Objective To summarize the clinical features in idiopathic hypotony maculopathy.Methods A retrospective case series study.Eighteen eyes of 18 patients who were diagnosed with idiopathic hypotony maculopathy were enrolled in the Second Affiliated Hospital of Wenzhou Medical University from August 2012 to December 2017.There were 8 males (8 eyes) and 10 females (10 eyes).All patients underwent examinations including BCVA,optometry,slit lamp microscope,fundus color photography,UBM,B-mode ultrasound,OCT,FFA and axial length (AL).BCVA was recorded with logMAR acuity.The results of affected eyes and contralateral healthy eyes were compared.Paired t test was performed to compare the intraocular pressure (IOP),diopter and AL of the affected eyes and contralateral healthy eyes.Results Among 18 eyes,there were 6 eyes with logMAR BCVA＜ 1.0,10 eyes with logMAR BCVA 1.0-2.0,2 eyes with light perception.The average diopter was +2.32± 1.78 D.The average IOP was 5.18± 1.38 mmHg (1 mmHg=0.133 kPa).The average AL was 20.92± 1.61 mm.The differences of IOP (t=21.6,P＜ 0.000),diopter (t=5.9,P=0.002) and AL (t=9.13,P＜0.000) between the affected eyes and contralateral healthy eyes were significant.The inflammatory reaction in the anterior segment was observed in 13 eyes (72.22％).In the posterior segment,all the eyes were documented with chorioretinal folds,optic disc swelling and retinal phlebectasia were also demonstrated in 14 eyes,while with macular uplift in 7 eyes.In the UBM and gonioscope examination,the angle chamber was open in all patients with ciliary body cyst in 6 eyes,while no ciliary body detachment was detected.All eyes had been examined with B-scan ultrasound and found the increasing thickness of eye ball in all eyes,and nodular changes in the optic papilla in 5 eyes.The chorioretinal folds were further confirmed by OCT with the appearance of the gear shape,much more obviously in the chomid than that in retina.In the FFA,fluorescein leakage was found around the optic disc in 13 eyes at the late stage,while there was no obvious abnormal leakage in the macular or poster part of retina.Conclusions Idiopathic hypotony maculopathy could present with various clinical manifestations.The choroiretinal folds is typical clinical sign.

Objective To summarize the clinical features and visual outcome of posterior scleritis presented with symptoms involving affected optic nerve.Methods Retrospective case series study.Twelve eyes of 12 female patients with posterior scleritis were included in this study.The average age was 35.2± 14.31 years old.The patients got diagnosed with an average of 24.75 ± 22.91 days.Ocular pain was complained in all patients,and blurred vision in 11 patients.The best corrected visual acuity (BCVA),intraocular pressure (IOP),slit lamp microscope examination,B-scan ultrasound,optical coherence tomography (OCT),fundus photography,fundus fluorescein angiography (FFA) and ocular wall thickness measurement were performed in all patients.Nine eyes received visual field examination.All patients received systemic corticosteroid and steroidal eye drops for 3 months.Clinical features and outcome were retrospectively studied.Results Before treatment,the BCVA was from ＜0.1 to ＞0.8.There were 3 eyes with scleral hyperemia,3 eyes with anterior chamber flares,12 eyes with papilledema and different degrees of retinal vein dilatation,3 eyes with star-shaped macular exudates and 2 eyes with macular retinal pigment epithelium detachment.B-scan ultrasound demonstrated that the ocular walls were thickening in all eyes with typical T-sign,and the average thickness was 2.76± 0.68 mm.OCT demonstrated optic disc swelling,and the macular retinal detachment in 2 eyes.In the FFA examination,the fluorescein leakage of the disc was enhanced with time.In the Humphrey test,the value of mean deviation (MD) was 12.56± 5.73 dB and pattern standard deviation (PSD) was 8.15±4.23 dB in 9 eyes before the treatment.After treatment for 3 months,the symptoms were attenuated and the visual acuity was obviously improved with BCVA＞0.1 in all eyes.Scleral hyperemia and anterior chamber flares were only found in 1 eye.The optic disc edema gradually faded away.The ocular wall thickness in the poster part of the eyeball decreased,and the T-sign disappeared in all eyes,the average thickness was 1.53±0.41 mm.Compared with parameters before the treatment,the difference was statistically significant (t=0.003 5,P＜0.05).OCT demonstrated the recovery of the macular retinal detachment.There was no abnormal leakage evidenced in FFA in the optic disc and macular.After treatment,the value of MD and PSD was 5.19±4.82 dB and (4.33 ±3.76) dB,respectively.The difference of MD value between before and after the treatment was significant (t=0.026,P＜0.05).Conclusions Posterior scleritis with an initial symptom of optic nerve was tend to affect middle-aged patients,with clinical manifestations of anterior segment signs in some patients and optic disc swelling with retinal vein dilatation in all patients.B ultrasound examination showed typical T sign.Systemic corticosteroid treatment always obtained remission of the ocular inflammatory activity,and could achieve favorable visual outcome.

[Objective] To analyze the clinical curative effect of 20G and 23G vitrectomy for posterior segment intraocular foreign bodies,to explore the differences of their efficacy and safety.[Methods] This was a retrospective case study.Select 71 patients (71 eyes) who suffered from posterior segment intraocular foreign bodies and underwent different ways of vitrectomy,according to the way of vitrectomy,the patients were enrolled into 20G vitrectomy group (20G group,37 patients,37 eyes) and 23G vitrectomy group (23G group,34 patients,34 eyes).All patients were given wound suture,and patients complicated traumatic cataract should underwent cataract surgery;then were given 20G/23G vitrectomy and extraction of intraction foreign bodies,and (or) be given retinal laser photocoagulation,cryocoagulation,and endotamponade during the procedure.Extraction of intraction foreign bodies and the location of retinal,surgical time,postoperative inflammation and stimulus syndrome,length of hospital stay,the best corrected acuity (BCVA) and other complications after surgery were registered.Minimum follow-up was 6 months.[Results] The rate of extraction of intraction foreign bodies and early retinal reattachment rate were 100％ of the two groups.Comparing the complications after surgery between the two groups,the differences had no statistical significance (all P ＞ 0.05) except the incidence of postoperative ocular hypotenison.The significant difference was found in the comparison of surgical time,average hospitalizcd days,postoperative inflammation score and stimulus syndrome between the two groups,and there were significant differences when the vision distribution before and after surgery in two groups were self-compared (P ＜ 0.05).At lastest follow up,the differences had no statistical significance when comparing the cases of unplanned surgical reoperation,the location of retinal and the vision distribution between the two groups (P ＞ 0.05).[Conclusion] 20G and 23G vitrectomy are both safely and effectively performed in patients with posterior segment intraocular foreign bodies,the efficacy and safety of them are comparable.There is higher incidence of postoperative ocular hypotension in 23G group,and it has an ascendant than 20G group in shorter surgical time and length of hospital stay,lighter postoperative inflamnation and stimulus syndrome.

Objective To investigate the knowledge,attitude and practice (KAP) of blood glucose monitoring and management among non-diabetes specialized nurses in a tertiary hospital of Xiamen,and provide basis for the late intervention.Methods With convenient sampling method,409 non-diabetes specialized nurses from a tertiary hospital of Xiamen were selected as the research object,and they were investigated by self-designed blood sugar monitoring and nursing KAP questionnaire.Results The score of knowledge related to blood sugar monitoring management was (6.52±1.39);the score of attitude related to blood sugar monitoring management was (45.94±4.18);the score of practice related to blood sugar monitoring management was (56.16±6.37);the attitude related to blood glucose monitoring management of non-diabetes specialized nurses was positively correlated with their practice (r=0.119,P=0.016);and nurses' practice was also positively correlated with their knowledge (r=0.190,P=0.027). Among 409 nurses,the percentage of nurses' self-rating mastery degree,general mastery degree,less familiar and unfamiliar in blood sugar management knowledge were 54.8%,37.9%,4.4 and 0.5% %,and only 2.4% of nurses thought they completely master the knowledge. 85.5% of nurses had received training,training organized by nursing department and training in department which includes diabetes link nurses were dominant,and they were 64.1% and 31.5%. The training method was single (72.6%). Theoretical teaching in hospital was still the most popular way,and it accounted for 78.5%. By multiple linear regression analysis,service year was the influencing factors of blood sugar monitoring management. ConclusionsThe knowledge that non-diabetes specialist nurses master is not optimistic,and the level of KAP in blood sugar monitoring still needs to be improved,while full standardization and diversity training are effective methods.

Objective To revise the individual adaptability measure (I-ADAPT-M) developed by Ployhart & Bliese,and analyze the reliability and validity of its application in college freshmen.Methods Revise the I-ADAPT-M,One thousand and seven hundred freshmen were asked to complete the test,one month later,two hundred and seventy-seven freshmen were simple randomly chosen to report their General Life Satisfaction.Then,the data were analysis by SPSS 18.0 and Amos 4.0.Results The exploratory factor analysis showed that,the Chineseversion of the I-ADAPT-M consisted of 23 projects and 4 dimensions (crisis and creativity adaptability,interpersonal adaptability,stress tolerance adaptability,and resistant learning adaptability),which could account for over 45.344％ of total variance.The Cronbacha coefficient of the Chinese-version of the I-ADAPT-M was 0.82.The confirmatory factor analyzed further revealed the structure of four-dimensional scale(x2=708.80,x2/df =3.16,CFI =0.95,NNFI =0.94,RMSEA =0.051,SRMR =0.053).Male had significant high core in crisis and creativity adaptability and resistant learning adaptability than female.Female had significant high core in interpersonal adaptability than male.Colleges-leader had significant high core in 4 dimensions than general students,it suggested that the Chinese-version of the I-ADAPT-M had good discriminant validity.General Life Satisfaction of the subjects had significant positive correlation with crisis and creativity adaptability,stress tolerance adaptability and interpersonal adaptability,had little correlation with resistant learning adaptability.Conclusion The Chinese-version of the I-A-DAPT-M has good reliability and validity to measure the individual adaptability of the freshman.

Objective:To observe the clinical curative effect of integrated traditional Chinese and western medicine in the treat-ment of patients with ocular herpes zoster. Methods:Seventy patients with ocular herpes zoster in our hospital were selected during De-cember 2011 to December 2013. According to random number table,the patients were divided into two groups. The patients in the con-trol group were treated with ganciclovir 5. 0 mg·kg-1 ·h-1 , ivd, qd and ganciclovir ophthalmic gel, qid. The patients in the observa-tion group were treated with Banlangen granules and acupuncture additionally. The clinical efficacy, the time of blistering, pain and crusting and adverse reactions in the two groups were observed and compared. Results:After the treatment, the effective rate of the ob-servation group was 94. 29% , while that of the control group was 77. 14% (P<0. 05). The time of blistering, pain and crusting in the observation group was significantly shorter than that in the control group (P<0. 05). The incidence of adverse reactions of the ob-servation group was 2. 86%, while that of the control group was 11. 43%(P>0. 05). Conclusion:The clinical efficacy of integrated traditional Chinese and western medicine in the treatment of ocular herpes zoster is obviously better than that of western medicine treat-ment alone with fewer adverse reactions and shorter time of blistering, pain and crusting.

OBJECTIVETo develop a spectral-domain optical coherence tomography (SD-OCT) system, for precisely imaging the accommodation of the anterior segment of the human eye.

METHODSDesign the SD-OCT system based on two Michelson interferometer by using the basic principal of weak coherent light interference and modular design of dual channel dual focus.

RESULTSThe system can provide simultaneous cross-sectional imaging of all the surfaces of the anterior segment of the eye including the cornea, anterior chamber, anterior and posterior surfaces of the crystalline lens. Thus, the dynamic change of modification of the curvatures can be calculated in real time.

CONCLUSIONSThe system was successfully tested in imaging accommodation, with high resolution and non contact. The preliminary results demonstrated the feasibility of this novel approach to measure the eye parameters.

Objective To observe the rehabilitation of Six Healing Sounds on MCI of the oldaged persons.Methods All patients were randomly divided into Six Healing Sounds exercise group and control group using random number table.MOCA,and MMSE were adopted for evaluating the efficacy of the subjects before and six months after the practice.Nerve-physiology information workstation was setup to record the changes of cerebral electric powder of both groups before and 6 months after the treatment.The data was further analyzed by intelligent cerebral powder analyzing system.Results ①After six months practice,total score of MOCA (25.18 ± 5.07) and MMSE (26.03 ± 2.63) increased significantly after the treatment in the exercise group,which showed significant difference than the control group [MOCA (22.83 ± 4.95) and MMSE (24.86± 2.98)].MOCA increased significantly in attention (5.04 ± 0.90),delay memory (3.02 ± 0.89),verbal(2.30±0.57) sub-item scores after the treatment in the exercise group,showed significant differences than the control group [attention (4.28 ± 1.08),delay memory (2.33 ± 0.95),verbal (2.13 ± 0.51)] ; MMSE also demonstrated a significant increase a in split-second memory(2.35 ±0.43),delay memory(2.42±0.48),verbal (8.49± 0.54) sub-item scores after the treatment in the exercise group,showed significant differences than the control group [split-second memory (2.15 ± 0.34),delay memory (2.14± 0.83),verba (8.13 ± 0.70)].②Frequency changes:the change of δ (0.0848± 0.0373) μ v 2 was decreased than before in the exercise group after 6 months; the change of α1 (0.3753± 0.1470) μ v2was increased than before in the exercise group after 6 months; the change ofδ and θ (0.2266±0.0953)μ v2 had significant difference after the treatment between the two group [control group:δ (0.1095 ± 0.0520) μ v2 θ (0.2589 ± 0.0862) μ v 2and α1 (0.3160 ±0.1540)μ v 2].③Space changes:in the ratio of (δ + θ) / (α1+α2+β1+β2):F4(0.5115±0.4262)、O 1 (0.3365 ± 0.2436)、O2 (0.3536± 0.2671)、T4 (0.4581 ± 0.3798)、T5 (0.3198± 0.3608)、Fp (10.8233 ±0.6857) 、Fp2 (0.7710± 0.6352)、C3 (0.3702 ± 0.3910)、C4 (0.3896 ± 0.3523)、P4 (0.3523 ± 0.2333)、T6 (0.2972± 0.4276)were decreased than before in the exercise group after 6 months; C3、C4、P4、T4、T5 、T6、Fpl had significant difference after the treatment between the two group[control group:C3 (0.5706±0.4944)、C4 (0.5780 ± 0.4996)、P4 (0.4835 ± 0.3641)、T4 (0.6294 ± 0.4186)、T5 (0.4882 ± 0.4000)、T6 (0.4879±0.4131)、Fp1 (1.0125±0.7476)].Conclusion Six Healing Sounds had rehabilitation effects on MCI of the old-aged persons.

Objective To develop a spectral-domain optical coherence tomography(SD-OCT)system , for precisely imaging the accommodation of the anterior segment of the human eye. Methods Design the SD-OCT system based on two Michelson interferometer by using the basic principal of weak coherent light interference and modular design of dual channel dual focus. Results The system can provide simultaneous cross-sectional imaging of al the surfaces of the anterior segment of the eye including the cornea, anterior chamber, anterior and posterior surfaces of the crystal ine lens. Thus, the dynamic change of modification of the curvatures can be calculated in real time. Conclusions The system was successful y tested in imaging accommodation, with high resolution and non contact. The preliminary results demonstrated the feasibility of this novel approach to measure the eye parameters.

PURPOSE: Circulating free DNA (cfDNA) in plasma is promising to be a surrogate for tumor tissue DNA. However, not all epidermal growth factor receptor (EGFR) mutations in tumor tissue DNA has been detected in matched cfDNA, at least partly due to inefficient cfDNA extraction method. The purpose of this study was to establish an efficient plasma cfDNA extraction protocol. MATERIALS AND METHODS: The yield of plasma cfDNA extracted by our modified phenol-chloroform (MPC) method from non-small-cell lung cancer (NSCLC) patients was compared with that by QIAamp MinElute Virus Spin kit (Qiagen kit) as control, using the Wilcoxon rank-sum test. TaqMan quantitative polymerase chain reaction (qPCR) assays were used to quantify the plasma cfDNA extracted. Both Mutant-enriched PCR (ME-PCR) coupled sequencing and DxS EGFR mutation test kit were used to evaluate the impact of extraction method on EGFR mutation analysis. RESULTS: MPC method extracted more plasma cfDNA than Qiagen kit method (p=0.011). The proportion of longer fragment (> or =202 bp) in cfDNA extracted by MPC method was significantly higher than by Qiagen kit method (p=0.002). In the sequencing maps of ME-PCR products, a higher mutant peak was observed on plasma cfDNA extracted by MPC method than by Qiagen kit method. In DxS EGFR mutation test kit results, plasma cfDNA extracted by MPC method contained more tumor-origin DNA than by Qiagen kit method. CONCLUSION: An improved plasma cfDNA extraction method of MPC is provided, which will be beneficial for EGFR mutation analysis for patients with NSCLC.
Base Sequence ; Carcinoma, Non-Small-Cell Lung/*genetics ; Chloroform ; DNA Mutational Analysis/*methods ; DNA, Neoplasm/*blood/*isolation & purification ; Genetic Testing/methods ; Humans ; Lung Neoplasms/*genetics ; Molecular Sequence Data ; Phenol ; Polymerase Chain Reaction/methods ; Receptor, Epidermal Growth Factor/*genetics