Frailty is a complex age-related clinical condition characterized by a decline in physiological capacity across several organ systems, with a resultant increased susceptibility to stressors.The relationship between frailty assessment and elderly patients’ safety during perioperative period has received widespread attention.This paper reviews research progress of frailty assessment in elderly patients during perioperative period in the department of urology.

Hypertensive disorder of pregnancy (HDP) involves two major public health issues: mother-infant safety and prevention and controlling major chronic disease. HDP poses a serious threat to maternal and neonatal safety, and it is one of the leading causes of maternal and perinatal morbidity and mortality worldwide, as well as an important risk factor for long-term cardiovascular disease (CVD). In order to explore effective strategies to prevent and control the source of CVD and reduce its risk, we have established a cohort of HDPs in Shenzhen for the primordial prevention of CVD. The construction of the HDP cohort has already achieved preliminary progress till now. A total of 2 239 HDP women have been recruited in the HDP cohort. We have established a cohort data management platform and Biobank. The follow-up and assessment of postpartum cardiovascular metabolic risk in this cohort has also been launched. Our efforts will help explore the pathophysiological mechanism of HDP, especially the pathogenesis and precision phenotyping, prediction, and prevention of pre-eclampsia, which, therefore, may reduce the risk of adverse pregnancy outcomes, and provide a bridge to linking HDP and maternal-neonatal cardiovascular, metabolic risk to promote the cardiovascular health of mothers and their infants.

Objective:To understand the supportive care needs of patients with prostate cancer during their illness, so as to construct the supportive care needs intervention program.Methods:Purposive sampling method was used to conduct semi-structured interviews on 17 patients with prostate cancer from June 2019 to January 2020 in Haikou Hospital of Xiangya Affiliated Hospital of Central South University, and the data were analyzed by content analysis method.Results:Seven themes were extracted: the need of health information, the need of social support, the need of health care services, psychological and emotional need, the need of symptom management, the need of economic support and disease specific need.Conclusions:Patients with prostate cancer have multiple supportive care needs. The results of this study could be important reference for medical staff to provide comprehensive, personalized and humanistic care for patients and improve patients′ the quality of life and medical experience.

In the special period of COVID-19’s epidemic situation, plastic surgeons are subordinate first and foremost to the national situation and assume the duties that doctors have to cope with in the fight against the epidemic. At the same time, conditions have been created for the provision of professional services to patients who have to undergo emergency plastic treatment, while ensuring the safety of the epidemic. The forum has two components: (1) Presentation of the experience of representative medical institutions in the emergency plastic treatment in a safe manner to prevent epidemics and the safety measures for plastic work in the normal environment of epidemic prevention. (2) A reflection by the plastic surgeons on the professional characteristics and problems at this extraordinary time, and a discussion of the factors affecting the future of plastic surgery industry.

In the special period of COVID-19’s epidemic situation, plastic surgeons are subordinate first and foremost to the national situation and assume the duties that doctors have to cope with in the fight against the epidemic. At the same time, conditions have been created for the provision of professional services to patients who have to undergo emergency plastic treatment, while ensuring the safety of the epidemic. The forum has two components: (1) Presentation of the experience of representative medical institutions in the emergency plastic treatment in a safe manner to prevent epidemics and the safety measures for plastic work in the normal environment of epidemic prevention. (2) A reflection by the plastic surgeons on the professional characteristics and problems at this extraordinary time, and a discussion of the factors affecting the future of plastic surgery industry.

OBJECTIVE: To investigate the effects of ribonucleotide reductase catalytic subunit M1 (RRM1) gene silencing on drug resistance of human breast cancer cell line MCF-7/R. METHODS: We established a paclitaxel-resistant breast cancer MCF-7 cell line (MCF-7/R) by exposing the cells to high-concentration paclitaxel in a short time. Small interfering RNAs (siRNAs) targeting RRM1 were designed to silence RRM1 expression in human breast cancer MCF-7/R cells. MTT assay was used to detect the IC values and the sensitivity to paclitaxel in the cells with or without siRNA transfection. The changes in the proliferative activity of MCF7 and MCF-7/R cells following RRM1 gene silencing were evaluated using EdU assay. Flow cytometry was used to analyze the cell apoptosis and cell cycle changes. We assessed the effect of RRM1 gene silencing and paclitaxel on the tumor growth in a nude mouse model bearing subcutaneous xenografts with or without siRNA transfection. RESULTS: We detected significantly higher expressions of RRM1 at both the mRNA and protein levels in the drug-resistant MCF- 7/R cells than in the parental MCF-7 cells ( < 0.01). Transfection with the specific siRNAs significantly reduced the expression of RRM1 in MCF-7/R cells ( < 0.05), which showed a significantly lower IC value of paclitaxel than the cells transfected with the negative control siRNA ( < 0.05). RRM1 silencing significantly inhibited the proliferation ( < 0.01) and enhanced the apoptosis-inducing effect of paclitaxel in MCF-7/R cells ( < 0.001); RRM1 silencing also resulted in obviously reduced Akt phosphorylation, suppressed Bcl-2 expression and promoted the expression of p53 protein in MCF-7/R cells. In the tumor-bearing nude mice, the volume of subcutaneously transplanted tumors was significantly smaller in MCF-7/R/siRNA+ PTX group than in the other groups ( < 0.001). CONCLUSIONS RRM1 gene silencing can reverse paclitaxel resistance in human breast cancer cell line MCF-7/R by promoting cell apoptosis.
Animals ; Apoptosis ; Breast Neoplasms ; Drug Resistance, Neoplasm ; Gene Silencing ; Humans ; MCF-7 Cells ; Mice ; Mice, Nude ; Paclitaxel ; RNA, Small Interfering ; Ribonucleotide Reductases ; Tumor Suppressor Proteins

With the rapid development of organ transplantation in China,the donation after cardiac death (DCD) donor organs are widely used.However,the quality of these organs is relatively poor,so the way to preserve and maintain organ still remains a severe problem.Among them,ischemic reperfusion injury (IRI) impairs the organs severely.Acetaldehyde dehydrogenase 2 (ALDH2) protects organs from stress conditions,including ischemia-reperfusion injury,and the activation and autophagy inhibition also protects the organs from stress conditions as well.Recent studies showed that ALDH2 can regulate autophagy to inhibit the organ injury during ischemia-reperfusion.Our study aims to discuss the new findings in this mechanism.

The paper introduces the common methods for automatic de-identification of clinical texts,including the method based on rules,machine learning method,comprehensive method,etc.,states the challenges for clinical texts practicability,system universality and scalability of clinical texts de-identification research,analyzes the further research direction,and provides reference for researchers of this field.

Objective To study the influence of the combined administration of Bletilla striata and Radix aconiti preparata with different doses or ratios on anhydrous ethanol-induced gastric mucosal injury in mice with gastric ulcer. Methods The uniform design method was adopted on the basis of two factors and seven levels, which aims to investigate the influence of combined oral administration of Bletilla striata and Radix aconiti preparata with different doses or ratios on gastric mucosal injury in the mice with gastric ulcer. The indices like gastric ulcer index and stomach mucosa damage index were used to help selecting the right dose or ratio of decoctions for further study. Results The combined administration of Bletilla striata and Radix aconiti preparata at a certain proportion could reduce gastric ulcer index and stomach mucosa damage index. According to the regression analysis, the gastric ulcer index and stomach mucosa damage index were obviously inhibited by Bletilla striata, while this function decreased with the combined administration of Bletilla striata and Radix aconiti preparata. However, an interactive effect between each other has not been found. The influence of decoctions on the gastric ulcer index and stomach mucosa damage index became less when the compatibility ratio of Radix aconiti preparata increased, which referred to the total dose ranged between 3.64 g/kg and 29.32 g/kg. Conclusions The effect of the combined administration of Bletilla striata and Radix aconiti preparata showed relationship among the doses and ratios on anhydrous ethanol-induced gastric mucosal injury in mice with gastric ulcer.

Objective To investigate the expression levels of adiponectin and visfatin in the gingival crevicular fluid (GCF) and serum of patients with obesity and chronic periodontitis.Methods The study included 80 patients.Among them,20 were healthy controls (H group),20 had periodontitis (CP group),20 had obesity (OB group),and 20 had both obesity and periodontitis (OB+CP group).The patients' baseline information and clinical parameters were recorded,and the GCF and serum were collected.The concentration of adiponectin and visfatin in the GCF and serum were then assayed using ELISA.Use the SPSS 17.0 software package for statistical analysis.Results The concentration of adiponectin in the serum of the CP group,the OB group,and the OB+CP group were significantly lower than that of the H group (P ＜ 0.05).The concentration of adiponectin in the serum of the OB+CP group was lower than that of both the CP and OB groups (P ＜ 0.05).The concentration of adiponectin in the GCF of the CP group,the OB group,and the H group were higher than that of the OB+CP group (P ＜ 0.05).The concentration of adiponectin in the GCF of the CP group,the OB group,and the H group were lower than that of the OB+CP group (P ＜ 0.05).Conclusion Obesity may aggravate the destruction of periodontal tissue by inhibiting the expression of adiponectin and promoting the expression of visfatin.