[Objective]To explore echocardiographic features in idiopathic ventricular fibrillation(IVF)patients by multiparametric echocardiographic assessment,in order to evaluate the feasibility of identifying patients at high risk of ven-tricular arrhythmia using echocardiography.[Methods]Patients diagnosed with IVF for the first time in Sun Yat-sen Memo-rial Hospital from January 2019 to December 2023 were included.Age-and sex-matched healthy individuals were includ-ed as control.Multiparametric echocardiographic assessment was performed,involving conventional parameters,myocardi-al mechanical movement parameters,electro-mechanical parameters,and myocardial energetic parameters.Continuous variables were compared using independent samples t-test or Mann-Whitney U test.The receiver operating characteristic(ROC)curves were performed for echocardiographic parameters to assess their diagnostic value for IVF.The intraclass cor-relation coefficient(ICC)was used to assess the inter-observer and intra-observer variability.[Results]Nine patients with IVF and thirty healthy individuals were included in this study.In total,702 left ventricular segments and 117 right ventric-ular free wall segments were analyzed for myocardial function.Left ventricular global longitudinal strain(LV-GLS)was lower in IVF group than in the control group[(18.8±2.7)%vs.(21.2±1.9)%,P=0.004].Left ventricular mechanical dis-persion(LV-MD)was higher in IVF group than in the control group[(44±13)ms vs.(36±9)ms,P=0.022].Global work index(GWI)was lower in IVF group than in the control group[(1991±365)mmHg%vs.(2319±408)mmHg%,P=0.037].Based on the results of the ROC curve test,LV-GLS,LV-MD and GWI had the better diagnostic performance,with the area under the curve(AUC)of 0.748,0.737 and 0.722,the cutoff value of 19.5%,39.5ms and 2049mmHg%,re-spectively.The combination of three indices had the largest AUC value of 0.800.All echocardiographic parameters had ex-cellent intra-observer repeatability and inter-observer reproducibility.The ICC for all parameters was higher than 0.75.[Conclusion]Our results show variable degrees of left ventricular dysfunction are observed in IVF patients by multipara-metric echocardiographic assessment.Decreased LV-GLS,increased LV-MD and decreased GWI are considered high-risk echocardiographic features for malignant arrhythmia.

ObjectiveTo investigate the effects and molecular mechanism of endoplasmic reticalam stress (ERS) on albumin-induced apoptosis in renal proximal tubular cells (HKCs). MethodsWestern blot was performed to detect the relationship of the expression of glucose-regulated protein 78 (GRF78) and CCAAT/enhancer-binding protein-homologous protein (CHOP) with the action time and concentration of haman serum albumin (HSA). Expression levels of CHPO mRNA and protein in HKCs after CHOP siRNA transfection were examined by real-time fluorescence quantitative PCR and Western blot respectively. Annexin-V-FITC and PI doable staining cytometry was used to detect the apoptosis of HKCs induced by HSA and influenced by CHOP siRNA. Results(1)After HKCs were stimulatde by 0, 5, 10, 20 g/L albumin for 24 hours respectively, the expression of GRP78, CHOP and HKCs apoptosis were increased with the albumin concentration (P<0.01). After HKCs were stimulated by 20 g/L albumin for 0, 6, 12, 24, 36 hours respectively, the expression of GRP78 was up-regulated at 6-hour, while CHOP and HKCs apoptosis were increased at 12-hour, and significant differences were found among groups (P<0.01). (2) CHOP siRNA significantly inhibited albumin-induced HKC CHOP mRNA and protein expression, as well as HKC apoptosis (P<0.01). ConclusionsRenal tubular cells exposed to high protein load result in EBS. ERS may subsequently lead to tubular damage by activation of pro-apoptosis factor CHOP.