ObjectiveTo analyze the epidemic characteristics of foodborne disease outbreaks in Mengla County， Yunnan Province， so as to provide basis for formulating corresponding prevention and control measures. MethodsThe data of foodborne disease outbreaks in Mengla County， Yunnan Province from 2017 to 2021 in the national "foodborne disease outbreak monitoring system" were collected. The time， population， region， place， pathogenic factors and inducing links of the events were statistically analyzed. ResultsFrom 2017 to 2021， a total of 68 incidents were reported， including 526 cases and 5 deaths. The total incidence rate was 23.40% and the case fatality rate was 0.95%. The peak period was from May to July， with the largest number of reported events in July. The reporting areas were mainly Mengla Town （35.29%）， Guanlei Town （11.76%） and Mengpeng Town （10.29%）. The main place of the incident was family （76.47%）. The main pathogenic factors were plant toxins （79.41%）， mainly through the ingestion of wild mushrooms （68.52%）， improper processing of green beans （12.96%） and aconitum （9.26%）. ConclusionAccording to the epidemiological characteristics of Mengla County， we should focus on the public education of health knowledge in key areas and places， especially on the identification of wild mushrooms， to prevent accidental eating. At the same time， we should strengthen the monitoring and risk assessment of foodborne diseases， find potential risks as soon as possible， issue early warning and forecast in time， and constantly improve the clinical treatment ability of poisoned patients.

Intensive Care Unit (ICU) nurses, as the front-line personnel who directly care for critically ill patients, play an important role in clinical antimicrobial stewardship practice. Knowledge and attitudes of ICU nurses involved in antimicrobial stewardship directly affect the implementation effect of antimicrobial stewardship behavior. This paper reviews the current situation and influencing factors of ICU nurses' antimicrobial stewardship knowledge, attitude and practice at home and abroad, and proposes targeted intervention strategies, so as to provide reference and basis for promoting the sustainable development of antimicrobial stewardship in medical institutions in China, related research, education and training.

In coronavirus disease 2019 (COVID-19) epidemic prevention and control, effective control of nosocomial infection and prevention of occupational exposure of first-line medical staff are the requirements of scientific prevention and control, as well as the premise and foundation of scientific treatment of patients. This paper introduces the priority of personnel management of infectious occupational exposure risk at The First Affiliated Hospital of Xi’an Jiaotong University, and analyzes engineering control, management and behavior control, and personal protection. It elaborates on the hospital management methods and proposes scientific preventive measures.

Chronic disease medication adherence is directly related to the health and hospital admission rate of the aged in the community. This article reviews the progress of community interventions on medication adherence for elderly patients with chronic diseases at home and abroad, and systematically introduces the intervention objects, intervention locations, implementers, evaluation indicators, intervention implementation and effects, and cost-benefit evaluations, and puts forward thoughts and suggestions for existing problems so as to provide a reference for future community nursing intervention and research.

OBJECTIVE: To prepare warangalone-loaded thermosensitive liposomes (WLTSL) and evaluate its inhibitory effect on breast cancer cells . METHODS: MTT assay was used to assess the changes in proliferation of 3 breast cancer cell lines (MDA-MB-231, MCF7, and SKBR3) following treatment with warangalone, soy isoflavone and genistein. Colony-forming assay and wound healing assay was used to assess colony forming activity and migration of MDA-MB-231 cells treated with warangalone. The effect of warangalone on the expression of MMP2 and MMP9 in MDA-MB-231 cells was examined with Western blotting. The thermosensitive liposomes (TSL) and WLTSL were prepared using a thin film hydration method, and the morphology, size, encapsulation efficiency and stability of the prepared liposomes were characterized using transmission electron microscopy, dynamic light scattering scanning and UV spectrophotometry. MTT assay was used to examine the inhibitory effect of WLTSL on mouse breast cancer cells (4T1) . RESULTS: Warangalone showed stronger anti-proliferation effects than soy isoflavones and genistein in the 3 human breast cancer cell lines and significantly inhibited colony formation by MDA-MB-231 cells. Treatment with warangalone significantly inhibited migration of the breast cancer cells and down-regulated the cellular expressions of MMP2 and MMP9. The prepared TSL and WLTSL presented with a homogeneous, irregular spherical morphology, with a mean particle size of 56.23±0.61 nm, a polymer dispersity index of 0.241±0.014, a Zeta potential of -40.40±0.46 mV, and an encapsulation efficiency was 87.68±2.41%. WLTSL showed a good stability at 4 ℃ and 37 ℃ and a stronger inhibitory effect than warangalone in 4T1 cells. CONCLUSIONS Warangalone inhibits the proliferation, migration and invasion of breast cancer cells, and the prepared WLTSL possesses good physical properties and strong anti-breast cancer activity.
Animals ; Breast Neoplasms ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Humans ; Isoflavones ; Liposomes ; Mice

Objective? To analyze the risk factors of nosocomial infection in the pediatric ward in a hospital and nursing care for relevant prevention and control. Methods? Totally 1 460 pediatric inpatients admitted in Taizhou First People's Hospital from June 2017 to January 2019 were retrospectively analyzed and divided into the infection group and the control group according to the existence of infection. The children＇s medical records were organized; the risk factors of nosocomial infection in pediatric inpatients were analyzed;and relevant nursing measures were summarized. Results? Nosocomial infection was found in 89 out of the 1 460 children; most children were found with respiratory tract infection and digestive canal infection in the infected children, which accounted for 36 cases (40.4%) and 23 cases (25.8%), respectively; the proportion of children aged ＜3 years, hospitalized for ＞10 days, with malnutrition, invasive operation, receiving antibiotics for ＞10 days, or receiving immunosuppressors or unqualified sterile operation in the infection group was higher than that in the control group, and there was statistically significant difference between the two groups (P＜ 0.05). Logistic regression analysis revealed that nosocomial infection in pediatric inpatients was associated with length of hospital ＞ 10 days, invasive operation, use of antibiotics for ＞ 10 days, use of immunosuppressors and unqualified sterile operation. Conclusions? Pediatric inpatients are prone to nosocomial infection, and the type of infection is mainly respiratory tract infection and digestive canal infection. Relevant nursing measures should be taken based on the factors of infection in order to reduce the incidence rate of nosocomial infection in children.

Objective To explore the clinical effect of Xiyanping injection combined with bifidobacterium in the treatment of infantile virus diarrhea.Methods 80 cases of infantile virus diarrhea were selected,they were randomly divided into control group and research group according to the digital table,40 cases in each group.The control group received intravenous drip ribavirin treatment,the research group received treatment of intravenous drip Xiyanping injection combined with bifidobacterium at the same time.The symptoms disappear time(time of diarrhoea,antifebrile time),length of hospital stay and clinical curative effect were observed and compared between the two groups.Results The indicator of symptoms disappear time (time of diarrhoea,antifebrile time) and length of hospital stay of the research group were (2.19 ±0.43)d,(1.75 ±0.36)d,(4.13 ±0.85)d,respectively,which were shorter than those of the control group [(4.89 ± 0.61) d,(3.68 ± 0.52) d,(7.91 ± 1.13) d],the differences were statistically significant(t =22.89,19.30,16.90,all P ＜ 0.01).The total effective rate of the research group was 97.5％,which was significantly higher than 72.5％ of the control group (x2 =24.5,P ＜ 0.01).Conclusion Xiyanping injection combined with bifidobacterium in the treatment of infantile virus diarrhea has significantly shorter antidiarrheal time,antifebrile time,length of hospital stay than ribavirin,the clinical curative effect is distinct,which is worthy of clinical popularization and application.

Objective To investigate the effect of fatty acid binding protein 4(FABP4)DNA methylation on abnormal lipid metabolism in placental trophoblastic dyslipidemia. Methods Human placental trophoblast cell line(HTR-8)was treated with L-NAME of 100 μmol/L for 48 h. The lipid content in placental trophoblasts was detected by chemical enzyme-colorimetry. The FABP4 DNA methylation level in placenta trophoblasts was detected by nested-touch down methylation specific PCR (NT-MSP). the mRNA and protein expression of DNMT1 and FABP4 were detected by qRT-PCR and Western Blot,respectively,in trophoblast cells. Results The lipid content in trophoblasts significantly increased as compared with the control(P < 0.05). Expression of FABP4 mRNA and protein increased(P < 0.05),while FABP4 methylation level and expression of DNMT1 significantly decreased (P<0.05)after treatment with L-NAME. Conclusions FABP4 DNA methylation is involved in the regulation of lipid metabolism in placental trophoblastic cells of hypertensive disorder complicating pregnancy.

Objective To explore the characteristics of family functioning between parents and adolescent and the association of adolescents' self-esteem,and depression.Methods Data were collected from reports of 257 middle school students and their parents in Shanghai,and by self-evaluation questionnaires including the depression scale,the self-esteem scale,and the family adaptability and cohesion scale.Results ①Adolescent perceived more negative than parents in the family adaptability (69.02 ± 11.49),(70.60± 9.60) and cohesion (43.84 ± 9.25),(46.56±7.86),and the difference in family adaptability were significant (t=4.064,P＜0.01).② Adolescents' self-esteem positively related to their family cohesion(r=0.525) and adaptability (r=0.427,P＜0.01),while depression negatively related to family cohesion(r=-0.565) and adaptability (r=-0.475,P＜0.01).③Students in families with high adolescent-parent perceptual differences in family functioning showed more depressive symptom,lower level of self-esteem than those in low adolescent-parent perceptual differences,and the difference were significant (P＜0.05).Conclusion Adolescents' self-esteem,depression symptoms are significantly related to their familyadaptability and cohesion,the adolescent-parent perceptual differences in family functioning may also affect adolescents' psychological well being.