Purpose To investigate whether ketogenic diet(KD)can promote cognition by regulating brain metabolism and neuroinflammation in Alzheimer's disease model mice.Materials and Methods Twenty male APP/PS1 mice were randomly assigned to either a KD group(APP/PS1+KD)or a regular diet group(APP/PS1),with 10 mice in each group.Additionally,10 wild-type C57BL/6 male mice served as the control group.The APP/PS1+KD group was fed with a ketogenic feed,the APP/PS1 group received a regular diet,and the control group was maintained on standard chow for a duration of 4 months.Blood ketone levels of mice were monitored after 4 weeks and 4 months of continuous feeding.Cognitive function was assessed via the morris water maze.18F-FDG and 18F-DPA-714 micro PET/CT were performed to evaluate the effects of KD on glucose metabolism and neuroinflammation across various brain regions in the Alzheimer's disease mice.Following PET/CT imaging,brain tissue samples were collected,and the hippocampal Cal region was selected for paraffin sectioning to detect the expression of glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1 through immunofluorescence analysis.Results In the Morris water maze in the fourth month,compared with the control group,the APP/PS1 group had a significantly longer escape latency on days 3-4(P＜0.05 or P＜0.01).Compared with the control group,the APP/PS1 group showed a significant decrease in relative 18F-FDG uptake in brain regions such as the striatum,hippocampus,dorsal thalamus,central gray matter,superior colliculus,olfactory bulb,and midbrain(P＜0.01,P＜0.05).Compared with the APP/PS1 group,the APP/PS1+KD group showed a significant increase in relative 18F-FDG uptake in the hippocampus and dorsal thalamus(P＜0.01).Compared with the control group,the APP/PS1 group showed a significant increase in relative uptake of 18F-DPA-714 in brain regions such as the striatum,hippocampus and hypothalamus(P＜0.05 or P＜0.001).Compared with the APP/PS1 group,the APP/PS1+KD group decreased the relative uptake of 18F-DPA-714 in the hippocampus(P＜0.01).Compared with the control group and APP/PS1+KD group,the fluorescence intensity of glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1 protein in the brain(hippocampus)of APP/PS1 group mice was significantly increased(both P＜0.01).Conclusion KD has the potential to ameliorate cognitive and behavioral deficits in APP/PS1 mice by enhancing brain metabolism and attenuating neuroinflammation.

Objective:To explore the correlation between postoperative anxiety and emotional regulation difficulty and emotional reactivity in patients with osteoarthritis of the knee.Methods:A total of 155 patients with advanced osteoarthritis of the knee in Zhumadian Central Hospital of Henan Province from June 2017 to June 2018 were selected by the convenient sampling method. After the operation, patients filled in State-Trait Anxiety Inventory (STAI) , Emotional Reactivity Scale (ERS) and Difficulties in Emotion Regulation Scale (DERS) to analyze the correlations between emotional reactivity of the patients, emotional regulation difficulties and postoperative anxiety and explore the interaction between emotional regulation difficulty and emotional reactivity.Results:A total of 155 questionnaires were issued and 150 valid questionnaires were recovered, with an effective recovery rate of 96.77%. The STAI score of 150 patients was (122.69±6.49) , ERS score was (41.56±3.97) and DERS score was (72.82±5.61) . Postoperative anxiety was positively correlated with emotional reactivity and emotional regulation difficulty ( r=0.472, 0.296; P<0.05) . And the interaction of emotional regulation difficulty and emotional reactivity has an impact on postoperative anxiety level ( P<0.01) . Conclusions:The higher the levels of emotional reactivity and difficulty in emotional regulation in patients with osteoarthritis of the knee, the higher the level of postoperative anxiety in patients. And the higher the degree of interaction between emotional regulation difficulties and emotional reactivity, the greater the effect on postoperative anxiety.