Infiltration and activation of peripheral immune cells are critical in the progression of multiple sclerosis and its experimental animal model, experimental autoimmune encephalomyelitis (EAE). This study investigates the role of high mobility group box 1 (HMGB1) in oligodendrocyte precursor cells (OPCs) in modulating pathogenic T cells infiltrating the central nervous system through the blood-brain barrier (BBB) by using OPC-specific HMGB1 knockout (KO) mice. We found that HMGB1 released from OPCs promotes BBB disruption, subsequently allowing increased immune cell infiltration. The migration of CD4+ T cells isolated from EAE-induced mice was enhanced when co-cultured with OPCs compared to oligodendrocytes (OLs). OPC-specific HMGB1 KO mice exhibited lower BBB permeability and reduced immune cell infiltration into the CNS, leading to less damage to the myelin sheath and mitigated EAE progression. CD4+ T cell migration was also reduced when co-cultured with HMGB1 knock-out OPCs. Our findings reveal that HMGB1 secretion from OPCs is crucial for regulating immune cell infiltration and provides insights into the immunomodulatory function of OPCs in autoimmune diseases.
Animals ; Encephalomyelitis, Autoimmune, Experimental/metabolism* ; HMGB1 Protein/deficiency* ; Mice, Knockout ; Oligodendrocyte Precursor Cells/immunology* ; Mice, Inbred C57BL ; CD4-Positive T-Lymphocytes/immunology* ; Cell Movement ; Blood-Brain Barrier/immunology* ; Mice ; Myelin Sheath/pathology* ; Disease Models, Animal ; Coculture Techniques ; Oligodendroglia/metabolism* ; Female ; Cells, Cultured

PURPOSE: This study tries to systematically understand factors that explain levels of happiness among pregnant women in the Ecological systems theory. METHODS: A descriptive, cross-sectional study was conducted with 169 pregnant women in Korea. Collected data from self-report questionnaires were analyzed by hierarchical regression analysis using the SPSS statistics 23 program. RESULTS: A total of 5 models were examined according to individual, microsystem, mesosystem, exosystem, and macrosystem in the Ecological systems theory. In the first model including individual factors, extraversion, neuroticism, and physical and psychological change constitute significant factors explaining happiness. In the second model with microsystem factors and in the third one with mesosystem factors, marital intimacy appears to be a significant factor. In the fourth model including exosystem factors, community service is a significant factor. In the final model with social atmosphere, personality (β=.15 for extraversion; β=−.30 for neuroticism), physical and psychological change (β=−.15), marital intimacy (β=.35), and community service (β= .18) turn out to be significant. These factors explain 59% of the variance of happiness in the pregnant women in Korea. CONCLUSION: Considering the fact that pregnant women's happiness is explained by microsystem and exosystem factors as well as individual factors, developing intervention programs that can promote influencing factors such as marital intimacy and community service is necessary to improve levels of happiness among pregnant women in Korea.
Atmosphere ; Community Health Services ; Cross-Sectional Studies ; Ecology ; Ecosystem ; Extraversion (Psychology) ; Family Characteristics ; Female ; Happiness ; Humans ; Korea ; Pregnancy ; Pregnant Women ; Social Welfare