Objective:To describe the changes of post-traumatic growth and rumination in coronary heart disease patients at different time points after PCI, and to analyze the correlation between them in order to provide evidence for personalized intervention.Methods:A cross-sectional study was conducted, a total of 234 patients with coronary heart disease who underwent PCI for the first time in the Department of Cardiology, the First Affiliated Hospital of China Medical University from December 2021 to October 2022 were studied by convenience sampling. The general data questionnaire, the Chinese version of the Event-Related Rumination Inventory and the simplified Chinese version of the Posttraumatic Growth Inventory were used to survey before discharge (T 0), first month after surgery (T 1), and three months after surgery (T 2). Results:The total scores of post-traumatic growth at the time point of T 0, T 1 and T 2 were 53.08 ± 13.47, 54.77 ± 11.20 and 59.32 ± 7.95, the difference was statistically significant ( F = 78.45, P<0.01). The scores of instrusive rumination at the time point of T 0, T 1 and T 2 were 14.92 ± 6.07, 15.78 ± 4.64 and 12.31 ± 3.38, the difference was statistically significant ( F = 46.58, P<0.01). The scores of deliberate rumination were 14.63 ± 4.74, 15.52 ± 3.93 and 17.86 ± 3.70, the difference was statistically significant ( F = 76.20, P<0.01). Instrusive rumination at T 0, T 1 and deliberate rumination at each point were positively correlated with the total score of post-traumatic growth ( r values were 0.229 to 0.414, all P<0.01). Instrusive rumination at T 2 was negatively correlated with posttraumatic growth scores ( r = -0.351, P<0.01). The deliberate rumination at T 2 time point was positively correlated with the total score of post-traumatic growth ( r = 0.403, P<0.01). Conclusions:The level of posttraumatic growth in patients with coronary heart disease after PCI needs to be improved, and targeted intervention should be planned early to change patients′pessimistic cognition caused by the disease, promote the development of positive thinking, then promote their posttraumatic growth.

Objective: To develop the chimeric antibodies against neuraminidase (NA) of H7N9 and to identify their biological activity and function. Methods: The genes of variable regions of the light chain (VL) and heavy chain (VH) obtained by mouse hybridoma technology were cloned respectively into the expression VH and VL vectors bearing human-derived Cγ1, and Cκ1 and co-transfected into 293T cells. The chimeric antibodies were purified and their functions were investigated. Results: Two chimeric antibodies, 1E2 and 3E3 against neuraminidase (NA) of H7N9 were obtained. Both antibodies recognized similar antigenic epitopes. MAb 1E2 and 3E3 could prevent the infectivity with H7N9 and H11N9 virus and reduce their size of viral plaque. Conclusions The chimeric antibodies specific for N9 could prevent the infection of N9 subtype influenza virus as well as the NAI-resistant mutants and could be a potential immunotherapy approach for H7N9 treatment.

Objective: To isolate the cross-reactive antibodies against hemagglutinin of influenza virus and identify its biological function. Methods: The antibodies gene reservoir of cross-reactive and H5N1 pseudotype particles neutralizing B cell circulating in peripheral blood of a human H5N1 case was recovered by in vitro B cell culture, screening, RT-PCR and expression vector cloning techniques. The Ab gene pairing was screened by transient transfection of human kidney 293T cells and detected using ELISA and neutralization test. The heterosubtypic antibodies were prepared and characterized. Results: We discovered the VH1-2-based heterosubtypic antibodies from two B cell lineages could neutralize GX-H5N1 pseudotype particles and have broader binding with Group 1 (including H1, H5, H6 and H9) and H7 subtype. Conclusions Cross-reactive antibodies can be induced by H5N1 infection.