AIM: To explore the clinical effect of Tongzhihuoluo Granules combined with compound anisodine on the acquired ophthalmoplegia in the middle-aged and elderly.

METHODS:We selected 81 patients(81 eyes)with acquired ophthalmoplegia in the middle-aged and elderly who were diagnosed and treated in our hospital from January 2015 to October 2017. All patients were divided into observation group(n=40)and control group(n=41)by treatment methods. The control group was given subcutaneous injection of compound anisodine 2mL near the superficial temporal artery of the affected side. The observation group was combined with Tongzhihuoluo Granules. The clinical efficacy and diplopia distance were compared between the two groups. The pulsatility index and blood flow velocity of the basilar artery before and after treatment were compared between the two groups.

RESULTS: The effective rate of ophthalmoplegia in the observation group was 92%(37/40), which was significantly higher than that in the control group(73%, 30/41; P<0.05). The incidence of cure time ≤ 2wk in the observation group was significantly higher than that in the control group(P<0.05). The recombination distances after treatment in both groups were significantly shortened(P<0.01), and the diplopia distance in the observation group was significantly shorter than that in the control group(P<0.01). The pulsation index of both groups were significantly lower after treatment(P<0.01), and the blood flow velocity was significantly increased(P<0.01), and those of the observation group changed more(P<0.01).

CONCLUSION: The clinical therapeutic effect of Tongzhihuoluo Granules combined with compound anisodine on the acquired ophthalmoplegia in middle-aged and elderly patients is significantly better than that of compound anisodine, which can effectively improve the blood flow of vertebral-basal artery and has high clinical efficacy.

OBJECTIVETo analyze the trend of overweight and obesity in students in China during 1985 - 2010.

METHODSThe data were from Chinese National Survey on Students Constitution and Health in 1985, 1995, 2000, 2005 and 2010, and the sample sizes were 204 977, 216 786, 234 421, 215 319, respectively. BMI was calculated with height and weight. The prevalence of overweight and obesity as well as the rates of increase were described based on the BMI percentile criteria of Working Group on Obesity in China (WGOC).

RESULTSIn 2010, the prevalence of overweight and obesity in four groups students (urban boys, urban girls, rural boys, and rural girls aged 7 - 18 years) were 23.23% (12 503/53 830), 13.76% (7 414/53 857), 12.72% (6 839/53 744), 8.56% (4 612/53 888), respectively, which were higher in boys and urban, and reached the peak 17.14% (18 463/107 741) in students aged 7 - 12 years. The prevalences of obesity were 0.44% (8/1800) - 21.67% (390/1800) among children aged 7 - 18 years in different provinces. The prevalence of overweight and obesity during 1985 - 1995, 1995 - 2000, 2000 - 2005 and 2005 - 2010 increased 0.10%, 0.30%, 0.23%, 0.23%, respectively, and the increase rate was higher in urban than in rural during 1985 - 2005. During 1985 - 1995, the increase rate of obesity in four groups students were 0.20%, 0.13%, 0.05% and 0.03%, respectively. During 2005 - 2010, the increase rates of obesity in four groups of students were 0.30%, 0.10%, 0.34%, 0.17%, respectively, and the increase rate was higher in rural than in urban.

CONCLUSIONThe prevalence of obesity among 7 - 18 years-old children increased rapidly during the 1985-2000, and the increase rate of obesity in rural exceeded that in urban during 2005 - 2010.

Adolescent ; Body Mass Index ; Child ; China ; epidemiology ; Female ; Health Surveys ; Humans ; Male ; Obesity ; epidemiology ; Overweight ; epidemiology ; Rural Population ; Students ; Urban Population

BACKGROUNDScreening libraries against a molecular target in vitro are idealized models that cannot reflect the real state in vivo where biomolecules coexist and interact. C-terminal amide tripeptides labelled with Technetium-99m can provide a unique noninvasive approach to trace a large number of compounds in vivo.

METHODSThe C-terminal amide tripeptide libraries were synthesized on Rink Amide-MBHA resin using iterative and pooling protocol. Technetium (V) oxo core [TcO(3+)] was bound to each tripeptide via 4 deprotonated nitrogen atoms to form a library of 8000 (99m)Tc tripeptoid complexes. The radiocombinatorial screening (RCS) in vivo was carried out on SD rats and A549 tumour bearing mice.

RESULTSSignals of tissue distribution and metabolism of libraries were recorded by counting or imaging and tissue targeting leads identified by both random and directed RCS. Among them, (99m)Tc RPA, (99m)Tc VIG and (99m)Tc RES had specific tissue targeting in kidney, liver and tumour respectively. The percent injected dose per gram tissue of (99m)Tc labelled leads in their target tissue was highly structure dependent. Because the nontarget tissue binding and the metabolism of (99m)Tc tripeptoid sublibraries were simultaneously monitored successfully by RCS, the interference of background activity was limited to the lowest level. Optimization of renal function agent from the labelled libraries was carried out by directed screening. (99m)Tc DSG was finally identified the most promising agent for renal function studies.

CONCLUSIONSRCS in vivo is a powerful tool for the discovery of tissue targeting drugs. The potential screening bias is probably the major limitation of labelled libraries.

Animals ; Combinatorial Chemistry Techniques ; Drug Design ; Female ; Isotope Labeling ; Kidney Function Tests ; Liver ; diagnostic imaging ; Mice ; Mice, SCID ; Neoplasms, Experimental ; diagnostic imaging ; Peptide Library ; Radionuclide Imaging ; Radiopharmaceuticals ; chemical synthesis ; Rats ; Rats, Sprague-Dawley ; Technetium ; Tissue Distribution

OBJECTIVETo investigate the relationship between the blood flow of the exceedingly expanded skin and the viability of the flap.

METHODSThe piglets were set up as the animal model. They were divided into 7 groups according to different injection volume of the tissue expander, i. e. injection to 1/2 content, 3/4 content, full content, 50% over content, 100% over content, sham-operation control and normal control. At different stages of expansion, the blood flow and the amplitude of the expanded skin were examined by laser Doppler. The survival length of the expanded flap was measured. Furthermore, statistical analysis was performed.

RESULTSAs the expansion volume increasing, the blood flow and the amplitude of the expanded skin increased, the survival length of the expanded flap increased also. In the meantime, the amplitude instability and vessel permeability increased. When the injection volume exceeded to 100%, the blood flow and the survival length of the expanded flap decreased.

CONCLUSIONSIn soft tissue and skin expansion, increasing injection volume could increase the survival length of the expanded flap, while over-expansion to 100% content may cause blood flow disturbance.

Animals ; Graft Survival ; Models, Animal ; Skin ; blood supply ; Skin Transplantation ; Surgical Flaps ; blood supply ; Swine ; Swine, Miniature ; Tissue Expansion Devices