Objective To prepare isophenylcyclopentylamine hydrochloride capsules and evaluate its quality. Methods The suitable excipients were selected by the drug-excipient compatibility test,and after the formulation and preparation process design and screening,the micromeritic property and hygroscopicity of contents of capsules as well as the basic performance and stability of is-ophenylcyclopentylamine hydrochloride capsules were evaluate. Results The selected formulation was composed of isophenylcyclo-pentylamine hydrochloride 10 mg,anhydrous dibasic calcium phosphate 128.7 mg,mannitol 128.7 mg and aerosil 2.7 mg.The angle of repose of intermediate powders was(30.71±1.09)°,aerated and packed bulk densities were(0.76±0.01)and(0.90±0.02)g/ml re-spectively,Hausner value was 1.19±0.01,Carr′s index was(15.86±1.05)%,indicating good flowability and filling property as well as relatively low humidity.The contents,uniformity of contents and dissolution of capsules fulfilled the requirements.The quality of ca-pusles kept well in the 6-month accelerated and long-term stability test.Conclusion The formulation is simple and reasonable,and the preparation process showed a good reproducibility,which might be suitable for industrialization.

Objective To explore the effects of high birth weight (HBW) on blood pressure (BP) during childhood.Methods A total of 1435 couples with high or normal birth weight were selected from a birth cohort who were born between 1993 and 1995 in Wuxi,China and,followed between 2005 and 2007.A questionnaire survey was conducted,with their height,weight and blood pressure measured.Results The medians of systolic blood pressure (SBP) and diastolic BP (DBP)of HBW group were 100 mm Hg (Q1-Q3∶90-110 mm Hg),64 mm Hg (Q1-Q3∶60-70 mm Hg) in the high birth weight group,respectively,while they were 100 mm Hg (Q1-Q3∶90-108 mm Hg),62 mm Hg (Q1-Q3∶60-70 mm Hg) in the normal weight group.No statistical significant difference was found between the exposed and unexposed groups.The proportion of elevated BP,including prehypertension and hypertension,in exposed group was 13.66％,a bit higher than that (11.57％) of the unexposed group (P ＝ 0.055).The relative risk (RR) of HBW on elevated BP was 1.06 (0.92-1.21),after adjusted the following parameters as BMI in childhood,sex,age during the follow-up period,age of mother at delivery,gestational age,type of feeding at infancy,dietary habit as well as the frequencies of exercise during childhood.Conclusion No statistically significant correlation was found between high birth weight and blood pressure of children.

Objective To explore the effect and heaviness of smoking on the routine parameters of semen quality.Methods A total of 727 eligible subjects including 421 smokers and 306 nonsmokers were selected in 2004-2006 in Hebei,Shanxi,Guizhou,Zhejiang and Shandong provinces.Data on background information,general demographic characteristics,exposure to smoking and related confounding factors were obtained through a questionnaire survey.Semen samples of all the subjects were collected during the period of study.Parameters of semen were examined according to the manual recommended by WHO.Results Four models from the multiple logistic regression regarding the semen routine parameters were used as the dependent variables to be fitted in descending order respectively,while six confounders including area,age,abstinence time,education level,history of alcohol intake and the exposure to pesticides atc.,were adjusted.Results showed that smoking could affect the sperm viability,with significantly higher proportion of abnormal sperm viability seen in the smoking group (OR= 1.77,95％ CI:1.12-2.80).Proportions regarding the abnormal mobility and morphology of sperms in the smoking group were also significantly higher than in the control group,with OR values as 1.51 (95％CI:1.00-2.27) and 2.55 (95％CI:1.68-3.88) respectively.The density of sperms was significantly decreased in the smoking groups who smoked more than 20 cigarettes per day (OR= 1.70,95％CI:1.05-2.76) or the total numbers of cigarette consumption exceeding 150 000(OR=1.84,95％CI:1.03-3.29).Conclusion Smoking showed adverse effects on the quality of semens,especially for heavy or long-term smokers.

Objective To evaluate the possible association between RFC-1 polymorphism and cervix carcinoma.as well as the interaction between polymorphism and human papilloma virusl6(HPV16).Methods Based on a hospital-based case-control study.107 cases which were diagnosed as cervical cancer pathematologically and 107 controls with hysteromyoma,were selected by frequency,matched with age and habitation.HPV16 and RFC-1 A80G polymorphism were detected by special PCR and RFLP Results (1)HPV16 infection rate in CaseS(56.07%)Was higherthan that in controls(31.78%)with the adjusted OR with RFC-1 AA,RFC-1 GG had higher risk for cervical cancer with OR of2.42(95%CI:1.01-5.81).(4)No statistical significance was noticed regarding the interaction between RFC-l polymorphism and HPV16 in logistic regression method.Conclusion The introduction of RFC-1 80GG gene type could increase the risk of cervical cancer.

e quality of semen.

Aspergillosis ; diagnosis ; microbiology ; Aspergillus fumigatus ; genetics ; isolation & purification ; DNA, Fungal ; genetics ; isolation & purification ; Dermatomycoses ; diagnosis ; microbiology ; Eye Diseases ; diagnosis ; microbiology ; Female ; Humans ; Middle Aged ; Polymerase Chain Reaction

Adult ; Base Sequence ; Biopsy ; Chlamydia Infections ; complications ; Chlamydia trachomatis ; genetics ; isolation & purification ; Female ; Humans ; Lymph Nodes ; pathology ; Lymphogranuloma Venereum ; etiology ; Molecular Sequence Data ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length

AIMTo examine whether reproductive hormones play a role in the association between body mass index (BMI) and semen quality.

METHODSSemen quality and testosterone (T), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and estradiol (E(2)) were evaluated in 990 fertile males with age 38.9 +/- 9.7 (mean +/- SD) years recruited from the Chinese general population in 2001 and 2002.

RESULTSSemen quality was reduced among underweight (BMI < 18.5) compared with normal (BMI 18.5-24.9) and overweight (BMI 25.0-29.9), but the associations were independent of reproductive hormones. After adjustment for the potential confounders, underweight men had reductions in sperm concentration (22.4 X 10(6)/mL), total sperm count (52.9 X 10(6)) and percentage of normal sperm forms (6.9%) compared with men with normal BMI. Being underweight may be a risk factor for low sperm concentration (OR: 4.68, 95% confidence intervals [CI]: 2.01-10.91). Otherwise, being overweight may be a protected factor for low sperm concentration (OR: 0.25; 95% CI: 0.08-0.83) and low total sperm count (OR: 0.37, 95% CI: 0.15-0.87).

CONCLUSIONLow BMI was associated with reduced semen quality. The associations between BMI and semen quality were found statistically significant even after adjustment for reproductive hormones. Reproductive hormones cannot explain the association between BMI and semen quality.

Adult ; Body Mass Index ; Body Weight ; physiology ; China ; Estradiol ; blood ; Fertility ; physiology ; Follicle Stimulating Hormone ; blood ; Humans ; Luteinizing Hormone ; blood ; Male ; Middle Aged ; Semen ; physiology ; Sperm Count ; Testosterone ; blood

OBJECTIVETo evaluate the roles of expression and early protein E2 and E6 load of human papillomavirus type 16 (HPV16) on cervical cancer in order to explore the relation between disruption of E2 and development of cervical cancer.

METHODSA case-control study was conducted, including 141 cervical cancer patients as cases who had been diagnosed by cytological approaches and histological approaches in Shanxi province Tumor Hospital, China. Two type of controls including 137 hospital controls with hysteromyoma by cytology or histology and eligible 129 controls from 1582 healthy women in the community who took part in community-organized physical examination with neither CIN2-3 nor invasive cancer, nor other gynecologic diseases were recruited. HPV16 E2 and E6 oncogenes were detected by multiple polymerase chain reaction (multi-PCR). The levels of E2 and E6 were analyzed used Bio-1D+ + software provided by VILBER pattern formatter.

RESULTSThe positive rates of HPV16 E6 in cancer cases (46.8%) were significantly higher than that in hysteromyoma group (24.1%) or healthy control group (2.3%) and accounted for 2.77 of OR (95% CI: 1.66-4.63) and 36.96 of OR(95% CI: 11.22-121.71) respectively. The expressions and loads of HPV16 E6 and E2 in cases were significantly higher than that in two control groups. Meanwhile, the expression or level of E6 was higher than that of E2 in each group. Disruption rate of E2 was 22.73% and the ratio of E6 to E2 was 1.24 in cervical cancer group.

CONCLUSIONThe positive rates and levels of HPV16 E6 or E2 found in cervical cancer were higher than that in hysteromyoma and healthy women. High expression of E6 and disruption of E2 might play an important role in the development of HPV-induced cervical cancer.

Adult ; Aged ; Case-Control Studies ; DNA, Viral ; genetics ; DNA-Binding Proteins ; genetics ; Female ; Gene Expression Regulation, Viral ; Human papillomavirus 16 ; genetics ; Humans ; Middle Aged ; Oncogene Proteins, Viral ; genetics ; Papillomavirus Infections ; genetics ; virology ; Polymerase Chain Reaction ; Repressor Proteins ; genetics ; Uterine Cervical Neoplasms ; genetics ; virology

UNLABELLEDStudy on the effect of a community-based early education and service program regarding intelligence

OBJECTIVETo assess the effect on a community-based early education and service programs regarding the development of infants' intelligence.

METHODSA community-based intervention study was carried out among 359 infants and their families. Base-line survey were carried out when infants reached their one month, where after infants and their families in the intervention group received instructions and services focused on baby fostering and intelligence development. When the infants reached their six-months and twelve-months of age, their families were informed to complete the follow-up surveys, using both questionnaire investigation and testing the infants' intelligence quotients. The infants' intelligence quotients were measured by Development Screening Test for children under six. By comparing intelligence quotients of infants in two study groups in the follow-up surveys, this paper evaluated the impacts of community intervention on the infants' intelligence development.

RESULTSDuring two follow-ups, no statistical difference had been detected between the two groups of infants in term of gender or delivery process. Baseline data showed that infants' mental index (MI) scored 98.26 in the intervention group and 101.79 in the control one, and development quotient (DQ) scored 94.50 and 99.36 in respective groups. Infants' MI score increased 6.07 and 8.86 at the six-month and twelve-month follow-up periods respectively in the intervention group compared during the baseline, higher than the MI increments of the control group at the two follow-up periods (-2.46 and 1.05 respectively). DQ score of infants in the intervention group increased 12.94 and 11.24 respectively in the two follow-up surveys, which were also higher than increments in the control group (-0.18 and 0.34). The group x time effect(interaction effect) of MI and DQ in six-month and twelve-month follow-ups were both significantly higher than that of the baseline level.

CONCLUSIONThe community-based early education and service programs could effectively improve the infants' intelligence.

Child Development ; Community Health Services ; Female ; Humans ; Infant ; Intelligence ; Male