Objective To investigate the pathological changes of nasal mucosa by intranasal acupuncture, and to explore the possible signal transduction mechanism through the distribution and expression of transient receptor potential vanillic acid receptor 1 (TRPV1)-substance P (SP) axis in nasal mucosa of experimental allergic rhinitis. Methods An animal model of allergic rhinitis was constructed by ovalbumin and aluminium hydroxide gel treatment. Twenty five New Zealand rabbits were randomly divided into normal group, model group, sham acupuncture group, acupuncture Wai Ying Xiang group and intranasal acupuncture group, with 5 rabbits in each group. To observe the effect of intranasal acupuncture on animal behavior, nasal mucosa pathology, eosinophils (EOS) count and IgE content in arterial blood. In addition, HE staining was used to determine the distribution of EOS in nasal mucosa and immunohistochemistry was used to observe the distribution and expression of TRPV1 and SP in nasal mucosa membrane. Results Compared with the model group, the behavioral score of the sham acupuncture group did not decrease significantly, while the behavioral score of the Wai Ying Xiang acupuncture group and the intranasal acupuncture group showed a downward trend, and the decrease degree of the intranasal acupuncture group was more significant. Compared with the normal group, the eosinophil count in the model group increased slightly (P<0.05); Compared with the model group, the number of eosinophils in the intranasal acupuncture group decreased (P<0.05). Compared with the normal group, the serum IgE content in the model group increased significantly (P<0.05). Compared with the model group, there was no significant change in the sham acupuncture group (P<0.05). Compared with the model group, serum IgE content in the Wai Ying Xiang acupuncture group and the internal acupuncture group decreased significantly (P<0.05), but there was no statistical difference between the internal acupuncture group and the Wai Ying Xiang acupuncture group (P>0.05). HE staining found that eosinophilic distribution increased significantly in the model group and the sham acupuncture group compared with the normal group, and the difference was statistically significant (P<0.05). Compared with the model group, the distribution of eosinophil reduced in the nasal acupuncture group and the Wai Ying Xiang group. The difference was statistically significant (P<0.05 ). Immunohistochemical found that TRPV1 and SP expressed significantly in the nasal mucosa of the model group and the sham acupuncture group compared with the normal group, the difference was statistically significant (P<0.05). Compared with the model group, TRPV1 and SP expression decreased significantly in the intranasal acupuncture group and the external yingxiang group, the difference was statistically significant (P<0.05). Conclusion Intranasal acupuncture can alleviate the nasal symptoms of allergic rhinitis model by reducing the Chemotaxis of eosinophils in nasal mucosa and the content of IgE in serum.

Objective To explore the influence of self-management education on the diabetes patients with depression with a view to improve patient self-management skills.Methods 85 cases were randomly divided into two groups: the experimental group ( n =43) and the control group ( n =42),general health knowledge education was applied to patients in control group,and patients in experimental group took part in the selfmanagement education activity,the status of self-management education and depression were tested by Zung' S Depression self-rating scale (SDS) and Hamilton Depression Rating Scale (24,HAMD),the self-management,depression,fasting plasma glucose (FPG),postprandial 2 h blood glucose (2 h PG) were compared between the two groups after eight weeks later.Results The score of self-management,SDS,HAMD,fasting plasma glucose and postprandial 2 h blood glucose were similar between the two groups before intervention ( P ＞ 0.05 ).After 8 weeks,the results of compliance of the self-management,manage of complications,SDS,HAMD,fasting plasma glucose,postprandial 2 h blood glucose were ( 1.77 ±0.504),(2.19 ±0.588),(29.3 ±4.8),( 11.5 ±6.9),(5.3 ± 1.4) and (8.6 ± 1.1 ) in experimental group,respectively; but were ( 1.32 ± 0.744),( 1.83 ± 0.590),(36.5 ± 5.4),( 18.3 ± 1.3 ),(9.2 ± 2.1 ),( 11.1 ± 2.2 ) in control group,there were significantly different between the two groups (t =2.813,3.358,2.91,2.34,2.65,2.78; P ＜ 0.01 ).another four items of selfmanagement were obviously higher than in control group (P ＜ 0.01 ).Conclusions Self-management education can improve the patients' ability of self-management,it also can control the blood glucose level and improve the depression status,it is worthy of spreading in clinical.

OBJECTIVETo assess the osseointegration capability of hydroxyapatite-coated porous titanium with bone morphogenetic protein-2 (BMP-2) and hyaluronic acid to repair defects in the distal femur metaphysis in rabbits.

METHODSPorous titanium implants were made by sintering titanium powder at high temperature, which were coated with hydroxyapatite by alkali and heat treatment and with BMP-2 combined with bone regeneration materials. And hyaluronic acid was further used as delivery system to prolong the effect of BMP-2. The implants were inserted into the metaphysis of the distal femur of rabbits. The animals were killed at 6, 12 and 24 weeks to accomplish histological and biomechanical analyses.

RESULTSAccording to the result of histological analysis, the osseointegration in BMP-2 group was better than that of the HA-coated porous titanium group. In push-out test, all the samples had bigger shear stress as time passed by. There was statistical difference between the two groups in 6 and 12 weeks but not in 24 weeks.

CONCLUSIONHydroxyapatite-coated porous titanium with BMP-2 and hyaluronic acid has a good effect in repairing defects of distal femur in rabbits, which is a fine biotechnology for future clinical application.

Animals ; Biomechanical Phenomena ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins ; pharmacology ; Coated Materials, Biocompatible ; Durapatite ; Femur ; surgery ; Hyaluronic Acid ; pharmacology ; Osseointegration ; physiology ; Porosity ; Prostheses and Implants ; Rabbits ; Titanium ; Transforming Growth Factor beta ; pharmacology

The degQ gene, amplified from Bacillus subtilis by PCR, was cloned to pUBS (sucrose induced secretion vector). After transformed into DB403, recombination named DB403(pUBSD) was formed. The results of the fermentation showed that degQ gene enhanced the expression of B. subtilis fibrin enzyme. The activity of the enzyme was increased to 2.2 times as the original one. In this article, the effects of different conditions, such as different kinds of sugar, different concentration of sucrose and different induced time were also be investigated and compared.