Chondroblastoma ; pathology ; Chondroma ; pathology ; Chondrosarcoma ; metabolism ; pathology ; surgery ; Cricoid Cartilage ; Diagnosis, Differential ; Humans ; Laryngeal Neoplasms ; metabolism ; pathology ; surgery ; Laryngectomy ; Lymph Node Excision ; Male ; Middle Aged ; Osteoblastoma ; pathology ; Osteosarcoma ; pathology ; S100 Proteins ; metabolism ; Sarcoma, Clear Cell ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Asthma ; diagnosis ; Bronchial Neoplasms ; diagnosis ; Child ; Diagnosis, Differential ; Female ; Humans ; Lymphoma, Large-Cell, Anaplastic ; diagnosis ; Tracheal Neoplasms ; diagnosis

Objective: To explore the sensitivity of Kit or PDGFRA mutants related to gastrointestinal stromal tumor (GIST) to Gleevec.Methods: The recombinant plasmids of KIT Del559-560, KIT Ins IPYD579, PDGFRA D842V and PDG-FRA L839P gene mutants were transiently transformed into the CHO cells by liposome methods.Western blot was used to detect the expression of the related protein and their phosphorylated forms after the cells were incubated with Gleevec for 90 min.At 72 hours after incubation with Gleevec, MTT was used to detect cell proliferation.Results: Western blot results showed that Gleevec at 0.1 μM can notably reduce phosphorylation of KIT Del559-560.Gleevec at 1μM completely blocked phosphorylation of KIT Ins IPYD579 and PDGFRA L839P, but did not affect PDGFRA D842V phosphorylation.MTT analy-sis indicated that growth of CHOPDGFRA L839P was inhibited by Gleevec at 1μM, however, CHOPDGFRA D842V was re-sistant to Gleevec at 5 μM.Conclusion: Gleevec can decrease the expression of phosphorylated protein CHOPDGFRA L839P and CHOKIT Ins IPYD579, and can remarkably inhibit the proliferation of cells containing PDGFRA L839P mutant.

OBJECTIVETo investigate the clinicopathological and prognostic significance of the c-kit protein in gastrointestinal stromal tumor (GIST).

METHODSParaffin embedded materials from 53 benign GISTs, 13 potentially malignant and 55 malignant cases were analysed for c-kit expression by immunohistochemical method, while using leiomyomas and schwannomas as controls. Positive signals were shown in cytoplasma and cell membrane.

RESULTSOf 122 GISTs, 118 (97%) were positive for c-kit. Localization of positive signals was accurate. The rate of c-kit protein in benign, potentially malignant and malignant cases was 98% (53/54), 93% (12/13), 96% (53/55) respectively. Compared with benign GIST, the positivity of c-kit in metastasis or recurrent cases decreased, but c-kit protein expression rate was not significantly different between the three patterns of GIST (chi(2) = 1.167, P > 0.05). Leiomyomas and schwannomas were typically c-kit negative.

CONCLUSIONAs a sensitive and specific marker of GIST, c-kit seems to be a useful antibody in the diagnosis and differential diagnosis of GIST, but it may not be used as a prognostic index.

Biomarkers, Tumor ; biosynthesis ; genetics ; Diagnosis, Differential ; Gastrointestinal Neoplasms ; diagnosis ; metabolism ; Humans ; Immunohistochemistry ; Mutation ; Prognosis ; Proto-Oncogene Proteins c-kit ; biosynthesis ; genetics

OBJECTIVETo investigate the effect of the expression and mutation of c-kit gene and its relationship with clinical pathology and prognosis of gastrointestinal stromal tumor (GIST).

METHODSImmunohistochemical and PCR-SSCP techniques were used to detect c-kit protein expression and c-kit gene exon 11 mutation in 82 patients with GIST.

RESULTSThe positive c-kit protein expression and c-kit gene mutation rates were 97.6% (80/82) and 41.5% (34/82). Correlating the results of these two methods and clinicopathological factors, the c-kit expression and c-kit gene mutation rates were 95.0% (19/20) and 0 in benign GIST, and were 98.4% (61/62), 54.8% (34/62) in malignant GIST. Mutation positive GIST showed higher frequency of adjacent tissue invasion, metastasis and recurrence as compared with mutation negative ones.

CONCLUSIONc-kit protein is an important diagnostic marker of gastrointestinal stromal tumor. c-kit gene mutation may play a significant role in the pathogenesis of GIST and also may be a prognostic marker.

Adolescent ; Adult ; Aged ; Child ; Female ; Gastrointestinal Neoplasms ; diagnosis ; genetics ; pathology ; Humans ; Male ; Middle Aged ; Mutation ; Neoplasm Metastasis ; Polymerase Chain Reaction ; Polymorphism, Single-Stranded Conformational ; Prognosis ; Proto-Oncogene Proteins c-kit ; genetics

Objective: To observe the effect of coxsackie virus B3 on airway tract and lung morphology, and to study the relation between CVB infection and asthma. Methods: We established CVB3 infective model: 5 d neonatal rats inhaled CVB3 by ultrasonic brume. CVB3-IgM was examined 10 d after inoculating of CVB3, and LW/BW, airway tract and lung pathological change 10 d and 30 d after inoculation of CVB3 were observed. Results: Rats from the virus group had higher D of CVB3-IgM than control's (+2s ) and had higher LW/BW 10 d after inoculation of CVB3 than control (P<0.01). Neonatal rats had acute inflammatory changes 10 d after inoculation of CVB3 and persistent changes in morphology and cytology. Conclusion: Neonatal rats virus model is established. Respiratory infection by CVB3 in neonatal rats has persistent changes in airway tract inflammatory and morphology.

Objective: To study the expression of CD15 mRNA,CD44v6 mRNA and nm23H1 mRNA in colon carcinoma and its relationship with clinical pathological parameters and prognosis of colon carcinoma. Methods: In situ hybridization was used to detect the expression of CD15 mRNA, CD44v6 mRNA and nm23H1 mRNA in 90 cases of colon carcinoma, and 53 cases were followed up for more than 5 years. Results: In 90 cases of colon carcinoma, the expression of CD15 mRNA,CD44v6 mRNA and nm23H1 mRNA were 84.4%,68.9%,66.7%, respectively. The high level expression of CD15 mRNA, CD44v6 mRNA and the low level expression of nm23H1 mRNA were positively corelated with the Dukes staging,serosa infiltration,lymph node and liver metastasis of colon carcinoma.The expression of CD15 mRNA in colon carcinoma was positively associated with that of CD44v6 mRNA and negatively with that of nm23H1 mRNA. Conclusion: CD15 mRNA,CD44v6 mRNA and nm23H1 mRNA play an up-regulation and a down-regulation role, respectively, and have a synergistic action in metastasis of colon carcinoma. CD15 mRNA can be used as a new biological index to predict the invasive potential of colon carcinoma and objectively evaluate the prognosis of patients.

Objective To analyse the difference of IgG expressions between fetal and adult rabbit skin in wound healing for exploring the mechanisms of fetal-type and adult-type wound healing. Methods Based on a fetal rabbit wound healing model, we extracted IgG from normal and wounded tissues using the homogenization method and determined its value using ELISA technique in the comparative study. Results We found that the variation of IgG showed a tendency of elevation with the prolonging of pregnancy in normal rabbits(23-28 days gestation);the IgG value in adult rabbits was much higher than that of the fetuses(P＜0.01);IgG value in wounded fetuses was higher(P＜0.01) than that of corresponding normal fetuses during the first two days after wound creation, and there were no differences between wounded fetuses at different gestation times. From the 2nd day of wound creation, IgG value in wounded adults was much higher than the normal(P＜0.01) and had an obvious tendency of elevation. All the facts suggested that the immunological system became mature with the development of the fetus. Despite the lower IgG levels in the fetus,it also manifested some immunological reactions to injury whereas the adult took intense reactions with higher IgG value and an obvious tendency of elevation. Conclsion The difference of immunoreaction is one of the main reasons of the fetal-type and adult-type wound healin

Objective To analyse the difference of IgG expressions between fetal and adult rabbit skin in wound healing for exploring the mechanisms of fetal-type and adult-type wound healing. Methods Based on a fetal rabbit wound healing model, we extracted IgG from normal and wounded tissues using the homogenization method and determined its value using ELISA technique in the comparative study. Results We found that the variation of IgG showed a tendency of elevation with the prolonging of pregnancy in normal rabbits(23-28 days gestation);the IgG value in adult rabbits was much higher than that of the fetuses(P＜0.01);IgG value in wounded fetuses was higher(P＜0.01) than that of corresponding normal fetuses during the first two days after wound creation, and there were no differences between wounded fetuses at different gestation times. From the 2nd day of wound creation, IgG value in wounded adults was much higher than the normal(P＜0.01) and had an obvious tendency of elevation. All the facts suggested that the immunological system became mature with the development of the fetus. Despite the lower IgG levels in the fetus,it also manifested some immunological reactions to injury whereas the adult took intense reactions with higher IgG value and an obvious tendency of elevation. Conclsion The difference of immunoreaction is one of the main reasons of the fetal-type and adult-type wound healin