Objective:To compare the dosimetric difference between 3D-printed multi-channel applicator and conventional vaginal single-channel applicator for brachytherapy, aiming to provide guidance for patients receiving brachytherapy after cervical cancer surgery.Methods:From January 2019 to November 2020, 25 cervical cancer patients complicated with VAIN Ⅲ receiving 192Ir high-dose-rate brachytherapy after cervical cancer surgery were selected. Each patient was located by CT scanning with 3D-printed multi-channel applicator and conventional vaginal single-channel applicator, and corresponding plan and evaluation were carried out. The dose volume histogram (DVH) was obtained by inverse dose optimization algorithm. The dosimetric differences of high-risk clinical target volume (HRCTV), bladder and rectum during brachytherapy were compared with those of source applicators. The optimal treatment plan was selected. Results:D 90%, D 100%, V 100% and V 150% of the plans designed by 3D-printed individual multi-channel applicator had no significant differences compared with those designed by conventional single-channel applicator (all P>0.05). The bladder and rectal D 2cm 3 designed by 3D-printed multi-channel applicator were significantly lower than those using conventional single-channel applicator, and the differences were statistically significant (both P<0.05). Conclusion:The multi-channel individual applicator target made by 3D-printing technology has good conformal property, properly protects the bladder and rectum and possesses treatment advantages over conventional single-channel applicator.

ObjectiveTo investigate the effects of gallic acid （GA） on human colon cancer HCT-116 and Caco-2 cell activities， intracellular Janus kinase （JAK）/signal transducer and activator of transcription factor （STAT） signaling pathway， and the expression of anti-apoptotic protein B-cell lymphoma-2 （Bcl-2） and pro-apoptotic protein B-cell lymphoma-2-associated X protein （Bax）， so as to explore its underlying molecular mechanism. MethodFollowing the classification of cells into GA group， blank group， and 5-fluorouracil （5-FU， 0.05 g·L^-1） group， the HCT-116 and Caco-2 cells were treated with GA （0.02， 0.05， 0.1， 0.15， 0.2 g·L^-1） for 12， 24， 48， and 72 h， respectively， and the cell proliferation inhibition rats were determined by cell counting kit-8 （CCK-8） assay to select the GA concentration that effectively inhibited proliferation. The colony formation ability was detected by crystal violet staining and the migration of cells by scratch test. The level of reactive oxygen species （ROS） was measured using a fluorescent probe （DCFH-DA）. The expression of interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） in cell supernatant were determined using the enzyme-linked immunosorbent assay （ELISA） kits. The expression levels of JAK2， phosphorylated （p）-JAK2， STAT3， p-STAT3， Bcl-2， and Bax were assayed by Western blot. ResultCCK-8 assay showed that after 12， 24， 48， and 72 h of treatment， GA （0.02， 0.05， 0.1， 0.15， 0.2 g·L^-1） inhibited the proliferation of HCT-116 and Caco-2 cells in a dose- and time-dependent manner， and the inhibition rates were higher than those in the blank control group. Compared with the 5-FU group， GA （0.2 g·L^-1） enhanced the inhibition of cell proliferation in a time-dependent manner. Compared with the blank control group， GA （0.1， 0.15， and 0.2 g·L^-1） significantly decreased the number of cell colonies （P<0.01）， increased the inhibition rate of cell colony formation （P<0.01）， diminished the scratch healing rate （P<0.05， P<0.01）， elevated the fluorescence intensity of intracellular ROS （P<0.01）， and down-regulated the expression of IL-6 and TNF-α in the supernatant （P<0.01） in a dose-dependent manner. Compared with the 5-FU group， GA （0.2 g·L^-1） decreased the scratch healing rate （P<0.01）， enhanced the fluorescence intensity of intracellular ROS （P<0.01）， and down-regulated the levels of IL-6 and TNF-α in cell supernatant （P<0.01）. According to Western blot analysis， compared with the blank control group， GA （0.1， 0.15， 0.2 g·L^-1） obviously lowered the expression of p-JAK2， p-STAT3， Bcl-2， p-JAK2/JAK2， p-STAT3/STAT3， and Bcl-2/Bax （P<0.01） and raised Bax protein expression （P<0.05， P<0.01） in a dose-dependent manner. Compared with the 5-FU group， GA （0.2 g·L^-1） down-regulated the expression of p-JAK2， p-STAT3， Bcl-2， p-JAK2/JAK2， p-STAT3/STAT3， and Bcl-2/Bax （P<0.05， P<0.01） and up-regulated the expression of Bax protein （P<0.05， P<0.01）. ConclusionGA significantly inhibits the proliferation of HCT-116 and Caco-2 cells， which may be related to the increased accumulation of intracellular ROS， down-regulation of inflammatory factors IL-6 and TNF-α， p-JAK2 and p-STAT3 protein expression in JAK/STAT signaling pathway， and Bcl-2， and up-regulation of Bax.

Objective:To observe the relationship between peripheral blood T cell subsets and clinical characteristics and prognosis of Uygur women with advanced cervical squamous cell carcinoma in Xinjiang.Methods:A total of 185 patients pathologically diagnosed with stage Ⅱ B-IVA cervical squamous cell carcinoma admitted to Cancer Hospital Affiliated to Xinjiang Medical University from January 2015 to December 2018 were selected. The relationship between T cell subsets in peripheral venous blood and clinical characteristics and prognosis was analyzed. Results:CD 4+ T cells, CD 8+ T cells and CD 4+/CD 8+ T cell ratio were significantly correlated with clinical stage, tumor diameter and body mass index (BMI)(all P<0.05). The later Federation International Association of Gynecology and Obstetrics (FIGO) tumor stage, the larger the tumor diameter, the higher the BMI, and the higher the CD 8+ T cells and the lower the CD 4+ T cell and CD 4+/CD 8+ T cell ratio. The count of CD 4+ T cells was decreased in patients with lymph node metastasis. Cox’s univariate analysis showed that FIGO stage, age, lymph node metastasis, tumor diameter, BMI, CD 4+ T cells, CD 8+ T cells, CD 4+/CD 8+ T cell ratio and treatment methods were the important factors affecting the overall survival (OS). Multivariate analysis showed that BMI, treatment method, CD 4+ T cells and tumor diameter were the independent prognostic factors affecting OS (all P<0.05). Conclusions:The level of T cell subsets in peripheral blood of Uygur cervical cancer patients is out of balance. CD 4+ T cells, CD 8+ T cells and CD 4+/CD 8+ T cell ratio are associated with FIGO stage, tumor diameter and BMI, and CD 4+ T cells are correlated with lymph node metastasis. BMI, treatment method, tumor diameter and CD 4+ T cell are the independent prognostic factors affecting the OS of patients with cervical cancer.

Objective: To explore the poor prognostic factors of patients with cervical stump carcinoma, aiming to provide certain reference for the clinical diagnosis and treatment. Methods: Clinical data of 48 patients with cervical stump carcinoma admitted to the Affiliated Tumor Hospital of Xinjiang Medical University from January 1, 2005 to December 1, 2016 were retrospectively analyzed. A total of 19 patients (40%) withⅠ_A-Ⅱ_A stage cervical stump carcinoma were treated with surgery+ adjuvant therapy and 29 patients (60%) in Ⅱ_B-Ⅳ stage received radiotherapy combined with chemotherapy. The median age of onset was 51 years old. Uterine fibroids were the main cause of subtotal hysterectomy. The average time interval from subtotal hysterectomy to definite diagnosis was 10.76 years. Results: The 1-, 3-, 5-year survival rate was 98%, 83% and 74%, respectively. Univariate analysis demonstrated the time interval from subtotal hysterectomy (P=0.016), tumor diameter (P=0.016), clinical stage (P=0.036), histological grade (P=0.009), lymph node metastasis (P=0.044), parametrial invasion (P=0.046), myelosuppression (P=0.013) and radical surgery (P=0.019) were the poor prognostic factors of cervical stump carcinoma. Conclusions Poor prognosis of patients with cervical stump carcinoma is correlated with tumor diameter, clinical stage, histological grade, lymph node metastasis, parametrial invasion and myelosuppression. Histological grade is an independent risk factor.

Objective To investigate the clinical features and treatment of vaginal intraepithelial neoplasia (VAIN).Methods Clinical data of 50 patients with VAIN,aged 33-76 years,were retrospectively analyzed.Among them,16 patients were diagnosed with grade Ⅰ VAIN,5 with grade Ⅱ VAIN and 29 with grade Ⅲ VAIN.Results A majority of VAIN patients presented with no clinical symptoms,whereas few cases were manifested with increased vaginal secretion.The lesions were located in the vaginal fornix or the upper 1/3 of the vagina in 82％ of patients,and in the middle and lower 1/3 of the vagina in the remaining 18％ cases.Patients with VAIN Ⅰ were observed during follow-up,VAIN Ⅱ patients were given with local administration of medication,and VAIN Ⅲ patients received three-dimensional high-dose rate after loading intracavitary radiotherapy.Human papilloma virus (HPV) infection was positively correlated with VAIN grade (P=0.028).Conclusions The VAIN grade is positively correlated with high-risk HPV infection.Afterloading intracavitary radiotherapy is an efficacious therapeutic approach for VAIN Ⅲ patients,which yields slight adverse events and high cure rate.

This study assesses the causes and prevention measures of rabies through epidemiological investigation and analysis. A field epidemiological survey was conducted to investigate a case of rabies by fox bite. The onset of symptoms began 50 days after the bite. The patient did not receive standard treatment, rabies vaccination, or rabies immunoglobulin injection. The fox was killed on the spot. Saliva and pre-death blood samples were collected at different periods, and only blood RT-PCR tests yielded positive results. Wild fox bite is a major risk factor of rabies infection in Xinjiang Province, China.
Adult ; Animals ; Brain ; virology ; China ; epidemiology ; Fatal Outcome ; Foxes ; virology ; Humans ; Male ; Rabies ; epidemiology ; transmission ; Zoonoses

OBJECTIVE: Using a pulsating coronary artery phantom at high heart rate settings, we investigated the efficacy of a motion correction algorithm (MCA) to improve the image quality in dual-energy spectral coronary CT angiography (CCTA). MATERIALS AND METHODS: Coronary flow phantoms were scanned at heart rates of 60–100 beats/min at 10-beats/min increments, using dual-energy spectral CT mode. Virtual monochromatic images were reconstructed from 50 to 90 keV at 10-keV increments. Two blinded observers assessed image quality using a 4-point Likert Scale (1 = non-diagnostic, 4 = excellent) and the fraction of interpretable segments using MCA versus conventional algorithm (CA). Comparison of variables was performed with the Wilcoxon rank sum test and McNemar test. RESULTS: At heart rates of 70, 80, 90, and 100 beats/min, images with MCA were rated as higher image scores compared to those with CA on monochromatic levels of 50, 60, and 70 keV (each p < 0.05). Meanwhile, at a heart rate of 90 beats/min, image interpretability was improved by MCA at a monochromatic level of 60 keV (p < 0.05) and 70 keV (p < 0.05). At a heart rate of 100 beats/min, image interpretability was improved by MCA at monochromatic levels of 50 keV (from 69.4% to 86.1%, p < 0.05), 60 keV (from 55.6% to 83.3%, p < 0.05) and 70 keV (from 33.3% to 69.3%, p < 0.05). CONCLUSION: Low-keV monochromatic images combined with MCA improves image quality and image interpretability in CCTAs at high heart rates.
Angiography* ; Coronary Vessels* ; Heart Rate* ; Heart* ; Tomography, X-Ray Computed

Objective Systematic pelvic lymph node ( SPLN) +abdominal paraaortic lymph node ( APLN) dissection re-mains controversial in the treatment of endometrial carcinoma , especially in the early stage of the tumor .This study aims to investigate the value and safety of APLN dissection in the treatment of early-stage endometrial carcinoma . Methods We retrospectively ana-lyzed the clinical data about 109 cases of early-stage endometrial adenocarcinoma , 56 treated by SPLN dissection ( group A ) and the other 53 by SPLN+APLN dissection ( group B ) .We compared the postoperative complications , recurrence and metastasis , and progno-sis-related factors between the two groups of patients . Results No statistically significant difference was found in the incidence rate of postoperative complications between groups A and B ( 19.64% vs26.41%, P>0.05).Recurrence and metastasis were found in 12 of the 109 patients, 10 in group A and 2 in group B (17.86%vs 3.77%, P=0.019).Multivariate logistic regression analysis showed that the independent factors of recurrence and metastasis includ -ed the differentiation degree (OR=7.385, 95%CI:1.877-29.062), pathologic stage (OR=5.444, 95%CI:1.673-17.720), range of lymph mode dissection (OR=19.171, 95%CI:2.242 -163.946), and range of lesion focus (OR=12.524, 95%CI:1.186-132.280), with the range of lymph mode dissection as the greatest influencing factor on prognosis . Conclusion SPLN+APLN dissection can reduce the recurrence and metastasis and improve the prognosis of early -stage endometrial adenocarcinoma , and therefore is safe and feasible for the treatment of the tumor .

OBJECTIVE: To determine the vaginal flora distribution in cervical cancer patients and the common pathogenic bacteria as well as drug resistance, and to explore the correlation of vaginal bacterial infection and high-risk human papillomavirus (HR-HPV) infection with cervical cancer.
 METHODS: A total of 216 patients with cervical cancer served as an experimental group, and 53 patients with chronic cervicitis served as a control group. The patients' vaginal fluid in two groups was collected before the treatment for regular bacterial culture and HPV testing. The distribution and drug resistance of bacteria in two groups of vaginal secretion were observed, and the correlation of the bacterial infection and HPV infection with the cervical cancer was analyzed.
 RESULTS: The gram-negative and gram-positive bacteria accounted for 74.38% and 21.49% in the experimental group, respectively. They were mainly resistant to ampicillin and piperacillin or penicillin and erythromycin. The gram-negative and gram-positive bacteria accounted for 42.31% and 23.08% in the control group, respectively. They were mainly resistant to ampicillin and piperacillin or penicillin. HPV-positive rates in the experiment group and the control group were 60.65% and 41.51%, respectively. There were 70 patients (32.41%) and 12 patients (22.64%) with both bacterial infection and HPV-positive infection in the experiment group and the control group, respectively. However, there was no statistical difference between the 2 groups (P>0.05). 
 CONCLUSION Escherichia coli are the main pathogen in cervical cancer and they are highly resistant to antibiotics. Bacterial infection in genital tract is not an efficient cofactor for HPV to cause the cervical cancer.
Drug Resistance, Bacterial ; Female ; Gram-Negative Bacterial Infections ; Gram-Positive Bacteria ; Gram-Positive Bacterial Infections ; Humans ; Reproductive Tract Infections ; Uterine Cervical Neoplasms

BACKGROUNDThe incidence of congenital coronary artery anomalies (CCAAs) is different between ethnic groups, but there is no report about Uyghur CCAAs because of the limitation of inspection methods. This study determined the prevalence of Uyghur CCAAs and analysis the difference of CCAAs between Uyghur and Han ethnic groups by the method of multi-slice computed tomography coronary angiography (MSCTCA).

METHODSSeven thousand four hundred and sixty-nine MSCTCA were analyzed for the CCAAs retroactively, 1934 were Uyghur patients while 4746 were Han patients. All the coronary artery images dates obtained by MSCTCA were evaluated for the CCAAs by two doctors.

RESULTSNineteen kinds of CCAAs were found: (1) The overall incidence of CCAAs was 2.72% (203/7469) among all patients, 2.34% (111/4746) among Han patients whereas a significant higher 3.93% (76/1934) among Uyghur patients (χ2 = 12.780,P < 0.05); (2) the incidence of CCAAs among male patients was 2.48% (76/3069) in Han while 4.33% (56/1293) in Uyghur (χ2 = 10.663, P < 0.05); (3) the incidence of CCAAs on the left side was 1.07% (51/4746) among Han patients while 2.17% (42/934) among Uyghur patients (χ2 = 12.047, P < 0.05); (4) among these 19 kinds of CCAAs, there were significant differences of the incidence of the following kinds of CCAAs between Uyghur and Han: Left coronary artery (LCA) high location (χ2 = 8.320, P = 0.004), right coronary artery (RCA) originate from left coronary sinus (χ2 = 5.450, P = 0.020), and RCA originate from left Coronary sinus + LCA high location (P = 0.024).

CONCLUSIONSThere exists some difference in CCAAs between Uyghur and Han ethnic groups. The CCAAs incidence of Uyghur is higher than that of Han, especially in male patients and on the left side; among all kinds of CCAAs, the incidence of LCA high location, RCA originate from left coronary sinus, RCA originate from left coronary sinus + LCA high locations of Uyghur is higher than Han.

Aged ; China ; Coronary Artery Disease ; diagnosis ; diagnostic imaging ; ethnology ; Coronary Vessel Anomalies ; diagnosis ; diagnostic imaging ; ethnology ; Ethnic Groups ; statistics & numerical data ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Tomography, X-Ray Computed