic hypertension, diastolic hypertension and anaemia were 8.2%, 14.8%, 11.5% and 8.2%, respectively. However, higher percentage of farmers in Bachok and Pasir Puteh, Kelantan with hyperglycaemia (32.8%), hypercholesterolaemia (83%), anaemia (24.2%) and systolic hypertension (41.9%) were observed. Pterygium was positive for 88.6% of farmers in Cameron Highlands and 94.4% in Bachok and Pasir Puteh. Lung function test shows that 61.7% and 11.4% of farmers in Cameron Highlands had restrictive and obstructive lung, respectively. In Bachok and Pasir Puteh, a total of 19.8%, 55.5% and 23.9% of farmers were found to have obstructive, restrictive and combined obstructive and restrictive lung, respectively. Current Perception Threshold (CPT) value which indicate nerve conductive velocity were signifi cantly increased (p < 0.05) among Cameron Highland’s farmers for both median and peroneal nerve at all frequencies (5 Hz, 250 Hz and 2000 Hz). In Bachok and Pasir Puteh, the values of the CPT for median nerve was signifi cantly increased (p < 0.05) for all frequencies (5, 250 and 2000 Hz). Meanwhile, a signifi cant increased (p < 0.05) was observed for the CPT values for peroneal nerve at the frequencies of 250 and 2000 Hz as compared to control groups. In conclusion, analysis revealed different health problem among the studied farming communities which could be infl uenced by the differences in farming practices. Thus, employed Mobile Health Screening Programme offers a monitoring approach that could highlight the need for suitable health services and awareness programmes for different farming communities