[Objective]To explore the modern pharmacological mechanisms of Coptidis Rhizoma-Cimicifugae Rhizoma herbal pair in the treatment of recurrent aphthous ulcer(RAU)and analyze the possible traditional Chinese medicine(TCM)therapeutic factors to further guide TCM clinical diagnosis and treatment.[Methods]The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)was used to retrieve drug components and targets.Disease-related targets were obtained from databases such as Online Mendelian Inheritance in Man(OMIM)and Human Genome Annotation Database(GeneCards).The intersection of targets was analyzed using the STRING platform for protein-protein interaction(PPI)analysis.The PPI network was visualized using Cytoscape 3.7.2,and core targets were selected.Gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using the MetaScape database,and a network diagram was constructed.Molecular docking was conducted using AutoDock 4.2.6,and the docking results were visualized using PyMOL software.A rat model of RAU was established by bilateral subcutaneous injection of complete Freund's adjuvant.Real-time quantitative polymerase chain reaction(Real-time qPCR)was used to detect the expression of core target genes in the oral tissues of rats in each group.[Results]A total of 19 active components of Coptidis Rhizoma-Cimicifugae Rhizoma were screened,along with 191 targets.There were 127 intersecting targets between herbs and diseases,and 23 core targets were identified for RAU intervention.Based on Degree,seven key targets were selected,and the core pathway was the signaling pathway associated with interleukin-17(IL-17)and tumor necrosis factor(TNF).The docking results showed that the core active component stigmasterol exhibited high binding activity with each key target.Animal experiments showed that the herbal pair treatment significantly reduced the number of oral ulcers in rats.Compared with blank control group,the expression of core targets in model group,low-dose group,medium-dose group and high-dose group was significantly higher(P＜0.05).The relative mRNA expression and number of oral ulcers in the high-dose group were significantly lower than those in model group,low-dose group and medium-dose group(P＜0.05).[Conclusion]The molecular mechanisms of Coptidis Rhizoma-Cimicifugae Rhizoma herbal pair in the treatment of RAU are related to its anti-inflammatory,oral mucosal protection and immunomodulatory effects.The relevant TCM therapeutic mechanisms involve heat-clearing and detoxifying,promoting blood circulation and removing blood stasis,eliminating toxins,promoting wound healing and other effects.

Objective: To compare the clinical efficacy between endoscopic nipple-sparing mastectomy with immediat reconstruction using prosthesis implantation and open surgery. Methods: Totally 189 early-stage breast cancer patients admitted at Department of Breast and Thyroid Surgery, Southwest Hospital, Third Military Medical University from January 2013 to December 2017 were enrolled. Among them, 104 patients underwent endoscopic nipple sparing mastectomy with immediat reconstruction using prosthesis implantation (endoscopic group), with an age of (41.7±6.1) years (range: 25 to 51 years), and 85 patients underwent traditional open surgery (open group), with an age of (41.6±7.7) years (range: 27 to 65 years). The operative duration, the volume of intraoperative blood loss, the volume of drainage in 3 days after surgery, postoperative complications and patients′ satisfaction of breast reconstruction were compared between the two groups using t test, Mann-Whitney U test, χ² test or non-parametric test. Results: There were no statistically significant differences in postoperative complications, the rates of recurrence and overall survival between the two groups (P>0.05). The operative duration (sentinel lymph node biopsy: (178±80) minutes vs. (198±42) minutes, t=-2.082, P=0.039; axillary lymph node dissection: (204±79) minutes vs. (233±49) minutes, t=-2.952, P=0.004), the volume of drainage in three days postoperative ((183±141)ml vs. (237±104) ml, t=-2.938, P=0.004) in the open group were lower than endoscopic group. The volume of intraoperative blood loss in the endoscopic group was lower than that in the open group ((87±64) ml vs. (62±36) ml, t=3.210, P=0.002). Patients′ satisfaction of breast reconstruction in the endoscopic group was higher than that in the open group. Conclusions Both endoscopic nipple sparing mastectomy with immediat reconstruction using prosthesis implantation and open surgery are safe in oncology. Endoscopic surgery maybe more suitable alternative in breast reconstruction for early-stage breast cancer patients.

Objective To compare the clinical efficacy between endoscopic nipple?sparing mastectomy with immediat reconstruction using prosthesis implantation and open surgery. Methods Totally 189 early?stage breast cancer patients admitted at Department of Breast and Thyroid Surgery, Southwest Hospital, Third Military Medical University from January 2013 to December 2017 were enrolled. Among them, 104 patients underwent endoscopic nipple sparing mastectomy with immediat reconstruction using prosthesis implantation (endoscopic group), with an age of (41.7±6.1) years (range: 25 to 51 years), and 85 patients underwent traditional open surgery (open group), with an age of (41.6±7.7) years (range: 27 to 65 years). The operative duration, the volume of intraoperative blood loss, the volume of drainage in 3 days after surgery, postoperative complications and patients′satisfaction of breast reconstruction were compared between the two groups using t test, Mann?Whitney U test, χ2 test or non?parametric test. Results There were no statistically significant differences in postoperative complications, the rates of recurrence and overall survival between the two groups (P>0.05). The operative duration (sentinel lymph node biopsy: (178± 80) minutes vs. (198±42) minutes, t=-2.082, P=0.039; axillary lymph node dissection: (204±79) minutes vs. (233±49) minutes, t=-2.952, P=0.004), the volume of drainage in three days postoperative ((183±141)ml vs. (237 ± 104) ml, t=-2.938, P=0.004) in the open group were lower than endoscopic group. The volume of intraoperative blood loss in the endoscopic group was lower than that in the open group ((87±64) ml vs. (62± 36) ml, t=3.210, P=0.002). Patients′satisfaction of breast reconstruction in the endoscopic group was higher than that in the open group. Conclusions Both endoscopic nipple sparing mastectomy with immediat reconstruction using prosthesis implantation and open surgery are safe in oncology. Endoscopic surgery maybe more suitable alternative in breast reconstruction for early?stage breast cancer patients.

Objective To compare the clinical efficacy between endoscopic nipple?sparing mastectomy with immediat reconstruction using prosthesis implantation and open surgery. Methods Totally 189 early?stage breast cancer patients admitted at Department of Breast and Thyroid Surgery, Southwest Hospital, Third Military Medical University from January 2013 to December 2017 were enrolled. Among them, 104 patients underwent endoscopic nipple sparing mastectomy with immediat reconstruction using prosthesis implantation (endoscopic group), with an age of (41.7±6.1) years (range: 25 to 51 years), and 85 patients underwent traditional open surgery (open group), with an age of (41.6±7.7) years (range: 27 to 65 years). The operative duration, the volume of intraoperative blood loss, the volume of drainage in 3 days after surgery, postoperative complications and patients′satisfaction of breast reconstruction were compared between the two groups using t test, Mann?Whitney U test, χ2 test or non?parametric test. Results There were no statistically significant differences in postoperative complications, the rates of recurrence and overall survival between the two groups (P>0.05). The operative duration (sentinel lymph node biopsy: (178± 80) minutes vs. (198±42) minutes, t=-2.082, P=0.039; axillary lymph node dissection: (204±79) minutes vs. (233±49) minutes, t=-2.952, P=0.004), the volume of drainage in three days postoperative ((183±141)ml vs. (237 ± 104) ml, t=-2.938, P=0.004) in the open group were lower than endoscopic group. The volume of intraoperative blood loss in the endoscopic group was lower than that in the open group ((87±64) ml vs. (62± 36) ml, t=3.210, P=0.002). Patients′satisfaction of breast reconstruction in the endoscopic group was higher than that in the open group. Conclusions Both endoscopic nipple sparing mastectomy with immediat reconstruction using prosthesis implantation and open surgery are safe in oncology. Endoscopic surgery maybe more suitable alternative in breast reconstruction for early?stage breast cancer patients.