Interleukin (IL)-17A, a pro-inflammatory cytokine, is a fundamental function in the onset and advancement of multiple immune diseases. To uncover the primary compounds with IL-17A inhibitory activity, a large-scale screening of the library of traditional Chinese medicine constituents and microbial secondary metabolites was conducted using splenic cells from IL-17A-GFP reporter mice cultured under Th17-priming conditions. Our results indicated that some aureane-type sesquiterpene tetraketides isolated from a wetland mud-derived fungus, Myrothecium gramineum, showed remarkable IL-17A inhibitory activity. Nine new aureane-type sesquiterpene tetraketides, myrogramins A-I ( 1, 4- 11), and two known ones ( 2 and 3) were isolated and identified from the strain. Compounds 1, 3, 4, 10, and 11 exhibited significant IL-17A inhibitory activity. Among them, compound 3, with a high fermentation yield dose-dependently inhibited the generation of IL-17A and suppressed glycolysis in splenic cells under Th17-priming conditions. Strikingly, compound 3 suppressed immunopathology in both IL-17A-mediated animal models of experimental autoimmune encephalomyelitis and pulmonary hypertension. Our results revealed that aureane-type sesquiterpene tetraketides are a novel class of immunomodulators with IL-17A inhibitory activity, and hold great promise applications in treating IL-17A-mediated immune diseases.

ObjectiveTo analyze the association between alcohol dependence and overweight and obesity among high school students. MethodsThis study adopted stratified cluster sampling method. Four high schools （one vocational high school included） were selected as the research sites. We used self-designed questionnaires to investigate the drinking behavior of high school students. This study used propensity score matching method to control confounding factors （e.g gender， school type， etc.）. Then we compared the differences in drinking behavior between obese/overweight group and normal weight group after matching， and used a multivariate logistic regression model to analyze the relationship between drinking behavior and overweight and obesity. ResultsA total of 811 high school students participated in the survey. 60 people were excluded due to mild and moderate weight loss， and 751 people were normal or obese/overweight. After 1：1 propensity score matching， there were 217 people each in obese/overweight group and normal group. The characteristics of gender， school type， grade and family economic level of the two groups were balanced after matching. The difference was statistically significant （P<0.05） between obese/overweight and normal group in the following： drinking of alcohol at high school， alcohol dependence， the maximum drinks at one time， drinking of alcohol at family gatherings， whether bought alcohol for others， whether their family members recommended drinking， whether they have tried to quit drinking in the past year， and the time of blush after drinking. The multivariate logistic regression analysis indicated that the degree of alcohol dependence （the alcohol use disorder score ≥ 8） was a risk factor for overweight and obesity （OR=3.600， 95%CI： 1.244‒10.424）. Besides， having bought alcohol for others and family members advising alcohol consumption were suggested to be protective factors for overweight and obesity （OR=0.525， 95%CI： 0.309‒0.890 and OR=0.377， 95%CI： 0.222‒0.641）. ConclusionHigh school students who have alcohol dependence suffer a higher risk of obesity/overweight， while high school students who may have drinking behaviors but do not have alcohol dependence have a lower risk of obesity/overweight. It is critical to guide young people on drinking in terms of health.

ObjectiveTo investigate the alcohol drinking among high school students， determine the possible influencing factors， and provide scientific evidence for effective control of alcohol drinking among high school students. MethodsThis study adopted stratified cluster sampling and included 4 high schools in Changning District （including one vocational high school）. Self-designed questionnaires were used to investigate alcohol drinking related knowledge and behavior among high school students. The questionnaire included demographics， alcohol drinking frequency and scenarios. SPSS 20.0 was used for Chi-square test and multivariate logistic regression analysis. ResultsThere were 811 high school students included in this study. The proportion of ever alcohol drinking was determined to be 48.09% （n=390） among students. Furthermore， 36.13% （n=293） reported alcohol drinking in the past year， and 14.80% （n=120） reported drinking in the past month. The proportion of excessive alcohol drinking was 8.26% （n=67） and that of alcohol dependence was 3.58% （n=29）. Moreover， the proportion of Chinese Baijiu （Chinese liquor） drinking in the past month was significantly higher than that in the past year （P<0.05）. In contrast， the proportions of wine and rice wine drinking in the past month was significantly lower than those in the past year （P<0.05）. The logistic regression model suggested no alcohol drinking in the past 30 days， parents disagreeing to their children’s drinking， some of friends having alcohol drinking， higher frequency of attending friend gatherings， and smoking were significantly associated with alcohol drinking among the students. ConclusionAlcohol drinking is moderately prevalent among high school students. Possible influencing factors may be correlated to their parents and friends. It warrants targeted intervention measures to prevent and control the early alcohol drinking among adolescents to avert alcohol dependence.

Objective: To analyze the relationship between alcohol related knowledge, attitude,practice and alcohol use disorder among high school students, and to provide reference for alcohol abuse intervention. Methods: The study adopted stratified cluster sampling. A total of 811 high school students from 4 high schools in Changning District were selected to conduct a questionnaire survey on alcohol related knowledge, attitude,practice and alcohol dependence. The software SPSS 20.0 was used for statistical analysis. Results: There were 279 (34.4%) high school students with mild alcohol use disorder and 29 (3.6%) with severe alcohol use disorder. The average score of high school students alcohol related knowledge, attitude,practice scores were (9.56±3.55) ( 4.96± 2.36) and (2.81±1.29),respectively. High school students alcohol related knowledge, attitude,practice were negatively correlated with alcohol use disorder score ( r =-0.10, -0.39, -0.71, P <0.01). Multivariate Logistic regression indicated that the total score of alcohol related KAP ( OR=0.86, 95%CI =0.83-0.89) and the family economic level (high level: OR=2.05, 95%CI =1.26-3.32) were positively associated with mild alcohol use disorder. The total score of alcohol related KAP ( OR=0.76, 95%CI =0.70-0.83) and school type ( OR=3.72, 95%CI =1.51-9.18) were positively associated with severe alcohol use disorder ( P <0.05). Conclusion There is a correlation between low alcohol related KAP and alcohol use disorder, alcohol related health education should be strengthened, especially among vocational school students and students from high family economic level.

To investigate the temporal trend of antibiotic use among children in Shanghai from 2017 to 2020. The stratified cluster sampling method was used to establish a dynamic cohort of healthy children based on primary schools in Changning District, Shanghai. In the cohort, there were 282 children from 2017, 287 children from 2018, 294 from 2019 and 301 from 2020. A total of 700 children aged 7-11 years were included in the study. The basic information and antibiotic use of children were investigated by questionnaire every year, and their height and weight were measured at the same time. Chi-square test was used to analyze the difference of antibiotic use rate in each year and generalized estimation equation was used to analyze the temporal trend of antibiotic use. The results showed that the use rates of all antibiotics, cephalosporins, azithromycin and other antibiotics (including penicillin, lincomycin, quinolones, etc.) of children between 2017 and 2020 were 15.6%, 10.5%, 2.7%, and 2.4%, respectively. In 2017, 2018, 2019, and 2020, there were significant differences for the use rates of total antibiotics and other antibiotics in children ( P=0.033, P=0.040), and there were no significant differences for the use rates of cephalosporins and azithromycin ( P=0.274, P=0.455). After adjusting for children′s basic characteristics, the generalized estimation equation showed that the annual use rate of all antibiotics, cephalosporins, and other antibiotics decreased over time.

To investigate the temporal trend of antibiotic use among children in Shanghai from 2017 to 2020. The stratified cluster sampling method was used to establish a dynamic cohort of healthy children based on primary schools in Changning District, Shanghai. In the cohort, there were 282 children from 2017, 287 children from 2018, 294 from 2019 and 301 from 2020. A total of 700 children aged 7-11 years were included in the study. The basic information and antibiotic use of children were investigated by questionnaire every year, and their height and weight were measured at the same time. Chi-square test was used to analyze the difference of antibiotic use rate in each year and generalized estimation equation was used to analyze the temporal trend of antibiotic use. The results showed that the use rates of all antibiotics, cephalosporins, azithromycin and other antibiotics (including penicillin, lincomycin, quinolones, etc.) of children between 2017 and 2020 were 15.6%, 10.5%, 2.7%, and 2.4%, respectively. In 2017, 2018, 2019, and 2020, there were significant differences for the use rates of total antibiotics and other antibiotics in children ( P=0.033, P=0.040), and there were no significant differences for the use rates of cephalosporins and azithromycin ( P=0.274, P=0.455). After adjusting for children′s basic characteristics, the generalized estimation equation showed that the annual use rate of all antibiotics, cephalosporins, and other antibiotics decreased over time.

Organic carbonates (OCs) are a class of compounds featured by a carbonyl flanked by two alkoxy/aryloxy groups. They exist in either linear or cyclic forms, of which the majority encountered in nature adopt a pentacyclic structure. However, the enzymatic basis for pentacyclic carbonate ring formation remains elusive. Here, we reported that a four-protein metabolon (AlmUII-UV) assembled by a small peptide protein (AlmUV) appends a reactive

Objective To explore the indoor air pollution level related to traffic and fuel gas using for cooking and to assess its effect on the respiratory disease and symptoms in children and teenagers. Methods Based on a cross-sectional survey in 6 551 children and teenagers in the urban area of Shanghai in 2007, 197 children were randomly selected in stratified districts and the level of CO, NO2, PM10 and benzene in indoor air in their home were measured. The variables of distance of nearest street or way, traffic density and the time of using fuel gas for cooking were collected and a multiple linear regression model was established to estimate indoor air pollution level related traffic and fuel gas using for cooking. The associations between indoor air pollutants concentrations and respiratory symptoms and disease were analyzed with multiple Logistic Regression model. Results After potential founders being controlled, the significant associations were observed between per 20 ?g/m3 increase of indoor NO2 concentration and dry cough(OR:1.12,95%CI:1.03-1.20),morning cough(OR:1.08,95%CI:1.01-1.17), wheezing (OR: 1.09,95%CI:1.0-1.19), bronchitis (OR: 1.21, 95%CI:1.01-1.46). The similar effects were observed between the per 30 ?g/m3 increase of indoor PM10 concentration and dry cough(OR:1.28,95%CI:1.02-1.59),wheeze(OR:1.34,95%CI:1.04-1.71),current asthma (OR:1.87,95%CI:1.08-3.23). The significant associations were also observed between house was nearby major street/road (